ATE344320T1 - Methode zur zielgerichteten evolution - Google Patents
Methode zur zielgerichteten evolutionInfo
- Publication number
- ATE344320T1 ATE344320T1 AT01965462T AT01965462T ATE344320T1 AT E344320 T1 ATE344320 T1 AT E344320T1 AT 01965462 T AT01965462 T AT 01965462T AT 01965462 T AT01965462 T AT 01965462T AT E344320 T1 ATE344320 T1 AT E344320T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- microcompartments
- concentration
- increasing
- amplification
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- 238000005382 thermal cycling Methods 0.000 abstract 1
- 239000007762 w/o emulsion Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Ecology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Electrotherapy Devices (AREA)
- Soil Working Implements (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0022458.4A GB0022458D0 (en) | 2000-09-13 | 2000-09-13 | Directed evolution method |
| US28377101P | 2001-04-13 | 2001-04-13 | |
| US28550101P | 2001-04-20 | 2001-04-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE344320T1 true ATE344320T1 (de) | 2006-11-15 |
Family
ID=27255886
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01965462T ATE344320T1 (de) | 2000-09-13 | 2001-09-13 | Methode zur zielgerichteten evolution |
| AT04078077T ATE396259T1 (de) | 2000-09-13 | 2001-09-13 | Methode zur zielgerichteten evolution |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04078077T ATE396259T1 (de) | 2000-09-13 | 2001-09-13 | Methode zur zielgerichteten evolution |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US7514210B2 (de) |
| EP (4) | EP1505151B1 (de) |
| JP (2) | JP5009481B2 (de) |
| AT (2) | ATE344320T1 (de) |
| AU (2) | AU2001286103B2 (de) |
| CA (2) | CA2585083A1 (de) |
| DE (2) | DE60124272T2 (de) |
| DK (1) | DK1317539T3 (de) |
| ES (2) | ES2274901T3 (de) |
| GB (1) | GB0022458D0 (de) |
| WO (1) | WO2002022869A2 (de) |
Families Citing this family (111)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2283936T3 (es) | 1997-07-07 | 2007-11-01 | Medical Research Council | Procedimiento de clasificacion in vitro. |
| GB9900298D0 (en) * | 1999-01-07 | 1999-02-24 | Medical Res Council | Optical sorting method |
| GB0127564D0 (en) * | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| FR2843404A1 (fr) * | 2002-08-07 | 2004-02-13 | Cellectis | Methode de determination de l'activite de clivage d'une endonuclease |
| CA2513889A1 (en) | 2003-01-29 | 2004-08-19 | 454 Corporation | Double ended sequencing |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
| GB0307403D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Selection by compartmentalised screening |
| US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
| CA2531105C (en) | 2003-07-05 | 2015-03-17 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| GB0325653D0 (en) * | 2003-11-03 | 2003-12-10 | Medical Res Council | CST emulsions |
| EP1801213A3 (de) | 2003-11-03 | 2007-10-24 | Medical Research Council | Polymerase |
| WO2005049787A2 (en) * | 2003-11-24 | 2005-06-02 | Yeda Research And Development Co.Ltd. | Compositions and methods for in vitro sorting of molecular and cellular libraries |
| ES2432040T3 (es) | 2004-01-28 | 2013-11-29 | 454 Life Sciences Corporation | Amplificación de ácido nucleico con emulsión de flujo continuo |
| US7417133B2 (en) * | 2004-02-27 | 2008-08-26 | Institut Pasteur | Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same |
| US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| CA2615323A1 (en) | 2005-06-06 | 2007-12-21 | 454 Life Sciences Corporation | Paired end sequencing |
| EP1743938A1 (de) * | 2005-07-12 | 2007-01-17 | NascaCell IP GmbH | Verfahren zur Oberflächenpräsentation von Polypeptiden |
| DE102005037351B3 (de) * | 2005-08-08 | 2007-01-11 | Geneart Ag | Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro |
| DE102005037349A1 (de) * | 2005-08-08 | 2007-02-15 | Geneart Ag | Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vivo |
| EP2363205A3 (de) | 2006-01-11 | 2014-06-04 | Raindance Technologies, Inc. | Mikrofluidische Vorrichtungen Und Verfahren Zur Verwendung Bei Der Bildung Und Kontrolle Von Nanoreaktoren |
| EP2530168B1 (de) | 2006-05-11 | 2015-09-16 | Raindance Technologies, Inc. | Mikrofluidische Vorrichtungen |
| US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
| CA2656022C (en) * | 2006-06-19 | 2017-10-17 | The Johns Hopkins University | Single-molecule pcr on microparticles in water-in-oil emulsions |
| EP2077912B1 (de) | 2006-08-07 | 2019-03-27 | The President and Fellows of Harvard College | Fluorkohlenstoffemulsionsstabilisierende tenside |
| EP2074211B1 (de) | 2006-09-06 | 2013-03-13 | Medical Research Council | Farbstoffmarkierte Nukleotidanalogen inkorporierende DNS Polymerasen |
| JP5457184B2 (ja) | 2006-09-14 | 2014-04-02 | ディエヌエイ・ポリメラーゼ・テクノロジー・インコーポレイテッド | Ｐｃｒ阻害物質の存在下におけるｄｎａ増幅のためのｔａｑポリメラーゼ変異体酵素の使用 |
| WO2008050104A1 (en) * | 2006-10-23 | 2008-05-02 | Medical Research Council | Polymerase |
| US7682791B2 (en) * | 2006-10-29 | 2010-03-23 | Macevicz Stephen C | Method of generating nested sets of double stranded DNA circles |
| US8772046B2 (en) | 2007-02-06 | 2014-07-08 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
| JP2010518863A (ja) | 2007-02-23 | 2010-06-03 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | インビトロ区画化を用いるタンパク質の選択および富化 |
| WO2008130623A1 (en) | 2007-04-19 | 2008-10-30 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
| AU2008329549B2 (en) | 2007-11-27 | 2013-08-22 | Human Genetic Signatures Pty Ltd | Enzymes for amplification and copying bisulphite modified nucleic acids |
| US8933320B2 (en) * | 2008-01-18 | 2015-01-13 | Tenksolar, Inc. | Redundant electrical architecture for photovoltaic modules |
| US8748727B2 (en) * | 2008-01-18 | 2014-06-10 | Tenksolar, Inc. | Flat-plate photovoltaic module |
| GB0806562D0 (en) | 2008-04-10 | 2008-05-14 | Fermentas Uab | Production of nucleic acid |
| EP2300613A4 (de) * | 2008-06-18 | 2011-11-09 | Life Technologies Corp | Mutierte und chemisch modifizierte thermisch stabile dna-polymerasen |
| US12038438B2 (en) | 2008-07-18 | 2024-07-16 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| WO2010009365A1 (en) | 2008-07-18 | 2010-01-21 | Raindance Technologies, Inc. | Droplet libraries |
| EP2326732A4 (de) * | 2008-08-26 | 2012-11-14 | Fluidigm Corp | Testverfahren für erhöhten durchsatz von proben und/oder targets |
| WO2010062777A2 (en) | 2008-11-03 | 2010-06-03 | Kapabiosystems | Modified type a dna polymerases |
| US8528589B2 (en) | 2009-03-23 | 2013-09-10 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
| GB0904957D0 (en) | 2009-03-23 | 2009-05-06 | Univ Erasmus Medical Ct | Tumour gene profile |
| DK2414547T3 (da) | 2009-04-02 | 2014-06-16 | Fluidigm Corp | Multiprimer-amplifikationsmetode til stregkodning af målnukleinsyrer |
| US8003329B2 (en) * | 2009-05-12 | 2011-08-23 | Becton Dickinson & Company | Molecular counting by color-coded micelles |
| EP2443666A4 (de) * | 2009-06-15 | 2013-06-05 | Tenksolar Inc | Beleuchtungsagnostische solartafel |
| JP2011051943A (ja) * | 2009-09-03 | 2011-03-17 | Tosoh Corp | タンパク質抽出試薬およびそれを用いたポリメラーゼのスクリーニング方法 |
