ATE290433T1 - Verfahren zur reinigung von proteinen oder mischungen von proteinen und nukleinsäuren und so geschaffene zusammesetzungen von partikeln und proteinen - Google Patents

Verfahren zur reinigung von proteinen oder mischungen von proteinen und nukleinsäuren und so geschaffene zusammesetzungen von partikeln und proteinen

Info

Publication number
ATE290433T1
ATE290433T1 AT01907667T AT01907667T ATE290433T1 AT E290433 T1 ATE290433 T1 AT E290433T1 AT 01907667 T AT01907667 T AT 01907667T AT 01907667 T AT01907667 T AT 01907667T AT E290433 T1 ATE290433 T1 AT E290433T1
Authority
AT
Austria
Prior art keywords
proteins
functional groups
mixtures
core
compositions
Prior art date
Application number
AT01907667T
Other languages
English (en)
Inventor
Abdelhamid Elaissari
Bernard Mandrand
Thierry Delair
Doran Spencer
Ahmed Arkis
Original Assignee
Bio Merieux
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux filed Critical Bio Merieux
Application granted granted Critical
Publication of ATE290433T1 publication Critical patent/ATE290433T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/5434—Magnetic particles using magnetic particle immunoreagent carriers which constitute new materials per se

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Soft Magnetic Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AT01907667T 2000-01-21 2001-01-22 Verfahren zur reinigung von proteinen oder mischungen von proteinen und nukleinsäuren und so geschaffene zusammesetzungen von partikeln und proteinen ATE290433T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0000862A FR2804117B1 (fr) 2000-01-21 2000-01-21 Procede d'isolement de proteines et/ou d'acides nucleiques, complexes de particules et de proteines et/ou d'acides nucleiques, reactif et applications
PCT/FR2001/000205 WO2001052612A2 (fr) 2000-01-21 2001-01-22 Procede d'isolement de proteines ou d'associations de proteines et d'acides nucleiques et complexes de particules et de proteines ainsi formes

Publications (1)

Publication Number Publication Date
ATE290433T1 true ATE290433T1 (de) 2005-03-15

Family

ID=8846236

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01907667T ATE290433T1 (de) 2000-01-21 2001-01-22 Verfahren zur reinigung von proteinen oder mischungen von proteinen und nukleinsäuren und so geschaffene zusammesetzungen von partikeln und proteinen

Country Status (9)

Country Link
US (3) US7060804B2 (de)
EP (1) EP1248679B1 (de)
AT (1) ATE290433T1 (de)
AU (1) AU2001235580A1 (de)
CA (1) CA2412536A1 (de)
DE (1) DE60109272T2 (de)
ES (1) ES2237554T3 (de)
FR (1) FR2804117B1 (de)
WO (1) WO2001052612A2 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
DE10256892A1 (de) * 2002-11-29 2004-06-09 Henkel Kgaa Neue magnetische Partikel zur Abtrennung von Mikroorganismen aus technischen Medien
US20070003975A1 (en) * 2003-05-02 2007-01-04 Canon Kabushiki Kaisha Structured construct and producing method therefor
EP1664110B1 (de) * 2003-08-26 2008-08-20 Danmarks Tekniske Universitet Kontinuierliches verfahren zur anordnung von makromolekulären substanzen und die anschliessende aufnahme und isolierung einer makromolekulären anordnung, sowie ein für dieses verfahren geeignetes system
JP2007505311A (ja) * 2003-09-09 2007-03-08 コニンクリユケ フィリップス エレクトロニクス エヌ．ブイ． 検体を検出するためのナノ粒子
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
CN1861628B (zh) * 2005-05-11 2010-05-05 上海师范大学 一种分离中药蛋白质的方法
JP4716034B2 (ja) * 2006-03-24 2011-07-06 Ｊｓｒ株式会社 磁性粒子およびその製造方法
US8163886B2 (en) 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US7981688B2 (en) 2007-03-08 2011-07-19 University Of Washington Stimuli-responsive magnetic nanoparticles and related methods
AU2008230240B2 (en) 2007-03-26 2012-09-13 Academisch Ziekenhuis Leiden H.O.D.N. Lumc PRAME derived peptides and immunogenic compositions comprising these
US20100018674A1 (en) * 2008-07-22 2010-01-28 Donald John Enzinna Reservoir with moveable partition for quick recovery
JP5326443B2 (ja) * 2008-09-05 2013-10-30 Ｊｎｃ株式会社 凍結乾燥可能な温度応答性磁性微粒子
WO2010074953A1 (en) 2008-12-16 2010-07-01 Millipore Corporation Stirred tank reactor and method
US8426214B2 (en) * 2009-06-12 2013-04-23 University Of Washington System and method for magnetically concentrating and detecting biomarkers
US9080933B2 (en) 2009-11-09 2015-07-14 University Of Washington Through Its Center For Commercialization Stimuli-responsive polymer diagnostic assay comprising magnetic nanoparticles and capture conjugates
US20110117668A1 (en) * 2009-11-09 2011-05-19 University Of Washington Through Its Center For Commercialization Self-powered smart diagnostic devices
DK2571903T3 (da) 2010-05-17 2019-11-04 Emd Millipore Corp Polymerer, der reagerer på stimuli, til oprensning af biomolekyler
US20120288866A1 (en) * 2011-05-11 2012-11-15 Genturadx, Inc. Systems and methods for producing an evaporation barrier in a reaction chamber
EP2971188A1 (de) * 2013-03-15 2016-01-20 The U.S.A. as represented by the Secretary, Department of Health and Human Services Selektiver nachweis von hepatitis a-, b-, c-, d- oder e-viren oder kombinationen davon
DE102016121483B4 (de) * 2016-11-09 2020-06-18 Axagarius Gmbh & Co. Kg Partikelförmiges Feststoff-Kompositmaterial zur Nukleinsäureaufreinigung, enthaltend magnetische Nanopartikel , Verfahren zu dessen Herstellung und dessen Verwendung

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4018886A (en) * 1975-07-01 1977-04-19 General Electric Company Diagnostic method and device employing protein-coated magnetic particles
DE3517333A1 (de) 1985-05-14 1986-11-20 Basf Ag, 6700 Ludwigshafen Verfahren zur herstellung stabiler dispersionen feinteiliger polyisocyanate und deren verwendung
US5051469A (en) 1985-12-13 1991-09-24 Monsanto Company Rubber modified reaction molded nylon-6 block copolymers
WO1989004373A1 (en) * 1987-10-26 1989-05-18 Baxter International Inc. Process for producing magnetically responsive polymer particles and application thereof
US5049469A (en) 1989-12-27 1991-09-17 Eastman Kodak Company Toner image pressure transfer method and toner useful therefor
FR2707010B1 (de) * 1993-06-25 1995-09-29 Bio Merieux
US5756273A (en) 1996-02-06 1998-05-26 Eastman Kodak Company Photographic element containing a core/shell polymer latex
US5976382A (en) 1996-07-10 1999-11-02 Ligochem, Inc. Removal of proteins from aqueuos media by precipitation
FR2762394B1 (fr) * 1997-04-16 1999-05-28 Bio Merieux Compose ligand de coordination et utilisation pour fixer un materiel biologique
AU7685498A (en) * 1997-05-14 1998-12-08 Weixin Tang Fully-coated, uniform-sized metallic particles
FR2767137A1 (fr) * 1997-08-07 1999-02-12 Bio Merieux Compose chimique complexe, synthese et applications diverses dudit compose
FR2773416B1 (fr) * 1998-01-06 2000-02-11 Bio Merieux Particules magnetiques perfectionnees, leurs procedes d'obtention et leurs utilisations dans la separation de molecules
DE19803098A1 (de) 1998-01-28 1999-07-29 Basf Ag Verfahren zur Herstellung eines partikelförmigen Polymerisates
FR2800636A1 (fr) * 1999-11-05 2001-05-11 Bio Merieux Particules composites, conjugues derives, leur procede de preparation et utilisations
FR2800635B1 (fr) * 1999-11-05 2002-07-26 Bio Merieux Nanospheres composites, conjugues derives, procede de preparation et leurs utilisations

Also Published As

Publication number Publication date
US8093026B2 (en) 2012-01-10
CA2412536A1 (fr) 2001-07-26
DE60109272T2 (de) 2006-04-13
US20100209987A1 (en) 2010-08-19
DE60109272D1 (de) 2005-04-14
WO2001052612A3 (fr) 2002-03-07
US7060804B2 (en) 2006-06-13
FR2804117B1 (fr) 2004-08-20
US7700744B2 (en) 2010-04-20
EP1248679A2 (de) 2002-10-16
FR2804117A1 (fr) 2001-07-27
US20060105326A1 (en) 2006-05-18
WO2001052612A2 (fr) 2001-07-26
US20030175691A1 (en) 2003-09-18
EP1248679B1 (de) 2005-03-09
AU2001235580A1 (en) 2001-07-31
ES2237554T3 (es) 2005-08-01

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