ATE513921T1 - Verfahren zur herstellung reifer insulinpolypeptide - Google Patents

Verfahren zur herstellung reifer insulinpolypeptide

Info

Publication number
ATE513921T1
ATE513921T1 AT06778234T AT06778234T ATE513921T1 AT E513921 T1 ATE513921 T1 AT E513921T1 AT 06778234 T AT06778234 T AT 06778234T AT 06778234 T AT06778234 T AT 06778234T AT E513921 T1 ATE513921 T1 AT E513921T1
Authority
AT
Austria
Prior art keywords
human insulin
insulin polypeptides
mature insulin
producing mature
chain
Prior art date
Application number
AT06778234T
Other languages
English (en)
Inventor
Asser Sloth Andersen
Lars Hojlund Christensen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE513921T1 publication Critical patent/ATE513921T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Diabetes (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT06778234T 2005-08-16 2006-08-15 Verfahren zur herstellung reifer insulinpolypeptide ATE513921T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05107513 2005-08-16
PCT/EP2006/065303 WO2007020256A1 (en) 2005-08-16 2006-08-15 Method for making mature insulin polypeptides

Publications (1)

Publication Number Publication Date
ATE513921T1 true ATE513921T1 (de) 2011-07-15

Family

ID=35169284

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06778234T ATE513921T1 (de) 2005-08-16 2006-08-15 Verfahren zur herstellung reifer insulinpolypeptide

Country Status (7)

Country Link
US (1) US9056921B2 (de)
EP (1) EP1917363B1 (de)
JP (2) JP5366546B2 (de)
CN (2) CN102660614A (de)
AT (1) ATE513921T1 (de)
ES (1) ES2368500T3 (de)
WO (1) WO2007020256A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102660614A (zh) 2005-08-16 2012-09-12 诺沃-诺迪斯克有限公司 制备成熟胰岛素多肽的方法
EP2069502B1 (de) 2006-09-27 2014-02-26 Novo Nordisk A/S Verfahren zur herstellung gereifter insulinpolypeptide
EP2084179B1 (de) 2006-11-22 2013-05-22 Novo Nordisk A/S Verfahren zur herstellung aktivierter carboxypeptidasen
US20130295605A1 (en) * 2010-05-28 2013-11-07 Csir Method for producing a polypeptide in yarrowia lipolytica
EP3098235A4 (de) * 2014-01-20 2017-10-18 Hanmi Pharm. Co., Ltd. Langwirkendes insulin und verwendung davon
AU2015328222B9 (en) 2014-10-06 2020-12-24 Case Western Reserve University Biphasic single-chain insulin analogues
PL233560B1 (pl) 2014-12-05 2019-10-31 Mabion Spolka Akcyjna Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka
UY36870A (es) 2015-08-28 2017-03-31 Hanmi Pharm Ind Co Ltd Análogos de insulina novedosos
RU2764197C1 (ru) 2016-09-23 2022-01-14 Ханми Фарм. Ко., Лтд. Аналоги инсулина с пониженной аффинностью к рецептору инсулина и их применение
MY200047A (en) * 2016-11-21 2023-12-06 Univ Case Western Reserve Rapid-acting insulin analogues of enhanced stability
AR111341A1 (es) 2017-03-23 2019-07-03 Hanmi Pharm Ind Co Ltd Un conjugado del análogo de insulina con afinidad reducida para el receptor de insulina y uso del mismo
SG11202106167XA (en) 2018-12-11 2021-07-29 Sanofi Sa Insulin conjugates

