ATE237684T1 - Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae) - Google Patents
Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae)Info
- Publication number
- ATE237684T1 ATE237684T1 AT93915201T AT93915201T ATE237684T1 AT E237684 T1 ATE237684 T1 AT E237684T1 AT 93915201 T AT93915201 T AT 93915201T AT 93915201 T AT93915201 T AT 93915201T AT E237684 T1 ATE237684 T1 AT E237684T1
- Authority
- AT
- Austria
- Prior art keywords
- proteins
- recombinant
- saccharomyces cerevisiae
- pdi
- strains
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 title 1
- 102000006010 Protein Disulfide-Isomerase Human genes 0.000 abstract 3
- 125000002228 disulfide group Chemical group 0.000 abstract 3
- 108020003519 protein disulfide isomerase Proteins 0.000 abstract 3
- 101001072202 Homo sapiens Protein disulfide-isomerase Proteins 0.000 abstract 1
- 102000018697 Membrane Proteins Human genes 0.000 abstract 1
- 108010052285 Membrane Proteins Proteins 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 102000040739 Secretory proteins Human genes 0.000 abstract 1
- 108091058545 Secretory proteins Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000010276 construction Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 230000003248 secreting effect Effects 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/901,713 US6291205B1 (en) | 1992-06-12 | 1992-06-12 | Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae |
| PCT/US1993/005318 WO1993025676A1 (en) | 1992-06-12 | 1993-06-02 | METHOD FOR INCREASING PRODUCTION OF DISULFIDE BONDED RECOMBINANT PROTEINS BY $i(SACCHAROMYCES CEREVISIAE) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE237684T1 true ATE237684T1 (de) | 2003-05-15 |
Family
ID=25414688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT93915201T ATE237684T1 (de) | 1992-06-12 | 1993-06-02 | Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae) |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US6291205B1 (de) |
| EP (1) | EP0746611B1 (de) |
| JP (3) | JP3953094B2 (de) |
| KR (1) | KR0171205B1 (de) |
| AT (1) | ATE237684T1 (de) |
| AU (1) | AU679448B2 (de) |
| CA (1) | CA2137142C (de) |
| DE (1) | DE69332893T2 (de) |
| DK (1) | DK0746611T3 (de) |
| ES (1) | ES2194848T3 (de) |
| NZ (1) | NZ253873A (de) |
| WO (1) | WO1993025676A1 (de) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU218419B (hu) * | 1992-03-06 | 2000-08-28 | E.R. Squibb And Sons, Inc. | Mikroszomális triglicerid transzfer protein (MTP) nagy molekulatömegű alegységének rekombináns úton történő előállítására és a protein és inhibitorainak kimutatására szolgáló eljárások |
| DK76893D0 (de) | 1993-06-28 | 1993-06-28 | Novo Nordisk As | |
| DK0635574T3 (da) * | 1993-07-23 | 2003-08-11 | Dsm Ip Assets Bv | Selektionsmarkørgenfri rekombinantstammer, en fremgangsmåde til fremstilling af disse og anvendelse af disse stammer |
| US5639635A (en) * | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US5789199A (en) * | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
| AU2660397A (en) * | 1996-04-05 | 1997-10-29 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells |
| EP0828004B1 (de) * | 1996-09-04 | 2006-11-29 | Suntory Limited | Protein-disulfidisomerase-Gen eines methylotrophen Hefestammes |
| GB9703406D0 (en) | 1997-02-19 | 1997-04-09 | Chiron Spa | Expression of heterologous proteins |
| US6083715A (en) * | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
