ATE343137T1 - Verfahren zur bestimmung von polypeptiden - Google Patents

Verfahren zur bestimmung von polypeptiden

Info

Publication number
ATE343137T1
ATE343137T1 AT02735602T AT02735602T ATE343137T1 AT E343137 T1 ATE343137 T1 AT E343137T1 AT 02735602 T AT02735602 T AT 02735602T AT 02735602 T AT02735602 T AT 02735602T AT E343137 T1 ATE343137 T1 AT E343137T1
Authority
AT
Austria
Prior art keywords
polypeptide
capping
peptide fragments
free thiols
cleavage reagent
Prior art date
Application number
AT02735602T
Other languages
English (en)
Inventor
Christian Hamon
Andrew Thompson
Thomas Neumann
Robert Johnstone
Abdul Karim Abed Mohammed
Original Assignee
Electrophoretics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP01304975A external-priority patent/EP1267170A1/de
Priority claimed from EP01306842A external-priority patent/EP1265072A1/de
Application filed by Electrophoretics Ltd filed Critical Electrophoretics Ltd
Application granted granted Critical
Publication of ATE343137T1 publication Critical patent/ATE343137T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6821—Sequencing of polypeptides involving C-terminal degradation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT02735602T 2001-06-07 2002-06-07 Verfahren zur bestimmung von polypeptiden ATE343137T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01304975A EP1267170A1 (de) 2001-06-07 2001-06-07 Verfahren zur Charakterisierung von Polypeptiden
EP01306842A EP1265072A1 (de) 2001-06-07 2001-08-10 Verfahren zur Charakterisierung von Polypeptiden
EP02250740 2002-02-04

Publications (1)

Publication Number Publication Date
ATE343137T1 true ATE343137T1 (de) 2006-11-15

Family

ID=27224353

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02735602T ATE343137T1 (de) 2001-06-07 2002-06-07 Verfahren zur bestimmung von polypeptiden

Country Status (9)

Country Link
US (1) US7163803B2 (de)
EP (1) EP1397686B1 (de)
JP (1) JP4163103B2 (de)
AT (1) ATE343137T1 (de)
CA (1) CA2449680A1 (de)
DE (1) DE60215498T2 (de)
NO (1) NO20035402D0 (de)
NZ (1) NZ529985A (de)
WO (1) WO2002099435A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1267170A1 (de) * 2001-06-07 2002-12-18 Xzillion GmbH & CO.KG Verfahren zur Charakterisierung von Polypeptiden
AU2003216920A1 (en) 2002-04-08 2003-10-20 Glaxo Group Limited (2-((2-alkoxy)-phenyl)-cyclopent-1-enyl) aromatic carbo and heterocyclic acid and derivatives
GB0225548D0 (en) 2002-11-01 2002-12-11 Glaxo Group Ltd Compounds
KR101113201B1 (ko) * 2003-02-19 2012-04-12 나트릭스 세퍼레이션즈, 인코포레이티드 지지된 다공성 젤을 함유하는 복합재
GB0306756D0 (en) 2003-03-24 2003-04-30 Xzillion Gmbh & Co Kg Mass labels
GB0317123D0 (en) * 2003-07-22 2003-08-27 Xzillion Gmbh & Co Kg Characterising polypeptides
WO2005075993A1 (en) * 2004-02-10 2005-08-18 Korea Basic Science Institute Selective labeling agent for phosphoproteome analysis and phosphorylated site analysis
WO2005097304A1 (en) * 2004-04-08 2005-10-20 Mcmaster University Membrane stacks
CA2564413C (en) * 2004-06-07 2013-10-01 Mcmaster University Stable composite material comprising supported porous gels
US7476546B2 (en) * 2004-11-15 2009-01-13 University Of North Dakota Method for single oxygen atom incorporation into digested peptides using peptidases
GB0518585D0 (en) 2005-09-12 2005-10-19 Electrophoretics Ltd Mass labels
US8580534B2 (en) 2006-06-30 2013-11-12 The University Of North Dakota Method for incorporation of two oxygen atoms into digested peptides using peptidases
US8859734B2 (en) 2006-09-29 2014-10-14 Intel Corporation Method for the selective enrichment and labeling of phosphorproteins
WO2009085106A1 (en) * 2007-12-27 2009-07-09 Duke University Resin assisted capture of cysteine-modified proteins/peptides and determination of presence and location of modification
US20110151494A1 (en) * 2008-06-30 2011-06-23 H. Lee Moffit Cancer & Research Institute Methods and materials for monitoring myeloma using quantitative mass spectrometry
AU2009288234B2 (en) 2008-09-02 2014-08-21 Merck Millipore Ltd. Chromatography membranes, devices containing them, and methods of use thereof
EP2499192B1 (de) * 2009-11-13 2020-07-15 Merck Millipore Ltd. Membranen für hydrophobe interaktionschromatographie
ES2968249T3 (es) 2011-05-17 2024-05-08 Merck Millipore Ltd Dispositivo con membranas tubulares en capas para cromatografía
EP4036579B1 (de) 2013-03-15 2025-05-07 Arizona Board of Regents on behalf of Arizona State University Biosensor-mikroarrayzusammensetzungen und verfahren
US9146219B2 (en) * 2014-01-27 2015-09-29 National Medical Services, Inc. Sensitive method for measuring cis-diol containing compounds in plasma using 2D-LC-MS/MS
US12176196B2 (en) * 2019-02-15 2024-12-24 Ohio State Innovation Foundation Surface-induced dissociation devices and methods
CN113252814A (zh) * 2021-05-25 2021-08-13 上海应用技术大学 一种大豆蛋白水解物苦味肽的鉴定方法
WO2023003882A2 (en) * 2021-07-19 2023-01-26 University Of North Carolina At Wilmington Compounds having selective inactivation activity

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0669399B2 (ja) 1988-03-15 1994-09-07 三菱化成株式会社 カルボキシル末端ペプチドの分取方法
JPH0648017B2 (ja) * 1988-07-12 1994-06-22 株式会社大金製作所 ダンパーディスク
US5470703A (en) 1992-10-21 1995-11-28 Shimadzu Corporation Method for peptide C-terminal fragment sequence analysis and apparatus for collecting peptide fragment
JP3353278B2 (ja) * 1992-12-24 2002-12-03 株式会社島津製作所 ペプチドｎ末端フラグメントの分取方法
JPH07165789A (ja) 1993-12-15 1995-06-27 Toray Res Center:Kk タンパク質のアミノ末端ペプチドの分離方法
US5750360A (en) * 1995-06-07 1998-05-12 Lxr Biotechnology Inc. Method for quantitatively measuring apoptosis
IL130949A (en) * 1997-01-23 2004-12-15 Xzillion Gmbh & Co Kg Method for characterising polypeptides
GB9821393D0 (en) 1998-10-01 1998-11-25 Brax Genomics Ltd Protein profiling 2
AUPQ664300A0 (en) * 2000-04-03 2000-05-04 Proteome Systems Ltd Macromolecule detection
US20060141632A1 (en) * 2000-07-25 2006-06-29 The Procter & Gamble Co. New methods and kits for sequencing polypeptides
EP1265072A1 (de) * 2001-06-07 2002-12-11 Xzillion GmbH & CO.KG Verfahren zur Charakterisierung von Polypeptiden
EP1267170A1 (de) * 2001-06-07 2002-12-18 Xzillion GmbH & CO.KG Verfahren zur Charakterisierung von Polypeptiden

Also Published As

Publication number Publication date
NO20035402D0 (no) 2003-12-05
JP4163103B2 (ja) 2008-10-08
US20050095661A1 (en) 2005-05-05
DE60215498T2 (de) 2007-04-26
WO2002099435A1 (en) 2002-12-12
US7163803B2 (en) 2007-01-16
NZ529985A (en) 2006-02-24
JP2004537716A (ja) 2004-12-16
DE60215498D1 (de) 2006-11-30
EP1397686A1 (de) 2004-03-17
EP1397686B1 (de) 2006-10-18
CA2449680A1 (en) 2002-12-12

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