ATE192486T1 - Chondroitinase, herstellungsverfahren und pharmazeutische zusammensetzung - Google Patents

Chondroitinase, herstellungsverfahren und pharmazeutische zusammensetzung

Info

Publication number: ATE192486T1
Authority: AT; Austria
Prior art keywords: chondroitinase; chondroitinase abc; crystallizable; sds; measurement
Prior art date: 1992-06-26

Application number

AT93304979T

Other languages

English (en)

Inventor

Nobukazu Hashimoto

Akio Hamai

Hideo Mochizuki

Shinichi Ishikawa

Misako Torikai

Isao Miyachi

Original Assignee

Seikagaku Kogyo Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-06-26

Filing date

1993-06-25

Publication date

2000-05-15

1992-10-26 Priority claimed from JP31098092A external-priority patent/JP3895782B2/ja

1993-06-25 Application filed by Seikagaku Kogyo Co Ltd filed Critical Seikagaku Kogyo Co Ltd

2000-05-15 Application granted granted Critical

2000-05-15 Publication of ATE192486T1 publication Critical patent/ATE192486T1/de

Links

238000000034 method Methods 0.000 title abstract 3
102000011413 Chondroitinases and Chondroitin Lyases Human genes 0.000 title abstract 2
108010023736 Chondroitinases and Chondroitin Lyases Proteins 0.000 title abstract 2
239000008194 pharmaceutical composition Substances 0.000 title 1
102000037716 Chondroitin-sulfate-ABC endolyases Human genes 0.000 abstract 3
108090000819 Chondroitin-sulfate-ABC endolyases Proteins 0.000 abstract 3
238000005259 measurement Methods 0.000 abstract 2
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 abstract 2
125000001433 C-terminal amino-acid group Chemical group 0.000 abstract 1
108010010803 Gelatin Proteins 0.000 abstract 1
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 abstract 1
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 1
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 abstract 1
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 abstract 1
102000007562 Serum Albumin Human genes 0.000 abstract 1
108010071390 Serum Albumin Proteins 0.000 abstract 1
235000004279 alanine Nutrition 0.000 abstract 1
210000004027 cell Anatomy 0.000 abstract 1
238000010828 elution Methods 0.000 abstract 1
238000005227 gel permeation chromatography Methods 0.000 abstract 1
239000008273 gelatin Substances 0.000 abstract 1
229920000159 gelatin Polymers 0.000 abstract 1
235000019322 gelatine Nutrition 0.000 abstract 1
235000011852 gelatine desserts Nutrition 0.000 abstract 1
244000005700 microbiome Species 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
239000002736 nonionic surfactant Substances 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
238000000746 purification Methods 0.000 abstract 1
239000012607 strong cation exchange resin Substances 0.000 abstract 1
239000004094 surface-active agent Substances 0.000 abstract 1
239000012608 weak cation exchange resin Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/2063—Proteins, e.g. gelatin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
- C12Y402/02004—Chondroitin ABC lyase (4.2.2.4), i.e. chondroitinase
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03C—PHOTOSENSITIVE MATERIALS FOR PHOTOGRAPHIC PURPOSES; PHOTOGRAPHIC PROCESSES, e.g. CINE, X-RAY, COLOUR, STEREO-PHOTOGRAPHIC PROCESSES; AUXILIARY PROCESSES IN PHOTOGRAPHY
- G03C1/00—Photosensitive materials
- G03C1/005—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein
- G03C1/04—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein with macromolecular additives; with layer-forming substances
- G03C1/047—Proteins, e.g. gelatine derivatives; Hydrolysis or extraction products of proteins
- G03C2001/0471—Isoelectric point of gelatine
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/873—Proteus

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Animal Behavior & Ethology (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Pharmacology & Pharmacy (AREA)
Epidemiology (AREA)
Analytical Chemistry (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Enzymes And Modification Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Medicinal Preparation (AREA)

AT93304979T 1992-06-26 1993-06-25 Chondroitinase, herstellungsverfahren und pharmazeutische zusammensetzung ATE192486T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
JP19288292		1992-06-26
JP31098092A JP3895782B2 (ja)	1992-10-26	1992-10-26	コンドロイチナーゼ組成物およびそれを含有する注射用製剤

Publications (1)

Publication Number	Publication Date
ATE192486T1 true ATE192486T1 (de)	2000-05-15

Family

ID=26507572

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT93304979T ATE192486T1 (de)	1992-06-26	1993-06-25	Chondroitinase, herstellungsverfahren und pharmazeutische zusammensetzung

Country Status (10)