| US10520500B2 (en) | 2009-10-09 | 2019-12-31 | Abdeslam El Harrak | Labelled silica-based nanomaterial with enhanced properties and uses thereof |
| US10207240B2 (en) * | 2009-11-03 | 2019-02-19 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
| EP2517025B1 (de) | 2009-12-23 | 2019-11-27 | Bio-Rad Laboratories, Inc. | Verfahren zur reduzierung des austauschs von molekülen zwischen tröpfchen |
| US10351905B2 (en) | 2010-02-12 | 2019-07-16 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
| JP5934657B2 (ja) | 2010-02-12 | 2016-06-15 | レインダンス テクノロジーズ， インコーポレイテッド | デジタル検体分析 |
| EP2569441B2 (de) * | 2010-05-14 | 2017-09-13 | cSens AB | Kit und verfahren zur messung von deoxynukleosidkinase-aktivität mittels dna-abhängiger dna-polymerase und natürlichen deoxynukleosiden |
| WO2012045012A2 (en) | 2010-09-30 | 2012-04-05 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| US9315787B2 (en) | 2011-01-14 | 2016-04-19 | Kapa Biosystems, Inc. | Modified DNA polymerases for improved amplification |
| EP3859011A1 (de) | 2011-02-11 | 2021-08-04 | Bio-Rad Laboratories, Inc. | Verfahren zur bildung gemischter tröpfchen |
| EP2675819B1 (de) | 2011-02-18 | 2020-04-08 | Bio-Rad Laboratories, Inc. | Zusammensetzungen und verfahren für molekulare etikettierung |
| US9074204B2 (en) | 2011-05-20 | 2015-07-07 | Fluidigm Corporation | Nucleic acid encoding reactions |
| DE202012013668U1 (de) | 2011-06-02 | 2019-04-18 | Raindance Technologies, Inc. | Enzymquantifizierung |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| WO2012170907A2 (en) | 2011-06-08 | 2012-12-13 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| DK2718260T3 (en) | 2011-06-08 | 2018-11-19 | Life Technologies Corp | DESIGN AND DEVELOPMENT OF NEW DETERGENTS FOR USE IN PCR SYSTEMS |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| JP6378085B2 (ja) | 2011-07-28 | 2018-08-22 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| GB201113430D0 (en) | 2011-08-03 | 2011-09-21 | Fermentas Uab | DNA polymerases |
| US20130040365A1 (en) | 2011-08-10 | 2013-02-14 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
| US11208636B2 (en) | 2011-08-10 | 2021-12-28 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
| GB201120711D0 (en) | 2011-12-01 | 2012-01-11 | Univ Erasmus Medical Ct | Method for classifying tumour cells |
| US20130157259A1 (en) * | 2011-12-15 | 2013-06-20 | Samsung Electronics Co., Ltd. | Method of amplifying dna from rna in sample and use thereof |
| CN108611398A (zh) | 2012-01-13 | 2018-10-02 | Data生物有限公司 | 通过新一代测序进行基因分型 |
| US9701959B2 (en) | 2012-02-02 | 2017-07-11 | Invenra Inc. | High throughput screen for biologically active polypeptides |
| US9840732B2 (en) | 2012-05-21 | 2017-12-12 | Fluidigm Corporation | Single-particle analysis of particle populations |
| EP3366769B1 (de) * | 2012-10-16 | 2021-02-17 | DNA Polymerase Technology, Inc. | Hemmungsresistente polymerasen |
| WO2014065760A1 (en) * | 2012-10-25 | 2014-05-01 | Agency For Science, Technology And Research | Methods for determining resistance against molecules targeting proteins |
| US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
| CN106458885B (zh) | 2013-10-25 | 2019-12-10 | 生命技术公司 | 用于聚合酶链式反应系统的新颖化合物及其应用 |
| US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
| EP3087088B1 (de) | 2013-12-23 | 2022-05-25 | University Of Rochester | Verfahren und zusammensetzungen zur ribosomalen synthese von makrocyclischen peptiden |
| US11193176B2 (en) | 2013-12-31 | 2021-12-07 | Bio-Rad Laboratories, Inc. | Method for detecting and quantifying latent retroviral RNA species |
