ES2194848T3 - Procedimiento para aumentar la produccion de proteinas recombinantes con enlaces disulfuro por saccharomyces cerevisiae. - Google Patents

Procedimiento para aumentar la produccion de proteinas recombinantes con enlaces disulfuro por saccharomyces cerevisiae.

Info

Publication number
ES2194848T3
ES2194848T3 ES93915201T ES93915201T ES2194848T3 ES 2194848 T3 ES2194848 T3 ES 2194848T3 ES 93915201 T ES93915201 T ES 93915201T ES 93915201 T ES93915201 T ES 93915201T ES 2194848 T3 ES2194848 T3 ES 2194848T3
Authority
ES
Spain
Prior art keywords
proteins
production
increase
disulfure
united
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93915201T
Other languages
English (en)
Inventor
Michael F Tuite
Robert B Freedman
Loren D Schultz
Ronald W Ellis
Henry Z Markus
Donna L Montgomery
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Kent at Canterbury
Merck and Co Inc
Original Assignee
University of Kent at Canterbury
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Kent at Canterbury, Merck and Co Inc filed Critical University of Kent at Canterbury
Application granted granted Critical
Publication of ES2194848T3 publication Critical patent/ES2194848T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE REVELA UN PROCESO PARA INCREMENTAR LA PRODUCCION DE PROTEINAS RECOMBINANTES UNIDAS A DISULFURO PRODUCIDAS POR LEVADURA, ESPECIALMENTE PROTEINAS SECRETADAS RECOMBINANTES .LA ISOMERASA DE DISULFURO DE LAS PROTEINAS DE ENZIMAS (PDI) CATALIZA LA FORMACION DE ENLACES DE DISULFURO EN PROTEINAS SECRETORAS Y EN PROTEINAS DE LA SUPERFICIE CELULAR. DESVELAMOS LA CONSTRUCCION DE CEPAS RECOMBINANTES DE LA LEVADURA SCCHAROMYCES CEREVISIAE QUE PRODUCE CON EXCESO PDI HUMANO O LEVADURA PDI DE MANERA REGULADA. ESTAS CAPAS MUESTRAN UNA SECRECION ENORMEMENTE INCREMENTADA DE PROTEINAS UNIDAS A DISULFURO DE SIGNIFICACION TERAPEUTICA POTENCIAL. ESTAS CAPAS TIENEN EL POTENCIAL PARA INCREMENTAR LA PRODUCCION DE DIVERSAS PROTEINAS UNIDAS A DISULFURO.
ES93915201T 1992-06-12 1993-06-02 Procedimiento para aumentar la produccion de proteinas recombinantes con enlaces disulfuro por saccharomyces cerevisiae. Expired - Lifetime ES2194848T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/901,713 US6291205B1 (en) 1992-06-12 1992-06-12 Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae

Publications (1)

Publication Number Publication Date
ES2194848T3 true ES2194848T3 (es) 2003-12-01

Family

ID=25414688

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93915201T Expired - Lifetime ES2194848T3 (es) 1992-06-12 1993-06-02 Procedimiento para aumentar la produccion de proteinas recombinantes con enlaces disulfuro por saccharomyces cerevisiae.

Country Status (12)

Country Link
US (1) US6291205B1 (es)
EP (1) EP0746611B1 (es)
JP (3) JP3953094B2 (es)
KR (1) KR0171205B1 (es)
AT (1) ATE237684T1 (es)
AU (1) AU679448B2 (es)
CA (1) CA2137142C (es)
DE (1) DE69332893T2 (es)
DK (1) DK0746611T3 (es)
ES (1) ES2194848T3 (es)
NZ (1) NZ253873A (es)
WO (1) WO1993025676A1 (es)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU218419B (hu) * 1992-03-06 2000-08-28 E.R. Squibb And Sons, Inc. Mikroszomális triglicerid transzfer protein (MTP) nagy molekulatömegű alegységének rekombináns úton történő előállítására és a protein és inhibitorainak kimutatására szolgáló eljárások
DK76893D0 (es) 1993-06-28 1993-06-28 Novo Nordisk As
DE69432543T2 (de) * 1993-07-23 2003-12-24 Dsm N.V., Te Heerlen Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
AU2660397A (en) * 1996-04-05 1997-10-29 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
ATE346941T1 (de) * 1996-09-04 2006-12-15 Suntory Ltd Protein-disulfidisomerase-gen eines methylotrophen hefestammes
GB9703406D0 (en) 1997-02-19 1997-04-09 Chiron Spa Expression of heterologous proteins
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
US6521421B1 (en) 1997-07-16 2003-02-18 Genencor International, Inc. Expression vectors encoding Bacillus subtilis disulfide bond isomerase and methods of secreting proteins in gram-positive microorganisms using the same
DK1571217T3 (da) * 1997-07-16 2009-07-06 Genencor Int Ögende produktion af proteiner i gram-positive mikroorganismer
EP1270730A4 (en) * 2000-03-14 2003-05-14 Itoham Foods Inc METHOD FOR PRODUCING A POLYPEPTIDE THAT CONTAINS A DISULFIDE BINDING
US7452539B2 (en) * 2001-12-19 2008-11-18 Genentech, Inc. Stabilizing polypeptides which have been exposed to urea
US20040203132A1 (en) 2003-02-28 2004-10-14 Cognetix, Inc. Conus protein disulfide isomerase
US7244616B2 (en) 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
GB0329722D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
WO2006067511A1 (en) 2004-12-23 2006-06-29 Novozymes Delta Limited Gene expression technique
GB0329681D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
US9057061B2 (en) * 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
US20110034396A1 (en) * 2005-09-28 2011-02-10 Biovascular, Inc. Methods and compositions for inhibiting cell migration and treatment of inflammatory conditions
CA2623565A1 (en) * 2005-09-28 2007-04-05 Biovascular, Inc. Methods and compositions for blocking platelet and cell adhesion, cell migration and inflammation
JP2013009685A (ja) * 2005-11-24 2013-01-17 National Institute Of Advanced Industrial Science & Technology 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系
US8298787B2 (en) * 2006-06-20 2012-10-30 Mogam Biotechnology Research Institute Methods for enhancing a secretion efficiency of recombinant foreign protein in yeast expression system
FR2919061B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de la plastine-i pour le diagnostic in vitro du cancer colorectal.
JP5715817B2 (ja) * 2007-07-19 2015-05-13 ビオメリューＢｉｏｍｅｒｉｅｕｘ 結腸直腸癌のインビトロ診断のための肝臓脂肪酸結合タンパク質、ｃｅａおよびｃａ１９−９のアッセイのための方法
FR2919063B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage du leucocyte elastase inhibitor pour le diagnostic in vitro du cancer colorectal.
FR2919064B1 (fr) * 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de l'apolipoproteine all pour le diagnostic in vitro du cancer colorectal
FR2919060B1 (fr) * 2007-07-19 2012-11-30 Biomerieux Sa Procede de dosage de l'ezrine pour le diagnostic in vitro du cancer colorectal.
FR2919062B1 (fr) * 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de l'aminoacylase 1 pour le diagnostic in vitro du cancer colorectal.
FR2919065B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de l'apolipoproteine ai pour le diagnostic in vitro du cancer colorectal
ES2537806T5 (es) † 2007-10-31 2018-10-09 Daiichi Sankyo Company, Limited Método para la secreción y producción de alto nivel de proteína
US20100311122A1 (en) * 2008-02-20 2010-12-09 Glycofi, Inc Vectors and yeast strains for protein production
FR2933773B1 (fr) 2008-07-10 2013-02-15 Biomerieux Sa Procede de dosage de la proteine disulfide isomerase pour le diagnostic in vitro du cancer colorectal
EP2258854A1 (en) 2009-05-20 2010-12-08 FH Campus Wien Eukaryotic host cell comprising an expression enhancer
EP2565262A1 (en) 2011-08-31 2013-03-06 VTU Holding GmbH Protein expression
EP2964666B1 (en) 2013-03-06 2020-11-18 GlaxoSmithKline LLC Host cells and methods of use
CN106661541B (zh) 2014-04-17 2021-09-03 贝林格尔·英格海姆Rcv两合公司 被工程化为过表达辅助蛋白的重组宿主细胞
AU2015248807B2 (en) 2014-04-17 2021-07-22 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell for expressing proteins of interest
ES2944837T3 (es) 2018-11-08 2023-06-26 Sutro Biopharma Inc Cepas de E. coli con citoplasma oxidativo
US12351850B2 (en) 2020-04-30 2025-07-08 Sutro Biopharma, Inc. Methods of producing full-length antibodies using E. coli

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988005816A1 (fr) * 1987-01-28 1988-08-11 Takeda Chemical Industries, Ltd. Polypeptide
IL86455A0 (en) 1987-06-01 1988-11-15 Takeda Chemical Industries Ltd Polypeptide and production thereof
JPH0638771A (ja) * 1990-10-31 1994-02-15 Tonen Corp ヒトプロテインジスルフィドイソメラーゼ遺伝子の発現方法および該遺伝子との共発現によるポリペプチドの製造方法
EP0509841A3 (en) * 1991-04-18 1993-08-18 Tonen Corporation Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system
JPH05317037A (ja) * 1991-10-30 1993-12-03 Tonen Corp 酵母ｅｒｄ２遺伝子とそのリガンドとしての小胞体局在蛋白質をコードする遺伝子とを含む共発現系及びそれを利用する有用ポリペプチドの製造法
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen

Also Published As

Publication number Publication date
ATE237684T1 (de) 2003-05-15
JP3953094B2 (ja) 2007-08-01
AU4527493A (en) 1994-01-04
DE69332893T2 (de) 2003-12-24
EP0746611A1 (en) 1996-12-11
DE69332893D1 (de) 2003-05-22
EP0746611A4 (en) 1997-03-19
JP4562786B2 (ja) 2010-10-13
CA2137142A1 (en) 1993-12-23
JP2004041210A (ja) 2004-02-12
AU679448B2 (en) 1997-07-03
CA2137142C (en) 2001-09-25
WO1993025676A1 (en) 1993-12-23
KR0171205B1 (en) 1999-02-01
NZ253873A (en) 1995-10-26
JP4148852B2 (ja) 2008-09-10
JPH07508881A (ja) 1995-10-05
EP0746611B1 (en) 2003-04-16
US6291205B1 (en) 2001-09-18
DK0746611T3 (da) 2003-05-19
JP2008271976A (ja) 2008-11-13

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