ATE247175T1 - Methode zur eliminierung von sequenzierartefakten - Google Patents

Methode zur eliminierung von sequenzierartefakten

Info

Publication number: ATE247175T1
Authority: AT; Austria
Prior art keywords: dna sequencing; provides; sequencing artifacts; relates; methods
Prior art date: 1994-01-31

Application number

AT95909385T

Other languages

English (en)

Inventor

Michael Chamberlin

Daniel Mytelka

Original Assignee

Univ California

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1994-01-31

Filing date

1995-01-30

Publication date

2003-08-15

1995-01-30 Application filed by Univ California filed Critical Univ California

2003-08-15 Application granted granted Critical

2003-08-15 Publication of ATE247175T1 publication Critical patent/ATE247175T1/de

Links

238000000034 method Methods 0.000 title abstract 4
238000012163 sequencing technique Methods 0.000 title 1
238000001712 DNA sequencing Methods 0.000 abstract 3
KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 abstract 2
238000003752 polymerase chain reaction Methods 0.000 abstract 2
102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
FFDGPVCHZBVARC-UHFFFAOYSA-N N,N-dimethylglycine Chemical compound CN(C)CC(O)=O FFDGPVCHZBVARC-UHFFFAOYSA-N 0.000 abstract 1
229960003237 betaine Drugs 0.000 abstract 1
150000001875 compounds Chemical class 0.000 abstract 1
108700003601 dimethylglycine Proteins 0.000 abstract 1
230000008030 elimination Effects 0.000 abstract 1
238000003379 elimination reaction Methods 0.000 abstract 1
-1 nitrogen-containing organic compounds Chemical class 0.000 abstract 1
UYPYRKYUKCHHIB-UHFFFAOYSA-N trimethylamine N-oxide Chemical compound C[N+](C)(C)[O-] UYPYRKYUKCHHIB-UHFFFAOYSA-N 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Communication Control (AREA)
Control Of Stepping Motors (AREA)

AT95909385T 1994-01-31 1995-01-30 Methode zur eliminierung von sequenzierartefakten ATE247175T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US18924094A	1994-01-31	1994-01-31
PCT/US1995/001200 WO1995020682A1 (en)	1994-01-31	1995-01-30	Methods for the elimination of dna sequencing artifacts

Publications (1)

Publication Number	Publication Date
ATE247175T1 true ATE247175T1 (de)	2003-08-15

Family

ID=22696533

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT95909385T ATE247175T1 (de)	1994-01-31	1995-01-30	Methode zur eliminierung von sequenzierartefakten

Country Status (6)

Country	Link
EP (1)	EP0742838B1 (de)
JP (1)	JP3616394B2 (de)
AT (1)	ATE247175T1 (de)
AU (1)	AU694479B2 (de)
DE (1)	DE69531487T2 (de)
WO (1)	WO1995020682A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5545539A (en) *	1994-10-18	1996-08-13	Genzyme Corporation	Method for nucleotide sequence amplification
FR2733515B1 (fr) *	1995-04-27	1997-09-05	Cis Bio Int	Tampon, procede et methode d'evaluation des conditions optimales, d'amplification de sequences cibles d'acide nucleique, et leurs applications
ATE196510T1 (de) *	1996-05-23	2000-10-15	Qiagen Gmbh	Verwendung eines osmolyten zur verringerung oder aufhebung von nichtkovalenten bindungen biologischer moleküle an inerten oberflächen
US6150094A (en) *	1996-05-23	2000-11-21	Qiagen Gmbh	Use of an osmolyte for reducing or abolishing no-covalent interactions of biological molecules to inert surfaces
US6013488A (en) *	1996-07-25	2000-01-11	The Institute Of Physical And Chemical Research	Method for reverse transcription
US6291164B1 (en)	1996-11-22	2001-09-18	Invitrogen Corporation	Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
WO1998059075A1 (en)	1997-06-25	1998-12-30	Life Technologies, Inc.	Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
IL123256A0 (en)	1998-02-10	1998-09-24	Yeda Res & Dev	Methods for dna amplification and sequencing
US6787305B1 (en) *	1998-03-13	2004-09-07	Invitrogen Corporation	Compositions and methods for enhanced synthesis of nucleic acid molecules
US6183998B1 (en) *	1998-05-29	2001-02-06	Qiagen Gmbh Max-Volmer-Strasse 4	Method for reversible modification of thermostable enzymes
ES2296419T3 (es)	1998-11-12	2008-04-16	Invitrogen Corporation	Reactivos de transfeccion.
US6780588B2 (en) *	2001-05-07	2004-08-24	Applera Corporation	Methods for the reduction of stutter in microsatellite amplification
DE60231901D1 (de)	2001-11-14	2009-05-20	Toyo Boseki	Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
GB2420560A (en) *	2004-11-30	2006-05-31	Bioline Ltd	A method for increasing the specificity of enzymatic DNA synthesis
JP4761265B2 (ja) *	2007-10-17	2011-08-31	学校法人甲南学園	核酸合成を促進する化合物を含む組成物およびその利用、並びに当該化合物の製造方法
EP2894226B1 (de) *	2009-01-08	2021-04-14	Bio-Rad Laboratories, Inc.	Verfahren und zusammensetzung zur verbesserung der wirksamkeit bei einer nukleinsäureverstärkungsreaktion
CN106572974B (zh)	2014-07-15	2021-04-23	生命技术公司	用于将分子有效递送到细胞的具有脂质聚集体的组合物和方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE4411588C1 (de) *	1994-03-30	1995-09-28	Deutsches Rheuma Forschungszen	Puffer für DNA- und RNA-Polymerase-Reaktionen
US5545539A (en) *	1994-10-18	1996-08-13	Genzyme Corporation	Method for nucleotide sequence amplification

1995
- 1995-01-30 WO PCT/US1995/001200 patent/WO1995020682A1/en not_active Ceased
- 1995-01-30 JP JP52022495A patent/JP3616394B2/ja not_active Expired - Lifetime
- 1995-01-30 AU AU17365/95A patent/AU694479B2/en not_active Expired
- 1995-01-30 AT AT95909385T patent/ATE247175T1/de active
- 1995-01-30 EP EP95909385A patent/EP0742838B1/de not_active Expired - Lifetime
- 1995-01-30 DE DE69531487T patent/DE69531487T2/de not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DE69531487T2 (de)	2004-06-17
EP0742838A4 (de)	2000-01-05
JP3616394B2 (ja)	2005-02-02
EP0742838A1 (de)	1996-11-20
EP0742838B1 (de)	2003-08-13
AU1736595A (en)	1995-08-15
AU694479B2 (en)	1998-07-23
WO1995020682A1 (en)	1995-08-03
DE69531487D1 (de)	2003-09-18
JPH09511133A (ja)	1997-11-11

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2004-05-15

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