ATE237684T1 - Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae) - Google Patents

Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae)

Info

Publication number
ATE237684T1
ATE237684T1 AT93915201T AT93915201T ATE237684T1 AT E237684 T1 ATE237684 T1 AT E237684T1 AT 93915201 T AT93915201 T AT 93915201T AT 93915201 T AT93915201 T AT 93915201T AT E237684 T1 ATE237684 T1 AT E237684T1
Authority
AT
Austria
Prior art keywords
proteins
recombinant
saccharomyces cerevisiae
pdi
strains
Prior art date
Application number
AT93915201T
Other languages
English (en)
Inventor
Michael F Tuite
Robert B Freedman
Loren D Schultz
Ronald W Ellis
Henry Z Markus
Donna L Montgomery
Original Assignee
Merck & Co Inc
Univ Kent Canterbury
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc, Univ Kent Canterbury filed Critical Merck & Co Inc
Application granted granted Critical
Publication of ATE237684T1 publication Critical patent/ATE237684T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT93915201T 1992-06-12 1993-06-02 Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae) ATE237684T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/901,713 US6291205B1 (en) 1992-06-12 1992-06-12 Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
PCT/US1993/005318 WO1993025676A1 (en) 1992-06-12 1993-06-02 METHOD FOR INCREASING PRODUCTION OF DISULFIDE BONDED RECOMBINANT PROTEINS BY $i(SACCHAROMYCES CEREVISIAE)

Publications (1)

Publication Number Publication Date
ATE237684T1 true ATE237684T1 (de) 2003-05-15

Family

ID=25414688

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93915201T ATE237684T1 (de) 1992-06-12 1993-06-02 Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae)

Country Status (12)

Country Link
US (1) US6291205B1 (de)
EP (1) EP0746611B1 (de)
JP (3) JP3953094B2 (de)
KR (1) KR0171205B1 (de)
AT (1) ATE237684T1 (de)
AU (1) AU679448B2 (de)
CA (1) CA2137142C (de)
DE (1) DE69332893T2 (de)
DK (1) DK0746611T3 (de)
ES (1) ES2194848T3 (de)
NZ (1) NZ253873A (de)
WO (1) WO1993025676A1 (de)

