ATE415469T1 - Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen - Google Patents
Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellenInfo
- Publication number
- ATE415469T1 ATE415469T1 AT03006278T AT03006278T ATE415469T1 AT E415469 T1 ATE415469 T1 AT E415469T1 AT 03006278 T AT03006278 T AT 03006278T AT 03006278 T AT03006278 T AT 03006278T AT E415469 T1 ATE415469 T1 AT E415469T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- bacterial cells
- polypeptides
- acid encoding
- heterologue
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2839—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
- C07K16/2845—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily against integrin beta2-subunit-containing molecules, e.g. CD11, CD18
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/54—F(ab')2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10211—Podoviridae
- C12N2795/10222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10311—Siphoviridae
- C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Diabetes (AREA)
- Mycology (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Epoxy Compounds (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10605298P | 1998-10-28 | 1998-10-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE415469T1 true ATE415469T1 (de) | 2008-12-15 |
Family
ID=22309219
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99971031T ATE250125T1 (de) | 1998-10-28 | 1999-10-21 | Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen |
| AT03006278T ATE415469T1 (de) | 1998-10-28 | 1999-10-21 | Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99971031T ATE250125T1 (de) | 1998-10-28 | 1999-10-21 | Verfahren zur gewinnung von heterologen polypeptiden aus bakterienzellen |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US6180367B1 (de) |
| EP (2) | EP1323820B1 (de) |
| JP (1) | JP3711326B2 (de) |
| AT (2) | ATE250125T1 (de) |
| AU (1) | AU752429B2 (de) |
| CA (1) | CA2346797C (de) |
| CY (1) | CY1109847T1 (de) |
| DE (2) | DE69911450T2 (de) |
| DK (2) | DK1323820T3 (de) |
| ES (2) | ES2318070T3 (de) |
| IL (2) | IL142269A0 (de) |
| PT (2) | PT1124941E (de) |
| WO (1) | WO2000024873A1 (de) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4298165B2 (ja) * | 1998-03-30 | 2009-07-15 | メタボリックス，インコーポレイテッド | 生体適合材料の製造のための微生物株およびプロセス |
| US7858339B1 (en) | 1998-10-28 | 2010-12-28 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US6784558B2 (en) * | 1999-12-30 | 2004-08-31 | Intel Corporation | Semiconductor device inlcluding optimized driver layout for integrated circuit with staggered bond pads |
| AU4305501A (en) * | 1999-12-30 | 2001-07-16 | Intel Corporation | Optimized driver layout for integrated circuits with staggered bond pads |
| EP1427744B1 (de) | 2001-08-27 | 2007-12-26 | Genentech, Inc. | System zur antikörperexpression und- synthese |
| US20030198682A1 (en) * | 2002-02-12 | 2003-10-23 | Gruber James V. | Composition and method for protecting labile active components during high temperature drying |
| US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
| US7195611B1 (en) * | 2002-12-31 | 2007-03-27 | Advanced Cardiovascular Systems, Inc. | Rapid exchange balloon catheter having a reinforced inner tubular member |
| WO2004081033A2 (en) * | 2003-03-11 | 2004-09-23 | National Institutes Of Health | Synthesis of proteins by cell-free protein expression |
| US7985564B2 (en) | 2003-11-21 | 2011-07-26 | Pfenex, Inc. | Expression systems with sec-system secretion |
| EP2412816B1 (de) | 2004-07-26 | 2014-12-03 | Pfenex Inc. | Verfahren zur verbesserten Proteinexpression durch Strain-Engineering |
| AU2005280356B2 (en) | 2004-08-16 | 2008-09-25 | Clague P. Hodgson | Improved strains of E. coli for plasmid DNA production |
| US20060156998A1 (en) * | 2005-01-19 | 2006-07-20 | Shawn Bridy | Protective collar for animals |
| NZ568809A (en) | 2005-12-22 | 2011-08-26 | Genentech Inc | Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents |
| AU2007272412B2 (en) | 2006-07-14 | 2013-11-07 | Genentech, Inc. | Refolding of recombinant proteins |
| EP2089524B1 (de) * | 2006-11-30 | 2012-10-10 | Novozymes A/S | Rekombinante wirtszellen der dnase-expression |
| EP2468869B1 (de) * | 2007-01-31 | 2015-03-18 | Pfenex Inc. | Bakterielle Leader-Sequenzen für erhöhte Expression |
| US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| EP2615172A1 (de) | 2007-04-27 | 2013-07-17 | Pfenex Inc. | Schnellscreeningverfahren für Bakterienwirte zur Identifizierung bestimmter Stämme mit verbessertem Ertrag und/oder verbesserter Qualität bei der Expression heterologer Proteine |
| EP2160459A2 (de) * | 2007-05-23 | 2010-03-10 | Nature Technology Corp. | Verbesserte e. coli-plasmid-dna-herstellung |
| US20110020830A1 (en) * | 2008-03-31 | 2011-01-27 | Schneider Jane C | Design for rapidly cloning one or more polypeptide chains into an expression system |
| ES2370947B1 (es) * | 2009-12-23 | 2012-11-02 | Consejo Superior De Investigaciones Científicas (Csic) | Sistema de autolisis celular para el procesado de la biomasa bacteriana en la produccion de polihidroxialcanoatos en pseudomonas putida kt2440 |
| KR101222889B1 (ko) | 2010-02-25 | 2013-01-17 | 고려대학교 산학협력단 | 이중프로모터를 포함하는 발현 시스템 및 그 시스템을 이용한 유전자 과다 발현 방법 |
| JP6402347B2 (ja) * | 2012-03-29 | 2018-10-10 | 株式会社シノテスト | 核酸の検出又は定量方法及び検出又は定量用試薬キット |
| GB201208367D0 (en) | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Biological product |
| BR112015022181A8 (pt) | 2013-03-12 | 2018-01-23 | Massachusetts Eye & Ear Infirmary | proteínas da substância de inibição mulleriana (mis) e usos das mesmas para o tratamento de doenças |
| CA2933335A1 (en) | 2013-12-11 | 2015-06-18 | The General Hospital Corporation | Use of mullerian inhibiting substance (mis) proteins for contraception and ovarian reserve preservation |
| EP3186379B1 (de) | 2014-08-28 | 2020-04-08 | Synthetic Biologics, Inc. | Auf e. coli basierende herstellung von beta-lactamase |
| KR20190012145A (ko) * | 2016-03-29 | 2019-02-08 | 젤터, 인코포레이티드 | 주변세포질 부피 대 세포질 부피의 비가 0.5:1 내지 10:1인 그람-음성 박테리아에서의 단백질의 발현 |
| US11180541B2 (en) | 2017-09-28 | 2021-11-23 | Geltor, Inc. | Recombinant collagen and elastin molecules and uses thereof |
| GB201814590D0 (en) * | 2018-09-07 | 2018-10-24 | Oxford Biomedica Ltd | Viral vector production system |
| JP2022024197A (ja) * | 2018-09-28 | 2022-02-09 | Ｓｐｉｂｅｒ株式会社 | 組換え細胞、組換え細胞の破砕方法及び目的タンパク質の製造方法 |
| GB2597416A (en) | 2019-04-12 | 2022-01-26 | Geltor Inc | Recombinant elastin and production thereof |
| EP4010500B1 (de) | 2019-08-05 | 2025-02-19 | Wacker Chemie AG | Bakterienstamm zur freisetzung eines rekombinanten proteins in einem fermentationsverfahren |
| US20250197909A1 (en) * | 2020-04-03 | 2025-06-19 | Novozymes A/S | Submerged fermentation process |
| EP3926039A1 (de) * | 2020-06-17 | 2021-12-22 | AB Enzymes GmbH | Verwendung einer nuklease zur verringerung der viskosität und/oder zur verhinderung eines viskositätsanstiegs einer fermentationsbrühe |
| US20240352432A1 (en) * | 2021-08-11 | 2024-10-24 | Duke University | Rapid expression and purification of thermostable proteins including taq polymerase |
| WO2024133316A1 (en) * | 2022-12-23 | 2024-06-27 | Thermo Fisher Scientific Baltics Uab | Closed system for expressing recombinant proteins using e. coli and methods for producing clarified cell cultures thereof |
| CN118420716B (zh) * | 2024-05-28 | 2025-10-31 | 青岛农业大学 | 一种多肽125、包含多肽125的生物材料及应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4595658A (en) | 1982-09-13 | 1986-06-17 | The Rockefeller University | Method for facilitating externalization of proteins synthesized in bacteria |
| US4637980A (en) * | 1983-08-09 | 1987-01-20 | Smithkline Beckman Corporation | Externalization of products of bacteria |
| EP0155189A3 (de) * | 1984-03-16 | 1987-09-16 | Genentech, Inc. | Expression eines die Zellwand degradierenden Proteins und für ein solches Protein kodierende DNS enthaltende Wirtszellen |
| DE3507648A1 (de) | 1985-03-05 | 1986-09-11 | Klöckner-Humboldt-Deutz AG, 5000 Köln | Vorrichtung und verfahren zum warmhalten von fluessigen metallschmelzen |
| JPH0640832B2 (ja) | 1985-05-10 | 1994-06-01 | 味の素株式会社 | インタ−ロイキン２ポリペプチドの回収方法 |
| SE8505027D0 (sv) * | 1985-10-24 | 1985-10-24 | Kabigen Ab | A lytic virulent phage, a hostcell containing same and a process for protein production |
| US5169772A (en) | 1988-06-06 | 1992-12-08 | Massachusetts Institute Of Technology | Large scale method for purification of high purity heparinase from flavobacterium heparinum |
| US5512456A (en) * | 1990-05-25 | 1996-04-30 | James Madison University | Method for the improved production and recovery of poly-β-hydroxybutyrate from transformed Escherichia coli |
| RU2043415C1 (ru) | 1992-02-18 | 1995-09-10 | Биотехнологическая компания "Биосервис" | ВЕКТОР PEL 5b, ПРЕДНАЗНАЧЕННЫЙ ДЛЯ ЭКСПРЕССИИ ЧУЖЕРОДНОЙ ДНК |
| RU2071501C1 (ru) | 1992-02-18 | 1997-01-10 | Биотехнологическая компания "Биосервис" | Вектор реl25а, предназначенный для экспрессии чужеродного днк |
| RU2071503C1 (ru) | 1992-02-18 | 1997-01-10 | Биотехнологическая компания "Биосервис" | ВЕКТОР pЕL5С, ПРЕДНАЗНАЧЕННЫЙ ДЛЯ ЭКСПРЕССИИ ЧУЖЕРОДНОЙ ДНК |
| AU5732694A (en) | 1992-12-01 | 1994-06-22 | Protein Design Labs, Inc. | Humanized antibodies reactive with cd18 |
-
1999
- 1999-10-21 PT PT99971031T patent/PT1124941E/pt unknown
- 1999-10-21 DK DK03006278T patent/DK1323820T3/da active
- 1999-10-21 JP JP2000578427A patent/JP3711326B2/ja not_active Expired - Lifetime
- 1999-10-21 ES ES03006278T patent/ES2318070T3/es not_active Expired - Lifetime
- 1999-10-21 PT PT03006278T patent/PT1323820E/pt unknown
- 1999-10-21 EP EP03006278A patent/EP1323820B1/de not_active Expired - Lifetime
- 1999-10-21 DE DE69911450T patent/DE69911450T2/de not_active Expired - Lifetime
- 1999-10-21 AT AT99971031T patent/ATE250125T1/de active
- 1999-10-21 ES ES99971031T patent/ES2207340T3/es not_active Expired - Lifetime
- 1999-10-21 US US09/422,712 patent/US6180367B1/en not_active Expired - Lifetime
- 1999-10-21 AT AT03006278T patent/ATE415469T1/de active
- 1999-10-21 DK DK99971031T patent/DK1124941T3/da active
- 1999-10-21 WO PCT/US1999/024696 patent/WO2000024873A1/en not_active Ceased
- 1999-10-21 CA CA002346797A patent/CA2346797C/en not_active Expired - Lifetime
- 1999-10-21 DE DE69939991T patent/DE69939991D1/de not_active Expired - Lifetime
- 1999-10-21 EP EP99971031A patent/EP1124941B1/de not_active Expired - Lifetime
- 1999-10-21 IL IL14226999A patent/IL142269A0/xx unknown
- 1999-10-21 AU AU12195/00A patent/AU752429B2/en not_active Expired
-
2000
- 2000-06-29 US US09/607,756 patent/US6258560B1/en not_active Expired - Lifetime
-
2001
- 2001-03-27 IL IL142269A patent/IL142269A/en not_active IP Right Cessation
-
2009
- 2009-02-18 CY CY20091100188T patent/CY1109847T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1323820A3 (de) | 2003-07-09 |
| CA2346797C (en) | 2008-09-23 |
| CA2346797A1 (en) | 2000-05-04 |
| IL142269A (en) | 2010-12-30 |
| AU1219500A (en) | 2000-05-15 |
| DE69939991D1 (de) | 2009-01-08 |
| PT1323820E (pt) | 2009-03-05 |
| JP3711326B2 (ja) | 2005-11-02 |
| ATE250125T1 (de) | 2003-10-15 |
| IL142269A0 (en) | 2002-03-10 |
| ES2207340T3 (es) | 2004-05-16 |
| ES2318070T3 (es) | 2009-05-01 |
| AU752429B2 (en) | 2002-09-19 |
| JP2002528071A (ja) | 2002-09-03 |
| EP1323820A2 (de) | 2003-07-02 |
| EP1124941A1 (de) | 2001-08-22 |
| WO2000024873A1 (en) | 2000-05-04 |
| PT1124941E (pt) | 2004-02-27 |
| EP1124941B1 (de) | 2003-09-17 |
| DE69911450T2 (de) | 2004-07-22 |
| US6180367B1 (en) | 2001-01-30 |
| CY1109847T1 (el) | 2014-09-10 |
| WO2000024873A9 (en) | 2000-11-02 |
| DK1323820T3 (da) | 2009-03-16 |
| US6258560B1 (en) | 2001-07-10 |
| DK1124941T3 (da) | 2004-02-02 |
| EP1323820B1 (de) | 2008-11-26 |
| DE69911450D1 (de) | 2003-10-23 |
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