ATE189477T1 - Mutierte penicillin-g-acylase gene - Google Patents

Mutierte penicillin-g-acylase gene

Info

Publication number
ATE189477T1
ATE189477T1 AT95929882T AT95929882T ATE189477T1 AT E189477 T1 ATE189477 T1 AT E189477T1 AT 95929882 T AT95929882 T AT 95929882T AT 95929882 T AT95929882 T AT 95929882T AT E189477 T1 ATE189477 T1 AT E189477T1
Authority
AT
Austria
Prior art keywords
penicillin
acylase
mutated penicillin
acylases
amino acid
Prior art date
Application number
AT95929882T
Other languages
English (en)
Inventor
Der Laan Jan Metske Van
Wilhelmus Johannes Quax
Adriana Marina Riemens
Original Assignee
Dsm Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nv filed Critical Dsm Nv
Application granted granted Critical
Publication of ATE189477T1 publication Critical patent/ATE189477T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C12P37/06—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by desacylation of the substituent in the 6 position

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT95929882T 1994-08-12 1995-08-14 Mutierte penicillin-g-acylase gene ATE189477T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP94202314 1994-08-12
PCT/EP1995/003249 WO1996005318A1 (en) 1994-08-12 1995-08-14 Mutated penicillin g acylase genes

Publications (1)

Publication Number Publication Date
ATE189477T1 true ATE189477T1 (de) 2000-02-15

Family

ID=8217100

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95929882T ATE189477T1 (de) 1994-08-12 1995-08-14 Mutierte penicillin-g-acylase gene

Country Status (10)

Country Link
US (2) US5891703A (de)
EP (1) EP0775210B1 (de)
JP (1) JP4147319B2 (de)
CN (1) CN1159444C (de)
AT (1) ATE189477T1 (de)
AU (1) AU3346295A (de)
DE (1) DE69514923T2 (de)
ES (1) ES2144139T3 (de)
MX (1) MX9701025A (de)
WO (1) WO1996005318A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
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AU3346295A (en) * 1994-08-12 1996-03-07 Gist-Brocades B.V. Mutated penicillin g acylase genes
WO1998020120A1 (en) * 1996-11-05 1998-05-14 Bristol-Myers Squibb Company Mutant penicillin g acylases
EP1315827A1 (de) * 2000-09-08 2003-06-04 Dsm N.V. Verfahren zur herstellung von angereicherten enantiomen aminen
EP1456370A2 (de) * 2001-12-27 2004-09-15 DSM IP Assets B.V. Verfahren zur herstellung von einem beta-laktam antibiotikum mittels einer mutierten penicillineacylase
JP4113427B2 (ja) * 2002-08-16 2008-07-09 富士フイルム株式会社 熱現像感光材料
DE10251747A1 (de) * 2002-11-05 2004-05-19 Tuhh-Technologie-Gmbh Gentechnisch veränderte Penicillinamidase und Verfahren zu ihrer Herstellung
WO2004111241A1 (en) * 2003-06-12 2004-12-23 Stichting Voor De Technische Wetenschappen Penicillin acylase
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
ITMI20040016A1 (it) * 2004-01-12 2004-04-12 Antibiotics S P A Cefalosporina c acilasi
CN101177688B (zh) * 2006-11-08 2010-12-01 中国科学院上海生命科学研究院 突变青霉素g酰化酶、其重组表达质粒及转化的工程菌株
CZ200782A3 (cs) * 2007-01-31 2009-05-27 Mikrobiologický ústav AV CR, v.v.i. Sekvence nukleotidu o velikosti 2646 bp, kódující penicilinacylázu, nové konstrukty rekombinantní nukleové kyseliny a rekombinantní mikroorganismy nesoucí tuto sekvenci
US8288131B2 (en) * 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides and uses thereof
EP2329013B1 (de) * 2008-08-27 2015-10-28 Codexis, Inc. Ketoreduktasepolypeptide zur herstellung von einem 3-aryl-3-hydroxypropanamin aus einem 3-aryl-3-ketopropanamin
WO2010025287A2 (en) * 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8273554B2 (en) 2008-08-29 2012-09-25 Codexis, Inc. Ketoreductase polypeptides for the stereoselective production of (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one
US8247192B2 (en) 2008-11-10 2012-08-21 Codexis, Inc. Penicillin-G acylases
CN102264904B (zh) * 2008-12-23 2013-10-23 中化帝斯曼制药有限公司荷兰公司 突变体青霉素g酰基转移酶
ES2575560T3 (es) 2009-08-19 2016-06-29 Codexis, Inc. Polipéptidos cetorreductasa para preparar fenilefrina
HUE026367T2 (en) 2010-05-04 2016-06-28 Codexis Inc Biocatalysts for ezetimibe synthesis
EP2723882B1 (de) 2011-06-23 2019-05-01 Centrient Pharmaceuticals Netherlands B.V. Verfahren zur herstellung von 3 '-thiosubstituierten cephalosporinen unter verwendung einer penicillin-g-acylase
WO2012175587A2 (en) 2011-06-23 2012-12-27 Dsm Sinochem Pharmaceuticals Netherlands B.V. Novel crystalline cefoperazone intermediate
WO2013057196A1 (en) 2011-10-20 2013-04-25 Dsm Sinochem Pharmaceuticals Netherlands B.V. Process for the preparation of cefamandole nafate
WO2013057197A1 (en) 2011-10-20 2013-04-25 Dsm Sinochem Pharmaceuticals Netherlands B.V. Process for the formylation of cefamandole
RU2537845C2 (ru) * 2012-04-25 2015-01-10 Федеральное государственное бюджетное образовательное учреждение высшего профессиональногообразования "Московский государственный университет имени М.В. Ломоносова " (МГУ) Способ синтеза пептидов, в том числе бета-лактамных антибиотиков, при использовании варианта пенициллинацилазы
CN103667418B (zh) * 2013-12-18 2015-03-25 华北制药河北华民药业有限责任公司 高活性β-内酰胺抗生素合成用酶的高通量筛选方法
JP6379531B2 (ja) * 2014-03-10 2018-08-29 東洋紡株式会社 新規β−アミノ酸の製造方法
CN107531762A (zh) 2015-05-07 2018-01-02 科德克希思公司 青霉素g酰化酶
WO2017186864A1 (en) 2016-04-27 2017-11-02 Sandoz Ag Enzymatic process for the production of beta-lactam antibiotics in the presence of particulate inoculum
CN117737045A (zh) * 2017-06-27 2024-03-22 科德克希思公司 青霉素g酰化酶
CN110343734B (zh) * 2019-06-14 2021-03-23 浙江工业大学 一种l-草铵膦化学酶法生产方法
CN111172142B (zh) * 2020-02-14 2021-09-28 上海陶宇晟生物技术有限责任公司 一种热稳定性高的头孢菌素c酰化酶突变体
CN120425012A (zh) 2024-10-29 2025-08-05 浙江师范大学行知学院 一种基于嗜柠檬酸克鲁维氏菌β亚基G385突变青霉素酰化酶催化一步法合成阿莫西林产物分离与回收的方法
CN120137953B (zh) * 2025-05-16 2025-08-15 成都大学 一种重组青霉素g酰化酶及其制备方法和应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6121097A (ja) * 1984-07-10 1986-01-29 Asahi Chem Ind Co Ltd ７−アミノセフアロスポラン酸及びその誘導体の製造法
US4981789A (en) * 1987-03-18 1991-01-01 Merck & Co., Inc. One-step enzymatic conversion of cephalosporin C and derivatives to 7-aminocephalosporanic acid and derivatives
EP0322032A3 (de) * 1987-12-21 1991-01-30 Merck & Co. Inc. Enzymatische Umwandlung von Cephalosporin C und Derivaten in 7-Aminocephalosporinsäure und Derivate in einem Schritt
ATE167520T1 (de) * 1990-04-18 1998-07-15 Gist Brocades Nv Mutierte beta-lactamacylasegene
PT97397B (pt) * 1990-04-18 1998-08-31 Gist Brocades Nv Processo para a preparacao de penicilina g acilase e de genes que a codificam
GB9019724D0 (en) * 1990-09-10 1990-10-24 Fujisawa Pharmaceutical Co Cephalosporin c acylase
GB9204439D0 (en) * 1992-02-27 1992-04-15 Fujisawa Pharmaceutical Co A new cephalosporin c acylase
TW400384B (en) * 1993-11-01 2000-08-01 Fujisawa Pharmaceutical Co A new cephalosporin C acylase
AU3346295A (en) * 1994-08-12 1996-03-07 Gist-Brocades B.V. Mutated penicillin g acylase genes
US5516679A (en) * 1994-12-23 1996-05-14 Bristol-Myers Squibb Company Penicillin V amidohydrolase gene from Fusarium oxysporum
WO1998020120A1 (en) * 1996-11-05 1998-05-14 Bristol-Myers Squibb Company Mutant penicillin g acylases

Also Published As

Publication number Publication date
CN1158638A (zh) 1997-09-03
EP0775210B1 (de) 2000-02-02
DE69514923T2 (de) 2000-11-02
AU3346295A (en) 1996-03-07
US6033823A (en) 2000-03-07
JPH10507072A (ja) 1998-07-14
US5891703A (en) 1999-04-06
DE69514923D1 (de) 2000-03-09
CN1159444C (zh) 2004-07-28
ES2144139T3 (es) 2000-06-01
JP4147319B2 (ja) 2008-09-10
MX9701025A (es) 1997-05-31
EP0775210A1 (de) 1997-05-28
WO1996005318A1 (en) 1996-02-22

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