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ZA931601B (en) * 1992-03-06 1993-10-05 Squibb & Sons Inc Microsomal triglyceride transfer protein
DK76893D0 (de) * 1993-06-28 1993-06-28 Novo Nordisk As
EP0635574B1 (de) * 1993-07-23 2003-04-23 Dsm N.V. Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
AU2660397A (en) * 1996-04-05 1997-10-29 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
DK0828004T3 (da) * 1996-09-04 2007-03-19 Suntory Ltd Proteindisulfidisomerase-gen afledt af stamme af methylotrof gær
GB9703406D0 (en) 1997-02-19 1997-04-09 Chiron Spa Expression of heterologous proteins
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
US6521421B1 (en) 1997-07-16 2003-02-18 Genencor International, Inc. Expression vectors encoding Bacillus subtilis disulfide bond isomerase and methods of secreting proteins in gram-positive microorganisms using the same
DE69831571T2 (de) * 1997-07-16 2006-05-04 Genencor International, Inc., Palo Alto Erhöhung des produktions von proteinen in gram-positive mikroorganismen
EP1270730A4 (de) * 2000-03-14 2003-05-14 Itoham Foods Inc Verfahren zurherstellung eines polypeptids, welches eine disulfidbindung enthält
WO2003062375A2 (en) * 2001-12-19 2003-07-31 Genentech, Inc. Stabilizing polypeptides which have been exposed to urea
US20040203132A1 (en) 2003-02-28 2004-10-14 Cognetix, Inc. Conus protein disulfide isomerase
US7244616B2 (en) 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
WO2006067511A1 (en) 2004-12-23 2006-06-29 Novozymes Delta Limited Gene expression technique
GB0329681D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
GB0329722D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
US9057061B2 (en) 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
US20110034396A1 (en) * 2005-09-28 2011-02-10 Biovascular, Inc. Methods and compositions for inhibiting cell migration and treatment of inflammatory conditions
CA2623565A1 (en) * 2005-09-28 2007-04-05 Biovascular, Inc. Methods and compositions for blocking platelet and cell adhesion, cell migration and inflammation
JP2013009685A (ja) * 2005-11-24 2013-01-17 National Institute Of Advanced Industrial Science & Technology 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系
US8298787B2 (en) * 2006-06-20 2012-10-30 Mogam Biotechnology Research Institute Methods for enhancing a secretion efficiency of recombinant foreign protein in yeast expression system
FR2919063B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage du leucocyte elastase inhibitor pour le diagnostic in vitro du cancer colorectal.
ES2565512T3 (es) * 2007-07-19 2016-04-05 bioMérieux Procedimiento de ensayo de la proteína de unión a ácidos grasos del hígado, de ACE y de CA19-9 para el diagnóstico in vitro del cáncer colorrectal
FR2919061B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de la plastine-i pour le diagnostic in vitro du cancer colorectal.
FR2919060B1 (fr) 2007-07-19 2012-11-30 Biomerieux Sa Procede de dosage de l'ezrine pour le diagnostic in vitro du cancer colorectal.
FR2919065B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de l'apolipoproteine ai pour le diagnostic in vitro du cancer colorectal
FR2919062B1 (fr) * 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de l'aminoacylase 1 pour le diagnostic in vitro du cancer colorectal.
FR2919064B1 (fr) * 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de l'apolipoproteine all pour le diagnostic in vitro du cancer colorectal
JP5288379B2 (ja) † 2007-10-31 2013-09-11 独立行政法人産業技術総合研究所 タンパク質の高分泌生産方法
JP2011514157A (ja) * 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
FR2933773B1 (fr) 2008-07-10 2013-02-15 Biomerieux Sa Procede de dosage de la proteine disulfide isomerase pour le diagnostic in vitro du cancer colorectal
EP2258854A1 (de) 2009-05-20 2010-12-08 FH Campus Wien Eukaryotische Wirtszelle mit einem Ausdrucksverstärker
EP2565262A1 (de) * 2011-08-31 2013-03-06 VTU Holding GmbH Protein-Expression
CA2903739A1 (en) 2013-03-06 2014-09-12 Glaxosmithkline Llc Host cells and methods of use
SG10201809152QA (en) 2014-04-17 2018-11-29 Boehringer Ingelheim Rcv Gmbh Recombinant host cell for expressing proteins of interest
AU2015248815B2 (en) 2014-04-17 2021-07-08 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell engineered to overexpress helper proteins
IL282875B2 (en) 2018-11-08 2026-05-01 Sutro Biopharma Inc E. coli strains with oxygenated cytoplasm
US12351850B2 (en) 2020-04-30 2025-07-08 Sutro Biopharma, Inc. Methods of producing full-length antibodies using E. coli

Family Cites Families (6)

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Publication number Priority date Publication date Assignee Title
WO1988005816A1 (fr) * 1987-01-28 1988-08-11 Takeda Chemical Industries, Ltd. Polypeptide
IL86455A0 (en) 1987-06-01 1988-11-15 Takeda Chemical Industries Ltd Polypeptide and production thereof
JPH0638771A (ja) * 1990-10-31 1994-02-15 Tonen Corp ヒトプロテインジスルフィドイソメラーゼ遺伝子の発現方法および該遺伝子との共発現によるポリペプチドの製造方法
JPH05317037A (ja) * 1991-10-30 1993-12-03 Tonen Corp 酵母ｅｒｄ２遺伝子とそのリガンドとしての小胞体局在蛋白質をコードする遺伝子とを含む共発現系及びそれを利用する有用ポリペプチドの製造法
EP0509841A3 (en) * 1991-04-18 1993-08-18 Tonen Corporation Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen

Also Published As

Publication number Publication date
DE69332893D1 (de) 2003-05-22
ES2194848T3 (es) 2003-12-01
JP3953094B2 (ja) 2007-08-01
JPH07508881A (ja) 1995-10-05
EP0746611B1 (de) 2003-04-16
KR0171205B1 (en) 1999-02-01
JP2004041210A (ja) 2004-02-12
DK0746611T3 (da) 2003-05-19
EP0746611A1 (de) 1996-12-11
JP4562786B2 (ja) 2010-10-13
WO1993025676A1 (en) 1993-12-23
AU679448B2 (en) 1997-07-03
US6291205B1 (en) 2001-09-18
CA2137142C (en) 2001-09-25
AU4527493A (en) 1994-01-04
JP2008271976A (ja) 2008-11-13
CA2137142A1 (en) 1993-12-23
JP4148852B2 (ja) 2008-09-10
DE69332893T2 (de) 2003-12-24
NZ253873A (en) 1995-10-26
EP0746611A4 (de) 1997-03-19

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