ATE321871T1 - Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten - Google Patents
Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryotenInfo
- Publication number
- ATE321871T1 ATE321871T1 AT96921541T AT96921541T ATE321871T1 AT E321871 T1 ATE321871 T1 AT E321871T1 AT 96921541 T AT96921541 T AT 96921541T AT 96921541 T AT96921541 T AT 96921541T AT E321871 T1 ATE321871 T1 AT E321871T1
- Authority
- AT
- Austria
- Prior art keywords
- tta
- tetracycline
- expression
- ttak
- autoregulatory
- Prior art date
Links
- 230000014509 gene expression Effects 0.000 title abstract 6
- 241000206602 Eukaryota Species 0.000 title 1
- 239000004098 Tetracycline Substances 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 229960002180 tetracycline Drugs 0.000 abstract 4
- 229930101283 tetracycline Natural products 0.000 abstract 4
- 235000019364 tetracycline Nutrition 0.000 abstract 4
- 150000003522 tetracyclines Chemical class 0.000 abstract 4
- 230000001042 autoregulative effect Effects 0.000 abstract 3
- 108060001084 Luciferase Proteins 0.000 abstract 2
- 239000005089 Luciferase Substances 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 1
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
- C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/474,169 US5851796A (en) | 1995-06-07 | 1995-06-07 | Autoregulatory tetracycline-regulated system for inducible gene expression in eucaryotes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE321871T1 true ATE321871T1 (de) | 2006-04-15 |
Family
ID=23882456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96921541T ATE321871T1 (de) | 1995-06-07 | 1996-06-07 | Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5851796A (de) |
| EP (1) | EP0832254B1 (de) |
| JP (1) | JPH11507539A (de) |
| AT (1) | ATE321871T1 (de) |
| AU (1) | AU6274596A (de) |
| DE (1) | DE69635992T2 (de) |
| WO (1) | WO1996040946A1 (de) |
Families Citing this family (64)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997020943A1 (en) * | 1995-12-01 | 1997-06-12 | Introgene B.V. | Regulated protein expression in stably transfected mammalian cells |
| US5891718A (en) * | 1996-03-27 | 1999-04-06 | Vical Incorporated | Tetracycline inducible/repressible systems |
| DE19635568C1 (de) * | 1996-09-02 | 1998-03-26 | Gsf Forschungszentrum Umwelt | Vektorsysteme zur konditionalen Genexpression |
| WO1998037185A2 (en) * | 1997-02-20 | 1998-08-27 | The Board Of Regents Of The University Of Texas System | Vectors for controlled gene expression |
| US6087166A (en) * | 1997-07-03 | 2000-07-11 | Basf Aktiengesellschaft | Transcriptional activators with graded transactivation potential |
| US6878516B1 (en) | 1998-01-16 | 2005-04-12 | Pharmacia & Upjohn Company | Autoregulatory system for validating microbial genes as possible antimicrobial targets using a tetracycline-controllable element |
| AU2312699A (en) * | 1998-01-16 | 1999-08-02 | Pharmacia & Upjohn Company | An autoregulatory system for validating microbial genes as possible antimicrobial targets using tetracycline-controllable element |
| US7037663B2 (en) * | 1998-02-19 | 2006-05-02 | Eastern Virginia Medical School | Human zona pellucida protein 3 and uses thereof |
| ATE284443T1 (de) * | 1998-02-19 | 2004-12-15 | Eastern Virginia Med School | Rekombinantes aktives humänes zona pellucida protein 3 (hzp3) |
| DE19831420A1 (de) * | 1998-07-14 | 2000-01-20 | Hoechst Marion Roussel De Gmbh | Expressionssysteme enthaltend chimäre Promotoren mit Bindungsstellen für rekombinante Transkriptionsfaktoren |
| US20050037335A1 (en) * | 1998-11-07 | 2005-02-17 | Wolfgang Hillen | Method for identifying novel transcriptional regulatory proteins |
| US6706491B1 (en) | 1999-04-09 | 2004-03-16 | The Board Of Trustees Of The University Of Illinois | Reagents and methods for identifying and modulating expression of genes regulated by p21 |
| CA2376665C (en) * | 1999-06-07 | 2014-02-25 | Wolfgang Hillen | Novel tet repressor-based transcriptional regulatory proteins |
| GB2355459B (en) * | 1999-11-29 | 2001-09-26 | Isis Innovation | A dominant conditional lethal genetic system |
| JP2005516586A (ja) * | 2001-07-20 | 2005-06-09 | ボード オブ トラスティーズ オブ ザ ユニヴァースティ オブ イリノイ | 癌の処置に対する遺伝子標的を同定するための試薬および方法 |
| US7148021B2 (en) * | 2001-08-02 | 2006-12-12 | Eastern Virginia Meical School | Human zona pellucida proteins and methods of their use in diagnosing male infertility |
| GB2402675B (en) * | 2003-05-12 | 2008-02-20 | Oxitec Ltd | Resistance dilution |
| GB2403475B (en) * | 2003-07-01 | 2008-02-06 | Oxitec Ltd | Stable integrands |
| GB2404382B (en) | 2003-07-28 | 2008-01-30 | Oxitec Ltd | Pest control |
| US8507277B2 (en) | 2003-10-24 | 2013-08-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides |
| US8133733B2 (en) | 2003-10-24 | 2012-03-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to target tissues |
| US8062891B2 (en) | 2003-10-24 | 2011-11-22 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to plants |
| US20090123468A1 (en) | 2003-10-24 | 2009-05-14 | Gencia Corporation | Transducible polypeptides for modifying metabolism |
| US8039587B2 (en) | 2003-10-24 | 2011-10-18 | Gencia Corporation | Methods and compositions for delivering polynucleotides |
| JP2009502180A (ja) | 2005-08-03 | 2009-01-29 | アデレイド リサーチ アンド イノベーション ピーティーワイ リミテッド | 多糖シンターゼ |
| WO2007091099A1 (en) * | 2006-02-10 | 2007-08-16 | Oxitec Limited | Gene expression system using alternative splicing in insects |
| GB2443186A (en) | 2006-10-25 | 2008-04-30 | Oxitec Ltd | Expression system for mediating alternative splicing |
| WO2008064056A2 (en) * | 2006-11-22 | 2008-05-29 | Pioneer Hi-Bred International, Inc. | Tetracycline repressor and uses thereof |
| MX2009011851A (es) | 2007-05-02 | 2010-03-04 | Merial Ltd | Plasmidos de dna que tienen expresion y estabilidad mejoradas. |
| EP2364322A1 (de) | 2008-10-28 | 2011-09-14 | Pioneer Hi-Bred International Inc. | Sulfonylharnstoffreaktive repressorproteine |
| BRPI0922735A2 (pt) | 2008-12-10 | 2017-06-13 | Synthetic Genomics Inc | produção de alcoóis de cadeia ramificada através de micro-organismos fotossintéticos. |
| US8956834B2 (en) | 2009-06-29 | 2015-02-17 | Synthetic Genomics, Inc. | Acyl-ACP thioesterase genes and uses therefor |
| US8633002B2 (en) | 2009-08-11 | 2014-01-21 | Synthetic Genomics, Inc. | Microbial production of fatty alcohols |
| WO2011029013A2 (en) | 2009-09-04 | 2011-03-10 | President And Fellows Of Harvard College | Production of secreted bioproducts from photosynthetic microbes |
| EP2655625B1 (de) | 2010-12-23 | 2017-01-18 | ExxonMobil Research and Engineering Company | Polypeptide mit lipolytischer aktivität, rekombinante mikroorganismen mit nukleinsäuren die für ein exogenes lipolytisches polypeptid kodieren und herstellung von fettsäuren und fettsäurederivaten |
| US8530207B2 (en) | 2010-12-23 | 2013-09-10 | Exxonmobil Research And Engineering Company | Photosynthetic microorganisms comprising exogenous prokaryotic acyl-ACP thioesterases and methods for producing fatty acids |
| ES2665516T3 (es) | 2010-12-23 | 2018-04-26 | Exxonmobil Research And Engineering Company | Microorganismos modificados genéticamente que comprenden 4-hidroxibenzoíl-CoA-tioesterasas, y métodos de uso de los mismos para producir ácidos grasos libres, derivados de ácidos grasos y 4-hidroxibenzoíl-CoA-tioesterasas |
| US8940508B2 (en) | 2010-12-31 | 2015-01-27 | Exxonmobil Research And Engineering Company | Enhancement of biomass production by disruption of light energy dissipation pathways |
| US9040264B2 (en) | 2011-03-31 | 2015-05-26 | Exxonmobil Research And Engineering Company | Recombinant cyanobacterium expressing a transcription factor domain protein |
| WO2012170436A1 (en) | 2011-06-06 | 2012-12-13 | The Regents Of The University Of California | Synthetic biology tools |
| BR112013033366A2 (pt) | 2011-06-30 | 2017-01-31 | Exxonmobil Res & Eng Co | regulação de genes de toxina e antitoxina para contenção biológica |
| EP2753701B1 (de) | 2011-09-27 | 2018-05-02 | ExxonMobil Research and Engineering Company | Acyl-acp-wachsestersynthasen |
| US9096834B2 (en) | 2012-02-24 | 2015-08-04 | Exxonmobil Research And Engineering Company | Recombinant microorganisms comprising thioesterase and lysophosphatidic acid acyltransferase genes for fatty acid production |
| ES2795842T3 (es) | 2012-02-24 | 2020-11-24 | Exxonmobil Res & Eng Co | Producción mejorada de ácidos grasos y derivados de ácidos grasos por microorganismos recombinantes |
| US8962299B2 (en) | 2012-02-29 | 2015-02-24 | Exxonmobil Research And Engineering Company | Four-gene pathway for wax ester synthesis |
| GB2500113A (en) | 2012-03-05 | 2013-09-11 | Oxitec Ltd | Arthropod male germline gene expression system |
| US9181568B2 (en) | 2012-04-23 | 2015-11-10 | Exxonmobil Research And Engineering Company | Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases |
| GB201303932D0 (en) | 2013-03-05 | 2013-04-17 | Oxitec Ltd | Muscle actin promoter |
| GB2526867A (en) | 2014-06-05 | 2015-12-09 | Oxitec Ltd | Gene expression system |
| KR20250058773A (ko) | 2015-09-11 | 2025-04-30 | 바이오셉터 (어스트) 피티와이 엘티디 | 키메라 항원 수용체 및 이의 용도 |
| WO2017160573A1 (en) | 2016-03-18 | 2017-09-21 | Exxonmobil Research And Engineering Company | Chlorophyllase overproduction to enhance photosynthetic efficiency |
| US20190185865A1 (en) * | 2016-07-06 | 2019-06-20 | Danmarks Tekniske Universitet | Use of prokaryotic transcriptional activators as metabolite biosensors in eukaryotic cells |
| US11866733B2 (en) | 2016-08-01 | 2024-01-09 | University of Pittsburgh—of the Commonwealth System of Higher Education | Human induced pluripotent stem cells for high efficiency genetic engineering |
| US11827876B2 (en) | 2016-08-12 | 2023-11-28 | Oxitec Ltd. | Self-limiting, sex-specific gene and methods of using |
| BR112019023477A2 (pt) | 2017-05-08 | 2020-06-30 | Gritstone Oncology, Inc. | vetores de neoantígeno de alfavírus |
| WO2019169291A1 (en) | 2018-03-02 | 2019-09-06 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Use of il-34 to treat retinal inflammation and neurodegeneration |
| AR117409A1 (es) | 2018-03-29 | 2021-08-04 | Oxitec Ltd | Noctuidas autolimitantes |
| BR112020023489A8 (pt) | 2018-05-21 | 2022-12-20 | Biosceptre Uk Ltd | Receptores de antígenos quiméricos com domínios de ligação modificados e usos dos mesmos |
| WO2020243719A1 (en) * | 2019-05-30 | 2020-12-03 | Gritstone Oncology, Inc. | Modified adenoviruses |
| US12534512B2 (en) | 2019-11-11 | 2026-01-27 | Vanderbilt University | Human monoclonal antibodies to hantavirus and methods of use therefore |
| IL300026A (en) | 2020-08-06 | 2023-03-01 | Gritstone Bio Inc | Multiepitope vaccine cassettes |
| CA3186370A1 (en) * | 2020-09-05 | 2022-03-10 | Nikos Reppas | Synthetic expression systems |
| WO2022241455A1 (en) * | 2021-05-12 | 2022-11-17 | Baylor College Of Medicine | A synthetic circuit for buffering gene dosage variation between individual mammalian cells |
| EP4355882A2 (de) * | 2021-06-15 | 2024-04-24 | Modernatx, Inc. | Manipulierte polynukleotide zur zelltyp- oder mikroumgebungsspezifischen expression |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3934454A1 (de) * | 1989-10-14 | 1991-04-18 | Merck Patent Gmbh | Regulierbare expressionsvektoren fuer bacillus |
| AU8059891A (en) * | 1990-06-13 | 1992-01-07 | Trustees Of Princeton University, The | A (lac) repressor-hsv vp16 chimeric transcriptional activator protein system functioning in transfected mammalian cells |
| GB9028062D0 (en) * | 1990-12-24 | 1991-02-13 | Agricultural & Food Res | Production of transgenic animals |
| DE4100594A1 (de) * | 1991-01-08 | 1992-07-09 | Inst Genbiologische Forschung | Neue plasmide zur zeitlichen und oertlichen kontrollierten expression eines heterologen produktes in pflanzen |
| WO1992020808A1 (en) * | 1991-05-15 | 1992-11-26 | Cell Genesys, Inc. | Genomic modifications with homologous dna targeting |
| GB9113097D0 (en) * | 1991-06-18 | 1991-08-07 | Boehringer Ingelheim Int | Chimeric gene |
| US5866755A (en) * | 1993-06-14 | 1999-02-02 | Basf Aktiengellschaft | Animals transgenic for a tetracycline-regulated transcriptional inhibitor |
| US5464758A (en) * | 1993-06-14 | 1995-11-07 | Gossen; Manfred | Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters |
-
1995
- 1995-06-07 US US08/474,169 patent/US5851796A/en not_active Expired - Lifetime
-
1996
- 1996-06-07 JP JP9502206A patent/JPH11507539A/ja not_active Ceased
- 1996-06-07 AT AT96921541T patent/ATE321871T1/de active
- 1996-06-07 WO PCT/US1996/010109 patent/WO1996040946A1/en not_active Ceased
- 1996-06-07 DE DE69635992T patent/DE69635992T2/de not_active Expired - Lifetime
- 1996-06-07 EP EP96921541A patent/EP0832254B1/de not_active Expired - Lifetime
- 1996-06-07 AU AU62745/96A patent/AU6274596A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP0832254B1 (de) | 2006-03-29 |
| DE69635992T2 (de) | 2007-02-01 |
| DE69635992D1 (de) | 2006-05-18 |
| US5851796A (en) | 1998-12-22 |
| WO1996040946A1 (en) | 1996-12-19 |
| AU6274596A (en) | 1996-12-30 |
| EP0832254A1 (de) | 1998-04-01 |
| JPH11507539A (ja) | 1999-07-06 |
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| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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