ATE265539T1 - Verfahren zur isolierung von ccc plasmid dna - Google Patents

Verfahren zur isolierung von ccc plasmid dna

Info

Publication number
ATE265539T1
ATE265539T1 AT99926364T AT99926364T ATE265539T1 AT E265539 T1 ATE265539 T1 AT E265539T1 AT 99926364 T AT99926364 T AT 99926364T AT 99926364 T AT99926364 T AT 99926364T AT E265539 T1 ATE265539 T1 AT E265539T1
Authority
AT
Austria
Prior art keywords
plasmid dna
feed
isolation
batch
ccc plasmid
Prior art date
Application number
AT99926364T
Other languages
English (en)
Inventor
Torsten Schmidt
Karl Friehs
Erwin Flaschel
Martin Schleef
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE265539T1 publication Critical patent/ATE265539T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT99926364T 1998-05-25 1999-05-21 Verfahren zur isolierung von ccc plasmid dna ATE265539T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98109473 1998-05-25
PCT/EP1999/003522 WO1999061633A2 (en) 1998-05-25 1999-05-21 Method for the isolation of ccc plasmid dna

Publications (1)

Publication Number Publication Date
ATE265539T1 true ATE265539T1 (de) 2004-05-15

Family

ID=8231999

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99926364T ATE265539T1 (de) 1998-05-25 1999-05-21 Verfahren zur isolierung von ccc plasmid dna

Country Status (11)

Country Link
US (1) US6664078B1 (de)
EP (1) EP1144656B1 (de)
JP (1) JP2002516113A (de)
AT (1) ATE265539T1 (de)
AU (1) AU753138B2 (de)
CA (1) CA2329246A1 (de)
DE (1) DE69916892T2 (de)
DK (1) DK1144656T3 (de)
ES (1) ES2217763T3 (de)
PT (1) PT1144656E (de)
WO (1) WO1999061633A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10106493B4 (de) * 2001-02-13 2010-11-04 Plasmidfactory Gmbh & Co. Kg Verfahren zur Herstellung von Nukleinsäuren
CA2454701A1 (en) * 2001-07-23 2003-02-06 Dsm Ip Assets B.V. Nucleic acid sequences encoding enantioselective amidases
KR101229849B1 (ko) * 2004-03-25 2013-02-05 더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 쥬니어 유니버시티 소포제의 첨가에 의한 무세포 단백질 합성 시스템의 단백질발현 수율 개선 방법
EP1584680A1 (de) * 2004-04-08 2005-10-12 Boehringer Ingelheim Austria GmbH Fed-Batch Fermentationsverfahren zur Herstellung von Plasmid-DNA
US20050233365A1 (en) * 2004-04-08 2005-10-20 Boehringer Ingelheim Austria Gmbh Method for producing plasmid DNA on a manufacturing scale
AU2005277510B2 (en) * 2004-08-19 2009-09-17 Aldevron, L.L.C. Process for plasmid DNA fermentation
WO2007089873A2 (en) * 2006-02-01 2007-08-09 Nature Technology Corporation Fermentation process for continuous plasmid dna production
US20110269184A1 (en) 2009-07-16 2011-11-03 Boehringer Ingelheim Rcv Gmbh & Co. Kg Method for controlling plasmid copy number in e.coli
EP3655522A4 (de) 2017-07-21 2021-04-14 Conagen, Inc. Plasmidabhängigkeitssystem zur ansteuerung der gewünschten genexpression
CN111099740B (zh) * 2018-10-26 2022-06-07 中国石油化工股份有限公司 一种化能自养微生物培养过程的补料控制方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD239222A1 (de) * 1985-07-10 1986-09-17 Dresden Arzneimittel Verfahren zur herstellung von plasmid-dna vom cole1-typ
DD299380A7 (de) * 1990-01-19 1992-04-16 Institut F. Technologie Der Polymere,De Verfahren zur hochzelldichte-fermentation von escherichia coli in einem ruehrkesselfermentor
WO1996040905A1 (en) * 1995-06-07 1996-12-19 Vical Incorporated Optimized high-yield production of plasmid dna
US5955323A (en) 1996-08-01 1999-09-21 American Home Products Corporation Automated high-yield fermentation of plasmid DNA in Escherichia coli

Also Published As

Publication number Publication date
EP1144656B1 (de) 2004-04-28
DK1144656T3 (da) 2004-07-19
CA2329246A1 (en) 1999-12-02
WO1999061633A3 (en) 2001-10-11
AU4365899A (en) 1999-12-13
WO1999061633A2 (en) 1999-12-02
PT1144656E (pt) 2004-07-30
DE69916892T2 (de) 2005-01-20
US6664078B1 (en) 2003-12-16
AU753138B2 (en) 2002-10-10
JP2002516113A (ja) 2002-06-04
DE69916892D1 (de) 2004-06-03
ES2217763T3 (es) 2004-11-01
EP1144656A2 (de) 2001-10-17

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