ATE173018T1 - Herstellung von fusionsproteinen - Google Patents

Herstellung von fusionsproteinen

Info

Publication number
ATE173018T1
ATE173018T1 AT90912715T AT90912715T ATE173018T1 AT E173018 T1 ATE173018 T1 AT E173018T1 AT 90912715 T AT90912715 T AT 90912715T AT 90912715 T AT90912715 T AT 90912715T AT E173018 T1 ATE173018 T1 AT E173018T1
Authority
AT
Austria
Prior art keywords
fusion proteins
production
manner
fusion protein
oligonucleotide
Prior art date
Application number
AT90912715T
Other languages
English (en)
Inventor
Siegfried Stengelin
Wolfgang Ulmer
Paul Habermann
Eugen Uhlmann
Brian Seed
Original Assignee
Hoechst Ag
Gen Hospital Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag, Gen Hospital Corp filed Critical Hoechst Ag
Application granted granted Critical
Publication of ATE173018T1 publication Critical patent/ATE173018T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT90912715T 1989-08-29 1990-08-28 Herstellung von fusionsproteinen ATE173018T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US39987489A 1989-08-29 1989-08-29

Publications (1)

Publication Number Publication Date
ATE173018T1 true ATE173018T1 (de) 1998-11-15

Family

ID=23581315

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90912715T ATE173018T1 (de) 1989-08-29 1990-08-28 Herstellung von fusionsproteinen

Country Status (19)

Country Link
EP (1) EP0489780B1 (de)
JP (1) JP3043803B2 (de)
KR (1) KR0159786B1 (de)
AT (1) ATE173018T1 (de)
AU (1) AU638277B2 (de)
CA (1) CA2065146C (de)
CY (1) CY2168B1 (de)
DE (1) DE69032743T2 (de)
DK (1) DK0489780T3 (de)
ES (1) ES2124216T3 (de)
FI (1) FI113183B (de)
GR (1) GR1005153B (de)
HU (1) HU216069B (de)
IE (1) IE903120A1 (de)
IL (1) IL95495A (de)
NO (1) NO308667B1 (de)
PT (1) PT95111B (de)
WO (1) WO1991003550A1 (de)
ZA (1) ZA906839B (de)

Families Citing this family (37)

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US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
SG46612A1 (en) * 1992-12-02 1998-02-20 Hoechst Ag Process for obtaining proinsulin in processing correctly linked cystine bridges
DK0622376T3 (da) * 1993-04-27 2001-11-12 Hoechst Ag Amorfe monosfæriske former af insulinderivater
DE69435058T2 (de) * 1993-10-05 2008-04-30 Asahi Glass Co., Ltd. Multicloning-vektor, expressionsvektor und herstellung von fremdproteinen unter verwendung des expressionsvektors
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
US5683987A (en) * 1994-07-12 1997-11-04 The Board Of Regents Of The University Of Nebraska Therapeutic oligonucleotides targeting the human MDR1 and MRP genes
DE59711533D1 (de) 1996-07-26 2004-05-27 Aventis Pharma Gmbh Insulinderivate mit erhöhter Zinkbindung
DE19735711C2 (de) 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
DE19825447A1 (de) 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh Neue Insulinanaloga mit erhöhter Zinkbildung
DE19915938A1 (de) 1999-04-09 2000-10-19 Aventis Pharma Gmbh Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung
DE19930676B4 (de) 1999-07-02 2006-01-19 Sanofi-Aventis Deutschland Gmbh Verfahren zur Stabilisierung von Insulin, Insulinderivaten und/oder deren Vorläufer in komplexen Mischungen bei deren Lagerung in wäßrigen Lösungsmitteln
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE10235168A1 (de) 2002-08-01 2004-02-12 Aventis Pharma Deutschland Gmbh Verfahren zur Reinigung von Preproinsulin
EP1563075A4 (de) * 2002-11-12 2006-09-06 Ckd Bio Corp Humaninsulin exprimierende plasmide und verfahren zur herstellung von humaninsulin damit
DE102008003566A1 (de) 2008-01-09 2009-07-16 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
AU2009203809B2 (en) 2008-01-09 2013-07-25 Sanofi-Aventis Deutschland Gmbh Novel insulin derivatives having an extremely delayed time-action profile
DE102008025007A1 (de) 2008-05-24 2009-11-26 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
BRPI0907119A2 (pt) 2008-01-09 2015-07-14 Sanofi Aventis Deutschland Derivados de insulina tendo um perfil de ação de tempo extremamente retardado
DE102008025008A1 (de) 2008-05-24 2009-11-26 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
DE102008003568A1 (de) 2008-01-09 2009-07-16 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
PT3228320T (pt) 2008-10-17 2020-03-26 Sanofi Aventis Deutschland Combinação de uma insulina e de um agonista do glp-1
KR101853606B1 (ko) 2009-07-06 2018-05-03 사노피-아벤티스 도이칠란트 게엠베하 메티오닌을 함유하는 수성 인슐린 제제
KR101337322B1 (ko) 2009-11-13 2013-12-06 사노피-아벤티스 도이칠란트 게엠베하 Ｇｌｐ-1 효능제 및 메티오닌을 포함하는 약제학적 조성물
DK2498802T3 (en) 2009-11-13 2015-04-13 Sanofi Aventis Deutschland Pharmaceutical composition comprising a GLP-1 agonist, insulin and a methionine
ES2606554T3 (es) 2010-08-30 2017-03-24 Sanofi-Aventis Deutschland Gmbh Uso de AVE0010 para la fabricación de un medicamento para el tratamiento de la diabetes mellitus de tipo 2
PL2739646T3 (pl) 2011-05-10 2018-05-30 Stefan Hermann Kompleksowy układ buforowy do renaturacji ludzkiej proinsuliny lub pochodnych proinsuliny
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
PL2750699T3 (pl) 2011-08-29 2015-12-31 Sanofi Aventis Deutschland Kombinacja farmaceutyczna do stosowania w kontroli glikemii u pacjentów z cukrzycą typu 2
AR087744A1 (es) 2011-09-01 2014-04-16 Sanofi Aventis Deutschland Composicion farmaceutica para uso en el tratamiento de una enfermedad neurodegenerativa
JP5858945B2 (ja) 2012-03-15 2016-02-10 アークレイ株式会社 酵素を用いた測定方法
AU2015359376B2 (en) 2014-12-12 2021-09-09 Sanofi-Aventis Deutschland Gmbh Insulin glargine/lixisenatide fixed ratio formulation
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
US10660852B2 (en) * 2015-09-18 2020-05-26 Bionet Pharma Gmbh Expression and folding in the manufacturing process of CD-RAP by using a CD-RAP precursor protein
JP7460182B2 (ja) * 2019-02-19 2024-04-02 ザ、トラスティーズ オブ プリンストン ユニバーシティ フォールディングされたドメインおよびｒｎａ顆粒会合タンパク質ドメインの光制御オリゴマー化および相分離のためのシステムおよび方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366246A (en) * 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
US4431739A (en) * 1979-11-05 1984-02-14 Genentech, Inc. Transformant bacterial culture capable of expressing heterologous protein
US4769326A (en) * 1980-02-29 1988-09-06 The Regents Of The University Of California Expression linkers
US4652639A (en) * 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
EP0195680A3 (de) * 1985-03-21 1987-06-10 Immunex Corporation Proteinsynthese mit einem Identifizierungspeptid
DE3526995A1 (de) * 1985-07-27 1987-02-05 Hoechst Ag Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung

Also Published As

Publication number Publication date
IE903120A1 (en) 1991-03-13
GR900100635A (el) 1991-12-30
HK1012026A1 (en) 1999-07-23
EP0489780A1 (de) 1992-06-17
IL95495A (en) 1996-10-16
KR0159786B1 (ko) 1998-11-16
NO308667B1 (no) 2000-10-09
AU638277B2 (en) 1993-06-24
PT95111B (pt) 1998-10-30
JPH05501799A (ja) 1993-04-08
NO920774L (no) 1992-04-28
EP0489780B1 (de) 1998-11-04
FI920923A0 (fi) 1992-02-28
ES2124216T3 (es) 1999-02-01
FI113183B (fi) 2004-03-15
DE69032743T2 (de) 1999-06-17
CY2168B1 (en) 2002-08-23
WO1991003550A1 (en) 1991-03-21
AU6287290A (en) 1991-04-08
PT95111A (pt) 1991-05-22
IL95495A0 (en) 1991-06-30
GR1005153B (el) 2006-03-13
DE69032743D1 (de) 1998-12-10
HUT60327A (en) 1992-08-28
EP0489780A4 (en) 1992-08-12
DK0489780T3 (da) 1999-07-19
JP3043803B2 (ja) 2000-05-22
NO920774D0 (no) 1992-02-27
CA2065146A1 (en) 1991-03-01
ZA906839B (en) 1991-06-26
HU216069B (hu) 1999-04-28
HU9200674D0 (en) 1992-08-28
CA2065146C (en) 2002-07-23

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EEIH Change in the person of patent owner
EELA Cancelled due to lapse of time