ATE196652T1 - In kunstlisches rekombinantes substrat ragg-1 und nativem aggrecan zum studium der proteolytischen wirkung von 'aggrecanase' in zellkultursystemen - Google Patents
In kunstlisches rekombinantes substrat ragg-1 und nativem aggrecan zum studium der proteolytischen wirkung von 'aggrecanase' in zellkultursystemenInfo
- Publication number
- ATE196652T1 ATE196652T1 AT96120949T AT96120949T ATE196652T1 AT E196652 T1 ATE196652 T1 AT E196652T1 AT 96120949 T AT96120949 T AT 96120949T AT 96120949 T AT96120949 T AT 96120949T AT E196652 T1 ATE196652 T1 AT E196652T1
- Authority
- AT
- Austria
- Prior art keywords
- aggrecanase
- recombinant substrate
- study
- ragg
- human igg1
- Prior art date
Links
- 108010003059 aggrecanase Proteins 0.000 title abstract 5
- 239000000758 substrate Substances 0.000 title abstract 5
- 102000016284 Aggrecans Human genes 0.000 title 1
- 108010067219 Aggrecans Proteins 0.000 title 1
- 238000004113 cell culture Methods 0.000 title 1
- 230000002797 proteolythic effect Effects 0.000 title 1
- 229940123879 Aggrecanase inhibitor Drugs 0.000 abstract 1
- 101000999998 Homo sapiens Aggrecan core protein Proteins 0.000 abstract 1
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 102000043967 human ACAN Human genes 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4725—Proteoglycans, e.g. aggreccan
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Food Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96100682 | 1996-01-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE196652T1 true ATE196652T1 (de) | 2000-10-15 |
Family
ID=8222415
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96120949T ATE196652T1 (de) | 1996-01-18 | 1996-12-27 | In kunstlisches rekombinantes substrat ragg-1 und nativem aggrecan zum studium der proteolytischen wirkung von 'aggrecanase' in zellkultursystemen |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US5872209A (de) |
| EP (1) | EP0785274B1 (de) |
| JP (1) | JP3973253B2 (de) |
| AT (1) | ATE196652T1 (de) |
| AU (1) | AU713828B2 (de) |
| CA (1) | CA2195385C (de) |
| DE (1) | DE69610483T2 (de) |
| DK (1) | DK0785274T3 (de) |
| ES (1) | ES2150064T3 (de) |
| GR (1) | GR3034649T3 (de) |
| PT (1) | PT785274E (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69610483T2 (de) * | 1996-01-18 | 2001-06-13 | Aventis Pharma Deutschland Gmbh | In kunstlisches Rekombinantes Substrat rAGG-1 und nativem Aggrecan zum Studium der proteolytischen Wirkung von 'Aggrecanase' in Zellkultursystemen |
| US6451575B1 (en) | 1997-07-25 | 2002-09-17 | Bristol-Myers Squibb Pharma Company | Aggrecan degrading metallo proteases |
| WO2000005256A1 (en) * | 1998-07-24 | 2000-02-03 | Du Pont Pharmaceuticals Company | Assays and peptide substrate for determining aggrecan degrading metallo protease activity |
| US6649377B1 (en) | 1999-05-10 | 2003-11-18 | Syntex (U.S.A.) Llc | Human aggrecanase and nucleic acid compositions encoding the same |
| EP1435980A4 (de) | 2001-09-15 | 2006-06-07 | Univ California | Stratifiziertes knorpelgewebe und verfahren zu seiner hestellung |
| US20030165473A1 (en) * | 2001-11-09 | 2003-09-04 | Rush-Presbyterian-St. Luke's Medical Center | Engineered intervertebral disc tissue |
| DE102004029470A1 (de) * | 2004-06-18 | 2006-01-05 | InViTek Gesellschaft für Biotechnik & Biodesign mbH | Verfahren zur Bestimmung von Aggrekanase-Aktivitäten |
| DE102005005972A1 (de) | 2005-02-09 | 2006-08-17 | Sanofi-Aventis Deutschland Gmbh | Verwendung von Thiopeptoliden zur Aktivitätsbestimmung von ADAM-T8 Proteasen |
| WO2010013138A2 (en) * | 2008-07-28 | 2010-02-04 | Mnd Diagnostics, Ltd. | Methods and compositions for detection of a pathogen, disease, medical condition, or biomarker thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5427954A (en) * | 1992-04-29 | 1995-06-27 | Shriner's Hospitals For Crippled Children | Compositions and methods for detection and treatment of human osteoarthritis |
| GB9310978D0 (en) * | 1993-05-27 | 1993-07-14 | Zeneca Ltd | Compounds |
| US5851523A (en) * | 1994-03-24 | 1998-12-22 | Ludwig Institute For Cancer Research. | Isolated, peptides derived from MAGE tumor rejection antigen precursors which complex with HLA-A2 molecules and uses thereof |
| SE9500023L (sv) * | 1994-05-17 | 1995-11-18 | Beki Ab | Sätt att detektera cancer |
| DE69610483T2 (de) * | 1996-01-18 | 2001-06-13 | Aventis Pharma Deutschland Gmbh | In kunstlisches Rekombinantes Substrat rAGG-1 und nativem Aggrecan zum Studium der proteolytischen Wirkung von 'Aggrecanase' in Zellkultursystemen |
-
1996
- 1996-12-27 DE DE69610483T patent/DE69610483T2/de not_active Expired - Lifetime
- 1996-12-27 EP EP96120949A patent/EP0785274B1/de not_active Expired - Lifetime
- 1996-12-27 PT PT96120949T patent/PT785274E/pt unknown
- 1996-12-27 AT AT96120949T patent/ATE196652T1/de active
- 1996-12-27 ES ES96120949T patent/ES2150064T3/es not_active Expired - Lifetime
- 1996-12-27 DK DK96120949T patent/DK0785274T3/da active
-
1997
- 1997-01-16 AU AU10199/97A patent/AU713828B2/en not_active Ceased
- 1997-01-17 CA CA2195385A patent/CA2195385C/en not_active Expired - Fee Related
- 1997-01-17 US US08/784,512 patent/US5872209A/en not_active Expired - Lifetime
- 1997-01-20 JP JP02097097A patent/JP3973253B2/ja not_active Expired - Fee Related
-
1998
- 1998-10-21 US US09/176,228 patent/US6180334B1/en not_active Expired - Lifetime
-
2000
- 2000-10-20 GR GR20000402336T patent/GR3034649T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| GR3034649T3 (en) | 2001-01-31 |
| JPH09191889A (ja) | 1997-07-29 |
| JP3973253B2 (ja) | 2007-09-12 |
| DK0785274T3 (da) | 2000-12-27 |
| ES2150064T3 (es) | 2000-11-16 |
| DE69610483T2 (de) | 2001-06-13 |
| CA2195385C (en) | 2010-07-27 |
| AU713828B2 (en) | 1999-12-09 |
| PT785274E (pt) | 2001-01-31 |
| US5872209A (en) | 1999-02-16 |
| CA2195385A1 (en) | 1997-07-19 |
| US6180334B1 (en) | 2001-01-30 |
| EP0785274B1 (de) | 2000-09-27 |
| DE69610483D1 (de) | 2000-11-02 |
| AU1019997A (en) | 1997-07-24 |
| EP0785274A1 (de) | 1997-07-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |