ATE274587T1 - 5'-nukleasen aus thermostabilen dna-polymerasen - Google Patents

5'-nukleasen aus thermostabilen dna-polymerasen

Info

Publication number
ATE274587T1
ATE274587T1 AT94919345T AT94919345T ATE274587T1 AT E274587 T1 ATE274587 T1 AT E274587T1 AT 94919345 T AT94919345 T AT 94919345T AT 94919345 T AT94919345 T AT 94919345T AT E274587 T1 ATE274587 T1 AT E274587T1
Authority
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Austria
Prior art keywords
nucleases
nucleic acid
dna polymerases
thermostable dna
cleaving
Prior art date
Application number
AT94919345T
Other languages
English (en)
Inventor
James E Dahlberg
Victor I Lyamichev
Mary Ann D Brow
Original Assignee
Third Wave Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Third Wave Tech Inc filed Critical Third Wave Tech Inc
Application granted granted Critical
Publication of ATE274587T1 publication Critical patent/ATE274587T1/de

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
AT94919345T 1993-06-04 1994-06-06 5'-nukleasen aus thermostabilen dna-polymerasen ATE274587T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/073,384 US5541311A (en) 1992-12-07 1993-06-04 Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
PCT/US1994/006253 WO1994029482A1 (en) 1993-06-04 1994-06-06 5' nucleases derived from thermostable dna polymerase

Publications (1)

Publication Number Publication Date
ATE274587T1 true ATE274587T1 (de) 2004-09-15

Family

ID=22113384

Family Applications (2)

Application Number Title Priority Date Filing Date
AT94919345T ATE274587T1 (de) 1993-06-04 1994-06-06 5'-nukleasen aus thermostabilen dna-polymerasen
AT04020089T ATE373726T1 (de) 1993-06-04 1994-06-06 Methode zur detektion eines dna zielmoleküls

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT04020089T ATE373726T1 (de) 1993-06-04 1994-06-06 Methode zur detektion eines dna zielmoleküls

Country Status (10)

Country Link
US (3) US5541311A (de)
EP (2) EP0711361B1 (de)
JP (4) JPH08511684A (de)
AT (2) ATE274587T1 (de)
AU (1) AU694736B2 (de)
CA (2) CA2320666C (de)
DE (2) DE69433966D1 (de)
DK (1) DK1505160T3 (de)
ES (1) ES2293141T3 (de)
WO (1) WO1994029482A1 (de)

Families Citing this family (174)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US7150982B2 (en) * 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
US5994069A (en) * 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6872816B1 (en) * 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7045289B2 (en) * 1991-09-09 2006-05-16 Third Wave Technologies, Inc. Detection of RNA Sequences
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5843654A (en) * 1992-12-07 1998-12-01 Third Wave Technologies, Inc. Rapid detection of mutations in the p53 gene
US6673616B1 (en) * 1992-12-07 2004-01-06 Third Wave Technologies, Inc. Methods and compositions for characterizing nucleic acids
US20060035257A1 (en) * 1992-12-07 2006-02-16 Dahlberg James E Cleavage of nucleic acids
US6372424B1 (en) * 1995-08-30 2002-04-16 Third Wave Technologies, Inc Rapid detection and identification of pathogens
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US5547859A (en) * 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
WO2000033244A2 (en) * 1998-11-27 2000-06-08 Synaptics (Uk) Limited Position sensor
WO1996014405A2 (en) * 1994-11-04 1996-05-17 Molecular Biology Resources, Inc. Biologically active fragments of thermus flavus dna polymerase
CA2203627C (en) * 1994-11-09 2000-06-06 James E. Dahlberg Rapid detection and identification of nucleic acid variants and pathogens
ES2185719T3 (es) * 1994-12-23 2003-05-01 Dade Behring Inc Deteccion de acidos nucleicos por formacion de un producto catalizada por diana.
DE19525632C2 (de) * 1995-07-14 1997-07-17 Bag Biolog Analysensystem Gmbh Sequenzspezifisches Nukleinsäurenachweisverfahren und Reagenz-System zu dessen Durchführung
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US20080160524A1 (en) * 1996-01-24 2008-07-03 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
US7122364B1 (en) * 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US7195871B2 (en) * 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
ATE414793T1 (de) * 1996-01-24 2008-12-15 Third Wave Tech Inc Invasive spaltung von nukleinsäuren
US7527928B2 (en) * 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US6913881B1 (en) 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US20080220425A1 (en) * 1996-01-24 2008-09-11 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US7432048B2 (en) * 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US6090606A (en) * 1996-01-24 2000-07-18 Third Wave Technologies, Inc. Cleavage agents
US6140053A (en) * 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
CA2273204C (en) * 1996-11-29 2008-10-14 Third Wave Technologies, Inc. Fen-1 endonucleases, mixtures and cleavage methods
DE69841023D1 (de) 1997-03-12 2009-09-10 Applied Biosystems Llc DNA Polymerasen mit verbesserten Fähigkeiten zum Einbau von markierten Nukleotiden
US6251638B1 (en) 1997-05-30 2001-06-26 Diagen Corporation Methods for detection of nucleic acid sequences in urine
US7049101B1 (en) * 1997-08-06 2006-05-23 Diversa Corporation Enzymes having high temperature polymerase activity and methods of use thereof
US7056703B2 (en) * 1997-08-06 2006-06-06 Diversa Corporation Polypeptides having polymerase activity and methods of use thereof
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6159693A (en) * 1998-03-13 2000-12-12 Promega Corporation Nucleic acid detection
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6235480B1 (en) * 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
EP1090293B2 (de) * 1998-06-24 2019-01-23 Illumina, Inc. Dekodierung von matrixartig-angeordneten Sensoren durch Mikropartikel
US6593084B2 (en) 1998-10-13 2003-07-15 Robert E. Bird Carcinogen assay
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
WO2000063437A2 (en) 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
EP1175501A4 (de) * 1999-05-12 2002-11-27 Invitrogen Corp Zusammensetzungen und verfahren zur erhöhung der empfindlichkeit und spezifität der nukleinsäuresynthese
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8080380B2 (en) * 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US7824859B2 (en) * 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7838225B2 (en) * 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US6893819B1 (en) * 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
CA2389268A1 (en) * 1999-11-24 2001-06-14 Incyte Genomics, Inc. Normalization controls and duplex probes for quantitative hybridization reactions
US6423535B1 (en) 1999-11-24 2002-07-23 Incyte Genomics, Inc. Normalization control for hybridization reactions
US7361488B2 (en) * 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
WO2001057268A2 (en) 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US20050214825A1 (en) * 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
DK1259643T3 (da) * 2000-02-07 2009-02-23 Illumina Inc Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming
ATE412774T1 (de) 2000-02-16 2008-11-15 Illumina Inc Parallele genotypisierung mehrerer patientenproben
DE60131903T2 (de) * 2000-10-24 2008-11-27 The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto Direkte multiplex charakterisierung von genomischer dna
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20030092157A1 (en) * 2001-03-16 2003-05-15 Hayden Michael R. Compositions, screening systems and methods for modulating HDL cholesterol and triglyceride levels
WO2002097123A2 (en) * 2001-05-25 2002-12-05 Xenon Genetics, Inc. Diagnostic methods for cardiovascular disease, low hdl-cholesterol levels, and high triglyceride levels
WO2003004632A2 (en) * 2001-07-06 2003-01-16 Amersham Biosciences Corp Novel dna polymerases having amino acid substitutions and homologs thereof
AU2002327236A1 (en) 2001-07-12 2003-01-29 Illumina, Inc. Multiplex nucleic acid reactions
CA2459718A1 (en) * 2001-09-14 2003-08-14 Applera Corporation Thermus scotoductus nucleic acid polymerases
US20070020622A1 (en) * 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
JP2005525787A (ja) * 2001-10-24 2005-09-02 シンギュレックス・インコーポレイテッド プローブとの相互作用による遺伝子ハプロタイプの検出方法
US7052877B2 (en) 2001-11-30 2006-05-30 Applera Corporation Thermus brockianus nucleic acid polymerases
AU2002350247A1 (en) * 2001-11-30 2003-06-17 Applera Corporation Thermus igniterrae nucleic acid polymerases
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
WO2003064679A2 (en) * 2002-01-30 2003-08-07 Id Biomedical Corporation Methods for detecting vancomycin-resistant microorganisms and compositions therefor
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20050003355A1 (en) * 2002-04-22 2005-01-06 Manchun Lu Single nucleotide polymorphism analysis using surface invasive cleavage reactions
US20060147938A1 (en) * 2002-11-14 2006-07-06 Third Wave Technologies, Inc. CFTR allele detection assays
US7312033B2 (en) * 2002-11-14 2007-12-25 Third Wave Technologies, Inc. CFTR allele detection assays
US20040096871A1 (en) * 2002-11-14 2004-05-20 Molly Accola CFTR allele detection assays
AU2003298672A1 (en) * 2002-11-19 2005-01-28 Singulex, Inc. Detection of target molecules through interaction with probes
US8206904B2 (en) * 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
JP4230457B2 (ja) 2002-12-18 2009-02-25 サード・ウェーブ・テクノロジーズ・インク 低分子核酸の検出方法
US7700750B2 (en) * 2003-01-09 2010-04-20 Third Wave Technologies, Inc. Connexin allele detection assays
WO2004065000A1 (en) 2003-01-21 2004-08-05 Illumina Inc. Chemical reaction monitor
US20040161749A1 (en) * 2003-01-24 2004-08-19 Hall Jeff G. 3' end tagged oligonucleotides
US6943768B2 (en) 2003-02-21 2005-09-13 Xtellus Inc. Thermal control system for liquid crystal cell
AU2004225520A1 (en) * 2003-03-25 2004-10-14 Stratagene DNA polymerase fusions and uses thereof
US20040259100A1 (en) 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005019419A2 (en) * 2003-07-31 2005-03-03 Singulex, Inc. Co-detection of single polypeptide and polynucleotide molecules
US20050136414A1 (en) * 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
CA2552949C (en) 2004-01-07 2012-10-02 Third Wave Technologies, Inc. Determination of hepatitis c virus genotype
US7417133B2 (en) 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
EP1574583A1 (de) * 2004-03-10 2005-09-14 Roche Diagnostics GmbH Verfahren zur Isolierung von Bakterien aus biologischen Proben
WO2007008276A2 (en) * 2005-05-03 2007-01-18 Ensemble Discovery Corporation Turnover probes and use thereof for nucleic acid detection
CA2608151A1 (en) * 2005-05-11 2006-11-16 Stratagene California Snapback oligonucleotide probe
US20080014124A1 (en) * 2005-08-04 2008-01-17 Third Wave Technologies, Inc. Sample processing devices, systems and methods
US7482127B2 (en) * 2005-11-03 2009-01-27 Third Wave Technologies, Inc. Nucleic acid detection assays employing blocker oligonucleotides
US20070207455A1 (en) * 2005-11-17 2007-09-06 Third Wave Technologies, Inc. Compositions and methods for detecting an HCV-1 subtype
US7981606B2 (en) * 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
EP2021506B1 (de) * 2006-04-28 2016-03-16 Igor Kutyavin Verwendung von pcr-amplifikationsprodukten mit elementen sekundärer struktur für verbesserten pcr-basierten nukleinsäurenachweis
WO2007127992A2 (en) 2006-04-28 2007-11-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates
US20080125333A1 (en) * 2006-05-31 2008-05-29 Antara Biosciences Inc. Devices and kits for detecting one or more target agents
KR101105564B1 (ko) 2006-06-01 2012-01-17 써드 웨이브 테크놀로지스, 아이앤씨. 핵산의 검출
US20080131875A1 (en) * 2006-06-07 2008-06-05 Third Wave Technologies, Inc. Multiplex assays
US7759062B2 (en) * 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
US8080643B2 (en) * 2006-09-05 2011-12-20 Third Wave Technologies, Inc. HPV primers
EP2079754B1 (de) 2006-10-04 2017-03-15 Third Wave Technologies, Inc. Snap-back primer und nachweisbare hairpin-strukturen
PL2033641T3 (pl) 2007-08-01 2012-05-31 Ferlux Sposób wytwarzania ekstraktów bogatych w proantocyjanidyny
WO2009117327A2 (en) 2008-03-15 2009-09-24 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
JP2009268665A (ja) * 2008-05-07 2009-11-19 Canon Inc 吸入装置
EP2310522B1 (de) * 2008-06-17 2013-03-20 Sigma-Aldrich Co. LLC Echtzeit-polymerasekettenreaktionsprozess mit universellem nachweisverfahren
EP2853601B1 (de) 2008-07-18 2016-09-21 TrovaGene, Inc. Verfahren für den PCR-basierten Nachweis ultrakurzer Nukleinsäuresequenzen
US20110171649A1 (en) * 2008-09-10 2011-07-14 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
US9914963B2 (en) 2009-09-28 2018-03-13 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
US9617588B2 (en) 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
US8715937B2 (en) 2010-11-15 2014-05-06 Exact Sciences Corporation Mutation detection assay
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
JP5852222B2 (ja) 2011-03-29 2016-02-03 シージーン アイエヌシー Ｐｔｏ切断及び延長−依存的切断によるターゲット核酸配列の検出
MX342067B (es) 2011-05-04 2016-09-09 Seegene Inc Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda.
WO2012173905A1 (en) * 2011-06-16 2012-12-20 The Regents Of The Unversity Of California Salt-tolerant dna polymerases
EP2748339B1 (de) 2011-10-18 2017-09-27 Exact Sciences Corporation Test für den nachweis von multiplexierten kras-mutationen
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
EP2823061B1 (de) 2012-03-05 2018-02-14 Seegene, Inc. Detektion einer nucleotidvariation einer nucleinsäurezielsequenz durch pto-spaltung und extensionstest
WO2013187628A1 (en) * 2012-06-11 2013-12-19 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent transcription
EP2943586B1 (de) 2012-12-27 2017-09-20 Seegene, Inc. Erkennung von zielnukleinsäuresequenzen durch pto-spaltung und erweiterungsabhängiges nicht-hybridisierungs-assay
AU2014200958B2 (en) 2013-02-25 2016-01-14 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
WO2014158628A1 (en) 2013-03-14 2014-10-02 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules
CN105229176A (zh) 2013-03-15 2016-01-06 特罗瓦基因公司 尿液中核酸的检测
JP6050555B2 (ja) 2013-07-15 2016-12-21 シージーン アイエヌシー Ｐｔｏの切断及び延長−依存的固定化オリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出
EP3027772B1 (de) * 2013-07-31 2019-03-06 Seegene, Inc. Verfahren zur bestimmung eines snp-genotyps
KR101757473B1 (ko) 2013-10-18 2017-07-13 주식회사 씨젠 ｈＣＴＯ를 이용하는 ＰＴＯ 절단 및 연장 분석에 의한 고상에서의 타겟 핵산 서열 검출
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
WO2016179093A1 (en) 2015-05-01 2016-11-10 Gen-Probe Incorporated Multiplex invasive cleavage assays
CN108368547B (zh) 2015-12-15 2022-04-05 Seegene株式会社 与靶核酸序列有关的信号提取
WO2017213458A1 (en) 2016-06-10 2017-12-14 Seegene, Inc. Methods for preparing tagging oligonucleotides
ES2893411T3 (es) 2016-09-15 2022-02-09 Hoffmann La Roche Procedimientos para realizar PCR multiplexada
EP3523452B1 (de) 2016-10-06 2026-04-15 Seegene, Inc. Verfahren zur herstellung von oligonukleotiden zum nachweis von zielnukleinsäuremolekülen in proben
RU2665631C2 (ru) 2017-01-25 2018-09-03 Общество с ограниченной ответственностью "Секвойя Дженетикс" Способ специфической идентификации последовательностей днк
CN110475871B (zh) 2017-03-28 2024-04-12 Seegene株式会社 用于确定靶核酸序列的存在的分析信号
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
US10995104B2 (en) 2017-05-30 2021-05-04 Roche Molecular System, Inc. Catalysts for reversing formaldehyde adducts and crosslinks
WO2019026884A1 (ja) 2017-07-31 2019-02-07 凸版印刷株式会社 核酸検出方法および検出用試薬
US11859257B2 (en) 2017-08-11 2024-01-02 Gen-Probe Incorporated Compositions and methods for detecting Staphylococcus aureus
WO2019045532A2 (en) 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
ES2931536T3 (es) * 2017-08-31 2022-12-30 Somaprobes Sl Ensayo de flujo lateral para detectar la presencia de una célula de mamífero o bacterias específicas en una muestra biológica
JP7012151B2 (ja) 2017-09-28 2022-01-27 シージーン アイエヌシー 試料内ターゲット分析物を分析する方法及び装置
KR102345601B1 (ko) 2017-09-29 2021-12-30 주식회사 씨젠 Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출
WO2019118550A1 (en) 2017-12-13 2019-06-20 Gen-Probe Incorporated Methods for biological sample processing
EP3724354A1 (de) 2017-12-15 2020-10-21 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von toxigenem clostridium difficile
EP3735476B1 (de) 2018-01-05 2023-08-23 Seegene, Inc. Verfahren zur bestimmung der anwesenheit oder abwesenheit von m. tuberculosis, m. bovis und m. bovis bcg in einer probe
KR102464501B1 (ko) 2018-04-20 2022-11-17 주식회사 씨젠 샘플 내 복수의 타겟 핵산 서열의 검출을 위한 방법 및 장치
WO2020122679A1 (en) 2018-12-14 2020-06-18 Seegene, Inc. Method for detecting a target analyte in a sample using an s-shaped function for a slope data set
EP3914732A1 (de) 2019-01-25 2021-12-01 Gen-Probe Incorporated Nachweis von arzneimittelresistentem mycoplasma genitalium
EP4301880A4 (de) 2021-03-05 2025-06-18 Seegene, Inc. Verfahren zum nachweis von sars-cov-2-mutationen
KR20240058112A (ko) 2021-09-17 2024-05-03 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
CN120442758A (zh) 2021-09-30 2025-08-08 简·探针公司 可温度选择的fret盒信号传导
US20230348957A1 (en) 2021-12-07 2023-11-02 Luminex Corporation Methods and compositions for nucleic acid analysis
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers
EP4621068A1 (de) 2022-11-16 2025-09-24 Seegene, Inc. Nachweis von zielnukleinsäuren durch quenching-pto-spaltungs- und verlängerungs-(q-ptoce)-assay

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5011769A (en) * 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
JP2856804B2 (ja) * 1988-03-24 1999-02-10 ユニバーシティ オブ アイオワ リサーチ ファウンデイション 標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系
US5144019A (en) * 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
AU646430B2 (en) * 1989-12-22 1994-02-24 F. Hoffmann-La Roche Ag Recombinant expression vectors and purification methods for thermus thermophilus DNA polymerase
GB9009903D0 (en) * 1990-05-02 1990-06-27 Ici Plc Assay for specific nucleic acid sequences
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP2709311B2 (ja) * 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
US6083686A (en) * 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase

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