Country	Link
US (3)	US5496718A (de)
EP (1)	EP0576294B1 (de)
KR (1)	KR100280779B1 (de)
AT (1)	ATE192486T1 (de)
CA (1)	CA2099138C (de)
DE (1)	DE69328519T2 (de)
DK (1)	DK0576294T3 (de)
ES (1)	ES2146600T3 (de)
RU (1)	RU2144833C1 (de)
SG (1)	SG50677A1 (de)

Families Citing this family (47)

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US5496718A (en) *	1992-06-26	1996-03-05	Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)	Chondroitinase ABC isolated from proteus vulgaris ATCC 6896
JP3419811B2 (ja) *	1993-02-24	2003-06-23	マルハ株式会社	コンドロイチナーゼ遺伝子
US7008783B1 (en)	1992-09-22	2006-03-07	Maruha Corporation	Gene encoding chondroitinase ABC and uses therefor
AU697156B2 (en) *	1993-04-23	1998-10-01	American Cyanamid Company	Cloning and expression of the chondroitinase I and II genes from (p. vulgaris)
AU1176595A (en) *	1993-11-12	1995-05-29	International Technology Management Associates, Ltd.	Methods of repairing connective tissues
US5525500A (en) *	1994-04-22	1996-06-11	American Cyanamid Company	Chromatographic process for the copurification of chondroitinase I and II proteins from Proteus vulgaris
US5498536A (en) *	1994-04-22	1996-03-12	American Cyanamid Company	Chondroitinase II from Proteus vulgaris
EP0756636A4 (de) *	1994-04-22	1997-12-17	American Cyanamid Co	Chondroitinase i und ii, herstellungsverfahren und ihre verwendung
US5888798A (en) *	1995-06-07	1999-03-30	American Cyanamid Company	Chondroitinase I and chondroitinase II producing mutants of P. vulgaris
EP0747469A1 (de)	1995-06-08	1996-12-11	The Procter & Gamble Company	Chondroitinase enthaltende Reinigungszusammensetzungen
DE69829605T2 (de) *	1997-05-02	2006-02-09	Seikagaku Corp.	Chondroitinase enthaltende Zusammensetzungen
JPH1149698A (ja)	1997-07-31	1999-02-23	Santen Pharmaceut Co Ltd	安定性を向上させたラクトフェリンの水性製剤
ES2185117T3 (es) *	1997-08-22	2003-04-16	Seikagaku Kogyo Co Ltd	Agente terapeutico para disco intervertebral herniado.
TWI245645B (en)	1999-09-08	2005-12-21	Chugai Pharmaceutical Co Ltd	Protein solution formulations and stabilization methods thereof
JP4252892B2 (ja)	2001-06-01	2009-04-08	三菱製紙株式会社	全熱交換素子用紙
CA2455827C (en)	2001-08-13	2015-06-09	University Of Florida Research Foundation, Inc.	Materials and methods for nerve grafting comprising degrading chondroitin sulfate proteoglycan
GB0205022D0 (en)	2002-03-04	2002-04-17	Univ Cambridge Tech	Materials and methods for the treatment of cns damage
EP1575548B1 (de) *	2002-05-04	2011-03-09	Acorda Therapeutics, Inc.	Zusammensetzungen und verfahren zur förderung des neuronalen wachstums
WO2003102160A2 (en) *	2002-06-03	2003-12-11	Massachusetts Institute Of Technology	Rationally designed polysaccharide lyases derived from chondroitinase b
DE60337009D1 (de) *	2002-08-15	2011-06-16	Acorda Therapeutics Inc	Chimärisches protein
US7544499B2 (en) *	2002-12-16	2009-06-09	Halozyme, Inc.	Human chondroitinase glycoprotein (CHASEGP), process for preparing the same, and pharmaceutical compositions comprising thereof
EP2330213A1 (de)	2003-03-05	2011-06-08	Halozyme, Inc.	Lösliches Hyaluronidaseglykoprotein (sHASEGP), Verfahren zu dessen Herstellung, Verwendungen und pharmazeutische Zusammensetzunge, die dieses umfassen
US20090123367A1 (en) *	2003-03-05	2009-05-14	Delfmems	Soluble Glycosaminoglycanases and Methods of Preparing and Using Soluble Glycosaminoglycanases
US7871607B2 (en)	2003-03-05	2011-01-18	Halozyme, Inc.	Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
US20060104968A1 (en) *	2003-03-05	2006-05-18	Halozyme, Inc.	Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminogly ycanases
EP3211084B1 (de)	2003-05-16	2019-03-27	Acorda Therapeutics, Inc.	Proteoglycan-abbauende mutanten zur behandlung des zns
MXPA05012305A (es) *	2003-05-16	2006-04-18	Acorda Therapeutics Inc	Composiciones y metodos para el tratamiento de lesiones del snc.
US7959914B2 (en)	2003-05-16	2011-06-14	Acorda Therapeutics, Inc.	Methods of reducing extravasation of inflammatory cells
RU2259350C2 (ru) *	2003-07-14	2005-08-27	Открытое акционерное общество "Пигмент"	Способ получения анилина и/или n-метиланилина и катализатор для него
GB0403247D0 (en)	2004-02-13	2004-03-17	Tillotts Pharma Ag	A pharmaceutical composition
CA2558984A1 (en) *	2004-03-10	2005-09-22	Massachusetts Institute Of Technology	Recombinant chondroitinase abc i and uses thereof
AU2005244933B2 (en) *	2004-05-18	2011-08-04	Acorda Therapeutics, Inc.	Methods of purifying chondroitinase and stable formulations thereof
WO2005122734A2 (en) *	2004-06-14	2005-12-29	The Research Foundation Of State University Of New York	Nanosphere/microsphere delivery system for the treatment of spinal cord injury
EP1848812A4 (de) *	2004-10-01	2011-11-09	Life Technologies Corp	Zufuhrpuffer, systeme und verfahren zur in-vitro-synthese von biomolekülen
RU2275190C1 (ru) *	2005-04-06	2006-04-27	Открытое акционерное общество "Нижегородский химико-фармацевтический завод"	Средство для лечения болезней суставов
JP5189985B2 (ja)	2005-09-26	2013-04-24	アコーダセラピューティクス、インク．	コンドロイチナーゼａｂｃｉ変異体を用いた組成物およびその使用方法
US8003369B2 (en)	2005-11-29	2011-08-23	Sanyo Chemical Industries, Ltd.	Bacteriolytic agent
AU2007307736C1 (en)	2006-10-10	2014-04-10	Acorda Therapeutics, Inc.	Compositions and methods of using chondroitinase ABCI mutants
JP5341756B2 (ja)	2007-06-29	2013-11-13	国立大学法人名古屋大学	神経障害に基づく機能不全の改善剤およびＲｈｏキナーゼ活性化抑制剤
US20090286289A1 (en) *	2008-03-10	2009-11-19	Pang Danny Z	Production of Hyaluronate Unsaturated Disaccharides and its Application
DK2653168T3 (da) *	2010-12-13	2015-04-20	Seikagaku Kogyo Co Ltd	Chondroitinase-abc til behandling af diskusprolaps
JP6580581B2 (ja)	2014-09-27	2019-09-25	大日本住友製薬株式会社	注射用医薬組成物
US9796970B1 (en)	2017-04-24	2017-10-24	Advantek Serum Laboratories Ltd.	Production of high purity chondroitinase ABC
EP4512425A3 (de)	2018-02-28	2025-05-21	Seikagaku Corporation	Verpackung und verfahren zu deren herstellung
TW202513090A (zh)	2018-02-28	2025-04-01	日商生化學工業股份有限公司	醫藥組成物、包裝體及其製造方法
EP4175969A4 (de) *	2020-07-01	2024-07-24	Medimmune, LLC	Reinigungsmittel und verfahren zur reinigung eines biotherapeutikums
CN114409731B (zh) *	2022-01-05	2023-08-11	浙江省农业科学院	同时具有激活乙醇脱氢酶和乙醛脱氢酶活性的两种多肽

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US3549500A (en) *	1967-07-22	1970-12-22	Seikagaku Kogyo Co Ltd	Novel chondroitinase and method of its recovery
JPS4832351B1 (de) *	1968-06-12	1973-10-05
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JPS6031479B2 (ja) *	1978-02-10	1985-07-22	生化学工業株式会社	純コンドロイチナ−ゼｃの製造法
JPS6031478B2 (ja) *	1978-02-10	1985-07-22	生化学工業株式会社	純コンドロイチナ−ゼｂの製造法
EP0009781B2 (de) *	1978-09-29	1986-04-23	Mitsubishi Kasei Corporation	Verfahren zur Reinigung langkettiger Acyl-Coenzym-A-Synthetase und das so gereinigte Enzym, Acyl-CoA-Synthetase
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JPS62122588A (ja) *	1985-11-22	1987-06-03	Taiyo Fishery Co Ltd	純コンドロイチナ−ゼの製造法
JP2726275B2 (ja) *	1988-08-24	1998-03-11	生化学工業株式会社	グリコサミノグリカン分解酵素の精製法
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JP3140797B2 (ja) *	1991-04-26	2001-03-05	生化学工業株式会社	安定化コンドロイチナーゼａｂｃ、その保存方法及び治療剤
US5496718A (en) *	1992-06-26	1996-03-05	Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)	Chondroitinase ABC isolated from proteus vulgaris ATCC 6896

1993
- 1993-06-23 US US08/082,853 patent/US5496718A/en not_active Expired - Lifetime
- 1993-06-25 ES ES93304979T patent/ES2146600T3/es not_active Expired - Lifetime
- 1993-06-25 CA CA002099138A patent/CA2099138C/en not_active Expired - Lifetime
- 1993-06-25 RU RU93046317A patent/RU2144833C1/ru active
- 1993-06-25 AT AT93304979T patent/ATE192486T1/de active
- 1993-06-25 SG SG1996008458A patent/SG50677A1/en unknown
- 1993-06-25 DE DE69328519T patent/DE69328519T2/de not_active Expired - Lifetime
- 1993-06-25 DK DK93304979T patent/DK0576294T3/da active
- 1993-06-25 KR KR1019930011667A patent/KR100280779B1/ko not_active Expired - Lifetime
- 1993-06-25 EP EP93304979A patent/EP0576294B1/de not_active Expired - Lifetime
1995
- 1995-05-18 US US08/443,675 patent/US5763205A/en not_active Expired - Lifetime
1997
- 1997-07-07 US US08/888,530 patent/US6184023B1/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
KR940005793A (ko)	1994-03-22
US5496718A (en)	1996-03-05
DK0576294T3 (da)	2000-10-09
CA2099138C (en)	1998-09-22
US5763205A (en)	1998-06-09
AU669528B2 (en)	1996-06-13
DE69328519T2 (de)	2000-09-14
EP0576294A3 (en)	1994-08-17
RU2144833C1 (ru)	2000-01-27
EP0576294B1 (de)	2000-05-03
CA2099138A1 (en)	1993-12-27
ES2146600T3 (es)	2000-08-16
DE69328519D1 (de)	2000-06-08
EP0576294A2 (de)	1993-12-29
AU4146493A (en)	1994-01-06
US6184023B1 (en)	2001-02-06
SG50677A1 (en)	1998-07-20
KR100280779B1 (ko)	2001-02-01

Legal Events

Date	Code	Title	Description
2000-10-15	UEP	Publication of translation of european patent specification

Publication	Publication Date	Title
DE69328519D1 (de)	2000-06-08	Chondroitinase, Herstellungsverfahren und pharmazeutische Zusammensetzung
RU93046317A (ru)	1996-05-20	Хиндроитиназа, способ ее получения и содержание ее фармацевтические композиции
Ruoslahti et al.	1974	Novel human serum protein from fibroblast plasma membrane
Feinstein et al.	1975	A rapid method of purification of human granulocyte cationic neutral proteases: purification and further characterization of human granulocyte elastase
Dimuzio et al.	1978	Phosphophoryns—major noncollagenous proteins of rat incisor dentin
Dayton et al.	1976	A Ca2+ ion-activated protease possibly involved in myofibrillar protein turnover. Purification from porcine muscle
Bennick et al.	1971	Purification and partial characterization of four proteins from human parotid saliva
DK0447585T3 (da)	1995-05-29	Fremgangsmåde til fremstilling af et intravenøst tolerant immunglobulin-G-præparat
ATE173478T1 (de)	1998-12-15	Varianten des pankreatischen rinder- trypsininhibitors, deren herstellung und verwendung
DK426386A (da)	1987-03-07	Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer
IE860305L (en)	1986-08-01	Method for purifying antihemophilic factor
Joubert	1973	Snake venom toxins: The amino acid sequences of two toxins from Ophiophagus hannah (King Cobra) venom
ATE68826T1 (de)	1991-11-15	Verfahren zur herstellung von reifem, menschlichem serum-albumin.
ES2058538T3 (es)	1994-11-01	Compuestos de proteina a purificados y procedimiento para su preparacion.
KR880701246A (ko)	1988-07-26	재조합 페포 계면활성제 단백질
JPS63503352A (ja)	1988-12-08	タンパク質の精製
ES2068827T3 (es)	1995-05-01	Produccion de proteinas en forma activa.
ATE121754T1 (de)	1995-05-15	Vakzine gegen bordetella.
CA2253246A1 (en)	1997-11-06	Purified multimerase
DE3584775D1 (de)	1992-01-09	Zusammensetzung zur behandlung von an haemophilia-a-inhibitor leidenden kranken.
DE58907555D1 (de)	1994-06-01	Verfahren zur Anreicherung der Blutgerinnungsfaktoren II, VII, IX und X.
Takahashi et al.	1982	Purification and characterization of anticomplement factor (cobra venom factor) from the Naja naja atra venom
Wier et al.	1986	Aproliferin--a human plasma protein that induces the irreversible loss of proliferative potential associated with terminal differentiation
YUMOTO et al.	1984	Comparison of tryptic peptides from the heavy and light subunits of calpain I and calpain II by high performance liquid chromatography
Roberts et al.	1982	A structural comparison of the subunits of the Dolichos biflorus seed lectin by peptide mapping and carboxyl terminal amino acid analysis