ATE231182T1 - Methode zur erhöhung der plasmid ausbeute - Google Patents

Methode zur erhöhung der plasmid ausbeute

Info

Publication number
ATE231182T1
ATE231182T1 AT95912828T AT95912828T ATE231182T1 AT E231182 T1 ATE231182 T1 AT E231182T1 AT 95912828 T AT95912828 T AT 95912828T AT 95912828 T AT95912828 T AT 95912828T AT E231182 T1 ATE231182 T1 AT E231182T1
Authority
AT
Austria
Prior art keywords
medium
plasmid yield
increase plasmid
plasmid
growth
Prior art date
Application number
AT95912828T
Other languages
English (en)
Inventor
Nick C Wan
Jason C Goodrick
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Application granted granted Critical
Publication of ATE231182T1 publication Critical patent/ATE231182T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT95912828T 1994-03-10 1995-03-10 Methode zur erhöhung der plasmid ausbeute ATE231182T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/209,581 US5487986A (en) 1994-03-10 1994-03-10 Method for increasing plasmid yield
PCT/US1995/002949 WO1996006925A1 (en) 1994-03-10 1995-03-10 Method for increasing plasmid yield

Publications (1)

Publication Number Publication Date
ATE231182T1 true ATE231182T1 (de) 2003-02-15

Family

ID=22779351

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95912828T ATE231182T1 (de) 1994-03-10 1995-03-10 Methode zur erhöhung der plasmid ausbeute

Country Status (8)

Country Link
US (1) US5487986A (de)
EP (1) EP0751986B1 (de)
JP (1) JPH09510109A (de)
AT (1) ATE231182T1 (de)
AU (1) AU699919B2 (de)
CA (1) CA2183649A1 (de)
DE (1) DE69529406D1 (de)
WO (1) WO1996006925A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10113815A1 (de) * 2001-03-21 2002-10-02 Eppendorf Ag Verfahren zur Isolierung von Plasmiden oder Proteinen aus suspendierten Bakterien- oder Hefezellen
US7299805B2 (en) 2002-06-07 2007-11-27 Marctec, Llc Scaffold and method for implanting cells
EP1584680A1 (de) * 2004-04-08 2005-10-12 Boehringer Ingelheim Austria GmbH Fed-Batch Fermentationsverfahren zur Herstellung von Plasmid-DNA
US20050233365A1 (en) * 2004-04-08 2005-10-20 Boehringer Ingelheim Austria Gmbh Method for producing plasmid DNA on a manufacturing scale
US20110269184A1 (en) 2009-07-16 2011-11-03 Boehringer Ingelheim Rcv Gmbh & Co. Kg Method for controlling plasmid copy number in e.coli
WO2019018270A1 (en) 2017-07-21 2019-01-24 Conagen, Inc. PLASMID DEPENDENCE SYSTEM FOR DIRECTING THE EXPRESSION OF A DESIRED GENE
KR20220066384A (ko) * 2019-09-25 2022-05-24 카톨리에케 유니버시테이트 루벤 대형 벡터 및 고수율 제조를 위한 방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3753723A (en) * 1970-07-31 1973-08-21 H Henderson Animal feed and method of making same
JPS57170184A (en) * 1980-07-18 1982-10-20 Unisearch Ltd Mutant of e. coli and production of phenylalanine
JPS60118196A (ja) * 1983-11-30 1985-06-25 Takeda Chem Ind Ltd インタ−フェロンの製造法
GB2155935B (en) * 1984-02-16 1988-10-12 Antibioticos Sa Novel e. coli hybrid plasmid vector conferring sucrose fermenting capacity
DE3614586A1 (de) * 1986-05-02 1987-11-05 Degussa Verfahren zur fermentativen herstellung von l-aminosaeuren aus (alpha)-ketocarbonsaeuren
JPH0728749B2 (ja) * 1986-09-22 1995-04-05 協和醗酵工業株式会社 Ｌ−アルギニンの製造法
US5032514A (en) * 1988-08-08 1991-07-16 Genentech, Inc. Metabolic pathway engineering to increase production of ascorbic acid intermediates
US5219746A (en) * 1990-07-19 1993-06-15 Chris Brinegar Ice-mediated introduction of substances into biological material
JP3006926B2 (ja) * 1991-09-04 2000-02-07 協和醗酵工業株式会社 発酵法によるｌ−スレオニンの製造法

Also Published As

Publication number Publication date
CA2183649A1 (en) 1996-03-07
JPH09510109A (ja) 1997-10-14
EP0751986A1 (de) 1997-01-08
WO1996006925A1 (en) 1996-03-07
EP0751986B1 (de) 2003-01-15
AU1986095A (en) 1996-03-22
DE69529406D1 (de) 2003-02-20
US5487986A (en) 1996-01-30
EP0751986A4 (de) 1997-11-26
AU699919B2 (en) 1998-12-17

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