| DK1571217T3 (da) * | 1997-07-16 | 2009-07-06 | Genencor Int | Ögende produktion af proteiner i gram-positive mikroorganismer |
| US6521421B1 (en) | 1997-07-16 | 2003-02-18 | Genencor International, Inc. | Expression vectors encoding Bacillus subtilis disulfide bond isomerase and methods of secreting proteins in gram-positive microorganisms using the same |
| US7037684B2 (en) * | 2000-03-14 | 2006-05-02 | Itoham Foods Inc. | Process for producing polypeptide having disulfide bond |
| AU2002351353A1 (en) * | 2001-12-19 | 2003-09-02 | Genentech, Inc. | Stabilizing polypeptides which have been exposed to urea |
| US20040203132A1 (en) | 2003-02-28 | 2004-10-14 | Cognetix, Inc. | Conus protein disulfide isomerase |
| US7244616B2 (en) | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
| GB0329681D0 (en) | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Gene expression technique |
| GB0329722D0 (en) * | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Modified plasmid and use thereof |
| AU2005317828A1 (en) | 2004-12-23 | 2006-06-29 | Novozymes Delta Limited | Gene expression technique |
| GB0512707D0 (en) * | 2005-06-22 | 2005-07-27 | Delta Biotechnology Ltd | Gene expression technique |
| US20110034396A1 (en) * | 2005-09-28 | 2011-02-10 | Biovascular, Inc. | Methods and compositions for inhibiting cell migration and treatment of inflammatory conditions |
| EP1931733A4 (de) * | 2005-09-28 | 2009-11-25 | Biovascular Inc | Verfahren und zusammensetzungen zum blockieren von thrombozyten- und zelladhäsion, zellmigration und entzündung |
| JP2013009685A (ja) * | 2005-11-24 | 2013-01-17 | National Institute Of Advanced Industrial Science & Technology | 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系 |
| EP2041280B1 (de) * | 2006-06-20 | 2016-08-10 | Mogam Biotechnology Research Institute | Verfahren zur verbesserung der sekretionseffizienz eines rekombinanten fremdproteins im hefeexpressionssystem |
| WO2009019368A2 (fr) * | 2007-07-19 | 2009-02-12 | bioMérieux | Procede de dosage de la liver fatty acid-binding protein, de l'ace et du ca19-9 pour le diagnostic in vitro du cancer colorectal |
| FR2919062B1 (fr) * | 2007-07-19 | 2009-10-02 | Biomerieux Sa | Procede de dosage de l'aminoacylase 1 pour le diagnostic in vitro du cancer colorectal. |
| FR2919063B1 (fr) | 2007-07-19 | 2009-10-02 | Biomerieux Sa | Procede de dosage du leucocyte elastase inhibitor pour le diagnostic in vitro du cancer colorectal. |
| FR2919064B1 (fr) * | 2007-07-19 | 2009-10-02 | Biomerieux Sa | Procede de dosage de l'apolipoproteine all pour le diagnostic in vitro du cancer colorectal |
| FR2919060B1 (fr) * | 2007-07-19 | 2012-11-30 | Biomerieux Sa | Procede de dosage de l'ezrine pour le diagnostic in vitro du cancer colorectal. |
| FR2919065B1 (fr) | 2007-07-19 | 2009-10-02 | Biomerieux Sa | Procede de dosage de l'apolipoproteine ai pour le diagnostic in vitro du cancer colorectal |
| FR2919061B1 (fr) | 2007-07-19 | 2009-10-02 | Biomerieux Sa | Procede de dosage de la plastine-i pour le diagnostic in vitro du cancer colorectal. |
| JP5288379B2 (ja) † | 2007-10-31 | 2013-09-11 | 独立行政法人産業技術総合研究所 | タンパク質の高分泌生産方法 |
| AU2009215739A1 (en) * | 2008-02-20 | 2009-08-27 | Glycofi, Inc. | Vectors and yeast strains for protein production |
| FR2933773B1 (fr) * | 2008-07-10 | 2013-02-15 | Biomerieux Sa | Procede de dosage de la proteine disulfide isomerase pour le diagnostic in vitro du cancer colorectal |
| EP2258854A1 (de) | 2009-05-20 | 2010-12-08 | FH Campus Wien | Eukaryotische Wirtszelle mit einem Ausdrucksverstärker |
| EP2565262A1 (de) | 2011-08-31 | 2013-03-06 | VTU Holding GmbH | Protein-Expression |
| RU2663587C2 (ru) | 2013-03-06 | 2018-08-07 | ГЛЭКСОСМИТКЛАЙН ЭлЭлСи | Клетки-хозяева и способы использования |
| JP2017511147A (ja) | 2014-04-17 | 2017-04-20 | ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト | 目的タンパク質を発現させるための組換え宿主細胞 |
| AU2015248815B2 (en) | 2014-04-17 | 2021-07-08 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Recombinant host cell engineered to overexpress helper proteins |
| KR102699023B1 (ko) | 2018-11-08 | 2024-08-27 | 서트로 바이오파마, 인크. | 산화성 세포질을 갖는 대장균 균주 |
| US12351850B2 (en) | 2020-04-30 | 2025-07-08 | Sutro Biopharma, Inc. | Methods of producing full-length antibodies using E. coli |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1988005816A1 (fr) * | 1987-01-28 | 1988-08-11 | Takeda Chemical Industries, Ltd. | Polypeptide |
| IL86455A0 (en) | 1987-06-01 | 1988-11-15 | Takeda Chemical Industries Ltd | Polypeptide and production thereof |
| JPH0638771A (ja) * | 1990-10-31 | 1994-02-15 | Tonen Corp | ヒトプロテインジスルフィドイソメラーゼ遺伝子の発現方法および該遺伝子との共発現によるポリペプチドの製造方法 |
| JPH05317037A (ja) * | 1991-10-30 | 1993-12-03 | Tonen Corp | 酵母ｅｒｄ２遺伝子とそのリガンドとしての小胞体局在蛋白質をコードする遺伝子とを含む共発現系及びそれを利用する有用ポリペプチドの製造法 |
| EP0509841A3 (en) * | 1991-04-18 | 1993-08-18 | Tonen Corporation | Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system |
| DE4113750A1 (de) * | 1991-04-26 | 1992-10-29 | Boehringer Mannheim Gmbh | Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen |
-
1992
- 1992-06-12 US US07/901,713 patent/US6291205B1/en not_active Expired - Lifetime
-
1993
- 1993-06-02 NZ NZ253873A patent/NZ253873A/en not_active IP Right Cessation
- 1993-06-02 JP JP50158794A patent/JP3953094B2/ja not_active Expired - Lifetime
- 1993-06-02 AU AU45274/93A patent/AU679448B2/en not_active Expired
- 1993-06-02 CA CA002137142A patent/CA2137142C/en not_active Expired - Lifetime
- 1993-06-02 AT AT93915201T patent/ATE237684T1/de not_active IP Right Cessation
- 1993-06-02 DE DE69332893T patent/DE69332893T2/de not_active Expired - Lifetime
- 1993-06-02 WO PCT/US1993/005318 patent/WO1993025676A1/en not_active Ceased
- 1993-06-02 EP EP93915201A patent/EP0746611B1/de not_active Expired - Lifetime
- 1993-06-02 DK DK93915201T patent/DK0746611T3/da active
- 1993-06-02 ES ES93915201T patent/ES2194848T3/es not_active Expired - Lifetime
-
1994
- 1994-12-09 KR KR1019940704499A patent/KR0171205B1/ko not_active Expired - Lifetime
-
2003
- 2003-07-11 JP JP2003273656A patent/JP4148852B2/ja not_active Expired - Lifetime
-
2008
- 2008-05-12 JP JP2008124283A patent/JP4562786B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2137142C (en) | 2001-09-25 |
| JP2008271976A (ja) | 2008-11-13 |
| ES2194848T3 (es) | 2003-12-01 |
| JP3953094B2 (ja) | 2007-08-01 |
| JP4562786B2 (ja) | 2010-10-13 |
| JP2004041210A (ja) | 2004-02-12 |
| NZ253873A (en) | 1995-10-26 |
| DE69332893T2 (de) | 2003-12-24 |
| AU4527493A (en) | 1994-01-04 |
| CA2137142A1 (en) | 1993-12-23 |
| KR0171205B1 (en) | 1999-02-01 |
| DE69332893D1 (de) | 2003-05-22 |
| DK0746611T3 (da) | 2003-05-19 |
| JPH07508881A (ja) | 1995-10-05 |
| JP4148852B2 (ja) | 2008-09-10 |
| AU679448B2 (en) | 1997-07-03 |
| WO1993025676A1 (en) | 1993-12-23 |
| US6291205B1 (en) | 2001-09-18 |
| EP0746611A4 (de) | 1997-03-19 |
| EP0746611B1 (de) | 2003-04-16 |
| EP0746611A1 (de) | 1996-12-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |