ATE317024T1 - Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden - Google Patents
Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurdenInfo
- Publication number
- ATE317024T1 ATE317024T1 AT00991364T AT00991364T ATE317024T1 AT E317024 T1 ATE317024 T1 AT E317024T1 AT 00991364 T AT00991364 T AT 00991364T AT 00991364 T AT00991364 T AT 00991364T AT E317024 T1 ATE317024 T1 AT E317024T1
- Authority
- AT
- Austria
- Prior art keywords
- dsdna
- iis
- restriction
- restricted
- detecting
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 2
- 239000012634 fragment Substances 0.000 title abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 13
- 102000053602 DNA Human genes 0.000 abstract 13
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 abstract 2
- 238000004128 high performance liquid chromatography Methods 0.000 abstract 2
- 108091008146 restriction endonucleases Proteins 0.000 abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000004949 mass spectrometry Methods 0.000 abstract 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/313—Type II endonucleases, i.e. cutting outside recognition site
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99204614 | 1999-12-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE317024T1 true ATE317024T1 (de) | 2006-02-15 |
Family
ID=8241132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00991364T ATE317024T1 (de) | 1999-12-29 | 2000-12-28 | Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7166429B2 (de) |
| EP (1) | EP1242630B1 (de) |
| AT (1) | ATE317024T1 (de) |
| AU (1) | AU3246601A (de) |
| DE (1) | DE60025850T2 (de) |
| WO (1) | WO2001049882A2 (de) |
Families Citing this family (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPR221400A0 (en) * | 2000-12-20 | 2001-01-25 | Murdoch Childrens Research Institute, The | Diagnostic assay |
| US20030027135A1 (en) * | 2001-03-02 | 2003-02-06 | Ecker David J. | Method for rapid detection and identification of bioagents |
| WO2004060278A2 (en) | 2002-12-06 | 2004-07-22 | Isis Pharmaceuticals, Inc. | Methods for rapid identification of pathogens in humans and animals |
| US20040121309A1 (en) | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues |
| US7226739B2 (en) | 2001-03-02 | 2007-06-05 | Isis Pharmaceuticals, Inc | Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations |
| US7718354B2 (en) | 2001-03-02 | 2010-05-18 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
| US20040121314A1 (en) * | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in containers |
| US7666588B2 (en) | 2001-03-02 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy |
| US7217510B2 (en) | 2001-06-26 | 2007-05-15 | Isis Pharmaceuticals, Inc. | Methods for providing bacterial bioagent characterizing information |
| US8073627B2 (en) | 2001-06-26 | 2011-12-06 | Ibis Biosciences, Inc. | System for indentification of pathogens |
| FR2832730A1 (fr) * | 2001-11-26 | 2003-05-30 | Commissariat Energie Atomique | Puce a adn universelle, son procede de fabrication, et utilisations de la puce |
| KR20050008651A (ko) * | 2002-03-05 | 2005-01-21 | 솔렉사 리미티드 | 표현형과 관련된 게놈 와이드 서열 변형을 검출하는 방법 |
| GB0228614D0 (en) * | 2002-12-07 | 2003-01-15 | Fu Guoliang | Oligonucleotide guided analysis of gene expression |
| FR2852605B1 (fr) * | 2003-03-18 | 2012-11-30 | Commissariat Energie Atomique | Procede de preparation de fragments d'adn et ses applications |
| US8046171B2 (en) | 2003-04-18 | 2011-10-25 | Ibis Biosciences, Inc. | Methods and apparatus for genetic evaluation |
| US8057993B2 (en) | 2003-04-26 | 2011-11-15 | Ibis Biosciences, Inc. | Methods for identification of coronaviruses |
| US7964343B2 (en) | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US8546082B2 (en) | 2003-09-11 | 2013-10-01 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
| US8097416B2 (en) | 2003-09-11 | 2012-01-17 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
| US20120122102A1 (en) | 2003-09-11 | 2012-05-17 | Rangarajan Sampath | Compositions for use in identification of bacteria |
| US8163895B2 (en) | 2003-12-05 | 2012-04-24 | Ibis Biosciences, Inc. | Compositions for use in identification of orthopoxviruses |
| US7666592B2 (en) | 2004-02-18 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for concurrent identification and quantification of an unknown bioagent |
| US8119336B2 (en) | 2004-03-03 | 2012-02-21 | Ibis Biosciences, Inc. | Compositions for use in identification of alphaviruses |
| JP4810533B2 (ja) | 2004-05-24 | 2011-11-09 | アイビス バイオサイエンシズ インコーポレイティッド | ディジタルスレショルド化による選択的イオン濾過作用を用いた質量分光測定法 |
| US20050266411A1 (en) | 2004-05-25 | 2005-12-01 | Hofstadler Steven A | Methods for rapid forensic analysis of mitochondrial DNA |
| US7811753B2 (en) | 2004-07-14 | 2010-10-12 | Ibis Biosciences, Inc. | Methods for repairing degraded DNA |
| US8084207B2 (en) | 2005-03-03 | 2011-12-27 | Ibis Bioscience, Inc. | Compositions for use in identification of papillomavirus |
| US20060205040A1 (en) | 2005-03-03 | 2006-09-14 | Rangarajan Sampath | Compositions for use in identification of adventitious viruses |
| CA2612145A1 (en) * | 2005-05-10 | 2006-11-16 | Eric A. Johnson | Methods of mapping polymorphisms and polymorphism microarrays |
| GB0514910D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Method for sequencing a polynucleotide template |
| GB0514935D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Methods for sequencing a polynucleotide template |
| US8026084B2 (en) | 2005-07-21 | 2011-09-27 | Ibis Biosciences, Inc. | Methods for rapid identification and quantitation of nucleic acid variants |
| EP1987159B2 (de) | 2006-02-08 | 2020-08-12 | Illumina Cambridge Limited | Verfahren zur sequenzierung einer polynukleotidmatrize |
| US20080131937A1 (en) * | 2006-06-22 | 2008-06-05 | Applera Corporation | Conversion of Target Specific Amplification to Universal Sequencing |
| WO2008143627A2 (en) | 2006-09-14 | 2008-11-27 | Ibis Biosciences, Inc. | Targeted whole genome amplification method for identification of pathogens |
| US7754429B2 (en) | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
| JP5680304B2 (ja) | 2007-02-23 | 2015-03-04 | アイビス バイオサイエンシズ インコーポレイティッド | 迅速な法医学的ｄｎａ分析法 |
| US9598724B2 (en) | 2007-06-01 | 2017-03-21 | Ibis Biosciences, Inc. | Methods and compositions for multiple displacement amplification of nucleic acids |
| US9624489B2 (en) | 2008-03-10 | 2017-04-18 | Illumina, Inc. | Methods for selecting and amplifying polynucleotides |
| US8999642B2 (en) | 2008-03-10 | 2015-04-07 | Illumina, Inc. | Methods for selecting and amplifying polynucleotides |
| ES2528971T3 (es) * | 2008-03-17 | 2015-02-13 | Stichting Genetwister Ip | Descubrimientos de genes vinculados a la expresión |
| JP2011515102A (ja) | 2008-03-28 | 2011-05-19 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | 核酸シーケンシング用組成物及び方法 |
| WO2009120374A2 (en) | 2008-03-28 | 2009-10-01 | Pacific Biosciences Of California, Inc. | Methods and compositions for nucleic acid sample preparation |
| EP2349549B1 (de) | 2008-09-16 | 2012-07-18 | Ibis Biosciences, Inc. | Mischpatronen, mischstationen und verwandte ausrüstungen, und system |
| EP2347254A2 (de) | 2008-09-16 | 2011-07-27 | Ibis Biosciences, Inc. | Probenverarbeitungseinheiten, -systeme und damit verbundene verfahren |
| EP2344893B1 (de) | 2008-09-16 | 2014-10-15 | Ibis Biosciences, Inc. | Mikroplatten-handhabungssysteme und verfahren |
| EP2396803A4 (de) | 2009-02-12 | 2016-10-26 | Ibis Biosciences Inc | Ionisationssondenanordnungen |
| WO2011008972A1 (en) | 2009-07-17 | 2011-01-20 | Ibis Biosciences, Inc. | Systems for bioagent identification |
| US8950604B2 (en) | 2009-07-17 | 2015-02-10 | Ibis Biosciences, Inc. | Lift and mount apparatus |
| US9890408B2 (en) | 2009-10-15 | 2018-02-13 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| CN102776172B (zh) * | 2012-06-13 | 2013-08-07 | 山东农业大学 | 一种通用多重pcr方法 |
| CN104232771B (zh) * | 2014-09-12 | 2016-09-07 | 吉林大学 | 一种选择性扩增孕妇血浆中游离胎儿dna的方法 |
| CN114350752B (zh) | 2015-07-17 | 2025-02-25 | 布鲁克空间生物学公司 | 在横切面组织的用户定义区域中的基因表达的同时定量 |
| CN109563543B (zh) | 2016-07-15 | 2022-08-30 | 加利福尼亚大学董事会 | 产生核酸库的方法 |
| US11584929B2 (en) | 2018-01-12 | 2023-02-21 | Claret Bioscience, Llc | Methods and compositions for analyzing nucleic acid |
| JP7372927B6 (ja) * | 2018-02-12 | 2023-11-27 | ナノストリング テクノロジーズ，インコーポレイティド | 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法 |
| WO2019236726A1 (en) | 2018-06-06 | 2019-12-12 | The Regents Of The University Of California | Methods of producing nucleic acid libraries and compositions and kits for practicing same |
| AU2019287163B2 (en) * | 2018-06-12 | 2025-08-21 | Keygene N.V. | Nucleic acid amplification method |
| CN119757294B (zh) * | 2024-12-20 | 2025-08-29 | 厦门市亮惠诊断技术有限公司 | 一种用于d-乳酸高灵敏检测方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5518900A (en) | 1993-01-15 | 1996-05-21 | Molecular Tool, Inc. | Method for generating single-stranded DNA molecules |
| ZA927323B (en) | 1991-09-24 | 1993-08-30 | Keygene Nv | Selective restriction fragment amplification: A general method for DNA fingerprinting |
| US5710000A (en) * | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| US6051378A (en) | 1996-03-04 | 2000-04-18 | Genetrace Systems Inc. | Methods of screening nucleic acids using mass spectrometry |
| AU4174397A (en) * | 1996-08-30 | 1998-03-19 | Life Technologies, Inc. | Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna |
| US6461814B1 (en) * | 1997-01-15 | 2002-10-08 | Dominic G. Spinella | Method of identifying gene transcription patterns |
| US6306593B1 (en) * | 1998-09-17 | 2001-10-23 | Union Camp Corporation | Selective AFLP primers for reduction of complexity of maker genotyping and DNA markers for loblolly pine identified using the AFLP primers |
| IL143486A0 (en) * | 1998-12-04 | 2002-04-21 | Keygene Nv | Array and method for analysing nucleic acid sequences |
-
2000
- 2000-12-28 DE DE60025850T patent/DE60025850T2/de not_active Expired - Lifetime
- 2000-12-28 AU AU32466/01A patent/AU3246601A/en not_active Abandoned
- 2000-12-28 AT AT00991364T patent/ATE317024T1/de not_active IP Right Cessation
- 2000-12-28 EP EP00991364A patent/EP1242630B1/de not_active Expired - Lifetime
- 2000-12-28 WO PCT/NL2000/000963 patent/WO2001049882A2/en not_active Ceased
- 2000-12-28 US US10/169,371 patent/US7166429B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU3246601A (en) | 2001-07-16 |
| DE60025850T2 (de) | 2006-08-03 |
| DE60025850D1 (de) | 2006-04-13 |
| EP1242630B1 (de) | 2006-02-01 |
| US7166429B2 (en) | 2007-01-23 |
| WO2001049882A3 (en) | 2002-02-07 |
| US20030175729A1 (en) | 2003-09-18 |
| EP1242630A2 (de) | 2002-09-25 |
| WO2001049882A2 (en) | 2001-07-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |