ATE321871T1 - Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten - Google Patents
Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryotenInfo
- Publication number
- ATE321871T1 ATE321871T1 AT96921541T AT96921541T ATE321871T1 AT E321871 T1 ATE321871 T1 AT E321871T1 AT 96921541 T AT96921541 T AT 96921541T AT 96921541 T AT96921541 T AT 96921541T AT E321871 T1 ATE321871 T1 AT E321871T1
- Authority
- AT
- Austria
- Prior art keywords
- tta
- tetracycline
- expression
- ttak
- autoregulatory
- Prior art date
Links
- 230000014509 gene expression Effects 0.000 title abstract 6
- 241000206602 Eukaryota Species 0.000 title 1
- 239000004098 Tetracycline Substances 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 229960002180 tetracycline Drugs 0.000 abstract 4
- 229930101283 tetracycline Natural products 0.000 abstract 4
- 235000019364 tetracycline Nutrition 0.000 abstract 4
- 150000003522 tetracyclines Chemical class 0.000 abstract 4
- 230000001042 autoregulative effect Effects 0.000 abstract 3
- 108060001084 Luciferase Proteins 0.000 abstract 2
- 239000005089 Luciferase Substances 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 1
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
- C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/474,169 US5851796A (en) | 1995-06-07 | 1995-06-07 | Autoregulatory tetracycline-regulated system for inducible gene expression in eucaryotes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE321871T1 true ATE321871T1 (de) | 2006-04-15 |
Family
ID=23882456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96921541T ATE321871T1 (de) | 1995-06-07 | 1996-06-07 | Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5851796A (de) |
| EP (1) | EP0832254B1 (de) |
| JP (1) | JPH11507539A (de) |
| AT (1) | ATE321871T1 (de) |
| AU (1) | AU6274596A (de) |
| DE (1) | DE69635992T2 (de) |
| WO (1) | WO1996040946A1 (de) |
Families Citing this family (64)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0870044T3 (da) * | 1995-12-01 | 2004-08-02 | Crucell Holland Bv | Reguleret proteinekspression i stabilt transfekterede pattedyrsceller |
| US5891718A (en) * | 1996-03-27 | 1999-04-06 | Vical Incorporated | Tetracycline inducible/repressible systems |
| DE19635568C1 (de) * | 1996-09-02 | 1998-03-26 | Gsf Forschungszentrum Umwelt | Vektorsysteme zur konditionalen Genexpression |
| WO1998037185A2 (en) * | 1997-02-20 | 1998-08-27 | The Board Of Regents Of The University Of Texas System | Vectors for controlled gene expression |
| US6087166A (en) * | 1997-07-03 | 2000-07-11 | Basf Aktiengesellschaft | Transcriptional activators with graded transactivation potential |
| US6878516B1 (en) | 1998-01-16 | 2005-04-12 | Pharmacia & Upjohn Company | Autoregulatory system for validating microbial genes as possible antimicrobial targets using a tetracycline-controllable element |
| AU2312699A (en) * | 1998-01-16 | 1999-08-02 | Pharmacia & Upjohn Company | An autoregulatory system for validating microbial genes as possible antimicrobial targets using tetracycline-controllable element |
| BR9908096A (pt) * | 1998-02-19 | 2000-10-31 | Eastern Virginia Med School | Proteìna 3 da zona pelúcida humana (hzp3), ativa, recombinante |
| US7037663B2 (en) * | 1998-02-19 | 2006-05-02 | Eastern Virginia Medical School | Human zona pellucida protein 3 and uses thereof |
| DE19831420A1 (de) * | 1998-07-14 | 2000-01-20 | Hoechst Marion Roussel De Gmbh | Expressionssysteme enthaltend chimäre Promotoren mit Bindungsstellen für rekombinante Transkriptionsfaktoren |
| US20050037335A1 (en) * | 1998-11-07 | 2005-02-17 | Wolfgang Hillen | Method for identifying novel transcriptional regulatory proteins |
| US6706491B1 (en) | 1999-04-09 | 2004-03-16 | The Board Of Trustees Of The University Of Illinois | Reagents and methods for identifying and modulating expression of genes regulated by p21 |
| ATE458818T1 (de) * | 1999-06-07 | 2010-03-15 | Tet Systems Holding Gmbh & Co | Tet repressor basierte transkriptionale regulatorische proteine |
| GB2355459B (en) | 1999-11-29 | 2001-09-26 | Isis Innovation | A dominant conditional lethal genetic system |
| JP2005516586A (ja) * | 2001-07-20 | 2005-06-09 | ボード オブ トラスティーズ オブ ザ ユニヴァースティ オブ イリノイ | 癌の処置に対する遺伝子標的を同定するための試薬および方法 |
| EP1421104A4 (de) * | 2001-08-02 | 2005-08-24 | Trinity Biomedical Technology | Humane zona-pellucida-proteine und verfahren zu deren verwendung bei der diagnose männlicher unfruchtbarkeit |
| GB2402675B (en) * | 2003-05-12 | 2008-02-20 | Oxitec Ltd | Resistance dilution |
| GB2403475B (en) * | 2003-07-01 | 2008-02-06 | Oxitec Ltd | Stable integrands |
| GB2404382B (en) | 2003-07-28 | 2008-01-30 | Oxitec Ltd | Pest control |
| US20090123468A1 (en) | 2003-10-24 | 2009-05-14 | Gencia Corporation | Transducible polypeptides for modifying metabolism |
| US8507277B2 (en) | 2003-10-24 | 2013-08-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides |
| WO2005056752A2 (en) | 2003-10-24 | 2005-06-23 | Gencia Corporation | Methods and compositions for delivering polynucleotides |
| US8133733B2 (en) | 2003-10-24 | 2012-03-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to target tissues |
| US8062891B2 (en) | 2003-10-24 | 2011-11-22 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to plants |
| US20080307549A1 (en) | 2005-08-03 | 2008-12-11 | Adelaide Research & Innovation Pty Ltd. | Polysaccharide Synthases |
| GB2443186A (en) | 2006-10-25 | 2008-04-30 | Oxitec Ltd | Expression system for mediating alternative splicing |
| EP3159414A1 (de) * | 2006-02-10 | 2017-04-26 | Oxitec Limited | Expressionssystem |
| US8080647B2 (en) * | 2006-11-22 | 2011-12-20 | Pioneer Hi Bred International Inc | Tetracycline repressor and uses thereof |
| AU2007352618B2 (en) | 2007-05-02 | 2013-07-18 | Boehringer Ingelheim Animal Health USA Inc. | DNA plasmids having improved expression and stability |
| EP2364322A1 (de) * | 2008-10-28 | 2011-09-14 | Pioneer Hi-Bred International Inc. | Sulfonylharnstoffreaktive repressorproteine |
| US8124400B2 (en) | 2008-12-10 | 2012-02-28 | Synthetic Genomics, Inc. | Production of branched-chain alcohols by photosynthetic microorganisms |
| WO2011008565A1 (en) | 2009-06-29 | 2011-01-20 | Synthetic Genomics, Inc. | Acyl-acp thioesterase genes and uses therefor |
| WO2011019858A1 (en) | 2009-08-11 | 2011-02-17 | Synthetic Genomics, Inc. | Microbial production of fatty alcohols |
| EP2719757A1 (de) | 2009-09-04 | 2014-04-16 | President and Fellows of Harvard College | Herstellung sekretierter bioprodukte aus photosynthese-mikroben |
| EP2655615A4 (de) | 2010-12-23 | 2014-04-23 | Exxonmobil Res & Eng Co | Prokaryotische acyl-acp-thioesterasen zur herstellung von fettsäuren in genetisch modifizierten mikroorganismen |
| AU2011349729B2 (en) | 2010-12-23 | 2015-09-24 | Exxonmobil Research And Engineering Company | Genetically engineered microorganisms comprising 4-hydroxybenzoly-CoA thioesterases and methods of using the same for producing free fatty acids and fatty acid derivatives |
| US9187726B2 (en) | 2010-12-23 | 2015-11-17 | Exxonmobil Research And Engineering Company | Culturing a microorganism in a medium with an elevated level of a carboxylate counterion source |
| EP2658963A4 (de) | 2010-12-31 | 2014-05-14 | Exxonmobil Res & Eng Co | Verbesserung der biomasseproduktion durch disruption von lichtenergiedissipationswegen |
| WO2012135668A1 (en) | 2011-03-31 | 2012-10-04 | Exxonmobil Research And Engineering Company | Metabolic pathway targeting by transcription factor overexpression |
| US20130005590A1 (en) | 2011-06-06 | 2013-01-03 | The Regents Of The University Of California | Synthetic biology tools |
| MX2013015327A (es) | 2011-06-30 | 2014-01-31 | Exxonmobil Res & Eng Co | Regulacion de genes toxina y antitoxina para contencion biologica. |
| CN103975070A (zh) | 2011-09-27 | 2014-08-06 | 艾克森美孚研究与工程公司 | 酰基-酰基载体蛋白(acyl-acp)蜡酯合酶 |
| US9096834B2 (en) | 2012-02-24 | 2015-08-04 | Exxonmobil Research And Engineering Company | Recombinant microorganisms comprising thioesterase and lysophosphatidic acid acyltransferase genes for fatty acid production |
| PT2814972T (pt) | 2012-02-24 | 2020-06-16 | Exxonmobil Res & Eng Co | Produção melhorada de ácidos gordos e derivados de ácidos gordos por micro-organismos recombinantes |
| US8962299B2 (en) | 2012-02-29 | 2015-02-24 | Exxonmobil Research And Engineering Company | Four-gene pathway for wax ester synthesis |
| GB2500113A (en) | 2012-03-05 | 2013-09-11 | Oxitec Ltd | Arthropod male germline gene expression system |
| US9181568B2 (en) | 2012-04-23 | 2015-11-10 | Exxonmobil Research And Engineering Company | Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases |
| GB201303932D0 (en) | 2013-03-05 | 2013-04-17 | Oxitec Ltd | Muscle actin promoter |
| GB2526867A (en) | 2014-06-05 | 2015-12-09 | Oxitec Ltd | Gene expression system |
| AU2016318230B2 (en) | 2015-09-11 | 2020-05-21 | Biosceptre (Aust) Pty Ltd | Chimeric antigen receptors and uses thereof |
| US20170267984A1 (en) | 2016-03-18 | 2017-09-21 | Exxonmobil Research And Engineering Company | Chlorophyllase overproduction to enhance photosynthetic efficiency |
| WO2018007526A1 (en) * | 2016-07-06 | 2018-01-11 | Danmarks Tekniske Universitet | Use of prokaryotic transcriptional activators as metabolite biosensors in eukaryotic cells |
| US11866733B2 (en) | 2016-08-01 | 2024-01-09 | University of Pittsburgh—of the Commonwealth System of Higher Education | Human induced pluripotent stem cells for high efficiency genetic engineering |
| US11827876B2 (en) | 2016-08-12 | 2023-11-28 | Oxitec Ltd. | Self-limiting, sex-specific gene and methods of using |
| TW201907937A (zh) | 2017-05-08 | 2019-03-01 | 美商葛利史東腫瘤科技公司 | 阿爾法病毒新抗原載體 |
| WO2019169291A1 (en) | 2018-03-02 | 2019-09-06 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Use of il-34 to treat retinal inflammation and neurodegeneration |
| AR117409A1 (es) | 2018-03-29 | 2021-08-04 | Oxitec Ltd | Noctuidas autolimitantes |
| CN112166193A (zh) | 2018-05-21 | 2021-01-01 | 生物权威（英国）有限公司 | 具有经修饰的接头结构域的嵌合抗原受体及其用途 |
| MX2021014525A (es) | 2019-05-30 | 2022-03-17 | Gritstone Bio Inc | Adenovirus modificados. |
| US12534512B2 (en) | 2019-11-11 | 2026-01-27 | Vanderbilt University | Human monoclonal antibodies to hantavirus and methods of use therefore |
| EP4192496A4 (de) | 2020-08-06 | 2025-01-01 | Gritstone bio, Inc. | Multiepitop-impfstoffkassetten |
| AU2021337747A1 (en) * | 2020-09-05 | 2023-02-23 | Ginkgo Bioworks, Inc. | Synthetic expression systems |
| WO2022241455A1 (en) * | 2021-05-12 | 2022-11-17 | Baylor College Of Medicine | A synthetic circuit for buffering gene dosage variation between individual mammalian cells |
| US20240384277A1 (en) * | 2021-06-15 | 2024-11-21 | Modernatx, Inc. | Engineered polynucleotides for cell-type or microenvironment-specific expression |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3934454A1 (de) * | 1989-10-14 | 1991-04-18 | Merck Patent Gmbh | Regulierbare expressionsvektoren fuer bacillus |
| WO1991019784A1 (en) * | 1990-06-13 | 1991-12-26 | The Trustees Of Princeton University | A Lac REPRESSOR-HSV VP16 CHIMERIC TRANSCRIPTIONAL ACTIVATOR PROTEIN SYSTEM FUNCTIONING IN TRANSFECTED MAMMALIAN CELLS |
| GB9028062D0 (en) * | 1990-12-24 | 1991-02-13 | Agricultural & Food Res | Production of transgenic animals |
| DE4100594A1 (de) * | 1991-01-08 | 1992-07-09 | Inst Genbiologische Forschung | Neue plasmide zur zeitlichen und oertlichen kontrollierten expression eines heterologen produktes in pflanzen |
| AU664847B2 (en) * | 1991-05-15 | 1995-12-07 | Cell Genesys, Inc. | Genomic modifications with homologous DNA targeting |
| GB9113097D0 (en) * | 1991-06-18 | 1991-08-07 | Boehringer Ingelheim Int | Chimeric gene |
| US5464758A (en) * | 1993-06-14 | 1995-11-07 | Gossen; Manfred | Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters |
| US5866755A (en) * | 1993-06-14 | 1999-02-02 | Basf Aktiengellschaft | Animals transgenic for a tetracycline-regulated transcriptional inhibitor |
-
1995
- 1995-06-07 US US08/474,169 patent/US5851796A/en not_active Expired - Lifetime
-
1996
- 1996-06-07 EP EP96921541A patent/EP0832254B1/de not_active Expired - Lifetime
- 1996-06-07 WO PCT/US1996/010109 patent/WO1996040946A1/en not_active Ceased
- 1996-06-07 AT AT96921541T patent/ATE321871T1/de active
- 1996-06-07 JP JP9502206A patent/JPH11507539A/ja not_active Ceased
- 1996-06-07 DE DE69635992T patent/DE69635992T2/de not_active Expired - Lifetime
- 1996-06-07 AU AU62745/96A patent/AU6274596A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE69635992T2 (de) | 2007-02-01 |
| WO1996040946A1 (en) | 1996-12-19 |
| EP0832254A1 (de) | 1998-04-01 |
| EP0832254B1 (de) | 2006-03-29 |
| US5851796A (en) | 1998-12-22 |
| AU6274596A (en) | 1996-12-30 |
| JPH11507539A (ja) | 1999-07-06 |
| DE69635992D1 (de) | 2006-05-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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