ATE321871T1 - Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten - Google Patents
Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryotenInfo
- Publication number
- ATE321871T1 ATE321871T1 AT96921541T AT96921541T ATE321871T1 AT E321871 T1 ATE321871 T1 AT E321871T1 AT 96921541 T AT96921541 T AT 96921541T AT 96921541 T AT96921541 T AT 96921541T AT E321871 T1 ATE321871 T1 AT E321871T1
- Authority
- AT
- Austria
- Prior art keywords
- tta
- tetracycline
- expression
- ttak
- autoregulatory
- Prior art date
Links
- 230000014509 gene expression Effects 0.000 title abstract 6
- 241000206602 Eukaryota Species 0.000 title 1
- 239000004098 Tetracycline Substances 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 229960002180 tetracycline Drugs 0.000 abstract 4
- 229930101283 tetracycline Natural products 0.000 abstract 4
- 235000019364 tetracycline Nutrition 0.000 abstract 4
- 150000003522 tetracyclines Chemical class 0.000 abstract 4
- 230000001042 autoregulative effect Effects 0.000 abstract 3
- 108060001084 Luciferase Proteins 0.000 abstract 2
- 239000005089 Luciferase Substances 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 1
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
- C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/474,169 US5851796A (en) | 1995-06-07 | 1995-06-07 | Autoregulatory tetracycline-regulated system for inducible gene expression in eucaryotes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE321871T1 true ATE321871T1 (de) | 2006-04-15 |
Family
ID=23882456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96921541T ATE321871T1 (de) | 1995-06-07 | 1996-06-07 | Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5851796A (de) |
| EP (1) | EP0832254B1 (de) |
| JP (1) | JPH11507539A (de) |
| AT (1) | ATE321871T1 (de) |
| AU (1) | AU6274596A (de) |
| DE (1) | DE69635992T2 (de) |
| WO (1) | WO1996040946A1 (de) |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3881382B2 (ja) * | 1995-12-01 | 2007-02-14 | イントロヘーネ ベスローテン フェンノートシャップ | 安定にトランスフェクションされた哺乳類細胞における調節されたタンパク質発現 |
| US5891718A (en) * | 1996-03-27 | 1999-04-06 | Vical Incorporated | Tetracycline inducible/repressible systems |
| DE19635568C1 (de) * | 1996-09-02 | 1998-03-26 | Gsf Forschungszentrum Umwelt | Vektorsysteme zur konditionalen Genexpression |
| WO1998037185A2 (en) * | 1997-02-20 | 1998-08-27 | The Board Of Regents Of The University Of Texas System | Vectors for controlled gene expression |
| US6087166A (en) | 1997-07-03 | 2000-07-11 | Basf Aktiengesellschaft | Transcriptional activators with graded transactivation potential |
| US6878516B1 (en) | 1998-01-16 | 2005-04-12 | Pharmacia & Upjohn Company | Autoregulatory system for validating microbial genes as possible antimicrobial targets using a tetracycline-controllable element |
| EP1049789A1 (de) * | 1998-01-16 | 2000-11-08 | PHARMACIA & UPJOHN COMPANY | Selbstreguliertes system zum testen von mikrobiellen genen, welche ein mögliches ziel von antimikrobiellen stoffen sind, unter verwendung von einem durch tetrazyklin-reguliertem element |
| US7037663B2 (en) * | 1998-02-19 | 2006-05-02 | Eastern Virginia Medical School | Human zona pellucida protein 3 and uses thereof |
| US7019114B2 (en) * | 1998-02-19 | 2006-03-28 | Eastern Virginia Medical School | Recombinant, biologically active human zona pellucida protein 3 (HZP3) to test male fertility |
| DE19831420A1 (de) * | 1998-07-14 | 2000-01-20 | Hoechst Marion Roussel De Gmbh | Expressionssysteme enthaltend chimäre Promotoren mit Bindungsstellen für rekombinante Transkriptionsfaktoren |
