ATE214095T1 - Verwendung einer exo-nukleotidprobe in der genklonierung - Google Patents
Verwendung einer exo-nukleotidprobe in der genklonierungInfo
- Publication number
- ATE214095T1 ATE214095T1 AT92914436T AT92914436T ATE214095T1 AT E214095 T1 ATE214095 T1 AT E214095T1 AT 92914436 T AT92914436 T AT 92914436T AT 92914436 T AT92914436 T AT 92914436T AT E214095 T1 ATE214095 T1 AT E214095T1
- Authority
- AT
- Austria
- Prior art keywords
- gene cloning
- exo
- nucleotide sample
- provides methods
- present
- Prior art date
Links
- 238000012215 gene cloning Methods 0.000 title abstract 2
- 239000002773 nucleotide Substances 0.000 title 1
- 238000000034 method Methods 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/715,623 US5137814A (en) | 1991-06-14 | 1991-06-14 | Use of exo-sample nucleotides in gene cloning |
| PCT/US1992/004922 WO1992022649A1 (en) | 1991-06-14 | 1992-06-12 | Use of exo-sample nucleotides in gene cloning |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE214095T1 true ATE214095T1 (de) | 2002-03-15 |
Family
ID=24874814
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT92914436T ATE214095T1 (de) | 1991-06-14 | 1992-06-12 | Verwendung einer exo-nukleotidprobe in der genklonierung |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5137814A (de) |
| EP (2) | EP0544894B1 (de) |
| JP (1) | JP3395905B2 (de) |
| AT (1) | ATE214095T1 (de) |
| CA (1) | CA2088766A1 (de) |
| DE (1) | DE69232457T2 (de) |
| DK (1) | DK0544894T3 (de) |
| ES (1) | ES2173867T3 (de) |
| WO (1) | WO1992022649A1 (de) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5683896A (en) | 1989-06-01 | 1997-11-04 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| US5229283A (en) * | 1991-06-14 | 1993-07-20 | Life Technologies, Inc. | Use of exo-sample nucleotides in gene cloning |
| WO1993018175A1 (en) * | 1992-03-10 | 1993-09-16 | Life Technologies, Inc. | Udg-facilitated mutagenesis |
| US5426039A (en) * | 1993-09-08 | 1995-06-20 | Bio-Rad Laboratories, Inc. | Direct molecular cloning of primer extended DNA containing an alkane diol |
| ES2118534T3 (es) * | 1993-10-28 | 1998-09-16 | Life Technologies Inc | Escalera de marcadores de acido nucleico para estimacion de la masa. |
| US6680378B1 (en) * | 1993-10-28 | 2004-01-20 | Invitrogen Corporation | Nucleic acid marker ladder for estimating mass |
| US6495318B2 (en) * | 1996-06-17 | 2002-12-17 | Vectorobjects, Llc | Method and kits for preparing multicomponent nucleic acid constructs |
| EP0915903A4 (de) * | 1996-06-17 | 2002-09-04 | Biodynamics Associates | Methode und kits zur herstellung von multikomponent-nukleinsäure-konstrukten |
| IL120339A0 (en) * | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Improved DNA assembly method |
| US6365731B1 (en) | 1997-08-06 | 2002-04-02 | Ambion, Inc. | Stripping nucleic acids with iodine and sodium thiosulfate |
| US6815185B2 (en) * | 1997-11-17 | 2004-11-09 | Robert D. Klein | Methods of creating constructs useful for introducing sequences into embryonic stem cells |
| US6589737B1 (en) * | 1999-05-21 | 2003-07-08 | Invitrogen Corporation | Compositions and methods for labeling of nucleic acid molecules |
| US7537886B1 (en) | 1999-06-22 | 2009-05-26 | Life Technologies Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| US6830902B1 (en) * | 1999-07-02 | 2004-12-14 | Invitrogen Corporation | Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis |
| US6998273B1 (en) * | 2000-02-09 | 2006-02-14 | A-Fem Medical Corporation | Collection device for lateral flow chromatography |
| US6660475B2 (en) | 2000-12-15 | 2003-12-09 | New England Biolabs, Inc. | Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof |
| US20030165859A1 (en) * | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| US7435572B2 (en) | 2002-04-12 | 2008-10-14 | New England Biolabs, Inc. | Methods and compositions for DNA manipulation |
| US7273730B2 (en) * | 2002-05-24 | 2007-09-25 | Invitrogen Corporation | Nested PCR employing degradable primers |
| US7531306B2 (en) * | 2003-11-12 | 2009-05-12 | Geneohm Sciences, Inc. | Nucleic acid hybridization methods |
| US20060105348A1 (en) * | 2004-11-15 | 2006-05-18 | Lee Jun E | Compositions and methods for the detection and discrimination of nucleic acids |
| US20060275792A1 (en) * | 2004-11-15 | 2006-12-07 | Lee Jun E | Enhancement of nucleic acid amplification using double-stranded DNA binding proteins |
| US9505846B2 (en) | 2005-01-28 | 2016-11-29 | Life Technologies Corporation | Multi-component inhibitors of nucleic acid polymerases |
| US7754944B2 (en) * | 2006-04-03 | 2010-07-13 | Monsanto Technology Llc | Polynucleotide constructs |
| CN101979592B (zh) * | 2010-10-27 | 2012-05-09 | 上海交通大学 | 固氮鱼腥藻乙酰乳酸合酶基因的核苷酸序列 |
| US20150037787A1 (en) * | 2013-07-31 | 2015-02-05 | International Business Machines Corporation | Polynucleotide configuration for reliable electrical and optical sensing |
| KR102160389B1 (ko) | 2013-08-05 | 2020-09-28 | 트위스트 바이오사이언스 코포레이션 | 드 노보 합성된 유전자 라이브러리 |
| WO2016022557A1 (en) * | 2014-08-05 | 2016-02-11 | Twist Bioscience Corporation | Cell free cloning of nucleic acids |
| CA2975855C (en) | 2015-02-04 | 2025-09-23 | Twist Bioscience Corporation | SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS |
| CA2975852A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| WO2016172377A1 (en) | 2015-04-21 | 2016-10-27 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| US20160333340A1 (en) * | 2015-05-11 | 2016-11-17 | Twist Bioscience Corporation | Compositions and methods for nucleic acid amplification |
| KR20180050411A (ko) | 2015-09-18 | 2018-05-14 | 트위스트 바이오사이언스 코포레이션 | 올리고핵산 변이체 라이브러리 및 그의 합성 |
| WO2017053450A1 (en) | 2015-09-22 | 2017-03-30 | Twist Bioscience Corporation | Flexible substrates for nucleic acid synthesis |
| EP3384077A4 (de) | 2015-12-01 | 2019-05-08 | Twist Bioscience Corporation | Funktionalisierte oberflächen und herstellung davon |
| WO2018038772A1 (en) | 2016-08-22 | 2018-03-01 | Twist Bioscience Corporation | De novo synthesized nucleic acid libraries |
| EP4664304A3 (de) | 2016-09-21 | 2026-01-21 | Atlas Data Storage, Inc. | Nukleinsäurebasierte datenspeicherung |
| GB2573069A (en) | 2016-12-16 | 2019-10-23 | Twist Bioscience Corp | Variant libraries of the immunological synapse and synthesis thereof |
| WO2018156792A1 (en) | 2017-02-22 | 2018-08-30 | Twist Bioscience Corporation | Nucleic acid based data storage |
| CA3056388A1 (en) | 2017-03-15 | 2018-09-20 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| IL271205B2 (en) | 2017-06-12 | 2025-02-01 | Twist Bioscience Corp | Methods for assembling continuous nucleic acids |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| CN111566125A (zh) | 2017-09-11 | 2020-08-21 | 特韦斯特生物科学公司 | Gpcr结合蛋白及其合成 |
| CN111565834B (zh) | 2017-10-20 | 2022-08-26 | 特韦斯特生物科学公司 | 用于多核苷酸合成的加热的纳米孔 |
| IL312616B2 (en) | 2018-01-04 | 2025-08-01 | Twist Bioscience Corp | Dna-based digital information storage |
| EP3814497A4 (de) | 2018-05-18 | 2022-03-02 | Twist Bioscience Corporation | Polynukleotide, reagenzien und verfahren zur nukleinsäurehybridisierung |
| EP3902816A4 (de) | 2018-12-26 | 2022-09-14 | Twist Bioscience Corporation | Hochgenaue de-novo-polynukleotidsynthese |
| CA3131689A1 (en) | 2019-02-26 | 2020-09-03 | Twist Bioscience Corporation | Variant nucleic acid libraries for glp1 receptor |
| KR20210144698A (ko) | 2019-02-26 | 2021-11-30 | 트위스트 바이오사이언스 코포레이션 | 항체 최적화를 위한 변이 핵산 라이브러리 |
| EP3987019A4 (de) | 2019-06-21 | 2023-04-19 | Twist Bioscience Corporation | Barcode-basierte nukleinsäuresequenzanordnung |
| CN115023440B (zh) | 2019-09-23 | 2025-09-12 | 特韦斯特生物科学公司 | 结合CD3ε的抗体 |
| US12091777B2 (en) | 2019-09-23 | 2024-09-17 | Twist Bioscience Corporation | Variant nucleic acid libraries for CRTH2 |
| AU2020400030B2 (en) | 2019-12-09 | 2026-05-21 | Twist Bioscience Corporation | Variant nucleic acid libraries for adenosine receptors |
| WO2023063958A1 (en) * | 2021-10-15 | 2023-04-20 | Qiagen Sciences, Llc | Methods for producing dna libraries and uses thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4873192A (en) * | 1987-02-17 | 1989-10-10 | The United States Of America As Represented By The Department Of Health And Human Services | Process for site specific mutagenesis without phenotypic selection |
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| JPH0372882A (ja) * | 1989-05-10 | 1991-03-28 | Eastman Kodak Co | 合成二本鎖ｄｎａ配列の構築 |
| US5035996A (en) * | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
-
1991
- 1991-06-14 US US07/715,623 patent/US5137814A/en not_active Expired - Lifetime
-
1992
- 1992-06-12 AT AT92914436T patent/ATE214095T1/de not_active IP Right Cessation
- 1992-06-12 EP EP92914436A patent/EP0544894B1/de not_active Expired - Lifetime
- 1992-06-12 DE DE69232457T patent/DE69232457T2/de not_active Expired - Fee Related
- 1992-06-12 WO PCT/US1992/004922 patent/WO1992022649A1/en not_active Ceased
- 1992-06-12 EP EP01203128A patent/EP1156108A3/de not_active Withdrawn
- 1992-06-12 ES ES92914436T patent/ES2173867T3/es not_active Expired - Lifetime
- 1992-06-12 CA CA002088766A patent/CA2088766A1/en not_active Abandoned
- 1992-06-12 DK DK92914436T patent/DK0544894T3/da active
- 1992-06-12 JP JP50101693A patent/JP3395905B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DE69232457D1 (de) | 2002-04-11 |
| EP0544894A1 (de) | 1993-06-09 |
| JPH06500702A (ja) | 1994-01-27 |
| WO1992022649A1 (en) | 1992-12-23 |
| EP1156108A2 (de) | 2001-11-21 |
| EP0544894B1 (de) | 2002-03-06 |
| DE69232457T2 (de) | 2002-10-10 |
| US5137814A (en) | 1992-08-11 |
| EP0544894A4 (en) | 1994-10-19 |
| CA2088766A1 (en) | 1992-12-15 |
| DK0544894T3 (da) | 2002-04-22 |
| ES2173867T3 (es) | 2002-11-01 |
| JP3395905B2 (ja) | 2003-04-14 |
| EP1156108A3 (de) | 2001-12-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |