ATE265545T1 - Ligationsassemblierung und detektion von polynucleotiden auf einem festträger - Google Patents

Ligationsassemblierung und detektion von polynucleotiden auf einem festträger

Info

Publication number
ATE265545T1
ATE265545T1 AT99945166T AT99945166T ATE265545T1 AT E265545 T1 ATE265545 T1 AT E265545T1 AT 99945166 T AT99945166 T AT 99945166T AT 99945166 T AT99945166 T AT 99945166T AT E265545 T1 ATE265545 T1 AT E265545T1
Authority
AT
Austria
Prior art keywords
polynucleotides
detection
solid support
solid
polynucleotide
Prior art date
Application number
AT99945166T
Other languages
English (en)
Inventor
Michael W Hunkapiller
Andrew C Hiatt
Original Assignee
Applera Corp
Andrew C Hiatt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp, Andrew C Hiatt filed Critical Applera Corp
Application granted granted Critical
Publication of ATE265545T1 publication Critical patent/ATE265545T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99945166T 1998-11-12 1999-08-24 Ligationsassemblierung und detektion von polynucleotiden auf einem festträger ATE265545T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/191,390 US5942609A (en) 1998-11-12 1998-11-12 Ligation assembly and detection of polynucleotides on solid-support
PCT/US1999/019282 WO2000029616A1 (en) 1998-11-12 1999-08-24 Ligation assembly and detection of polynucleotides on solid-support

Publications (1)

Publication Number Publication Date
ATE265545T1 true ATE265545T1 (de) 2004-05-15

Family

ID=22705298

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99945166T ATE265545T1 (de) 1998-11-12 1999-08-24 Ligationsassemblierung und detektion von polynucleotiden auf einem festträger

Country Status (8)

Country Link
US (1) US5942609A (de)
EP (1) EP1129215B1 (de)
JP (1) JP3544945B2 (de)
AT (1) ATE265545T1 (de)
AU (1) AU770217B2 (de)
CA (1) CA2353076A1 (de)
DE (1) DE69916877T2 (de)
WO (1) WO2000029616A1 (de)

Families Citing this family (167)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
ATE296310T1 (de) * 1999-03-08 2005-06-15 Metrigen Inc Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
US6593464B1 (en) * 1999-05-24 2003-07-15 Invitrogen Corporation Method for deblocking of labeled oligonucleotides
WO2001032845A2 (en) * 1999-11-05 2001-05-10 Applied Nanosystems B.V. Methods for the preparation of (lipo)peptide synthetases and (lipo)peptides produced therewith
US20020009728A1 (en) * 2000-01-18 2002-01-24 Quantum Dot Corporation Oligonucleotide-tagged semiconductor nanocrystals for microarray and fluorescence in situ hybridization
JP2001204463A (ja) * 2000-01-27 2001-07-31 Toyo Kohan Co Ltd ヌクレオチド固定用担体
US6579680B2 (en) * 2000-02-28 2003-06-17 Corning Incorporated Method for label-free detection of hybridized DNA targets
US20040009514A1 (en) * 2000-02-28 2004-01-15 Frutos Anthony G. Assembly for label-free detection of hybridized nucleic targets
WO2004061126A2 (en) * 2002-03-25 2004-07-22 Datascope Investment Corp. Nucleic acid hybridization assay using bridging sequence
US6368801B1 (en) 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
AU2001275091A1 (en) 2000-06-02 2001-12-17 Blue Heron Biotechnology, Inc. Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides
EP1205548A1 (de) * 2000-11-09 2002-05-15 Kabushiki Kaisha Toyota Chuo Kenkyusho Verfahren und Vorrichtung zur Festphasen-Ligation von Doppelsträngige DNA-Molekülen
JP4516273B2 (ja) * 2000-11-15 2010-08-04 ミナーヴァ・バイオテクノロジーズ・コーポレーション オリゴヌクレオチド識別子
GB0031093D0 (en) * 2000-12-20 2001-01-31 Birkbeck College Materials and methods for nucleic acid systhesis
DE10104025B4 (de) * 2001-01-31 2008-07-10 Qiagen North American Holdings, Inc. Verfahren zur Aufreinigung und anschließenden Amplifikation von Doppelstrang-DNA
US20030049619A1 (en) * 2001-03-21 2003-03-13 Simon Delagrave Methods for the synthesis of polynucleotides and combinatorial libraries of polynucleotides
US7164992B1 (en) 2001-03-22 2007-01-16 Blue Heron Biotechnology, Inc. Method and system for polynucleotide synthesis
EP1401850A1 (de) 2001-06-20 2004-03-31 Nuevolution A/S Nukleosidderivate zur herstellung einer verbindungsbibliothek
US6972173B2 (en) * 2002-03-14 2005-12-06 Intel Corporation Methods to increase nucleotide signals by raman scattering
US7238477B2 (en) * 2001-09-24 2007-07-03 Intel Corporation Methods to increase nucleotide signals by Raman scattering
US20040241701A1 (en) * 2001-11-07 2004-12-02 Vaughn Smider Method for generation of modular polynucleotides using solid supports
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US6696254B2 (en) * 2001-11-21 2004-02-24 Kimberly-Clark Worldwide, Inc. Detection and identification of enteric bacteria
US20030148284A1 (en) * 2001-12-17 2003-08-07 Vision Todd J. Solid phase detection of nucleic acid molecules
AU2003207448A1 (en) * 2002-01-04 2003-07-24 Board Of Regents, The University Of Texas System Proofreading, error deletion, and ligation method for synthesis of high-fidelity polynucleotide sequences
ATE431407T1 (de) * 2002-01-11 2009-05-15 Biospring Ges Fuer Biotechnolo Verfahren zur herstellung von dna
AU2003214031A1 (en) * 2002-03-15 2003-09-29 Nuevolution A/S An improved method for synthesising templated molecules
US10731151B2 (en) 2002-03-15 2020-08-04 Nuevolution A/S Method for synthesising templated molecules
US20030186301A1 (en) * 2002-03-25 2003-10-02 The Regents Of The University Of California Constructing user-defined, long DNA sequences
JP2005521419A (ja) * 2002-04-01 2005-07-21 ブルー ヘロン バイオテクノロジー インコーポレイテッド ポリヌクレオチド作製のための固相方法
US7482119B2 (en) * 2002-04-01 2009-01-27 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
EP1530581A4 (de) * 2002-06-12 2006-08-30 Datascope Investment Corp Polymere markermoleküle
EP1539980B1 (de) * 2002-08-01 2016-02-17 Nuevolution A/S MEHRSTUFENSYNTHESE VON MIT MATRIZEN ASSOZIIERTE MOLEKüLEN
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US8791053B2 (en) * 2002-09-27 2014-07-29 Mpm-Holding Aps Spatially encoded polymer matrix
EP2348124B1 (de) 2002-10-30 2013-12-11 Nuevolution A/S Synthese bifunktioneller Komplexe
US20040086895A1 (en) * 2002-11-06 2004-05-06 Crothers Donald M. Method of electrochemical detection of somatic cell mutations
US7090979B2 (en) * 2002-11-22 2006-08-15 The Regents Of The University Of California Derivatized versions of ligase enzymes for constructing DNA sequences
WO2004056994A2 (en) 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
WO2004092375A2 (en) * 2003-04-15 2004-10-28 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. Ligation-based synthesis of oligonucleotides with block structure
US20050069928A1 (en) * 2003-08-04 2005-03-31 Blue Heron Biotechnology, Inc. Methods for synthesis of defined polynucleotides
US11118215B2 (en) * 2003-09-18 2021-09-14 Nuevolution A/S Method for obtaining structural information concerning an encoded molecule and method for selecting compounds
JP2007508825A (ja) * 2003-10-15 2007-04-12 ブルー ヘロン バイオテクノロジー インコーポレイテッド ポリヌクレオチド生成のための固相方法
WO2005078122A2 (en) * 2004-02-17 2005-08-25 Nuevolution A/S Method for enrichment involving elimination by mismatch hybridisation
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
JP2008523786A (ja) * 2004-10-18 2008-07-10 コドン デバイシズ インコーポレイテッド 高忠実度合成ポリヌクレオチドのアセンブリ方法
WO2006065598A2 (en) * 2004-12-13 2006-06-22 Geneohm Sciences, Inc. Fluidic cartridges for electrochemical detection of dna
CN101133166B (zh) * 2005-03-10 2011-02-02 高晓莲 平行制备核酸及应用
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
DE102005029810B4 (de) * 2005-06-27 2008-11-13 Siemens Ag Verfahren zum Nachweis von Nukleotidsequenzen, Verwendung des Verfahrens und Testbesteck
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
PT3305900T (pt) 2005-12-01 2021-10-27 Nuevolution As Métodos de codificação enzimática para síntese eficaz de bibliotecas grandes
US8551697B1 (en) * 2005-12-09 2013-10-08 Applied Biosystems, Llc Electrochemical polynucleotide detection comprising ligation
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
JP2007275006A (ja) * 2006-04-10 2007-10-25 Hitachi High-Technologies Corp 核酸検出用プローブ作製法
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
AU2008343589A1 (en) 2007-12-19 2009-07-09 Centocor Ortho Biotech Inc. Design and generation of human de novo pIX phage display libraries via fusion to pIX or pVII, vectors, antibodies and methods
EP2285974A2 (de) 2008-05-14 2011-02-23 British Columbia Cancer Agency Branch Gensynthese durch konvergentes zusammenfügen von oligonukleotid-bauteilsätzen
KR20100089688A (ko) * 2009-02-04 2010-08-12 삼성전자주식회사 표적핵산의 서열을 분석하는 방법
ES2638324T3 (es) 2009-02-13 2017-10-19 X-Chem, Inc. Métodos de creación y examen de bibliotecas codificadas por ADN
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
EP3540059A1 (de) 2010-04-16 2019-09-18 Nuevolution A/S Bifunktionelle komplexe und verfahren zur herstellung sowie verwendung derartiger komplexe
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
IN2014CN02574A (de) 2011-09-07 2015-06-26 Chem Inc X
JP5920763B2 (ja) * 2011-10-05 2016-05-18 国立大学法人名古屋大学 蛍光標識オリゴヌクレオチド誘導体及びその利用
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
SG10201907347YA (en) 2012-07-13 2019-09-27 X Chem Inc Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20200140929A (ko) 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
US10273471B2 (en) 2013-03-15 2019-04-30 Gen 9, Inc. Compositions and methods for multiplex nucleic acids synthesis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
WO2015081114A2 (en) 2013-11-27 2015-06-04 Gen9, Inc. Libraries of nucleic acids and methods for making the same
AU2014363967A1 (en) * 2013-12-09 2017-01-05 Shawn Allen Long nucleic acid sequences containing variable regions
EP2883963A1 (de) * 2013-12-16 2015-06-17 Alacris Theranostics GmbH Verfahren zur Erzeugung von Oligonukleotid-Arrays mit In-situ-Blocksyntheseansatz
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
JP6769969B2 (ja) 2015-01-12 2020-10-14 １０エックス ジェノミクス， インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
US20180202072A1 (en) * 2015-07-20 2018-07-19 The Regents Of The University Of California Sequence-independent nucleic acid assembly
US10689690B2 (en) 2015-08-13 2020-06-23 Centrillion Technology Holdings Corporation Library construction using Y-adapters and vanishing restriction sites
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
JP6954899B2 (ja) 2015-12-04 2021-10-27 １０エックス ゲノミクス，インコーポレイテッド 核酸の解析のための方法及び組成物
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
US10907195B1 (en) * 2016-02-16 2021-02-02 Pharmaseq, Inc. Devices and methods for assembling extremely long DNA molecules
US10745814B2 (en) 2016-05-09 2020-08-18 President And Fellows Of Harvard College Enzymatic nucleic acid synthesis
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2018005720A1 (en) * 2016-06-30 2018-01-04 President And Fellows Of Harvard College Method of determining the molecular binding between libraries of molecules
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4029939B1 (de) 2017-01-30 2023-06-28 10X Genomics, Inc. Verfahren und systeme für eine einzelzellbarcodierung auf tröpfchenbasis
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (de) 2017-05-26 2023-07-05 10X Genomics, Inc. Einzelzellenanalyse von transposasezugänglichem chromatin
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3700672B1 (de) 2017-10-27 2022-12-28 10X Genomics, Inc. Verfahren zur probenvorbereitung und -analyse
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
EP3724658A1 (de) 2017-12-12 2020-10-21 10X Genomics, Inc. Systeme und verfahren zur einzelzellverarbeitung
CN111712579B (zh) 2017-12-22 2024-10-15 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
EP3737755B1 (de) 2018-01-12 2024-08-28 Camena Bioscience Limited Verfahren zur template-freien geometrischen enzymatischen nukleinsäuresynthese
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
SG11202101164TA (en) 2018-08-03 2021-03-30 10X Genomics Inc Methods and systems to minimize barcode exchange
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041360A1 (en) 2018-08-21 2020-02-27 Quidel Corporation Dbpa antibodies and uses thereof
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020150143A2 (en) 2019-01-14 2020-07-23 Camena Bioscience Limited Compositions and methods for template-free geometric enzymatic nucleic acid synthesis
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3924505B1 (de) 2019-02-12 2025-12-17 10X Genomics, Inc. Verfahren zur verarbeitung von nukleinsäuremolekülen
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
EP3947722A1 (de) 2019-03-27 2022-02-09 10X Genomics, Inc. Systeme und verfahren zur verarbeitung von rna aus zellen
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
EP4077376A2 (de) 2019-12-19 2022-10-26 Quidel Corporation Monoklonale antikörperfusionen
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
CN117015617B (zh) 2021-02-23 2025-04-04 10X基因组学有限公司 基于探针的核酸和蛋白质分析
CN121969766A (zh) * 2023-08-31 2026-05-01 因美纳有限公司 适体检测技术

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5093251A (en) * 1986-05-23 1992-03-03 California Institute Of Technology Cassette method of gene synthesis
GB8808892D0 (en) * 1988-04-15 1988-05-18 British Bio Technology Gene synthesis
WO1990000626A1 (en) * 1988-07-14 1990-01-25 Baylor College Of Medicine Solid phase assembly and reconstruction of biopolymers
JP3080178B2 (ja) * 1991-02-18 2000-08-21 東洋紡績株式会社 核酸配列の増幅方法およびそのための試薬キット
JP3085409B2 (ja) * 1991-03-29 2000-09-11 東洋紡績株式会社 標的核酸配列の検出方法およびそのための試薬キット
WO1993017127A1 (en) * 1992-02-20 1993-09-02 The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Boomerand dna amplification
AU672969B2 (en) * 1992-03-10 1996-10-24 United States of America, as represented by The Secretary, Department of Health & Human Services, The Exchangeable template reaction
DE4343591A1 (de) * 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
CA2258570A1 (en) * 1996-06-17 1997-12-24 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs

Also Published As

Publication number Publication date
AU770217B2 (en) 2004-02-19
EP1129215B1 (de) 2004-04-28
EP1129215A1 (de) 2001-09-05
AU5783899A (en) 2000-06-05
DE69916877D1 (de) 2004-06-03
JP2002531071A (ja) 2002-09-24
US5942609A (en) 1999-08-24
CA2353076A1 (en) 2000-05-25
DE69916877T2 (de) 2005-03-24
WO2000029616A1 (en) 2000-05-25
JP3544945B2 (ja) 2004-07-21

Similar Documents

Publication Publication Date Title
ATE265545T1 (de) Ligationsassemblierung und detektion von polynucleotiden auf einem festträger
DK1427852T3 (da) Enkelnukleotidamplikation og -detektion med polymerase
WO2002083839A3 (en) P450 single nucleotide polymorphism biochip analysis
AU2002351193A8 (en) Parallel polymorphism scoring by amplification and error correction
RU95106503A (ru) Способ увеличения концентрации кислоты-мишени, способы определения присутствия выбранного нуклеотида, наборы
DE60035691D1 (de) Verfahren zur Erkennung von Einzel-Nukleotid-Polymorphismen
NZ506333A (en) An assay for detecting and quantifying the presence of particular nucleic acid (DNA or RNA) sequences
EA200300991A1 (ru) Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы
WO2005047543A3 (en) Ligation assay
DE60143960D1 (de) Äuren
ATE322554T1 (de) Dns charakterisierung
ATE329061T1 (de) Verfahren zur reduzierung unspezifischer amplifikation bei pcr
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
WO2005098042A3 (en) Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
DE69622347D1 (de) Oligonukleotid-Primer für Amplifizierung von HCV-Nukleinsäure
Park et al. Target DNA induced switches of DNA polymerase activity
DE60143314D1 (de) Estimmung der präsenz von cryptosporidium organismen in einer testprobe
WO2002095057A3 (en) Pairs of nucleic acid probes with interactive signaling moieties and nucleic acid probes with enhanced hybridization efficiency and specificity
WO2004050911A3 (en) Method for lowering both sequence variations and increase of base lines effects in diagnostic hybridisation assay, assay for performing such a method and probe for use in the assay
DK1815013T3 (da) Sammensætning til at amplificere nucleinsyrer
ATE455866T1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
ATE207544T1 (de) Amplikationsprimern und nukleinsäuresonden zum nachweis von coccidioides immites
ATE335849T1 (de) Verfahren und nukleinsäuren zum nachweis von brauereirelevanten mikroorganismen
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
ATE455864T1 (de) Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties