ATE217027T1 - Primers zur genamplifikation von enterotoxin und lecithinase aus clostridium perfringens und deren anwendung zur nachweis und zählung dieser bakterien - Google Patents

Primers zur genamplifikation von enterotoxin und lecithinase aus clostridium perfringens und deren anwendung zur nachweis und zählung dieser bakterien

Info

Publication number
ATE217027T1
ATE217027T1 AT95905106T AT95905106T ATE217027T1 AT E217027 T1 ATE217027 T1 AT E217027T1 AT 95905106 T AT95905106 T AT 95905106T AT 95905106 T AT95905106 T AT 95905106T AT E217027 T1 ATE217027 T1 AT E217027T1
Authority
AT
Austria
Prior art keywords
lecithinase
enterotoxin
bacteria
clostridium perfringens
primers
Prior art date
Application number
AT95905106T
Other languages
English (en)
Inventor
Patrick Fach
Jean-Pierre Guillou
Michel Popoff
Original Assignee
Pasteur Institut
Afssa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut, Afssa filed Critical Pasteur Institut
Application granted granted Critical
Publication of ATE217027T1 publication Critical patent/ATE217027T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT95905106T 1993-12-22 1994-12-22 Primers zur genamplifikation von enterotoxin und lecithinase aus clostridium perfringens und deren anwendung zur nachweis und zählung dieser bakterien ATE217027T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/172,026 US5538851A (en) 1993-12-22 1993-12-22 Primers for the amplification of genes coding for the enterotoxin and the lecithinase of Clostridium perfringens and their application to the determination of the presence and numeration of these bacteriae
PCT/EP1994/004292 WO1995017521A2 (en) 1993-12-22 1994-12-22 Primers for the amplification of genes coding for the enterotoxin and the lecithinase of clostridium perfringens and their application to the detection and numeration of these bacteriae

Publications (1)

Publication Number Publication Date
ATE217027T1 true ATE217027T1 (de) 2002-05-15

Family

ID=22626066

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95905106T ATE217027T1 (de) 1993-12-22 1994-12-22 Primers zur genamplifikation von enterotoxin und lecithinase aus clostridium perfringens und deren anwendung zur nachweis und zählung dieser bakterien

Country Status (5)

Country Link
US (2) US5538851A (de)
EP (1) EP0739420B1 (de)
AT (1) ATE217027T1 (de)
DE (1) DE69430539T2 (de)
WO (1) WO1995017521A2 (de)

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US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
EP0892054B1 (de) * 1997-06-20 2006-11-29 Intervet International BV Clostridium perfringens Impfstoff
AU2002301780B2 (en) * 1997-09-19 2007-02-08 Institut Pasteur Clostridium toxin and method for preparing immunogenic compositions
FR2768747B1 (fr) * 1997-09-19 2000-12-01 Pasteur Institut Acides nucleiques, cellules recombinantes, et procede de preparation de compositions immunogenes
EP1155147B1 (de) 1999-02-25 2006-06-14 EXACT Sciences Corporation Verfahren zur erhaltung der dns-integrität
JP4794052B2 (ja) 1999-04-09 2011-10-12 ジェンザイム・コーポレーション 癌の指標である核酸を検出するための方法
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US7368233B2 (en) * 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US6811549B2 (en) 2001-02-16 2004-11-02 William H. Fleming Administration of therapeutic or diagnostic agents using interlabial pad
WO2003071252A2 (en) 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
AU2002249514B8 (en) * 2002-03-26 2008-10-09 Council Of Scientific And Industrial Research Primers for detecting food poisoning bacteria and a use thereof
US20040248089A1 (en) * 2002-03-27 2004-12-09 Banada Padmanabha Padmapriya Primes for detecting food poisoning bacteria and a method thereof
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
US7232440B2 (en) * 2003-11-17 2007-06-19 Sherwood Services Ag Bipolar forceps having monopolar extension
WO2005113769A1 (en) * 2004-05-14 2005-12-01 Exact Sciences Corporation Method for stabilizing biological samples for nucleic acid analysis
US8398606B2 (en) * 2007-02-28 2013-03-19 QuantrRx Biomedical Corporation Folded perineal pad
US10058306B2 (en) 2012-06-22 2018-08-28 Preprogen, LLC Method for obtaining fetal cells and fetal cellular components
EP3622082B1 (de) 2017-05-12 2023-03-29 Evonik Operations GmbH Verfahren zum nachweis von durch c. perfringens induzierten erkrankungen in einem geflügelschwarm
WO2018206690A1 (en) * 2017-05-12 2018-11-15 Evonik Degussa Gmbh Method for detecting c. perfringens induced diseases in animals
KR20200128413A (ko) 2018-03-02 2020-11-12 에보니크 오퍼레이션즈 게엠베하 오보트랜스페린을 결정함으로써 동물에서의 장 장벽 기능상실을 검출하기 위한 시험관내 방법
MX2021006917A (es) 2018-12-14 2021-07-07 Evonik Operations Gmbh Procedimiento in vitro para la deteccion de disbiosis intestinal aviar.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE81145B1 (en) * 1989-04-20 2000-05-03 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences
JPH0789959B2 (ja) * 1989-07-18 1995-10-04 株式会社島津製作所 ウェルシュ菌検出のためのオリゴヌクレオチドおよびそれを用いた検出法
DE69032778T2 (de) * 1989-07-18 1999-07-29 Shimadzu Corp., Kyoto Verfahren zur Untersuchung von durch Mikroorganismen verursachten Lebensmittelvergiftungen und Reagens dafür

Also Published As

Publication number Publication date
US5538851A (en) 1996-07-23
EP0739420A1 (de) 1996-10-30
EP0739420B1 (de) 2002-05-02
WO1995017521A3 (en) 1995-11-16
WO1995017521A2 (en) 1995-06-29
DE69430539D1 (de) 2002-06-06
US5874220A (en) 1999-02-23
DE69430539T2 (de) 2002-12-19

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