ITRM920385A1 - Procedimento per la purificazione di proteine da sistemi cellulari. - Google Patents

Procedimento per la purificazione di proteine da sistemi cellulari.

Info

Publication number
ITRM920385A1
ITRM920385A1 IT000385A ITRM920385A ITRM920385A1 IT RM920385 A1 ITRM920385 A1 IT RM920385A1 IT 000385 A IT000385 A IT 000385A IT RM920385 A ITRM920385 A IT RM920385A IT RM920385 A1 ITRM920385 A1 IT RM920385A1
Authority
IT
Italy
Prior art keywords
proteins
pellet
cell systems
purification
procedure
Prior art date
Application number
IT000385A
Other languages
English (en)
Inventor
Fabrizio Bonelli
Raffaele Calogero
Elvi Cecconato
Original Assignee
Sorin Biomedica Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sorin Biomedica Spa filed Critical Sorin Biomedica Spa
Priority to ITRM920385A priority Critical patent/IT1254370B/it
Publication of ITRM920385A0 publication Critical patent/ITRM920385A0/it
Priority to ES93830191T priority patent/ES2135455T3/es
Priority to DE69325479T priority patent/DE69325479T2/de
Priority to AT93830191T priority patent/ATE181736T1/de
Priority to EP93830191A priority patent/EP0571337B1/en
Publication of ITRM920385A1 publication Critical patent/ITRM920385A1/it
Application granted granted Critical
Publication of IT1254370B publication Critical patent/IT1254370B/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24261—Methods of inactivation or attenuation
    • C12N2770/24264—Methods of inactivation or attenuation by serial passage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ITRM920385A 1992-05-22 1992-05-22 Procedimento per la purificazione di proteine da sistemi cellulari. IT1254370B (it)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ITRM920385A IT1254370B (it) 1992-05-22 1992-05-22 Procedimento per la purificazione di proteine da sistemi cellulari.
ES93830191T ES2135455T3 (es) 1992-05-22 1993-05-03 Procedimiento de purificacion de proteinas a partir de sistemas celulares.
DE69325479T DE69325479T2 (de) 1992-05-22 1993-05-03 Verfahren zur Reinigung von Proteinen aus Zellsystemen
AT93830191T ATE181736T1 (de) 1992-05-22 1993-05-03 Verfahren zur reinigung von proteinen aus zellsystemen
EP93830191A EP0571337B1 (en) 1992-05-22 1993-05-03 Process to purify proteins from cell systems

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITRM920385A IT1254370B (it) 1992-05-22 1992-05-22 Procedimento per la purificazione di proteine da sistemi cellulari.

Publications (3)

Publication Number Publication Date
ITRM920385A0 ITRM920385A0 (it) 1992-05-22
ITRM920385A1 true ITRM920385A1 (it) 1993-11-22
IT1254370B IT1254370B (it) 1995-09-14

Family

ID=11401017

Family Applications (1)

Application Number Title Priority Date Filing Date
ITRM920385A IT1254370B (it) 1992-05-22 1992-05-22 Procedimento per la purificazione di proteine da sistemi cellulari.

Country Status (5)

Country Link
EP (1) EP0571337B1 (it)
AT (1) ATE181736T1 (it)
DE (1) DE69325479T2 (it)
ES (1) ES2135455T3 (it)
IT (1) IT1254370B (it)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL205149B1 (pl) * 2000-02-22 2010-03-31 Serono Lab Sposób przygotowania rekombinowanej hCG

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01300898A (ja) * 1988-05-28 1989-12-05 Ajinomoto Co Inc 生理活性蛋白質の精製法
ES2097806T3 (es) * 1990-03-30 1997-04-16 Shiseido Co Ltd Procedimiento para purificar un polipeptido.

Also Published As

Publication number Publication date
DE69325479T2 (de) 2000-01-05
ITRM920385A0 (it) 1992-05-22
EP0571337A2 (en) 1993-11-24
EP0571337A3 (en) 1994-07-06
IT1254370B (it) 1995-09-14
DE69325479D1 (de) 1999-08-05
EP0571337B1 (en) 1999-06-30
ATE181736T1 (de) 1999-07-15
ES2135455T3 (es) 1999-11-01

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