ATE478140T1 - Verfahren zur herstellung sezernierter polypeptide - Google Patents

Verfahren zur herstellung sezernierter polypeptide

Info

Publication number
ATE478140T1
ATE478140T1 AT04751203T AT04751203T ATE478140T1 AT E478140 T1 ATE478140 T1 AT E478140T1 AT 04751203 T AT04751203 T AT 04751203T AT 04751203 T AT04751203 T AT 04751203T AT E478140 T1 ATE478140 T1 AT E478140T1
Authority
AT
Austria
Prior art keywords
nucleotide sequence
polypeptide
signal peptide
fungal host
operably linked
Prior art date
Application number
AT04751203T
Other languages
English (en)
Inventor
Suchindra Maiyuran
Ana Fidantsef
Howard Brody
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of ATE478140T1 publication Critical patent/ATE478140T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT04751203T 2003-05-02 2004-04-30 Verfahren zur herstellung sezernierter polypeptide ATE478140T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46776603P 2003-05-02 2003-05-02
PCT/US2004/013711 WO2004111228A1 (en) 2003-05-02 2004-04-30 Methods for producing secreted polypeptides

Publications (1)

Publication Number Publication Date
ATE478140T1 true ATE478140T1 (de) 2010-09-15

Family

ID=33551405

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04751203T ATE478140T1 (de) 2003-05-02 2004-04-30 Verfahren zur herstellung sezernierter polypeptide

Country Status (8)

Country Link
US (4) US7393664B2 (de)
EP (1) EP1625219B1 (de)
CN (1) CN1836038B (de)
AT (1) ATE478140T1 (de)
DE (1) DE602004028724D1 (de)
DK (1) DK1625219T3 (de)
ES (1) ES2350903T3 (de)
WO (1) WO2004111228A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1682656T3 (da) * 2003-10-28 2013-11-18 Novozymes Inc Polypeptider med beta-glucosidaseaktivitet og polynukleotider, der koder for dem
PT3219806T (pt) 2004-03-25 2020-06-30 Novozymes Inc Métodos para degradar ou converter polissacarídeos da parede celular de plantas
MX2007014229A (es) * 2005-05-19 2008-02-07 Novozymes North America Inc Produccion de enzimas.
US8697364B2 (en) * 2006-06-26 2014-04-15 Dsm Ip Assets B.V. High throughput transfection of filamentous fungi
WO2008057637A2 (en) * 2006-07-21 2008-05-15 Novozymes, Inc. Methods of increasing secretion of polypeptides having biological activity
US8124394B2 (en) * 2007-09-28 2012-02-28 Novozymes, A/S Polypeptides having cellobiohydrolase II activity and polynuleotides encoding same
US9012186B2 (en) 2009-04-27 2015-04-21 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
EP2397491A1 (de) 2010-06-21 2011-12-21 Technische Universität Wien LeaA von Trichoderma reesei
EP2636738A4 (de) * 2010-11-05 2014-04-30 Asahi Glass Co Ltd Transformant von hefe der gattung schizosaccharomyces und herstellungsverfahren dafür
DK2748305T3 (en) 2011-08-24 2017-02-06 Novozymes Inc CELLULOLYTIC ENZYME COMPOSITIONS AND APPLICATIONS THEREOF
CN103184159B (zh) * 2011-12-27 2016-09-21 丰益(上海)生物技术研发中心有限公司 紧密枝顶孢霉菌株及其特异性脂肪酶
EP2708553A1 (de) 2012-09-18 2014-03-19 Technische Universität Wien Modifizierte Pilzzelle
CN105229141A (zh) 2013-03-15 2016-01-06 拉勒曼德匈牙利流动管理有限责任公司 用于木质纤维素和相关低聚物的水解的β-葡糖苷酶的表达
EP3074513A1 (de) 2013-11-26 2016-10-05 Novozymes A/S Enzymzusammensetzungen und verwendungen davon
EP3152315B1 (de) 2014-06-06 2018-08-15 Novozymes A/S Enzymzusammensetzungen und ihre verwendungen
US10385352B2 (en) 2015-03-09 2019-08-20 Novozymes A/S Methods of introducing multiple expression constructs into a eukaryotic cell
CN104975039B (zh) * 2015-06-24 2018-05-11 方诩 一种重组质粒及其在降解纤维素原料中的应用
EP3369823A4 (de) * 2015-10-30 2019-04-03 Suntory Holdings Limited Verfahren zur herstellung von steviol und steviolglycosid mit einem aobgl3-homolog
US11268118B2 (en) 2015-10-30 2022-03-08 Suntory Holdings Limited Method for producing steviol and steviol glycoside using AOBGL1 homolog
EP3541954A1 (de) 2016-11-21 2019-09-25 Novozymes A/S Durch hefezellenextrakt unterstützte konstruktion von dna-molekülen
EP3564358A4 (de) * 2016-12-27 2020-12-16 Kagoshima University Technologie zur selektiven proteinsekretion in pilzen
WO2020106600A1 (en) * 2018-11-19 2020-05-28 Basf Se Leader sequence for yeast
CN112048017B (zh) * 2019-09-03 2021-04-20 南京市妇幼保健院 一种卵母细胞体外成熟培养方法
CN111378674B (zh) * 2020-03-25 2021-09-10 中国科学院天津工业生物技术研究所 异宗毁丝霉糖化酶MhglaA及其编码基因和在葡萄糖生产中的应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220721A (en) * 1979-04-27 1980-09-02 University Of Arkansas Foundation Method for enzyme reutilization
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
ES2068586T5 (es) * 1990-05-09 2004-12-01 Novozymes A/S Una preparacion de celulasa que comprende una enzima endoglucanasa.
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
EP0959128B1 (de) * 1996-07-24 2006-08-30 Meiji Seika Kabushiki Kaisha Endoglucanase-enzym und cellulase-zusammensetzungen, die dieses enthalten
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Also Published As

Publication number Publication date
DK1625219T3 (da) 2010-11-01
US20140011262A1 (en) 2014-01-09
EP1625219A4 (de) 2007-09-05
CN1836038A (zh) 2006-09-20
WO2004111228A1 (en) 2004-12-23
ES2350903T3 (es) 2011-01-28
EP1625219A1 (de) 2006-02-15
US20080268526A1 (en) 2008-10-30
DE602004028724D1 (de) 2010-09-30
EP1625219B1 (de) 2010-08-18
US8497115B2 (en) 2013-07-30
US7393664B2 (en) 2008-07-01
US20040248258A1 (en) 2004-12-09
US20120122191A1 (en) 2012-05-17
CN1836038B (zh) 2011-04-06

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