ATE250125T1 - Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen - Google Patents

Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen

Info

Publication number
ATE250125T1
ATE250125T1 AT99971031T AT99971031T ATE250125T1 AT E250125 T1 ATE250125 T1 AT E250125T1 AT 99971031 T AT99971031 T AT 99971031T AT 99971031 T AT99971031 T AT 99971031T AT E250125 T1 ATE250125 T1 AT E250125T1
Authority
AT
Austria
Prior art keywords
nucleic acid
bacterial cells
polypeptides
acid encoding
heterologue
Prior art date
Application number
AT99971031T
Other languages
English (en)
Inventor
Woon-Lam Susan Leung
James R Swartz
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE250125T1 publication Critical patent/ATE250125T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2839—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
    • C07K16/2845—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily against integrin beta2-subunit-containing molecules, e.g. CD11, CD18
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/54—F(ab')2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10211—Podoviridae
    • C12N2795/10222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Epoxy Compounds (AREA)
AT99971031T 1998-10-28 1999-10-21 Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen ATE250125T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10605298P 1998-10-28 1998-10-28
PCT/US1999/024696 WO2000024873A1 (en) 1998-10-28 1999-10-21 Process for recovering heterologous polypeptides from bacterial cells

Publications (1)

Publication Number Publication Date
ATE250125T1 true ATE250125T1 (de) 2003-10-15

Family

ID=22309219

Family Applications (2)

Application Number Title Priority Date Filing Date
AT99971031T ATE250125T1 (de) 1998-10-28 1999-10-21 Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen
AT03006278T ATE415469T1 (de) 1998-10-28 1999-10-21 Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT03006278T ATE415469T1 (de) 1998-10-28 1999-10-21 Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen

Country Status (13)

Country Link
US (2) US6180367B1 (de)
EP (2) EP1124941B1 (de)
JP (1) JP3711326B2 (de)
AT (2) ATE250125T1 (de)
AU (1) AU752429B2 (de)
CA (1) CA2346797C (de)
CY (1) CY1109847T1 (de)
DE (2) DE69939991D1 (de)
DK (2) DK1323820T3 (de)
ES (2) ES2207340T3 (de)
IL (2) IL142269A0 (de)
PT (2) PT1124941E (de)
WO (1) WO2000024873A1 (de)

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WO2001050526A1 (en) * 1999-12-30 2001-07-12 Intel Corporation Optimized driver layout for integrated circuits with staggered bond pads
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US20060156998A1 (en) * 2005-01-19 2006-07-20 Shawn Bridy Protective collar for animals
BRPI0621124A2 (pt) 2005-12-22 2011-11-29 Genentech Inc métodos para recuperação de uma proteìna de ligação à heparina
AR062069A1 (es) 2006-07-14 2008-10-15 Genentech Inc Replegado de proteinas recombinantes
EP2431470B1 (de) * 2006-11-30 2017-11-29 Novozymes A/S Rekombinante wirtszellen der dnase-expression
EP2468869B1 (de) * 2007-01-31 2015-03-18 Pfenex Inc. Bakterielle Leader-Sequenzen für erhöhte Expression
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9017966B2 (en) * 2007-05-23 2015-04-28 Nature Technology Corporation E. coli plasmid DNA production
US20110020830A1 (en) * 2008-03-31 2011-01-27 Schneider Jane C Design for rapidly cloning one or more polypeptide chains into an expression system
ES2370947B1 (es) * 2009-12-23 2012-11-02 Consejo Superior De Investigaciones Científicas (Csic) Sistema de autolisis celular para el procesado de la biomasa bacteriana en la produccion de polihidroxialcanoatos en pseudomonas putida kt2440
KR101222889B1 (ko) 2010-02-25 2013-01-17 고려대학교 산학협력단 이중프로모터를 포함하는 발현 시스템 및 그 시스템을 이용한 유전자 과다 발현 방법
WO2013147043A1 (ja) * 2012-03-29 2013-10-03 株式会社シノテスト 核酸の検出又は定量方法及び検出又は定量用試薬キット
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PL2968470T3 (pl) 2013-03-12 2021-05-31 The General Hospital Corporation Zmodyfikowane białka stanowiące substancję hamującą rozwój przewodów müllera (mis) i ich zastosowania do leczenia chorób
ES2912283T3 (es) 2013-12-11 2022-05-25 Massachusetts Gen Hospital Uso de proteínas de tipo sustancia inhibidora mülleriana (SIM) para la anticoncepción y conservación de la reserva ovárica
JP6803328B2 (ja) 2014-08-28 2021-01-06 シンセティック バイオロジクス，インコーポレイテッド 大腸菌ベースでのベータラクタマーゼ生産
EP3436610A1 (de) * 2016-03-29 2019-02-06 Geltor, Inc. Expression von proteinen in gramnegativen bakterien mit verhältnis von periplasmatischem volumen zu zytoplasmatischem volumen zwischen 0,5:1 und 10:1
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Also Published As

Publication number Publication date
US6258560B1 (en) 2001-07-10
CA2346797A1 (en) 2000-05-04
ATE415469T1 (de) 2008-12-15
AU1219500A (en) 2000-05-15
WO2000024873A1 (en) 2000-05-04
DE69911450T2 (de) 2004-07-22
CY1109847T1 (el) 2014-09-10
WO2000024873A9 (en) 2000-11-02
EP1124941B1 (de) 2003-09-17
CA2346797C (en) 2008-09-23
DK1124941T3 (da) 2004-02-02
EP1323820B1 (de) 2008-11-26
EP1323820A2 (de) 2003-07-02
IL142269A (en) 2010-12-30
DE69911450D1 (de) 2003-10-23
EP1323820A3 (de) 2003-07-09
EP1124941A1 (de) 2001-08-22
PT1124941E (pt) 2004-02-27
DK1323820T3 (da) 2009-03-16
ES2207340T3 (es) 2004-05-16
ES2318070T3 (es) 2009-05-01
AU752429B2 (en) 2002-09-19
DE69939991D1 (de) 2009-01-08
US6180367B1 (en) 2001-01-30
JP3711326B2 (ja) 2005-11-02
JP2002528071A (ja) 2002-09-03
PT1323820E (pt) 2009-03-05
IL142269A0 (en) 2002-03-10

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