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US4546082A (en) 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
US4599311A (en) 1982-08-13 1986-07-08 Kawasaki Glenn H Glycolytic promotersfor regulated protein expression: protease inhibitor
US4914026A (en) 1983-04-07 1990-04-03 Chiron Corporation Alpha factor leader sequence directed secretion of insulin
US4870008A (en) 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
AU600885B2 (en) 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs
US4931373A (en) 1984-05-25 1990-06-05 Zymogenetics, Inc. Stable DNA constructs for expression of α-1 antitrypsin
DK58285D0 (da) 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
US4766073A (en) 1985-02-25 1988-08-23 Zymogenetics Inc. Expression of biologically active PDGF analogs in eucaryotic cells
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
PH25772A (en) 1985-08-30 1991-10-18 Novo Industri As Insulin analogues, process for their preparation
AU6543286A (en) 1985-10-25 1987-05-19 Zymogenetics Inc. Method of using bar1 for secreting foreign proteins
US4882279A (en) 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
DK463887D0 (da) 1987-09-07 1987-09-07 Novo Industri As Gaerleader
KR900701842A (ko) 1988-07-20 1990-12-04 헨리 브뢰늄 인간 인슐린 동족체와 그를 포함하는 제제
US5037743A (en) 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
KR910700262A (ko) 1988-12-23 1991-03-14 안네 제케르 사람 인슐린 유사체
US5716927A (en) * 1988-12-23 1998-02-10 Novo Nordisk A/S Insulin analogs having a modified B-chain
DK105489D0 (da) 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
PT93057B (pt) 1989-02-09 1995-12-29 Lilly Co Eli Processo para a preparacao de analogos da insulina
AU631868B2 (en) 1989-04-20 1992-12-10 Mount Sinai School Of Medicine Of The City University Of New York, The Hepatospecific insulin analogues
DK300090D0 (da) 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
US6348327B1 (en) * 1991-12-06 2002-02-19 Genentech, Inc. Non-endocrine animal host cells capable of expressing variant proinsulin and processing the same to form active, mature insulin and methods of culturing such cells
DK82893D0 (da) 1993-07-08 1993-07-08 Novo Nordisk As Peptid
US6011007A (en) 1993-09-17 2000-01-04 Novo Nordisk A/S Acylated insulin
CZ287945B6 (cs) 1993-09-17 2001-03-14 Novo Nordisk A/S Inzulinový derivát a farmaceutický prostředek s jeho obsahem pro léčení diabetu
US5639642A (en) 1994-06-16 1997-06-17 Novo Nordisk A/S Synthetic leader peptide sequences
ZA954983B (en) 1994-06-17 1996-02-14 Novo Nordisk As N-terminally extended proteins expressed in yeast
US5646242A (en) 1994-11-17 1997-07-08 Eli Lilly And Company Selective acylation of epsilon-amino groups
US5693609A (en) 1994-11-17 1997-12-02 Eli Lilly And Company Acylated insulin analogs
GB9513967D0 (en) * 1995-07-08 1995-09-06 Univ Leicester Insulin
US20030104981A1 (en) * 1995-11-03 2003-06-05 Jelena Mandic Human insulin analogues
US6451970B1 (en) 1996-02-21 2002-09-17 Novo Nordisk A/S Peptide derivatives
IL127583A0 (en) 1996-07-05 1999-10-28 Novo Nordisk As Method for the production of polypeptides
JP2001527387A (ja) 1997-01-24 2001-12-25 ノボ ノルディスク アクティーゼルスカブ 合成リーダーペプチド配列
EP1246933B1 (de) 1999-12-29 2010-07-21 Novo Nordisk A/S VERFAHREN ZUR HERSTELLUNG VON INSULINVORLäUFERN UND ANALOGEN VON INSULINVORLÄUFERN MIT VERBESSERTER FERMENTATIONSAUSBEUTE IN HEFE
EP1377608B1 (de) 2001-04-02 2009-09-16 Novo Nordisk A/S Insulinvorstufen und verfahren zu deren herstellung
CN102660614A (zh) 2005-08-16 2012-09-12 诺沃-诺迪斯克有限公司 制备成熟胰岛素多肽的方法
EP2069502B1 (de) 2006-09-27 2014-02-26 Novo Nordisk A/S Verfahren zur herstellung gereifter insulinpolypeptide
EP2084179B1 (de) * 2006-11-22 2013-05-22 Novo Nordisk A/S Verfahren zur herstellung aktivierter carboxypeptidasen

Also Published As

Publication number Publication date
US20110111460A1 (en) 2011-05-12
ES2368500T3 (es) 2011-11-17
JP2009504169A (ja) 2009-02-05
WO2007020256A1 (en) 2007-02-22
EP1917363A1 (de) 2008-05-07
JP5366546B2 (ja) 2013-12-11
CN101243190A (zh) 2008-08-13
EP1917363B1 (de) 2011-06-22
JP2013208119A (ja) 2013-10-10
CN102660614A (zh) 2012-09-12
CN101243190B (zh) 2015-05-13
US9056921B2 (en) 2015-06-16

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