| US20050037335A1 (en) * | 1998-11-07 | 2005-02-17 | Wolfgang Hillen | Method for identifying novel transcriptional regulatory proteins |
| US6706491B1 (en) | 1999-04-09 | 2004-03-16 | The Board Of Trustees Of The University Of Illinois | Reagents and methods for identifying and modulating expression of genes regulated by p21 |
| ATE458818T1 (de) * | 1999-06-07 | 2010-03-15 | Tet Systems Holding Gmbh & Co | Tet repressor basierte transkriptionale regulatorische proteine |
| GB2355459B (en) | 1999-11-29 | 2001-09-26 | Isis Innovation | A dominant conditional lethal genetic system |
| JP2005516586A (ja) * | 2001-07-20 | 2005-06-09 | ボード オブ トラスティーズ オブ ザ ユニヴァースティ オブ イリノイ | 癌の処置に対する遺伝子標的を同定するための試薬および方法 |
| WO2003011118A2 (en) * | 2001-08-02 | 2003-02-13 | Trinity Biomedical Technology Corporation | Human zona pellucida proteins and methods of their use in diagnosing male infertility |
| GB2402675B (en) * | 2003-05-12 | 2008-02-20 | Oxitec Ltd | Resistance dilution |
| GB2403475B (en) * | 2003-07-01 | 2008-02-06 | Oxitec Ltd | Stable integrands |
| GB2404382B (en) | 2003-07-28 | 2008-01-30 | Oxitec Ltd | Pest control |
| US8133733B2 (en) | 2003-10-24 | 2012-03-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to target tissues |
| US8507277B2 (en) | 2003-10-24 | 2013-08-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides |
| US20090123468A1 (en) | 2003-10-24 | 2009-05-14 | Gencia Corporation | Transducible polypeptides for modifying metabolism |
| US8062891B2 (en) | 2003-10-24 | 2011-11-22 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to plants |
| AU2004297533B2 (en) | 2003-10-24 | 2010-04-29 | Gencia Corporation | Methods and compositions for delivering polynucleotides |
| JP2009502180A (ja) | 2005-08-03 | 2009-01-29 | アデレイド リサーチ アンド イノベーション ピーティーワイ リミテッド | 多糖シンターゼ |
| MX2008010285A (es) * | 2006-02-10 | 2009-01-16 | Oxitec Ltd | Sistema de expresion genetica usando un empalme alternativo en insectos. |
| GB2443186A (en) | 2006-10-25 | 2008-04-30 | Oxitec Ltd | Expression system for mediating alternative splicing |
| US8080647B2 (en) * | 2006-11-22 | 2011-12-20 | Pioneer Hi Bred International Inc | Tetracycline repressor and uses thereof |
| UA100692C2 (ru) | 2007-05-02 | 2013-01-25 | Мериал Лимитед | Днк-плазмиды, имеющие повышенную экспрессию и стабильность |
| CA2740147A1 (en) | 2008-10-28 | 2010-06-03 | Pioneer Hi-Bred International, Inc. | Sulfonylurea-responsive repressor proteins |
| AU2009324539B2 (en) | 2008-12-10 | 2015-10-01 | Synthetic Genomics, Inc. | Production of branched-chain alcohols by photosynthetic microorganisms |
| EP2448955B1 (de) | 2009-06-29 | 2015-09-30 | Synthetic Genomics, Inc. | Acyl-acp-thioesterasegene und verwendungen dafür |
| WO2011019858A1 (en) | 2009-08-11 | 2011-02-17 | Synthetic Genomics, Inc. | Microbial production of fatty alcohols |
| US20120220007A1 (en) | 2009-09-04 | 2012-08-30 | President And Fellows Of Harvard College | Production of secreted bioproducts from photosynthetic microbes |
| US9175256B2 (en) | 2010-12-23 | 2015-11-03 | Exxonmobil Research And Engineering Company | Production of fatty acids and fatty acid derivatives by recombinant microorganisms expressing polypeptides having lipolytic activity |
| EP2655615A4 (de) | 2010-12-23 | 2014-04-23 | Exxonmobil Res & Eng Co | Prokaryotische acyl-acp-thioesterasen zur herstellung von fettsäuren in genetisch modifizierten mikroorganismen |
| BR112013016020A2 (pt) | 2010-12-23 | 2017-07-11 | Exxonmobil Res & Eng Co | micro-organismos produzidos por engenharia genética compreendendo 4-hidroxibenzoil-coa tioesterases e métodos para usar os mesmos para a produção de ácidos graxos livres e derivados de ácidos graxos |
| WO2012092033A1 (en) | 2010-12-31 | 2012-07-05 | Exxonmobil Research And Engineering Company | Enhancement of biomass production by disruption of light energy dissipation pathways |
| WO2012135668A1 (en) | 2011-03-31 | 2012-10-04 | Exxonmobil Research And Engineering Company | Metabolic pathway targeting by transcription factor overexpression |
| WO2012170436A1 (en) * | 2011-06-06 | 2012-12-13 | The Regents Of The University Of California | Synthetic biology tools |
| ES2674583T3 (es) | 2011-06-30 | 2018-07-02 | Exxonmobil Research And Engineering Company | Regulación de genes de toxina y antitoxina para el confinamiento biológico |
| WO2013048557A1 (en) | 2011-09-27 | 2013-04-04 | Exxonmobil Research And Engineering Company | Acyl-acp wax ester synthases |
| ES2795842T3 (es) | 2012-02-24 | 2020-11-24 | Exxonmobil Res & Eng Co | Producción mejorada de ácidos grasos y derivados de ácidos grasos por microorganismos recombinantes |
| US9096834B2 (en) | 2012-02-24 | 2015-08-04 | Exxonmobil Research And Engineering Company | Recombinant microorganisms comprising thioesterase and lysophosphatidic acid acyltransferase genes for fatty acid production |
| US8962299B2 (en) | 2012-02-29 | 2015-02-24 | Exxonmobil Research And Engineering Company | Four-gene pathway for wax ester synthesis |
| GB2500113A (en) | 2012-03-05 | 2013-09-11 | Oxitec Ltd | Arthropod male germline gene expression system |
| US9181568B2 (en) | 2012-04-23 | 2015-11-10 | Exxonmobil Research And Engineering Company | Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases |
| GB201303932D0 (en) | 2013-03-05 | 2013-04-17 | Oxitec Ltd | Muscle actin promoter |
| GB2526867A (en) | 2014-06-05 | 2015-12-09 | Oxitec Ltd | Gene expression system |
| US12121539B2 (en) | 2015-09-11 | 2024-10-22 | Biosceptre (Aust) Pty Ltd | Chimeric antigen receptors and uses thereof |
| EP3430123A1 (de) | 2016-03-18 | 2019-01-23 | ExxonMobil Research and Engineering Company | Chlorophyllaseüberproduktion zur erhöhung der photosynthetischen effizienz |
| US20190185865A1 (en) * | 2016-07-06 | 2019-06-20 | Danmarks Tekniske Universitet | Use of prokaryotic transcriptional activators as metabolite biosensors in eukaryotic cells |
| EP3491134B1 (de) | 2016-08-01 | 2023-10-11 | University of Pittsburgh - of The Commonwealth System of Higher Education | Menschliche induzierte pluripotente stammzellen zur hocheffizienten genmanipulation |
| BR112019002771A2 (pt) | 2016-08-12 | 2019-05-14 | Oxitec Ltd | polinucleotídeo de módulo de controle de união de doublesex, sistema de expressão de gene, plasmídeo vetor de expressão, inseto geneticamente engenheirado, métodos para produzir insetos geneticamente engenheirados, para cultivar seletivamente insetos macho geneticamente engenheirados, para reduzir uma população de inseto selvagem, para criar um mosquito aedes aegypti transgênico e para detectar a presença de uma molécula de dna, local alvo cromossômico de aedes aegypti, mosquito aedes aegypti geneticamente engenheirado, molécula de dna, e, kit de detecção de dna. |
| KR102945203B1 (ko) | 2017-05-08 | 2026-03-31 | 시애틀 프로젝트 코포레이션 | 알파바이러스 신생항원 벡터 |
| EP3758736B1 (de) | 2018-03-02 | 2024-05-01 | The United States of America, as represented by The Secretary, Department of Health and Human Services | Il-34 zur anwendung in einem verfahren zur behandlung von retinaler entzündung und neurodegeneration |
| AR117409A1 (es) | 2018-03-29 | 2021-08-04 | Oxitec Ltd | Noctuidas autolimitantes |
| AU2019275479B2 (en) | 2018-05-21 | 2022-12-08 | Biosceptre (Aust) Pty Ltd | Chimeric antigen receptors with modified linker domains and uses thereof |
| KR20210090650A (ko) | 2018-11-07 | 2021-07-20 | 그릿스톤 바이오, 인코포레이티드 | 알파바이러스 신생항원 벡터 및 인터페론 억제제 |
| SG11202113187WA (en) | 2019-05-30 | 2021-12-30 | Gritstone Bio Inc | Modified adenoviruses |
| WO2021096829A1 (en) * | 2019-11-11 | 2021-05-20 | Vanderbilt University | Human monoclonal antibodies to hantavirus and methods of use therefor |
| IL300026A (en) | 2020-08-06 | 2023-03-01 | Gritstone Bio Inc | Multiepitope vaccine cassettes |
| EP4208555A4 (de) * | 2020-09-05 | 2025-01-08 | Ginkgo Bioworks, Inc. | Synthetische expressionssysteme |
| WO2022241455A1 (en) * | 2021-05-12 | 2022-11-17 | Baylor College Of Medicine | A synthetic circuit for buffering gene dosage variation between individual mammalian cells |
| US20240384277A1 (en) * | 2021-06-15 | 2024-11-21 | Modernatx, Inc. | Engineered polynucleotides for cell-type or microenvironment-specific expression |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3934454A1 (de) * | 1989-10-14 | 1991-04-18 | Merck Patent Gmbh | Regulierbare expressionsvektoren fuer bacillus |
| AU8059891A (en) * | 1990-06-13 | 1992-01-07 | Trustees Of Princeton University, The | A (lac) repressor-hsv vp16 chimeric transcriptional activator protein system functioning in transfected mammalian cells |
| GB9028062D0 (en) * | 1990-12-24 | 1991-02-13 | Agricultural & Food Res | Production of transgenic animals |
| DE4100594A1 (de) * | 1991-01-08 | 1992-07-09 | Inst Genbiologische Forschung | Neue plasmide zur zeitlichen und oertlichen kontrollierten expression eines heterologen produktes in pflanzen |
| AU664847B2 (en) * | 1991-05-15 | 1995-12-07 | Cell Genesys, Inc. | Genomic modifications with homologous DNA targeting |
| GB9113097D0 (en) * | 1991-06-18 | 1991-08-07 | Boehringer Ingelheim Int | Chimeric gene |
| US5464758A (en) * | 1993-06-14 | 1995-11-07 | Gossen; Manfred | Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters |
| US5866755A (en) * | 1993-06-14 | 1999-02-02 | Basf Aktiengellschaft | Animals transgenic for a tetracycline-regulated transcriptional inhibitor |
-
1995
- 1995-06-07 US US08/474,169 patent/US5851796A/en not_active Expired - Lifetime
-
1996
- 1996-06-07 JP JP9502206A patent/JPH11507539A/ja not_active Ceased
- 1996-06-07 EP EP96921541A patent/EP0832254B1/de not_active Expired - Lifetime
- 1996-06-07 AU AU62745/96A patent/AU6274596A/en not_active Abandoned
- 1996-06-07 AT AT96921541T patent/ATE321871T1/de active
- 1996-06-07 DE DE69635992T patent/DE69635992T2/de not_active Expired - Lifetime
- 1996-06-07 WO PCT/US1996/010109 patent/WO1996040946A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| DE69635992T2 (de) | 2007-02-01 |
| EP0832254B1 (de) | 2006-03-29 |
| DE69635992D1 (de) | 2006-05-18 |
| AU6274596A (en) | 1996-12-30 |
| JPH11507539A (ja) | 1999-07-06 |
| EP0832254A1 (de) | 1998-04-01 |
| US5851796A (en) | 1998-12-22 |
| WO1996040946A1 (en) | 1996-12-19 |
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| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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