ATE324465T1 - Nachweismethode mittels verstärkter silberfärbung - Google Patents

Nachweismethode mittels verstärkter silberfärbung

Info

Publication number
ATE324465T1
ATE324465T1 AT01954641T AT01954641T ATE324465T1 AT E324465 T1 ATE324465 T1 AT E324465T1 AT 01954641 T AT01954641 T AT 01954641T AT 01954641 T AT01954641 T AT 01954641T AT E324465 T1 ATE324465 T1 AT E324465T1
Authority
AT
Austria
Prior art keywords
tinting
detection method
silver
increased silver
increased
Prior art date
Application number
AT01954641T
Other languages
English (en)
Inventor
Robert L Letsinger
Viswanadham Garimella
Original Assignee
Univ Northwestern
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Northwestern filed Critical Univ Northwestern
Application granted granted Critical
Publication of ATE324465T1 publication Critical patent/ATE324465T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/137—Metal/ion, e.g. metal label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Coloring (AREA)
AT01954641T 2000-07-11 2001-07-11 Nachweismethode mittels verstärkter silberfärbung ATE324465T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21778200P 2000-07-11 2000-07-11

Publications (1)

Publication Number Publication Date
ATE324465T1 true ATE324465T1 (de) 2006-05-15

Family

ID=22812486

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01954641T ATE324465T1 (de) 2000-07-11 2001-07-11 Nachweismethode mittels verstärkter silberfärbung

Country Status (8)

Country Link
US (2) US6602669B2 (de)
EP (1) EP1360318B1 (de)
JP (1) JP3605607B2 (de)
AT (1) ATE324465T1 (de)
AU (2) AU7687001A (de)
CA (1) CA2415494C (de)
DE (1) DE60119170T2 (de)
WO (1) WO2002004681A2 (de)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7169556B2 (en) * 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
DE69737598T2 (de) 1996-07-29 2007-12-27 Nanosphere Inc., Skokie Nanopartikel mit daran angehefteten oligonukleotiden sowie deren verwendungen
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US20050037397A1 (en) * 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
IL126776A (en) * 1998-10-27 2001-04-30 Technion Res & Dev Foundation A method of investing gold
EP1135682B1 (de) 1998-11-30 2007-07-11 Nanosphere, Inc. Nanopartikel mit polymerschalen
US7364920B2 (en) * 1999-10-27 2008-04-29 Technion Research And Development Foundation Ltd. Method for gold deposition
CA2396113C (en) * 2000-01-13 2009-04-07 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
DE60119170T2 (de) 2000-07-11 2007-05-24 Northwestern University, Evanston Nachweismethode mittels verstärkter silberfärbung
JP2004524528A (ja) * 2001-02-14 2004-08-12 ユニバーシティ オブ メリーランド，ボルチモア 放射減衰操作
US20060040286A1 (en) * 2001-03-28 2006-02-23 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20030113740A1 (en) * 2001-04-26 2003-06-19 Mirkin Chad A. Oligonucleotide-modified ROMP polymers and co-polymers
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
WO2002096262A2 (en) 2001-05-25 2002-12-05 Northwestern University Non-alloying core shell nanoparticles
US7186814B2 (en) 2001-11-09 2007-03-06 Nanosphere, Inc. Bioconjugate-nanoparticle probes
GR1004178B (el) * 2001-11-29 2003-03-05 "����������" Ολοκληρωμενος οπτοηλεκτρονικος βιοαισθητηρας πυριτιου για ανιχνευση βιομοριων επισημασμενων με χρωμοφορες ομαδες ή νανοσωματιδια
US7361310B1 (en) 2001-11-30 2008-04-22 Northwestern University Direct write nanolithographic deposition of nucleic acids from nanoscopic tips
EP1461596B1 (de) * 2001-11-30 2013-07-17 Northwestern University Direktes-schreiben-nanolithographische abscheidung von nukleinsäuren aus nanoskopischen spitzen
US20030211488A1 (en) 2002-05-07 2003-11-13 Northwestern University Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
EP1540006B1 (de) * 2002-07-02 2009-01-07 Nanosphere, Inc. Nanopartikel-polyanion-konjugate sowie verfahren zur verwendung davon beim nachweis von analyten
EP1387169B1 (de) * 2002-08-02 2006-05-24 Sony Deutschland GmbH Verfahren zur Bindung hydophiler Substanzen an hydrophile Makromoleküle und Immobilisierung derselben auf hydrophoben Oberflächen
US20040101859A1 (en) * 2002-11-25 2004-05-27 Cepheid Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
EP1439392A3 (de) * 2003-01-15 2005-07-27 Agilent Technologies, Inc. Biosensorsysteme und Verfahren zur Detektion der Anwesenheit von Biomolekülen
US20040161862A1 (en) * 2003-02-15 2004-08-19 Golovlev Valeri V. Method of visualization and quantification of biopolymer molecules immobilized on solid support
US20080050842A1 (en) * 2003-02-15 2008-02-28 Golovlev Valeri V Method of visualization and quanitification of biopolymer molecules immobilized on solid support
JP2007524347A (ja) * 2003-02-27 2007-08-30 ナノスフェアー　インコーポレイテッド マイクロアレイ形式のアッセイにおける、万能ナノ粒子プローブを用いた標識不要の遺伝子発現プロファイリング
JP2007516426A (ja) * 2003-05-16 2007-06-21 ユニバーシティー オブ ロチェスター オリゴヌクレオチドの検出のための比色法および蛍光法
US20060166249A1 (en) * 2003-05-16 2006-07-27 University Of Rochester Methods for separating short single-stranded nucleic acid from long single-and double-stranded nucleic acid, and associated biomolecular assays
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US20090163375A1 (en) 2003-09-09 2009-06-25 Bowman Christopher N Use of Photopolymerization for Amplification and Detection of a Molecular Recognition Event
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
WO2005066613A1 (en) * 2003-12-31 2005-07-21 President And Fellows Of Harvard College Assay device and method
SE0401033D0 (sv) * 2004-04-20 2004-04-20 Amersham Biosciences Ab Device and method for protein analysis
JP2008507296A (ja) * 2004-07-26 2008-03-13 ナノスフェアー　インコーポレイテッド 混合培養物中でメチシリン耐性黄色ブドウ球菌とメチシリン感受性黄色ブドウ球菌とを区別する方法
US20060068398A1 (en) * 2004-09-24 2006-03-30 Cepheid Universal and target specific reagent beads for nucleic acid amplification
EP2351857B1 (de) * 2005-02-17 2014-10-29 TrovaGene, Inc. Zusammensetzungen und Verfahren zum Nachweis von für Krankheitserreger spezifischen Nukleinsäuren in Urin
EP1880206B1 (de) * 2005-05-09 2012-11-14 Affymetrix, Inc. Multiplex-einfang von nukleinsäuren
WO2006125124A2 (en) * 2005-05-18 2006-11-23 Nanosphere, Inc. Substrate functionalization method for high sensitivity applications
WO2006138145A1 (en) 2005-06-14 2006-12-28 Northwestern University Nucleic acid functionalized nanoparticles for therapeutic applications
WO2007002567A2 (en) * 2005-06-23 2007-01-04 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
JP2009505106A (ja) * 2005-08-19 2009-02-05 ナノスフェアー インコーポレイテッド Ｄｎａ及び抗体を含むハイブリッド基板を調製するための方法及びその使用
US20090280188A1 (en) * 2006-06-23 2009-11-12 Northwestern University Asymmetric functionalizated nanoparticles and methods of use
EP2121987B1 (de) 2007-02-09 2012-06-13 Northwestern University Partikel zur erkennung intrazellulärer ziele
CA2679586A1 (en) * 2007-02-27 2008-10-23 Northwestern University Molecule attachment to nanoparticles
JP5276655B2 (ja) * 2007-06-01 2013-08-28 アトノミックス アクティーゼルスカブ 標的検体の検出用のナノ粒子を用いた生体表面弾性波（ｓａｗ）共振器増幅
KR100898623B1 (ko) * 2007-06-27 2009-05-21 한국과학기술원 금 나노입자를 이용하여 바이오물질을 기판에 고정화시키는방법
WO2009035357A2 (en) * 2007-09-16 2009-03-19 Instytut Hodowli I Aklimatyzacji Roslin Immunological tests for the presence of bacteria which make use of antibodies obtained using a specific method
JP5091075B2 (ja) * 2007-09-28 2012-12-05 富士フイルム株式会社 イムノクロマトグラフ方法
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
JP4980946B2 (ja) * 2008-02-12 2012-07-18 富士フイルム株式会社 ブロッティング検出方法
US8962260B2 (en) 2008-05-20 2015-02-24 Rapid Pathogen Screening, Inc. Method and device for combined detection of viral and bacterial infections
US8815609B2 (en) 2008-05-20 2014-08-26 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with diverting zone
US8609433B2 (en) 2009-12-04 2013-12-17 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with sample compressor
US20130196310A1 (en) 2008-05-20 2013-08-01 Rapid Pathogen Screening, Inc. Method and Device for Combined Detection of Viral and Bacterial Infections
US9068981B2 (en) 2009-12-04 2015-06-30 Rapid Pathogen Screening, Inc. Lateral flow assays with time delayed components
EP2365803B1 (de) 2008-11-24 2017-11-01 Northwestern University Polyvalente rna-nanoteilchen-zusammensetzungen
US20100233270A1 (en) 2009-01-08 2010-09-16 Northwestern University Delivery of Oligonucleotide-Functionalized Nanoparticles
CA2779099C (en) 2009-10-30 2021-08-10 Northwestern University Templated nanoconjugates
CA2805720A1 (en) 2010-06-17 2011-12-22 Abaxis, Inc. Rotors for immunoassays
CN102226811B (zh) * 2011-04-02 2013-10-23 长春市大格药物研究所 一种检测尿液中心脏型脂肪酸结合蛋白诊断试纸的制备方法
US9605304B2 (en) * 2011-07-20 2017-03-28 The Hong Kong Polytechnic University Ultra-stable oligonucleotide-gold and-silver nanoparticle conjugates and method of their preparation
AU2012308302A1 (en) 2011-09-14 2014-03-20 Northwestern University Nanoconjugates able to cross the blood-brain barrier
US9051583B2 (en) 2011-12-19 2015-06-09 Northwestern University Modified silica shell particles, and methods of making and using the same
CN102759526B (zh) * 2012-06-28 2014-08-27 宁波大学 一种金标银染定量检测汞离子的方法及其试剂盒
EP3024936B1 (de) 2013-07-25 2019-09-04 Exicure, Inc. Sphärische nukleinsäurebasierte konstrukte als immunstimulatoren für prophylaktische und therapeutische verwendung
US11213593B2 (en) 2014-11-21 2022-01-04 Northwestern University Sequence-specific cellular uptake of spherical nucleic acid nanoparticle conjugates
US10808287B2 (en) 2015-10-23 2020-10-20 Rapid Pathogen Screening, Inc. Methods and devices for accurate diagnosis of infections
EP3387154A4 (de) * 2015-12-09 2019-05-15 Intuitive Biosciences, Inc. Automatisiertes silberveredelungssystem
US11300386B2 (en) 2015-12-31 2022-04-12 Dupont Safety & Construction, Inc. Ballistic materials incorporating spunlaced nonwovens
WO2018039629A2 (en) 2016-08-25 2018-03-01 Northwestern University Micellar spherical nucleic acids from thermoresponsive, traceless templates
WO2018209178A1 (en) 2017-05-11 2018-11-15 The Research Foundation For The State University Of New York Method and kit for determining the presence of monosodium urate crystals in joint synovial fluid
WO2018209270A1 (en) 2017-05-11 2018-11-15 Northwestern University Adoptive cell therapy using spherical nucleic acids (snas)
EP3820610A1 (de) 2018-07-12 2021-05-19 Luminex Corporation Systeme und verfahren zur durchführung variabler probenvorbereitung und analyseverfahren
CN116465883A (zh) * 2023-04-27 2023-07-21 宁波大学 一种检测银离子的方法

Family Cites Families (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4193983A (en) 1978-05-16 1980-03-18 Syva Company Labeled liposome particle compositions and immunoassays therewith
NL7807532A (nl) 1978-07-13 1980-01-15 Akzo Nv Metaal-immunotest.
US4318707A (en) 1978-11-24 1982-03-09 Syva Company Macromolecular fluorescent quencher particle in specific receptor assays
US4256834A (en) 1979-04-09 1981-03-17 Syva Company Fluorescent scavenger particle immunoassay
US4261968A (en) 1979-05-10 1981-04-14 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4650770A (en) 1981-04-27 1987-03-17 Syntex (U.S.A.) Inc. Energy absorbing particle quenching in light emitting competitive protein binding assays
US4713348A (en) 1983-04-05 1987-12-15 Syntex (U.S.A.) Inc. Fluorescent multiparameter particle analysis
US5288609A (en) 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US4868104A (en) 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5137827A (en) 1986-03-25 1992-08-11 Midwest Research Technologies, Inc. Diagnostic element for electrical detection of a binding reaction
US5514602A (en) 1986-06-09 1996-05-07 Ortho Diagnostic Systems, Inc. Method of producing a metal sol reagent containing colloidal metal particles
US5360895A (en) 1987-04-22 1994-11-01 Associated Universities, Inc. Derivatized gold clusters and antibody-gold cluster conjugates
US4853335A (en) 1987-09-28 1989-08-01 Olsen Duane A Colloidal gold particle concentration immunoassay
GB8800702D0 (en) 1988-01-13 1988-02-10 Nycomed As Test method & reagent kit therefor
WO1990002205A1 (en) 1988-08-25 1990-03-08 Angenics, Inc. Detection of nucleic acid sequences using particle agglutination
US5460831A (en) 1990-06-22 1995-10-24 The Regents Of The University Of California Targeted transfection nanoparticles
GB9019512D0 (en) 1990-09-06 1990-10-24 Ici Plc Assay method
JPH06510363A (ja) * 1990-10-29 1994-11-17 ディカルブ　プラント　ジェネティクス 磁気性粒子を使用する生物学的材料の単離
US5665582A (en) 1990-10-29 1997-09-09 Dekalb Genetics Corp. Isolation of biological materials
US5294369A (en) 1990-12-05 1994-03-15 Akzo N.V. Ligand gold bonding
WO1993010564A1 (en) 1991-11-22 1993-05-27 The Regents Of The University Of California Semiconductor nanocrystals covalently bound to solid inorganic surfaces using self-assembled monolayers
US5225064A (en) 1992-01-15 1993-07-06 Enzyme Technology Research Group, Inc. Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
GB9212164D0 (en) 1992-06-09 1992-07-22 Medical Res Council Preparation of nucleic acids
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
US5384265A (en) 1993-03-26 1995-01-24 Geo-Centers, Inc. Biomolecules bound to catalytic inorganic particles, immunoassays using the same
US5637508A (en) 1993-03-26 1997-06-10 Geo-Centers, Inc. Biomolecules bound to polymer or copolymer coated catalytic inorganic particles, immunoassays using the same and kits containing the same
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
EP0630974A3 (de) 1993-06-25 1995-11-15 Clinical Diagnostic Syst Verfahren und Testkit zum Nachweis von anorganischen Orthophosphat bei der Amplifizierung von Zielnukleinsäuren.
US5543158A (en) 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
JPH07227299A (ja) 1994-02-14 1995-08-29 Kyoto Daiichi Kagaku:Kk Ｄｎａの特定塩基配列の検出方法及びその装置
US5521289A (en) 1994-07-29 1996-05-28 Nanoprobes, Inc. Small organometallic probes
US5599668A (en) 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
US5609907A (en) 1995-02-09 1997-03-11 The Penn State Research Foundation Self-assembled metal colloid monolayers
US6025202A (en) 1995-02-09 2000-02-15 The Penn State Research Foundation Self-assembled metal colloid monolayers and detection methods therewith
US6586193B2 (en) 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
JP2000510582A (ja) 1996-04-25 2000-08-15 ゼニコン・サイエンシーズ・コーポレーション 微粒子標識を使用した分析物アッセイ
DE69737598T2 (de) 1996-07-29 2007-12-27 Nanosphere Inc., Skokie Nanopartikel mit daran angehefteten oligonukleotiden sowie deren verwendungen
US7169556B2 (en) * 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) * 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6750016B2 (en) * 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6984491B2 (en) * 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6242264B1 (en) 1996-09-04 2001-06-05 The Penn State Research Foundation Self-assembled metal colloid monolayers having size and density gradients
WO1998017317A2 (de) 1996-10-23 1998-04-30 Skw Trostberg Aktiengesellschaft Verfahren zur herstellung von biologisch aktiven polymernanopartikel-nucleinsäure-konjugaten
US5830986A (en) 1996-10-28 1998-11-03 Massachusetts Institute Of Technology Methods for the synthesis of functionalizable poly(ethylene oxide) star macromolecules
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US5922537A (en) * 1996-11-08 1999-07-13 N.o slashed.AB Immunoassay, Inc. Nanoparticles biosensor
US5939021A (en) 1997-01-23 1999-08-17 Hansen; W. Peter Homogeneous binding assay
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
AUPP004497A0 (en) 1997-10-28 1997-11-20 University Of Melbourne, The Stabilized particles
US6149868A (en) 1997-10-28 2000-11-21 The Penn State Research Foundation Surface enhanced raman scattering from metal nanoparticle-analyte-noble metal substrate sandwiches
JP2002509443A (ja) 1997-10-31 2002-03-26 ジェン−プローブ・インコーポレーテッド 核酸検出法
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US5972615A (en) * 1998-01-21 1999-10-26 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6306610B1 (en) 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6203989B1 (en) 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
EP1135682B1 (de) 1998-11-30 2007-07-11 Nanosphere, Inc. Nanopartikel mit polymerschalen
US6277489B1 (en) 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
WO2001000876A1 (en) 1999-06-25 2001-01-04 Mirkin Chad A Nanoparticles having oligonucleotides attached thereto and uses therefor
CA2396113C (en) * 2000-01-13 2009-04-07 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
CA2402955C (en) 2000-03-28 2010-03-09 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
AU5667901A (en) 2000-05-11 2001-11-20 Center For Advanced Science And Technology Incubation, Ltd. Polymer composition for forming surface of biosensor
DE60119170T2 (de) * 2000-07-11 2007-05-24 Northwestern University, Evanston Nachweismethode mittels verstärkter silberfärbung
CA2418502A1 (en) 2000-08-11 2002-03-07 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
CN1507357A (zh) 2000-10-31 2004-06-23 PRҩƷ���޹�˾ 提高生物活性分子传递的方法和组合物
WO2002046483A2 (en) 2000-12-06 2002-06-13 Northwestern University Silver stain removal from dna detection chips by cyanide etching or sonication
WO2002046472A2 (en) 2000-12-08 2002-06-13 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20030113740A1 (en) * 2001-04-26 2003-06-19 Mirkin Chad A. Oligonucleotide-modified ROMP polymers and co-polymers
WO2002096262A2 (en) * 2001-05-25 2002-12-05 Northwestern University Non-alloying core shell nanoparticles
US7147687B2 (en) * 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
ATE423975T1 (de) * 2001-08-03 2009-03-15 Nanosphere Inc Nanopartikelabbildungssystem und -verfahren
US7186814B2 (en) * 2001-11-09 2007-03-06 Nanosphere, Inc. Bioconjugate-nanoparticle probes
US20030211488A1 (en) * 2002-05-07 2003-11-13 Northwestern University Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
EP1540006B1 (de) * 2002-07-02 2009-01-07 Nanosphere, Inc. Nanopartikel-polyanion-konjugate sowie verfahren zur verwendung davon beim nachweis von analyten

Also Published As

Publication number Publication date
US20040101889A1 (en) 2004-05-27
JP3605607B2 (ja) 2004-12-22
US6602669B2 (en) 2003-08-05
CA2415494C (en) 2007-09-18
DE60119170T2 (de) 2007-05-24
US20020034756A1 (en) 2002-03-21
CA2415494A1 (en) 2002-01-17
WO2002004681A3 (en) 2003-08-07
DE60119170D1 (de) 2006-06-01
JP2004502950A (ja) 2004-01-29
WO2002004681A2 (en) 2002-01-17
EP1360318B1 (de) 2006-04-26
AU2001276870B2 (en) 2006-07-20
AU7687001A (en) 2002-01-21
EP1360318A2 (de) 2003-11-12

Similar Documents

Publication Publication Date Title
ATE324465T1 (de) Nachweismethode mittels verstärkter silberfärbung
DE60135912D1 (de) Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
DE60322880D1 (de) Einfache katalytische dna-biosensoren für ionen auf grundlage von farbveränderungen
ATE515488T1 (de) Click-chemie zur herstellung von reportermolekülen
DE60118527D1 (de) Sensible und multiplexe diagnostische tests zur proteinanalyse
WO2006128138A3 (en) Biodetection by nucleic acid-templated chemistry
EP0722508A4 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
DE60029092D1 (de) Verfahren zur detektion von nukleinsäuren, welche auf krebs hinweisen
DE602004021185D1 (de) Auf bio-barcodes beruhender nachweis von zielanalyten
DE69942451D1 (de) Methode zum schnellen identifizieren von kleinen organischen liganden
DE69939896D1 (de) Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits
WO2002090572A3 (en) Nucleic acid detection in pooled samples
DE60231606D1 (de) Zirkuläre sonden amplifizierung (cpa) von zirkularisierten nucleinsäure-molekülen
ATE454469T1 (de) Für den nachweis von nukleinsäuren kompetente biologische rohderivate
WO2007012329A3 (de) Rna-abhängige rna-polymerase, verfahren und kits zur amplifikation und / oder markierung von rna
EP2004852A4 (de) Amplifikation von dna-fragmenten
DE602004031022D1 (de) Nukleinsäuresequenzidentifikation
DE60129809D1 (de) In vitro evolution von nukleinsäuren und kodierten polypeptiden
BR0113257A (pt) Proteìna eer-7 da famìlia do gene da lisil oxidase, método para produzi-la, para detectá-la e detectar sua expressão, fragmento de polipeptìdeo da mesma, ácido nucléico isolado e vetor compreendendo o mesmo, e sistema de ensaio
EP1083980A4 (de) Modulation molekularer wechselwirkungspositionen in rns und anderen biomolekülen
ATE335849T1 (de) Verfahren und nukleinsäuren zum nachweis von brauereirelevanten mikroorganismen
WO2000029624A3 (en) Methods and compositions for detection of nucleic acid sequences and signal amplification
ATE386140T1 (de) Methoden und reagenzien zur in situ amplifizierung
EP1213351A3 (de) Nukleinsäure-Enzym, das durch Erkennung eines anderen Moleküls, die Aktivität zur Spaltung einer Ziel-RNA gewinnt

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties