ATE491031T1 - Thermostabile chimäre nukleinsäurepolymerasen und ihre verwendungen - Google Patents

Thermostabile chimäre nukleinsäurepolymerasen und ihre verwendungen

Info

Publication number
ATE491031T1
ATE491031T1 AT08151710T AT08151710T ATE491031T1 AT E491031 T1 ATE491031 T1 AT E491031T1 AT 08151710 T AT08151710 T AT 08151710T AT 08151710 T AT08151710 T AT 08151710T AT E491031 T1 ATE491031 T1 AT E491031T1
Authority
AT
Austria
Prior art keywords
nucleic acid
chimeric nucleic
polymerases
acid polymerases
thermostable
Prior art date
Application number
AT08151710T
Other languages
English (en)
Inventor
Dirk Loeffert
Andreas Missel
Jie Kang
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE491031T1 publication Critical patent/ATE491031T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)
  • Saccharide Compounds (AREA)
AT08151710T 2000-02-17 2001-02-16 Thermostabile chimäre nukleinsäurepolymerasen und ihre verwendungen ATE491031T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50615300A 2000-02-17 2000-02-17

Publications (1)

Publication Number Publication Date
ATE491031T1 true ATE491031T1 (de) 2010-12-15

Family

ID=24013413

Family Applications (2)

Application Number Title Priority Date Filing Date
AT08151710T ATE491031T1 (de) 2000-02-17 2001-02-16 Thermostabile chimäre nukleinsäurepolymerasen und ihre verwendungen
AT01953004T ATE395420T1 (de) 2000-02-17 2001-02-16 Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT01953004T ATE395420T1 (de) 2000-02-17 2001-02-16 Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen

Country Status (5)

Country Link
US (2) US8637288B2 (de)
EP (2) EP1925669B1 (de)
AT (2) ATE491031T1 (de)
DE (2) DE60143632D1 (de)
WO (1) WO2001061015A2 (de)

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ATE491031T1 (de) 2000-02-17 2010-12-15 Qiagen Gmbh Thermostabile chimäre nukleinsäurepolymerasen und ihre verwendungen
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
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US7560260B2 (en) 2002-07-25 2009-07-14 Bio-Rad Laboratories, Inc. Compositions with polymerase activity
ATE486931T1 (de) * 2002-07-25 2010-11-15 Bio Rad Laboratories Hybridpolymerase-verfahren und -zusammensetzungen
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
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US8652782B2 (en) * 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US20140038174A1 (en) * 2011-04-26 2014-02-06 Longhorn Vaccines And Diagnostics, Llc Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples
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US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
CA2742593C (en) 2008-11-03 2020-05-05 Kapa Biosystems, Inc. Chimeric dna polymerases
EP2430195B1 (de) * 2009-05-11 2019-01-23 Biomatrica, INC. Zusammensetzungen und verfahren zur lagerung biologischer proben
WO2012018639A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
EP2598660B1 (de) 2010-07-26 2017-03-15 Biomatrica, INC. Zusammensetzungen zur stabilisierung von dna, rna und proteinen in blut- und anderen biologischen proben während des transports und der lagerung bei umgebungstemperaturen
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
US10023856B2 (en) * 2013-09-25 2018-07-17 Thermo Fisher Scientific Baltics Uab Enzyme composition for DNA end repair, adenylation, phosphorylation
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ES2786373T3 (es) 2014-06-10 2020-10-09 Biomatrica Inc Estabilización de trombocitos a temperaturas ambiente
CN108368151B (zh) 2015-12-07 2022-11-15 生物辐射实验室股份有限公司 二聚逆转录酶
KR20250047404A (ko) 2015-12-08 2025-04-03 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
US20240200040A1 (en) * 2019-12-23 2024-06-20 Bgi Shenzhen Chimeric dna polymerase and application thereof
CN117050967B (zh) * 2023-08-21 2024-04-09 南京诺唯赞生物科技股份有限公司 一种改善二代测序文库gc均衡性的方法

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US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
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Also Published As

Publication number Publication date
US8637288B2 (en) 2014-01-28
EP1925669A1 (de) 2008-05-28
DE60143632D1 (de) 2011-01-20
EP1259624A2 (de) 2002-11-27
EP1925669B1 (de) 2010-12-08
WO2001061015A2 (de) 2001-08-23
WO2001061015A3 (de) 2002-04-25
US20030138805A1 (en) 2003-07-24
US20140154748A1 (en) 2014-06-05
DE60133992D1 (de) 2008-06-26
ATE395420T1 (de) 2008-05-15
EP1259624B1 (de) 2008-05-14

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