ATE181736T1 - Verfahren zur reinigung von proteinen aus zellsystemen - Google Patents

Verfahren zur reinigung von proteinen aus zellsystemen

Info

Publication number: ATE181736T1
Authority: AT; Austria
Prior art keywords: pellet; cell systems; mixture; isoelectric point; proteins
Prior art date: 1992-05-22

Application number

AT93830191T

Other languages

English (en)

Inventor

Fabrizio Bonelli

Elvi Cecconato

Raffaele Calogero

Original Assignee

Wabco B V

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-05-22

Filing date

1993-05-03

Publication date

1999-07-15

1993-05-03 Application filed by Wabco B V filed Critical Wabco B V

1999-07-15 Application granted granted Critical

1999-07-15 Publication of ATE181736T1 publication Critical patent/ATE181736T1/de

Links

238000000034 method Methods 0.000 title abstract 2
102000004169 proteins and genes Human genes 0.000 title abstract 2
108090000623 proteins and genes Proteins 0.000 title abstract 2
239000008188 pellet Substances 0.000 abstract 3
239000000203 mixture Substances 0.000 abstract 2
102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
239000002253 acid Substances 0.000 abstract 1
239000003795 chemical substances by application Substances 0.000 abstract 1
238000004587 chromatography analysis Methods 0.000 abstract 1
239000003960 organic solvent Substances 0.000 abstract 1
230000001376 precipitating effect Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
- C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
- C12N2770/24261—Methods of inactivation or attenuation
- C12N2770/24264—Methods of inactivation or attenuation by serial passage

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biochemistry (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Genetics & Genomics (AREA)
Analytical Chemistry (AREA)
Virology (AREA)
Gastroenterology & Hepatology (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

AT93830191T 1992-05-22 1993-05-03 Verfahren zur reinigung von proteinen aus zellsystemen ATE181736T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
ITRM920385A IT1254370B (it)	1992-05-22	1992-05-22	Procedimento per la purificazione di proteine da sistemi cellulari.

Publications (1)

Publication Number	Publication Date
ATE181736T1 true ATE181736T1 (de)	1999-07-15

Family

ID=11401017

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT93830191T ATE181736T1 (de)	1992-05-22	1993-05-03	Verfahren zur reinigung von proteinen aus zellsystemen

Country Status (5)

Country	Link
EP (1)	EP0571337B1 (de)
AT (1)	ATE181736T1 (de)
DE (1)	DE69325479T2 (de)
ES (1)	ES2135455T3 (de)
IT (1)	IT1254370B (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
PL205149B1 (pl) *	2000-02-22	2010-03-31	Serono Lab	Sposób przygotowania rekombinowanej hCG

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH01300898A (ja) *	1988-05-28	1989-12-05	Ajinomoto Co Inc	生理活性蛋白質の精製法
ES2097806T3 (es) *	1990-03-30	1997-04-16	Shiseido Co Ltd	Procedimiento para purificar un polipeptido.

1992
- 1992-05-22 IT ITRM920385A patent/IT1254370B/it active
1993
- 1993-05-03 AT AT93830191T patent/ATE181736T1/de not_active IP Right Cessation
- 1993-05-03 ES ES93830191T patent/ES2135455T3/es not_active Expired - Lifetime
- 1993-05-03 EP EP93830191A patent/EP0571337B1/de not_active Expired - Lifetime
- 1993-05-03 DE DE69325479T patent/DE69325479T2/de not_active Expired - Fee Related

Also Published As

Publication number	Publication date
DE69325479T2 (de)	2000-01-05
ITRM920385A0 (it)	1992-05-22
EP0571337A2 (de)	1993-11-24
EP0571337A3 (en)	1994-07-06
IT1254370B (it)	1995-09-14
ITRM920385A1 (it)	1993-11-22
DE69325479D1 (de)	1999-08-05
EP0571337B1 (de)	1999-06-30
ES2135455T3 (es)	1999-11-01

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Legal Events

Date	Code	Title
2000-01-15	UEP	Publication of translation of european patent specification
2001-07-15	EEIH	Change in the person of patent owner
2002-02-15	REN	Ceased due to non-payment of the annual fee