ATE330008T1 - Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen - Google Patents
Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisenInfo
- Publication number
- ATE330008T1 ATE330008T1 AT01983959T AT01983959T ATE330008T1 AT E330008 T1 ATE330008 T1 AT E330008T1 AT 01983959 T AT01983959 T AT 01983959T AT 01983959 T AT01983959 T AT 01983959T AT E330008 T1 ATE330008 T1 AT E330008T1
- Authority
- AT
- Austria
- Prior art keywords
- banks
- forming oligonucleotide
- libraries
- mutation distribution
- controlled mutation
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/699,250 US6582914B1 (en) | 2000-10-26 | 2000-10-26 | Method for generating a library of oligonucleotides comprising a controlled distribution of mutations |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE330008T1 true ATE330008T1 (de) | 2006-07-15 |
Family
ID=24808514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01983959T ATE330008T1 (de) | 2000-10-26 | 2001-10-12 | Methode zur bildung von oligonucleotidbanken welche eine kontrollierte mutationsverteilung aufweisen |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6582914B1 (de) |
| EP (1) | EP1328627B1 (de) |
| AT (1) | ATE330008T1 (de) |
| AU (1) | AU2002215346A1 (de) |
| CA (1) | CA2426774A1 (de) |
| DE (1) | DE60120733T2 (de) |
| DK (1) | DK1328627T3 (de) |
| WO (1) | WO2002034762A2 (de) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5340742A (en) * | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids |
| US6893877B2 (en) | 1998-01-12 | 2005-05-17 | Massachusetts Institute Of Technology | Methods for screening substances in a microwell array |
| NZ513390A (en) | 1999-03-19 | 2003-01-31 | Genencor Int | Multi-through hole testing plate for high throughput screening |
| US20020151040A1 (en) | 2000-02-18 | 2002-10-17 | Matthew O' Keefe | Apparatus and methods for parallel processing of microvolume liquid reactions |
| US20020155439A1 (en) * | 2000-12-04 | 2002-10-24 | Ana Rodriguez | Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction |
| JP4210769B2 (ja) * | 2001-11-22 | 2009-01-21 | 独立行政法人産業技術総合研究所 | Ｇａｔｅｗａｙエントリークローンの作製方法 |
| US8277753B2 (en) | 2002-08-23 | 2012-10-02 | Life Technologies Corporation | Microfluidic transfer pin |
| WO2004074818A2 (en) | 2002-12-20 | 2004-09-02 | Biotrove, Inc. | Assay apparatus and method using microfluidic arrays |
| US20050084868A1 (en) * | 2003-10-16 | 2005-04-21 | Wolfgang Aehle | Generation of stabilized proteins by combinatorial consensus mutagenesis |
| US7985569B2 (en) | 2003-11-19 | 2011-07-26 | Danisco Us Inc. | Cellulomonas 69B4 serine protease variants |
| CA2559171A1 (en) | 2004-03-12 | 2005-09-29 | Biotrove, Inc. | Nanoliter array loading |
| US20050233336A1 (en) * | 2004-04-19 | 2005-10-20 | O'maille Paul E | Compositions and methods for producing libraries with controlled compositions and screening probabilities |
| EP1915446B1 (de) | 2005-08-11 | 2017-06-14 | Synthetic Genomics, Inc. | In vitro-rekombinationsmethode |
| GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
| PT2032698E (pt) * | 2006-06-23 | 2012-09-24 | Danisco Us Inc | Avaliação sistemática de relações entre sequência e actividade utilizando bibliotecas de avaliação de locais em relação a múltiplas propriedades de engenharia |
| MX2009003472A (es) * | 2006-10-10 | 2009-06-02 | Danisco Us Inc Genencor Div | Variantes de glucoamilasa con propiedades alteradas. |
| BRPI0812448A2 (pt) * | 2007-06-06 | 2014-10-14 | Danisco Us Inc Genecor Division | Método para aperfeiçoamento de propriedades de proteína |
| PL2481796T3 (pl) | 2007-10-09 | 2014-11-28 | Danisco Us Inc | Odmiany glukoamylazy |
| US8592194B2 (en) | 2007-10-09 | 2013-11-26 | Danisco Us Inc. | Glucoamylase variants with altered properties |
| EP2205732A2 (de) | 2007-11-01 | 2010-07-14 | Danisco US Inc. | Herstellung von thermolysin und varianten davon sowie deren verwendung in flüssigreinigungsmitteln |
| DK3323889T3 (da) | 2007-11-20 | 2020-06-29 | Danisco Us Inc | Glucoamylasevarianter med ændrede egenskaber |
| DK2235190T3 (en) | 2007-12-13 | 2018-07-23 | Danisco Us Inc | COMPOSITIONS AND PROCEDURES FOR PRODUCING ISOPRENE |
| EP2255013B1 (de) * | 2008-02-15 | 2016-06-08 | Synthetic Genomics, Inc. | Verfahren zur in-vitro-zusammenfügung und kombinatorischen konstruktion von nukleinsäuremolekülen |
| JP2011518564A (ja) | 2008-04-23 | 2011-06-30 | ダニスコ・ユーエス・インク | 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体 |
| EP2130918A1 (de) * | 2008-06-05 | 2009-12-09 | C-Lecta GmbH | Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen |
| WO2009149144A2 (en) | 2008-06-06 | 2009-12-10 | Danisco Us Inc. | Compositions and methods comprising variant microbial proteases |
| US20090312196A1 (en) * | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| US8383346B2 (en) * | 2008-06-13 | 2013-02-26 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
| US20100192985A1 (en) * | 2008-11-11 | 2010-08-05 | Wolfgang Aehle | Compositions and methods comprising serine protease variants |
| EP2362896A2 (de) | 2008-11-11 | 2011-09-07 | Danisco US Inc. | Bacillus subtilisin mit mindestens einer kombinierbaren mutation |
| US8518686B2 (en) | 2009-04-23 | 2013-08-27 | Danisco Us Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
| JP5827227B2 (ja) | 2009-08-19 | 2015-12-02 | デュポン ニュートリション バイオサイエンシーズ エーピーエスＤｕＰｏｎｔ Ｎｕｔｒｉｔｉｏｎ Ｂｉｏｓｃｉｅｎｃｅｓ ＡｐＳ | グルコアミラーゼの変異体 |
| WO2011022465A1 (en) | 2009-08-19 | 2011-02-24 | Danisco Us Inc. | Combinatorial variants of glucoamylase with improved specific activity and/or thermostability |
| JP5863666B2 (ja) | 2009-12-09 | 2016-02-17 | ダニスコ・ユーエス・インク | プロテアーゼ変異体を含む組成物及び方法 |
| EP2558573B1 (de) | 2010-04-15 | 2017-02-22 | Danisco US Inc. | Zusammensetzungen und verfahren mit proteasevarianten |
| PL3575389T3 (pl) | 2010-05-06 | 2025-06-02 | The Procter & Gamble Company | Produkty konsumenckie z odmianami proteaz |
| CN103443271A (zh) | 2010-10-27 | 2013-12-11 | 丹尼斯科美国公司 | 用于增加异戊二烯产量的异戊二烯合酶变体 |
| MX378782B (es) | 2011-05-05 | 2025-03-10 | Danisco Us Inc | Composiciones y metodos que comprenden variantes de proteasa serina. |
| MX357386B (es) | 2011-05-05 | 2018-07-06 | Procter & Gamble | Composiciones y metodos que comprenden variantes de proteasa serina. |
| US9677058B2 (en) | 2011-12-22 | 2017-06-13 | Dupont Nutrition Biosciences Aps | Polypeptides having glucoamylase activity and method of producing the same |
| US9163263B2 (en) | 2012-05-02 | 2015-10-20 | The Goodyear Tire & Rubber Company | Identification of isoprene synthase variants with improved properties for the production of isoprene |
| MX2015002099A (es) | 2012-08-22 | 2015-05-11 | Dupont Nutrition Biosci Aps | Variantes que tienen actividad glucoamilasa. |
| AU2013337255A1 (en) | 2012-11-05 | 2015-04-02 | Danisco Us Inc. | Compositions and methods comprising thermolysin protease variants |
| US20140288097A1 (en) | 2013-02-07 | 2014-09-25 | The Regents Of The University Of California | Use of translational profiling to identify target molecules for therapeutic treatment |
| US20160222368A1 (en) | 2013-09-12 | 2016-08-04 | Danisco Us Inc. | Compositions and Methods Comprising LG12-CLADE Protease Variants |
| JP6594955B2 (ja) | 2014-08-27 | 2019-10-23 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | シントン形成 |
| US9963687B2 (en) | 2014-08-27 | 2018-05-08 | New England Biolabs, Inc. | Fusion polymerase and method for using the same |
| EP3872174B1 (de) | 2015-05-13 | 2023-03-01 | Danisco US Inc. | Aprl-clade-protease-varianten and verwendungen davon |
