ATE196652T1 - In kunstlisches rekombinantes substrat ragg-1 und nativem aggrecan zum studium der proteolytischen wirkung von 'aggrecanase' in zellkultursystemen - Google Patents

In kunstlisches rekombinantes substrat ragg-1 und nativem aggrecan zum studium der proteolytischen wirkung von 'aggrecanase' in zellkultursystemen

Info

Publication number
ATE196652T1
ATE196652T1 AT96120949T AT96120949T ATE196652T1 AT E196652 T1 ATE196652 T1 AT E196652T1 AT 96120949 T AT96120949 T AT 96120949T AT 96120949 T AT96120949 T AT 96120949T AT E196652 T1 ATE196652 T1 AT E196652T1
Authority
AT
Austria
Prior art keywords
aggrecanase
recombinant substrate
study
ragg
human igg1
Prior art date
Application number
AT96120949T
Other languages
English (en)
Inventor
Eckart Bartnik
Bernd Eidenmueller
Frank Buettner
Bruce Caterson
Clare Hughes
Original Assignee
Aventis Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Gmbh filed Critical Aventis Pharma Gmbh
Application granted granted Critical
Publication of ATE196652T1 publication Critical patent/ATE196652T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/02Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4725Proteoglycans, e.g. aggreccan
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AT96120949T 1996-01-18 1996-12-27 In kunstlisches rekombinantes substrat ragg-1 und nativem aggrecan zum studium der proteolytischen wirkung von 'aggrecanase' in zellkultursystemen ATE196652T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP96100682 1996-01-18

Publications (1)

Publication Number Publication Date
ATE196652T1 true ATE196652T1 (de) 2000-10-15

Family

ID=8222415

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96120949T ATE196652T1 (de) 1996-01-18 1996-12-27 In kunstlisches rekombinantes substrat ragg-1 und nativem aggrecan zum studium der proteolytischen wirkung von 'aggrecanase' in zellkultursystemen

Country Status (11)

Country Link
US (2) US5872209A (de)
EP (1) EP0785274B1 (de)
JP (1) JP3973253B2 (de)
AT (1) ATE196652T1 (de)
AU (1) AU713828B2 (de)
CA (1) CA2195385C (de)
DE (1) DE69610483T2 (de)
DK (1) DK0785274T3 (de)
ES (1) ES2150064T3 (de)
GR (1) GR3034649T3 (de)
PT (1) PT785274E (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69610483T2 (de) * 1996-01-18 2001-06-13 Aventis Pharma Deutschland Gmbh In kunstlisches Rekombinantes Substrat rAGG-1 und nativem Aggrecan zum Studium der proteolytischen Wirkung von 'Aggrecanase' in Zellkultursystemen
US6451575B1 (en) 1997-07-25 2002-09-17 Bristol-Myers Squibb Pharma Company Aggrecan degrading metallo proteases
WO2000005256A1 (en) * 1998-07-24 2000-02-03 Du Pont Pharmaceuticals Company Assays and peptide substrate for determining aggrecan degrading metallo protease activity
US6649377B1 (en) 1999-05-10 2003-11-18 Syntex (U.S.A.) Llc Human aggrecanase and nucleic acid compositions encoding the same
EP1435980A4 (de) 2001-09-15 2006-06-07 Univ California Stratifiziertes knorpelgewebe und verfahren zu seiner hestellung
US20030165473A1 (en) * 2001-11-09 2003-09-04 Rush-Presbyterian-St. Luke's Medical Center Engineered intervertebral disc tissue
DE102004029470A1 (de) * 2004-06-18 2006-01-05 InViTek Gesellschaft für Biotechnik & Biodesign mbH Verfahren zur Bestimmung von Aggrekanase-Aktivitäten
DE102005005972A1 (de) 2005-02-09 2006-08-17 Sanofi-Aventis Deutschland Gmbh Verwendung von Thiopeptoliden zur Aktivitätsbestimmung von ADAM-T8 Proteasen
WO2010013138A2 (en) * 2008-07-28 2010-02-04 Mnd Diagnostics, Ltd. Methods and compositions for detection of a pathogen, disease, medical condition, or biomarker thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427954A (en) * 1992-04-29 1995-06-27 Shriner's Hospitals For Crippled Children Compositions and methods for detection and treatment of human osteoarthritis
GB9310978D0 (en) * 1993-05-27 1993-07-14 Zeneca Ltd Compounds
US5851523A (en) * 1994-03-24 1998-12-22 Ludwig Institute For Cancer Research. Isolated, peptides derived from MAGE tumor rejection antigen precursors which complex with HLA-A2 molecules and uses thereof
SE9500023L (sv) * 1994-05-17 1995-11-18 Beki Ab Sätt att detektera cancer
DE69610483T2 (de) * 1996-01-18 2001-06-13 Aventis Pharma Deutschland Gmbh In kunstlisches Rekombinantes Substrat rAGG-1 und nativem Aggrecan zum Studium der proteolytischen Wirkung von 'Aggrecanase' in Zellkultursystemen

Also Published As

Publication number Publication date
GR3034649T3 (en) 2001-01-31
JPH09191889A (ja) 1997-07-29
JP3973253B2 (ja) 2007-09-12
DK0785274T3 (da) 2000-12-27
ES2150064T3 (es) 2000-11-16
DE69610483T2 (de) 2001-06-13
CA2195385C (en) 2010-07-27
AU713828B2 (en) 1999-12-09
PT785274E (pt) 2001-01-31
US5872209A (en) 1999-02-16
CA2195385A1 (en) 1997-07-19
US6180334B1 (en) 2001-01-30
EP0785274B1 (de) 2000-09-27
DE69610483D1 (de) 2000-11-02
AU1019997A (en) 1997-07-24
EP0785274A1 (de) 1997-07-23

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