ATE302270T1 - Mutation von dna polymerasen aus archaeobakterien - Google Patents

Mutation von dna polymerasen aus archaeobakterien

Info

Publication number
ATE302270T1
ATE302270T1 AT03746856T AT03746856T ATE302270T1 AT E302270 T1 ATE302270 T1 AT E302270T1 AT 03746856 T AT03746856 T AT 03746856T AT 03746856 T AT03746856 T AT 03746856T AT E302270 T1 ATE302270 T1 AT E302270T1
Authority
AT
Austria
Prior art keywords
polymerase
wild
variant
type
archaeobacteria
Prior art date
Application number
AT03746856T
Other languages
English (en)
Inventor
Bernard Connolly
Mark Fogg
Laurence Pearl
Original Assignee
Univ Newcastle
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Newcastle filed Critical Univ Newcastle
Application granted granted Critical
Publication of ATE302270T1 publication Critical patent/ATE302270T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT03746856T 2002-04-17 2003-04-15 Mutation von dna polymerasen aus archaeobakterien ATE302270T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0208768.2A GB0208768D0 (en) 2002-04-17 2002-04-17 DNA polymerases
PCT/GB2003/001623 WO2003089637A1 (en) 2002-04-17 2003-04-15 Mutation of dna polymerases from archaeobacteria

Publications (1)

Publication Number Publication Date
ATE302270T1 true ATE302270T1 (de) 2005-09-15

Family

ID=9934981

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03746856T ATE302270T1 (de) 2002-04-17 2003-04-15 Mutation von dna polymerasen aus archaeobakterien

Country Status (10)

Country Link
US (1) US8557554B2 (de)
EP (1) EP1463809B1 (de)
JP (1) JP4395377B2 (de)
AT (1) ATE302270T1 (de)
AU (1) AU2003226543B2 (de)
CA (1) CA2475418C (de)
DE (1) DE60301325T2 (de)
DK (1) DK1463809T3 (de)
GB (1) GB0208768D0 (de)
WO (1) WO2003089637A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004038007A2 (en) * 2002-10-25 2004-05-06 Stratagene Dna polymerases with reduced base analog detection activity
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
CN101368214B (zh) * 2008-09-24 2010-09-15 上海大学 用于古细菌多样性分析的针对16S rRNA基因的引物
JP5258512B2 (ja) * 2008-10-30 2013-08-07 株式会社日立製作所 エキソヌクレアーゼ活性増強ｄｎａポリメラーゼ変異体
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
US20130040365A1 (en) * 2011-08-10 2013-02-14 Life Technologies Corporation Polymerase compositions, methods of making and using same
JP2014000015A (ja) * 2012-06-15 2014-01-09 Kaneka Corp 核酸解析法
JP6478444B2 (ja) * 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
JP6428997B2 (ja) * 2013-08-06 2018-11-28 東洋紡株式会社 核酸増幅法
WO2015019952A1 (ja) * 2013-08-06 2015-02-12 東洋紡株式会社 核酸増幅法
CN105452451B (zh) 2013-12-06 2020-06-05 生物辐射实验室股份有限公司 融合聚合酶
EP3307908B1 (de) 2015-06-09 2019-09-11 Life Technologies Corporation Verfahren zur molekularen markierung
EP3356557B1 (de) 2015-10-01 2022-07-20 Life Technologies Corporation Polymerasezusammensetzungen und kits sowie verfahren zur verwendung und herstellung davon
WO2017121836A1 (en) 2016-01-15 2017-07-20 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
EP3580351A1 (de) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymeraseenzym aus 9°n
EP3645710A1 (de) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophile dna-polymerase-mutanten
CN112899353A (zh) * 2021-01-05 2021-06-04 南京普济生物有限公司 一种用于dna聚合酶识别特异性的缓冲液及其应用
CA3246669A1 (en) 2023-01-06 2024-07-11 Illumina, Inc. URACIL REDUCTION BY POLYMERASE
CN116083390B (zh) * 2023-02-06 2026-03-31 苏州金唯智生物科技有限公司 一种dna聚合酶突变体及其制备方法和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827716A (en) * 1996-07-30 1998-10-27 Amersham Life Science, Inc. Modified Pol-II type DNA polymerases
ATE286981T1 (de) 1999-09-28 2005-01-15 Roche Diagnostics Gmbh Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation
WO2001038546A1 (en) 1999-11-23 2001-05-31 Amersham Biosciences Corp Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus

Also Published As

Publication number Publication date
GB0208768D0 (en) 2002-05-29
DK1463809T3 (da) 2005-12-19
WO2003089637A1 (en) 2003-10-30
JP2005526510A (ja) 2005-09-08
DE60301325D1 (de) 2005-09-22
AU2003226543B2 (en) 2004-11-04
CA2475418A1 (en) 2003-10-30
US8557554B2 (en) 2013-10-15
EP1463809A1 (de) 2004-10-06
JP4395377B2 (ja) 2010-01-06
DE60301325T2 (de) 2006-05-24
EP1463809B1 (de) 2005-08-17
US20060057682A1 (en) 2006-03-16
AU2003226543A1 (en) 2003-11-03
CA2475418C (en) 2013-06-11

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