| EP3795681B1 (de) * | 2014-12-16 | 2024-09-18 | Life Technologies Corporation | Polymerasezusammensetzungen und verfahren zur herstellung und verwendung davon |
| WO2016183294A1 (en) | 2015-05-12 | 2016-11-17 | Dna Polymerase Technology, Inc. | Mutant polymerases and uses thereof |
| US10647981B1 (en) | 2015-09-08 | 2020-05-12 | Bio-Rad Laboratories, Inc. | Nucleic acid library generation methods and compositions |
| CN108603224B (zh) | 2015-12-16 | 2022-06-28 | 富鲁达公司 | 高水平多路复用扩增 |
| JP6934477B2 (ja) | 2016-01-19 | 2021-09-15 | ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム | 熱安定逆転写酵素 |
| EP3497227A4 (de) | 2016-08-09 | 2020-10-07 | Integrated DNA Technologies, Inc. | Rnase-h-mutanten in einer emulsion |
| US12116570B2 (en) | 2016-09-29 | 2024-10-15 | Roche Sequencing Solutions, Inc. | Directed evolution of enzymes by plasmid tagging in droplets |
| CN110168102A (zh) * | 2016-11-15 | 2019-08-23 | 基因组影像公司 | 利用分子梳理监测经修饰的核酸酶诱导的基因编辑事件的方法 |
| CN108265039B (zh) * | 2016-12-30 | 2020-07-14 | 天津强微特生物科技有限公司 | 一种突变TaqDNA聚合酶及其纯化方法 |
| WO2018209092A1 (en) * | 2017-05-10 | 2018-11-15 | Board Of Regents, The University Of Texas System | Methods and devices related to amplifying nucleic acid at a variety of temperatures |
| WO2018232282A1 (en) * | 2017-06-15 | 2018-12-20 | Board Of Regents, The University Of Texas System | Methods and platform for screening and selecting metabolites and their receptors |
| LT6615B (lt) | 2017-09-12 | 2019-04-25 | Vilniaus Universitetas | N4-modifikuoti citidino nukleotidai ir jų panaudojimas |
| WO2019084538A1 (en) | 2017-10-27 | 2019-05-02 | Board Of Regents, The University Of Texas System | TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF |
| WO2019143622A1 (en) | 2018-01-19 | 2019-07-25 | Bio-Rad Laboratories, Inc. | Mutant dna polymerases |
| LT6742B (lt) | 2018-10-10 | 2020-07-10 | Vilnius University | Katalizinio biomolekulinio aktyvumo įrašymas į dnr seką |
| US11168312B2 (en) * | 2019-03-10 | 2021-11-09 | Abclonal Science, Inc. | Mutant taq polymerase for amplification in increased salt concentration or body fluids |
| US11613738B2 (en) * | 2019-05-14 | 2023-03-28 | Abclonal Science, Inc. | Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye |
| CN110218776A (zh) * | 2019-06-14 | 2019-09-10 | 苏州叠代生物科技有限公司 | Pcr油相组合物及其制备方法 |
| WO2021025623A1 (en) * | 2019-08-08 | 2021-02-11 | Agency For Science, Technology And Research | Method of detecting protein-protein interactions |
| US12270026B1 (en) | 2020-03-05 | 2025-04-08 | Ansa Biotechnologies, Inc. | Directed evolution method for template-independent polymerases |
| CN111621482B (zh) * | 2020-06-30 | 2022-04-29 | 浙江工业大学 | 一种草铵膦脱氢酶突变体、基因工程菌及一锅法多酶同步定向进化方法 |
| CN114480327B (zh) * | 2020-10-26 | 2024-01-16 | 厦门大学 | 一种Taq DNA聚合酶突变体 |
| CN117241878A (zh) | 2021-03-05 | 2023-12-15 | 伊努梅里斯公司 | 用于生成微滴和执行数字分析的系统和方法 |
| CN112695019B (zh) * | 2021-03-23 | 2021-06-25 | 翌圣生物科技（上海）有限公司 | 逆转录酶突变体及其应用 |
| JPWO2024009873A1 (de) * | 2022-07-08 | 2024-01-11 | ||
| WO2024211850A1 (en) * | 2023-04-05 | 2024-10-10 | Primrose Bio, Inc. | Methods and compositions for protein engineering |
| WO2024216275A2 (en) * | 2023-04-13 | 2024-10-17 | 5Prime Biosciences, Inc. | Compositions and methods for upregulation of reverse transcription and reduction of sequence bias in rna sequencing |
| DE102023001827A1 (de) | 2023-04-19 | 2024-10-24 | Horst Wochnowski | Verfahren und dazugehörige Vorrichtung zum Züchten von in Gewässern Mikroplastik zersetzenden und abbauenden Mikroorganismenstämme durch einen künstlich herbeigeführten und beschleunigten Evolutions- und Selektionsprozess |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5290479A (en) * | 1986-12-09 | 1994-03-01 | Phillips Petroleum Company | Surfactant system of polyethoxylated compounds and glyceride compounds |
| GB9107628D0 (en) * | 1991-04-10 | 1991-05-29 | Moonbrook Limited | Preparation of diagnostic agents |
| ES2130177T3 (es) * | 1991-08-10 | 1999-07-01 | Medical Res Council | Tratamiento de poblaciones de celulas. |
| US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5910408A (en) * | 1995-06-07 | 1999-06-08 | The General Hospital Corporation | Catalytic DNA having ligase activity |
| US6083693A (en) * | 1996-06-14 | 2000-07-04 | Curagen Corporation | Identification and comparison of protein-protein interactions that occur in populations |
| CA2236867A1 (en) * | 1996-09-27 | 1998-04-02 | Icos Corporation | Method to identify compounds for disrupting protein/protein interactions |
| WO1998023733A2 (en) * | 1996-11-27 | 1998-06-04 | University Of Washington | Thermostable polymerases having altered fidelity |
| CA2196496A1 (en) * | 1997-01-31 | 1998-07-31 | Stephen William Watson Michnick | Protein fragment complementation assay for the detection of protein-protein interactions |
| ES2283936T3 (es) * | 1997-07-07 | 2007-11-01 | Medical Research Council | Procedimiento de clasificacion in vitro. |
| EP1100889A4 (de) * | 1998-07-17 | 2002-03-06 | Mirus Corp | Micell systeme |
| GB9818432D0 (en) * | 1998-08-24 | 1998-10-21 | Bioline Limited | Thermostable DNA Polymerase |
| GB9900298D0 (en) * | 1999-01-07 | 1999-02-24 | Medical Res Council | Optical sorting method |
| GB0114854D0 (en) * | 2001-06-18 | 2001-08-08 | Medical Res Council | Selective gene amplification |
| GB0127564D0 (en) * | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
-
2000
- 2000-09-13 GB GBGB0022458.4A patent/GB0022458D0/en not_active Ceased
-
2001
- 2001-09-13 ES ES01965462T patent/ES2274901T3/es not_active Expired - Lifetime
- 2001-09-13 DE DE60124272T patent/DE60124272T2/de not_active Expired - Lifetime
- 2001-09-13 EP EP04078077A patent/EP1505151B1/de not_active Expired - Lifetime
- 2001-09-13 EP EP01965462A patent/EP1317539B1/de not_active Expired - Lifetime
- 2001-09-13 EP EP08103279A patent/EP1964932A3/de not_active Withdrawn
- 2001-09-13 CA CA002585083A patent/CA2585083A1/en not_active Abandoned
- 2001-09-13 DK DK01965462T patent/DK1317539T3/da active
- 2001-09-13 CA CA2421963A patent/CA2421963C/en not_active Expired - Lifetime
- 2001-09-13 AU AU2001286103A patent/AU2001286103B2/en not_active Expired
- 2001-09-13 DE DE60134181T patent/DE60134181D1/de not_active Expired - Lifetime
- 2001-09-13 WO PCT/GB2001/004108 patent/WO2002022869A2/en not_active Ceased
- 2001-09-13 ES ES04078077T patent/ES2304580T3/es not_active Expired - Lifetime
- 2001-09-13 EP EP06026714A patent/EP1806406A3/de not_active Withdrawn
- 2001-09-13 AT AT01965462T patent/ATE344320T1/de not_active IP Right Cessation
- 2001-09-13 AU AU8610301A patent/AU8610301A/xx active Pending
- 2001-09-13 AT AT04078077T patent/ATE396259T1/de not_active IP Right Cessation
- 2001-09-13 JP JP2002527309A patent/JP5009481B2/ja not_active Expired - Lifetime
-
2003
- 2003-03-13 US US10/387,387 patent/US7514210B2/en not_active Expired - Lifetime
-
2007
- 2007-03-09 JP JP2007061017A patent/JP2007190027A/ja active Pending
-
2008
- 2008-02-15 US US12/070,232 patent/US20080166772A1/en not_active Abandoned
-
2009
- 2009-02-25 US US12/392,412 patent/US20090246853A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20080166772A1 (en) | 2008-07-10 |
| EP1505151A2 (de) | 2005-02-09 |
| DK1317539T3 (da) | 2007-03-05 |
| EP1806406A3 (de) | 2009-07-29 |
| AU8610301A (en) | 2002-03-26 |
| GB0022458D0 (en) | 2000-11-01 |
| AU2001286103B2 (en) | 2006-08-10 |
| WO2002022869A2 (en) | 2002-03-21 |
| ES2274901T3 (es) | 2007-06-01 |
| EP1317539A2 (de) | 2003-06-11 |
| DE60124272T2 (de) | 2007-05-03 |
| JP5009481B2 (ja) | 2012-08-22 |
| EP1964932A3 (de) | 2009-05-20 |
| CA2421963A1 (en) | 2002-03-21 |
| DE60124272D1 (de) | 2006-12-14 |
| JP2007190027A (ja) | 2007-08-02 |
| ATE396259T1 (de) | 2008-06-15 |
| ES2304580T3 (es) | 2008-10-16 |
| CA2421963C (en) | 2013-04-09 |
| EP1806406A2 (de) | 2007-07-11 |
| US20090246853A1 (en) | 2009-10-01 |
| EP1317539B1 (de) | 2006-11-02 |
| JP2004508834A (ja) | 2004-03-25 |
| WO2002022869A3 (en) | 2002-10-17 |
| EP1505151A3 (de) | 2005-05-11 |
| DE60134181D1 (de) | 2008-07-03 |
| EP1505151B1 (de) | 2008-05-21 |
| EP1964932A2 (de) | 2008-09-03 |
| US7514210B2 (en) | 2009-04-07 |
| US20040005594A1 (en) | 2004-01-08 |
| CA2585083A1 (en) | 2002-03-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE60134181D1 (de) | Methode zur zielgerichteten Evolution | |
| ATE374815T1 (de) | Methode zur erhöhung der konzentration von nucleinsäuremolekülen | |
| ATE372391T1 (de) | Methode zur anreicherung von nukleinsäuremolekülen | |
| EA200300284A1 (ru) | Способ амплификации нуклеиновых кислот | |
| DE69636080D1 (de) | Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren | |
| PT1179600E (pt) | Monitorizacao da hibridacao durante a rcp | |
| ATE363530T1 (de) | Enzymstabilisierung durch kationische, oberflächenaktive substanzen | |
| NO941064D0 (no) | Selektiv restriksjonsfragmentamplifikasjon: en generell metode for DNA fingerprinting | |
| EP0975806A4 (de) | Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr) | |
| CY1106432T1 (el) | Μεθοδος και συσκευη για ενισχυση αλληλουχιων νουκλεϊνικου οξεως με χρηση μεταφορας θepμοτητος δια ρευματων | |
| ATE458068T1 (de) | Einzelnukleotidamplifikation und -nachweis mit polymerase | |
| ATE502116T1 (de) | Fluoreszierende proteine aus nicht- biolumineszenten arten der klasse anthozoa, gene die diese proteine kodieren und deren verwendungen | |
| ATE171221T1 (de) | Elektrochemische behandlung von nukleinsäure - enthaltenden lösungen | |
| ATE405674T1 (de) | Identizierung von microorganismen,die akute atemwegsinfektionen verursachen | |
| DK1026261T3 (da) | Forögelse af specificiteten af nukleinsyreamplifikation med bærernukleinsyre | |
| ATE269161T1 (de) | Kontaktierungsvorrichtung | |
| DE60228089D1 (de) | Nukleinsäureamplifikation mit duplexzwischenprodukten | |
| ES2109983T3 (es) | Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos. | |
| EP1170383A3 (de) | Oligonukleotide und Verfahren zur Bestimmung von SRSV RNA | |
| DE69833864D1 (de) | Methoden zur dns vervielfältigung und material dafür | |
| DE60207036D1 (de) | Detektionsverfahren für Escherichia coli | |
| ES2188357B1 (es) | Metodo para la amplificacion mediante transcripcion en reverso acoplada a una reaccion de nested o semi-nested pcr en un solo paso, de un arn diana, y sus aplicaciones en la identificacion de enterovirus y en la discriminacion entre poliovirus y enterovirus no-polio. | |
| ES2193861A1 (es) | Metodo para la identificacion molecular de fusarium oxysporum f. sp. ciceris y sus razas patogenicas 0,5 y 6 mediante pcr especifica. | |
| DE60142609D1 (de) | Methode zur Analyse doppelsträngiger DNA |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1317539 Country of ref document: EP |
|
| REN | Ceased due to non-payment of the annual fee |