ATE197608T1 - Signalsequenzen für die secretion heterologer proteine von hefe - Google Patents

Signalsequenzen für die secretion heterologer proteine von hefe

Info

Publication number
ATE197608T1
ATE197608T1 AT94119437T AT94119437T ATE197608T1 AT E197608 T1 ATE197608 T1 AT E197608T1 AT 94119437 T AT94119437 T AT 94119437T AT 94119437 T AT94119437 T AT 94119437T AT E197608 T1 ATE197608 T1 AT E197608T1
Authority
AT
Austria
Prior art keywords
secretion
proteins
heterologue
yeast
signal sequences
Prior art date
Application number
AT94119437T
Other languages
English (en)
Inventor
Bong Hyun Chung
Soo Wan Nam
Byung Moon Kim
Sun Ah Yang
Young Hoon Park
Original Assignee
Korea Inst Sci & Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Inst Sci & Tech filed Critical Korea Inst Sci & Tech
Application granted granted Critical
Publication of ATE197608T1 publication Critical patent/ATE197608T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01007—Inulinase (3.2.1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT94119437T 1993-12-10 1994-12-08 Signalsequenzen für die secretion heterologer proteine von hefe ATE197608T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR930027269 1993-12-10

Publications (1)

Publication Number Publication Date
ATE197608T1 true ATE197608T1 (de) 2000-12-15

Family

ID=19370595

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94119437T ATE197608T1 (de) 1993-12-10 1994-12-08 Signalsequenzen für die secretion heterologer proteine von hefe

Country Status (5)

Country Link
US (1) US5712113A (de)
EP (1) EP0662515B1 (de)
JP (1) JP2799348B2 (de)
AT (1) ATE197608T1 (de)
DE (1) DE69426289T2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3730256B2 (ja) * 1995-02-03 2005-12-21 旭硝子株式会社 分泌シグナル遺伝子およびそれを有する発現ベクター
CN1646085A (zh) * 2002-04-22 2005-07-27 宝洁公司 包含阳离子瓜耳胶衍生物的洗发剂
JP2007525188A (ja) 2003-05-16 2007-09-06 インターミューン インコーポレイテッド 合成ケモカイン受容体リガンドおよびその使用方法
DE10342794A1 (de) * 2003-09-16 2005-04-21 Basf Ag Sekretion von Proteinen aus Hefen
JP5148879B2 (ja) 2004-01-17 2013-02-20 コリア リサーチ インスティチュート オブ バイオサイエンス アンド バイオテクノロジー 難発現性タンパク質の分泌のためのタンパク質融合因子（ｔｆｐ）を明らかにする方法、タンパク質融合因子（ｔｆｐ）ライブラリーを製造する方法、及び難発現性タンパク質の組み換え的生産方法
US7597884B2 (en) 2004-08-09 2009-10-06 Alios Biopharma, Inc. Hyperglycosylated polypeptide variants and methods of use
CA2576030A1 (en) * 2004-08-09 2006-02-23 Alios Biopharma Inc. Synthetic hyperglycosylated, protease-resistant polypeptide variants, oral formulations and methods of using the same
US7892788B2 (en) 2005-02-07 2011-02-22 Basf Se Hydrophobin fusion products, production and use thereof
BRPI0607594A2 (pt) 2005-03-31 2010-04-06 Basf Ag compósito multi-camadas ou substrato revestido, processo para a preparação dos mesmos, e, uso de hidrofobinas
MX2007011955A (es) 2005-04-01 2007-12-05 Basf Ag Uso de hidrofobina como estabilizador de fases.
MX270311B (es) 2005-04-01 2009-09-23 Basf Ag Fluido de perforacion que comprende hidrofobina.
DE102005027139A1 (de) 2005-06-10 2006-12-28 Basf Ag Neue Cystein-verarmte Hydrophobinfusionsproteine, deren Herstellung und Verwendung
DE102005048720A1 (de) 2005-10-12 2007-04-19 Basf Ag Verwendung von Proteinen als Antischaum-Komponente in Kraftstoffen
JP5354559B2 (ja) * 2005-11-24 2013-11-27 独立行政法人産業技術総合研究所 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系
CN101501432B (zh) 2006-08-15 2011-10-12 巴斯夫欧洲公司 制备干燥的自由流动的疏水蛋白制剂的方法
JP5769142B2 (ja) * 2007-09-04 2015-08-26 独立行政法人酒類総合研究所 ピヒア属酵母において異種タンパク質を高分泌させる方法
KR101498668B1 (ko) * 2008-12-04 2015-03-06 한국생명공학연구원 고분비성 단백질의 스크리닝 및 재조합 단백질의 생산을 위한 융합 파트너로서 그의 용도
US8986956B2 (en) 2010-11-04 2015-03-24 Korea Research Institute Of Bioscience And Biotechnology Method for producing human epidermal growth factor in large volume from yeast

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4588684A (en) * 1983-04-26 1986-05-13 Chiron Corporation a-Factor and its processing signals
US5081019A (en) * 1985-01-10 1992-01-14 Biogen, Inc. DNA sequences, recombinant DNA molecules and processes for producing lipocortin-like polypeptides
IT1196484B (it) * 1986-07-11 1988-11-16 Sclavo Spa Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe
WO1991005051A1 (fr) * 1989-09-28 1991-04-18 Leningradsky Gosudarstvenny Universitet Procede d'obtention de polypeptides d'interleukine-2 humaine dans des cellules de levure
AU5811194A (en) * 1992-12-11 1994-07-04 Quest International B.V. The use of the (kluyveromyces marxianus) inulinase gene promoter for protein production

Also Published As

Publication number Publication date
JP2799348B2 (ja) 1998-09-17
EP0662515A1 (de) 1995-07-12
JPH0847393A (ja) 1996-02-20
EP0662515B1 (de) 2000-11-15
US5712113A (en) 1998-01-27
DE69426289T2 (de) 2001-04-12
DE69426289D1 (de) 2000-12-21

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