| MX2022013462A (es) | 2020-04-27 | 2022-11-16 | 4D Molecular Therapeutics Inc | Variantes, formulaciones y metodos adeno-asociados para suministro pulmonar. |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE93541T1 (de) | 1985-07-03 | 1993-09-15 | Genencor Int | Hybride polypeptide und verfahren zu deren herstellung. |
| EP0241487B1 (de) | 1985-08-28 | 1998-04-22 | PIECZENIK, George | Verfahren und mittel zur sortierung und bestimmung biologischer informationen |
| US5556747A (en) | 1990-07-09 | 1996-09-17 | E. R. Squibb & Sons, Inc. | Method for site-directed mutagenesis |
| WO1992002536A1 (en) * | 1990-08-02 | 1992-02-20 | The Regents Of The University Of Colorado | Systematic polypeptide evolution by reverse translation |
| GB9027836D0 (en) | 1990-12-21 | 1991-02-13 | Unilever Plc | Enzymes and enzymatic detergent compositions |
| US5270170A (en) | 1991-10-16 | 1993-12-14 | Affymax Technologies N.V. | Peptide library and screening method |
| US5521077A (en) | 1994-04-28 | 1996-05-28 | The Leland Stanford Junior University | Method of generating multiple protein variants and populations of protein variants prepared thereby |
| DK0843725T3 (da) | 1995-08-11 | 2002-08-12 | Novozymes As | Fremgangmåde til fremstilling af polypeptidvarianter |
| GB9521788D0 (en) | 1995-10-24 | 1996-01-03 | Univ Leeds | DNA repair |
| US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| US6242222B1 (en) | 1996-06-07 | 2001-06-05 | Massachusetts Institute Of Technology | Programmed sequential mutagenesis |
| JPH1066576A (ja) | 1996-08-07 | 1998-03-10 | Novo Nordisk As | 突出末端を有する２本鎖ｄｎａ及びこれを用いたｄｎａのシャフリング方法 |
| FR2753202A1 (fr) | 1996-09-06 | 1998-03-13 | Agronomique Inst Nat Rech | Bacteries multiresistantes, procedes d'obtention et utilisation |
| WO1998017684A2 (en) | 1996-10-25 | 1998-04-30 | Advanced Research & Technology Institute | Dna sequences encoding fusions of dna repair proteins and uses thereof |
| EP0948615B1 (de) | 1996-12-20 | 2004-12-15 | Novozymes A/S | In vivo rekombination |
| BR9808368A (pt) | 1997-03-18 | 2000-05-23 | Novo Nordisk As | Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse. |
| DK0996718T3 (da) | 1997-03-18 | 2009-04-06 | Novozymes As | Fremgangsmåde til konstruktion af et bibliotek ved anvendelse af DNA-shuffling |
| NZ337910A (en) | 1997-03-18 | 2001-10-26 | Novo Nordisk As | Shuffling of heterologous DNA sequences using conserved regions |
| US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| AU7380898A (en) | 1997-05-12 | 1998-12-08 | Thermogen, Inc. | Method for the stabilization of proteins and the thermostabilized alcohol dehydrogenases produced thereby |
| US6251604B1 (en) * | 1999-08-13 | 2001-06-26 | Genopsys, Inc. | Random mutagenesis and amplification of nucleic acid |
-
2000
- 2000-10-26 US US09/699,250 patent/US6582914B1/en not_active Expired - Lifetime
-
2001
- 2001-10-12 CA CA002426774A patent/CA2426774A1/en not_active Abandoned
- 2001-10-12 DK DK01983959T patent/DK1328627T3/da active
- 2001-10-12 WO PCT/US2001/032046 patent/WO2002034762A2/en not_active Ceased
- 2001-10-12 EP EP01983959A patent/EP1328627B1/de not_active Expired - Lifetime
- 2001-10-12 AT AT01983959T patent/ATE330008T1/de not_active IP Right Cessation
- 2001-10-12 AU AU2002215346A patent/AU2002215346A1/en not_active Abandoned
- 2001-10-12 DE DE60120733T patent/DE60120733T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1328627A2 (de) | 2003-07-23 |
| US6582914B1 (en) | 2003-06-24 |
| DE60120733T2 (de) | 2007-06-14 |
| WO2002034762A2 (en) | 2002-05-02 |
| WO2002034762A3 (en) | 2003-03-13 |
| EP1328627B1 (de) | 2006-06-14 |
| DK1328627T3 (da) | 2006-10-23 |
| CA2426774A1 (en) | 2002-05-02 |
| AU2002215346A1 (en) | 2002-05-06 |
| DE60120733D1 (de) | 2006-07-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |