ATE118818T1 - Verfahren zur herstellung synthetischer oligonukleotide, die spezifisch an bestimmungsstellen auf duplex-dna-molekülen binden durch formen eines kolinearen triplex, die synthetischen oligonukleotide und verfahren zu ihrer herstellung. - Google Patents

Verfahren zur herstellung synthetischer oligonukleotide, die spezifisch an bestimmungsstellen auf duplex-dna-molekülen binden durch formen eines kolinearen triplex, die synthetischen oligonukleotide und verfahren zu ihrer herstellung.

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Publication number
ATE118818T1
ATE118818T1 AT89313391T AT89313391T ATE118818T1 AT E118818 T1 ATE118818 T1 AT E118818T1 AT 89313391 T AT89313391 T AT 89313391T AT 89313391 T AT89313391 T AT 89313391T AT E118818 T1 ATE118818 T1 AT E118818T1
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Austria
Prior art keywords
synthetic oligonucleotides
dna
bind
duplex
duplex dna
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AT89313391T
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English (en)
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Michael Edward Hogan
Donald Joseph Kessler
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Baylor College Medicine
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Publication of ATE118818T1 publication Critical patent/ATE118818T1/de

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    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
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AT89313391T 1988-12-20 1989-12-20 Verfahren zur herstellung synthetischer oligonukleotide, die spezifisch an bestimmungsstellen auf duplex-dna-molekülen binden durch formen eines kolinearen triplex, die synthetischen oligonukleotide und verfahren zu ihrer herstellung. ATE118818T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US28735988A 1988-12-20 1988-12-20

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Publication Number Publication Date
ATE118818T1 true ATE118818T1 (de) 1995-03-15

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AT89313391T ATE118818T1 (de) 1988-12-20 1989-12-20 Verfahren zur herstellung synthetischer oligonukleotide, die spezifisch an bestimmungsstellen auf duplex-dna-molekülen binden durch formen eines kolinearen triplex, die synthetischen oligonukleotide und verfahren zu ihrer herstellung.

Country Status (10)

Country Link
EP (2) EP0449972A4 (de)
JP (1) JPH04502407A (de)
AT (1) ATE118818T1 (de)
AU (1) AU640910B2 (de)
CA (1) CA2006008C (de)
DE (1) DE68921315T2 (de)
DK (1) DK120091A (de)
ES (1) ES2069598T3 (de)
PT (1) PT92640B (de)
WO (1) WO1990006934A1 (de)

Families Citing this family (120)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928863A (en) * 1986-11-26 1999-07-27 Princeton University Triple-stranded nucleic acids
US5422251A (en) * 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
US5849482A (en) * 1988-09-28 1998-12-15 Epoch Pharmaceuticals, Inc. Crosslinking oligonucleotides
US5721218A (en) * 1989-10-23 1998-02-24 Gilead Sciences, Inc. Oligonucleotides with inverted polarity
US5399676A (en) * 1989-10-23 1995-03-21 Gilead Sciences Oligonucleotides with inverted polarity
IE20010427A1 (en) * 1990-04-10 2003-03-05 Canji Inc Gene therapy for cell proliferative diseases
WO1991018997A1 (en) * 1990-05-25 1991-12-12 Gilead Sciences, Inc. Sequence-specific nonphotoactivated crosslinking agents which bind to the major groove of duplex dna
EP0463712A3 (en) * 1990-06-27 1992-04-08 University Patents, Inc. Polynucleotide phosphorodithioates as therapeutic agents for retroviral infections
US5734022A (en) * 1990-08-01 1998-03-31 The Johns Hopkins University Antibodies to a novel mammalian protein associated with uncontrolled cell division
WO1992009705A1 (en) * 1990-11-23 1992-06-11 Gilead Sciences, Inc. Triplex-forming oligomers containing modified bases
WO1992011390A1 (en) * 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Nucleic acid sequence detection by triple helix formation
US5683874A (en) * 1991-03-27 1997-11-04 Research Corporation Technologies, Inc. Single-stranded circular oligonucleotides capable of forming a triplex with a target sequence
US6136601A (en) * 1991-08-21 2000-10-24 Epoch Pharmaceuticals, Inc. Targeted mutagenesis in living cells using modified oligonucleotides
JPH06510203A (ja) * 1991-08-30 1994-11-17 ザ・ジョーンズ・ホプキンス・ユニバーシティ プリン塩基類似体を含むヌクレオシドオリゴマーを用いる二重鎖dnaの三重らせん複合体の形成
IL103311A0 (en) * 1991-10-07 1993-03-15 Univ Johns Hopkins Formation of triple helix complexes of single stranded nucleic acids using nucleoside oligomers
WO1993009127A1 (en) * 1991-11-07 1993-05-13 Gilead Sciences, Inc. Enhanced triple-helix formation directed by oligonucleotides composed of 2'-deoxy-7-deazaxanthosine and 2'-deoxy-7-deazaguanosine and related analogs
US5594121A (en) * 1991-11-07 1997-01-14 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
US6235887B1 (en) 1991-11-26 2001-05-22 Isis Pharmaceuticals, Inc. Enhanced triple-helix and double-helix formation directed by oligonucleotides containing modified pyrimidines
TW393513B (en) * 1991-11-26 2000-06-11 Isis Pharmaceuticals Inc Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
AU3222793A (en) * 1991-11-26 1993-06-28 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5484908A (en) * 1991-11-26 1996-01-16 Gilead Sciences, Inc. Oligonucleotides containing 5-propynyl pyrimidines
US5616461A (en) * 1992-05-14 1997-04-01 Dana-Farber Cancer Institute Assay for antiviral activity using complex of herpesvirus origin of replication and cellular protein
US6403302B1 (en) 1992-09-17 2002-06-11 California Institute Of Technology Methods and compositions for triple helix formation
WO1994007367A1 (en) * 1992-09-29 1994-04-14 Apollon, Inc. Anti-viral oligomers that bind polypurine tracts of single-stranded rna or rna-dna hybrids
EP0674705A4 (de) * 1992-11-09 1997-11-26 Univ Jefferson Antisense-oligonukleotide zur hemmung der expression mutierter gene und wildtypgene des collagens.
WO1994013793A1 (en) * 1992-12-04 1994-06-23 Apollon, Inc. Compounds and methods for the treatment of leukemias
AU684774B2 (en) * 1992-12-08 1998-01-08 Genta Incorporated Formation of triple helix complexes using a novel motif
WO1994017092A1 (en) * 1993-01-26 1994-08-04 Microprobe Corporation Bifunctional crosslinking oligonucleotides adapted for linking to a desired gene sequence of invading organism or cell
US6323185B1 (en) 1993-04-23 2001-11-27 The United States Of America As Represented By The Department Of Health And Human Services Anti-viral guanosine-rich oligonucleotides and method of treating HIV
US6288042B1 (en) 1993-04-23 2001-09-11 Aronex Pharmaceuticals, Inc. Anti-viral guanosine-rich tetrad forming oligonucleotides
US5567604A (en) * 1993-04-23 1996-10-22 Aronex Pharmaceuticals, Inc. Anti-viral guanosine-rich oligonucleotides
KR960703170A (ko) 1993-06-23 1996-06-19 알버트 디. 프리센. 안티센스 올리고뉴클레오티드 및 인간면역결핍바이러스감염에서 그것의 치료적이용(antisense oligonucleotides and therapeutic use thereof in human immunodeficiency virus infection)
US5962426A (en) * 1993-06-25 1999-10-05 Yale University Triple-helix forming oligonucleotides for targeted mutagenesis
ATE161541T1 (de) 1993-06-25 1998-01-15 Univ Yale Chemische-modifizierte oligonukleotide für site- spezifisches mutagenesis
WO1995011253A1 (en) * 1993-10-18 1995-04-27 The Walter And Eliza Hall Institute Of Medical Research A method for enhancing neurone survival and agents useful for same
US5488039A (en) * 1994-01-10 1996-01-30 Abbott Laboratories Method for the production of an enteral formula containing ribo-nucleotides
US5602109A (en) * 1994-01-10 1997-02-11 Abbott Laboratories Method to enhance the immune system of a human
US5492899A (en) * 1994-01-10 1996-02-20 Abbott Laboratories Infant nutritional formula with ribo-nucleotides
US5700590A (en) * 1994-01-10 1997-12-23 Abbott Laboratories Nutritional formula with ribo-nucleotides
US6136858A (en) * 1994-01-10 2000-10-24 Abbott Laboratories Infant formula and methods of improving infant stool patterns
US5808037A (en) * 1995-09-15 1998-09-15 Ramareddy Venkata Guntaka Oligomers which inhibit expression of collagen genes
US6156513A (en) * 1995-09-15 2000-12-05 Ramareddy V. Guntaka Oligmers which inhibit expression of collagen genes
US5780611A (en) * 1995-09-15 1998-07-14 Ramareddy Venkata Guntaka Oligomers which inhibit expression of collagen genes
FR2746412B1 (fr) * 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US6312925B1 (en) 1997-05-08 2001-11-06 Epoch Pharmaceuticals, Inc. Methods and compositions to facilitate D-loop formation by oligonucleotides
US6930175B1 (en) 1997-12-12 2005-08-16 The Johns Hopkins University School Of Medicine Gene family with transformation modulating activity
US8114850B2 (en) 1999-04-08 2012-02-14 Advanced Cancer Therapeutics, Llc Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
JP2002541264A (ja) * 1999-04-08 2002-12-03 ユーエイビー・リサーチ・ファウンデーション 冨gオリゴヌクレオチドの抗増殖活性およびヌクレオリンに結合するためのその使用方法
US20030114410A1 (en) 2000-08-08 2003-06-19 Technion Research And Development Foundation Ltd. Pharmaceutical compositions and methods useful for modulating angiogenesis and inhibiting metastasis and tumor fibrosis
FR2822156A1 (fr) * 2001-03-16 2002-09-20 Centre Nat Rech Scient Oligonucleotides pour la regulation du gene codant pour le tnf alpha et/ou de genes sous son controle et leurs applications
US8129514B2 (en) 2003-06-19 2012-03-06 Evogene Ltd. Nucleotide sequences for regulating gene expression in plant trichomes and constructs and methods utilizing same
EP1636333A4 (de) 2003-06-19 2007-10-24 Evogene Ltd Nukleotidsequenzen zur regulation der genexpression in pflanzentrichomen sowie diese verwendende konstrukte und verfahren
ES2435774T3 (es) 2005-07-07 2013-12-23 Yissum Research Development Company, Of The Hebrew University Of Jerusalem Agentes de ácido nucleico para la regulación negativa de H19, y métodos de uso del mismo
US8658608B2 (en) 2005-11-23 2014-02-25 Yale University Modified triple-helix forming oligonucleotides for targeted mutagenesis
WO2008087641A2 (en) 2007-01-16 2008-07-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem H19 silencing nucleic acid agents for treating rheumatoid arthritis
EP2200628A2 (de) 2007-07-15 2010-06-30 Yitzchak Hillman Behandlung von krankheiten über antimikrobielle peptide oder ihre inhibitoren
IL184627A0 (en) 2007-07-15 2008-12-29 Technion Res & Dev Foundation Agents for diagnosing and modulating metastasis and fibrosis as well as inflammation in a mammalian tissue
ES2700141T3 (es) 2007-08-02 2019-02-14 Gilead Biologics Inc Anticuerpos inhibidores LOXL2 y usos de los mismos
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
WO2009124150A2 (en) 2008-04-01 2009-10-08 Biosearch Technologies, Inc. Stabilized nucleic acid dark quencher-fluorophore probes
AU2009298802A1 (en) 2008-09-23 2010-04-08 Alnylam Pharmaceuticals, Inc. Chemical modifications of monomers and oligonucleotides with cycloaddition
WO2010080769A2 (en) 2009-01-06 2010-07-15 Arresto Biosciences, Inc. Chemotherapeutic methods and compositions
DK2414542T3 (da) 2009-03-30 2017-11-27 Tel Hashomer Medical Res Infrastructure & Services Ltd Fremgangsmåder til forudsigelse af klinisk forløb for og behandling af multipel sklerose
SG178845A1 (en) 2009-08-21 2012-04-27 Gilead Biologics Inc Catalytic domains from lysyl oxidase and loxl2
MX2012002189A (es) 2009-08-21 2012-03-16 Beeologics Inc Prevencion y cura de las enfermedades de los insectos beneficiosos a traves de moleculas.
WO2011030332A2 (en) 2009-09-08 2011-03-17 Yeda Research And Development Co. Ltd. Methods for hematopoietic precursor mobilization
WO2011030329A1 (en) 2009-09-10 2011-03-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method of treating tumors
EP3272869B1 (de) 2009-10-14 2020-06-24 Yissum Research Development Company of The Hebrew University of Jerusalem Ltd. Zusammensetzungen zur bekämpfung von varroa-milben bei bienen
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
BR112012019693A2 (pt) 2010-02-04 2017-06-20 Gilead Biologics Inc anticorpos que se ligam à lisil oxidase-like 2 (loxl2) e métodos de uso para eles.
RU2012148398A (ru) 2010-04-18 2014-05-27 Иеда Рисеч Энд Девелопмент Ко. Лтд. МОЛЕКУЛЫ И СПОСОБЫ ПРИМЕНЕНИЯ МОЛЕКУЛ ДЛЯ ЛЕЧЕНИЯ БОЛЕЗНЕЙ, АССОЦИИРОВАННЫХ С ErbB/ErbB ЛИГАНДАМИ
WO2011133868A2 (en) 2010-04-22 2011-10-27 Alnylam Pharmaceuticals, Inc. Conformationally restricted dinucleotide monomers and oligonucleotides
US10913767B2 (en) 2010-04-22 2021-02-09 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising acyclic and abasic nucleosides and analogs
WO2011158243A2 (en) 2010-06-16 2011-12-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method of diagnosing and treating cancer
CN103210082A (zh) 2010-09-15 2013-07-17 雷蒙特亚特特拉维夫大学有限公司 扩增胰岛β细胞和使其再分化的方法
EP2627766B1 (de) 2010-10-17 2016-05-25 Yeda Research and Development Co. Ltd. Verfahren und zusammensetzungen zur behandlung von insulinassoziierten erkrankungen
US9415103B2 (en) 2011-06-14 2016-08-16 Yeda Research And Development Co. Ltd. Combination therapy to prevent DCIS formation and progression to breast cancer
EP2741753A2 (de) 2011-08-09 2014-06-18 Yeda Research and Development Co. Ltd. Herunterregulierung von mir-7 zur förderung der beta-zellen-differenzierung und insulinproduktion
WO2013061328A2 (en) 2011-10-27 2013-05-02 Yeda Research And Development Co. Ltd. Method of treating cancer
US9617331B2 (en) 2011-11-27 2017-04-11 Yeda Research And Development Co. Ltd. Methods of regulating angiogenesis by administering agents which increase apoB-100 polypeptide
EP2844745A2 (de) 2012-02-22 2015-03-11 Brainstem Biotec Ltd. Erzeugung von neuralen stammzellen und motorneuronen
RU2015149680A (ru) 2013-04-21 2017-05-24 Йеда Ресеарч Энд Девелопмент Ко. Лтд. Агенты для подавления активности и/или снижения количества bcl-xl и/или bcl-w
RU2703498C2 (ru) 2013-07-19 2019-10-17 Монсанто Текнолоджи Ллс Композиции и способы борьбы с leptinotarsa
HUE070313T2 (hu) 2013-11-04 2025-05-28 Greenlight Biosciences Inc Ízeltlábú paraziták és kártevõfertõzöttség szabályozására szolgáló készítmények és eljárások
AU2015212341A1 (en) 2014-02-03 2016-09-08 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Use of Casein kinase I inhibitors for depleting stem cells
BR112016022711A2 (pt) 2014-04-01 2017-10-31 Monsanto Technology Llc composições e métodos para controle de pragas de inseto
RU2021123470A (ru) 2014-07-29 2021-09-06 Монсанто Текнолоджи Ллс Композиции и способы борьбы с насекомыми-вредителями
CN108064274A (zh) 2014-07-30 2018-05-22 耶达研究及发展有限公司 用于培养多能干细胞的培养基
WO2016030899A1 (en) 2014-08-28 2016-03-03 Yeda Research And Development Co. Ltd. Methods of treating amyotrophic lateral scleroses
WO2016079736A2 (en) 2014-11-17 2016-05-26 Yeda Research And Development Co. Ltd. Methods of treating diseases related to mitochondrial function
JP6942632B2 (ja) 2015-01-22 2021-09-29 モンサント テクノロジー エルエルシー Leptinotarsa防除用組成物及びその方法
EP3261582B1 (de) 2015-02-26 2021-01-06 Remodeless CV Ltd. Verfahren und zusammensetzungen in zusammenhang mit leptin-antagonisten
WO2016135732A1 (en) 2015-02-26 2016-09-01 Yeda Research And Development Co. Ltd. Method of promoting hair growth
CN107683089B (zh) 2015-05-04 2021-03-16 孟山都技术公司 用于控制节肢动物寄生虫和害虫侵扰的组合物和方法
EP3294280A1 (de) 2015-05-11 2018-03-21 Yeda Research and Development Co., Ltd. Citrinhemmer zur behandlung von krebs
WO2016185457A1 (en) 2015-05-19 2016-11-24 Yeda Research And Development Co. Ltd. Methods of promoting lymphangiogenesis
EA201792547A1 (ru) 2015-05-21 2018-04-30 Иеда Рисеч Энд Девелопмент Ко. Лтд. Популяции бактерий для стимуляции состояния здоровья
CA3205381A1 (en) 2015-07-17 2017-01-26 Alnylam Pharmaceuticals, Inc. Multi-targeted single entity conjugates
ES2965461T3 (es) 2015-08-03 2024-04-15 Biokine Therapeutics Ltd Inhibidor de CXCR4 para el tratamiento del cáncer
US11174484B2 (en) 2015-11-10 2021-11-16 B. G. Negev Technologies And Applications Ltd., At Ben- Gurion University Means and methods for reducing tumorigenicity of cancer stem cells
US10828318B2 (en) 2016-01-06 2020-11-10 Yeda Research And Development Co. Ltd. Compositions and methods for treating malignant, autoimmune and inflammatory diseases
JP2019508037A (ja) 2016-02-16 2019-03-28 イェール ユニバーシティーYale Universit 標的化遺伝子編集を増強するための組成物およびその使用方法
US20190216891A1 (en) 2016-03-06 2019-07-18 Yeda Research And Development Co., Ltd. Method for modulating myelination
IL247368A0 (en) 2016-08-18 2016-11-30 Yeda Res & Dev Diagnostic and therapeutic uses of exosomes
WO2018187493A1 (en) 2017-04-04 2018-10-11 Yale University Compositions and methods for in utero delivery
EP3658158A4 (de) 2017-07-27 2021-04-14 The National Institute for Biotechnology in the Negev Ltd. Smac/yldiablo-inhibitoren zur behandlung von krebs
IL255664A0 (en) 2017-11-14 2017-12-31 Shachar Idit Hematopoietic stem cells with enhanced properties
EP3723785A1 (de) 2017-12-17 2020-10-21 Yeda Research and Development Co. Ltd Komplexe modulatoren des cop9-signalosoms (csn) und deren verwendung
WO2019155465A1 (en) 2018-02-08 2019-08-15 Yeda Research And Development Co. Ltd. Methods of identifying and using agents for treating diseases associated with intestinal barrier dysfunction
US11648260B2 (en) 2018-03-29 2023-05-16 Technion Research And Development Foundation Limitted Vesicles comprising a PTEN inhibitor and uses of same
MX2020011570A (es) 2018-05-07 2020-11-24 Alnylam Pharmaceuticals Inc Administracion extrahepatica.
WO2020170240A1 (en) 2019-02-21 2020-08-27 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method for reduction drug-induced nephrotoxicity
AU2020279101B2 (en) 2019-05-17 2025-07-24 Alnylam Pharmaceuticals, Inc. Oral delivery of oligonucleotides
EP4004548B1 (de) 2019-07-29 2026-01-14 Yeda Research and Development Co. Ltd Verfahren zur behandlung und diagnose von lungenkrebs
WO2022011214A1 (en) 2020-07-10 2022-01-13 Alnylam Pharmaceuticals, Inc. Circular sirnas
WO2023283403A2 (en) 2021-07-09 2023-01-12 Alnylam Pharmaceuticals, Inc. Bis-rnai compounds for cns delivery
AU2022325816A1 (en) 2021-08-10 2024-03-21 Gamida-Cell Ltd. Engineered nk cells, methods of their production and uses thereof
EP4522742A2 (de) 2022-05-13 2025-03-19 Alnylam Pharmaceuticals, Inc. Einzelsträngige schleifenoligonukleotide
WO2024137756A1 (en) * 2022-12-23 2024-06-27 Hongene Biotech Corporation Compounds and methods for liquid phase oligonucleotide synthesis
EP4709855A2 (de) 2023-05-12 2026-03-18 Alnylam Pharmaceuticals, Inc. Einzelsträngige schleifenoligonukleotide

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EP0375408B1 (de) 1995-02-22
JPH04502407A (ja) 1992-05-07
EP0375408A1 (de) 1990-06-27
EP0449972A1 (de) 1991-10-09
PT92640A (pt) 1990-06-29
WO1990006934A1 (en) 1990-06-28
EP0449972A4 (en) 1992-03-18
DE68921315D1 (de) 1995-03-30
DE68921315T2 (de) 1995-08-03
AU4838490A (en) 1990-07-10
DK120091D0 (da) 1991-06-20
CA2006008C (en) 2000-02-15
ES2069598T3 (es) 1995-05-16
AU640910B2 (en) 1993-09-09
DK120091A (da) 1991-06-20
CA2006008A1 (en) 1990-06-20
PT92640B (pt) 1995-09-12

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