ATE344320T1 - Methode zur zielgerichteten evolution - Google Patents
Methode zur zielgerichteten evolutionInfo
- Publication number
- ATE344320T1 ATE344320T1 AT01965462T AT01965462T ATE344320T1 AT E344320 T1 ATE344320 T1 AT E344320T1 AT 01965462 T AT01965462 T AT 01965462T AT 01965462 T AT01965462 T AT 01965462T AT E344320 T1 ATE344320 T1 AT E344320T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- microcompartments
- concentration
- increasing
- amplification
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- 238000005382 thermal cycling Methods 0.000 abstract 1
- 239000007762 w/o emulsion Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Ecology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Electrotherapy Devices (AREA)
- Soil Working Implements (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0022458.4A GB0022458D0 (en) | 2000-09-13 | 2000-09-13 | Directed evolution method |
| US28377101P | 2001-04-13 | 2001-04-13 | |
| US28550101P | 2001-04-20 | 2001-04-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE344320T1 true ATE344320T1 (de) | 2006-11-15 |
Family
ID=27255886
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01965462T ATE344320T1 (de) | 2000-09-13 | 2001-09-13 | Methode zur zielgerichteten evolution |
| AT04078077T ATE396259T1 (de) | 2000-09-13 | 2001-09-13 | Methode zur zielgerichteten evolution |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04078077T ATE396259T1 (de) | 2000-09-13 | 2001-09-13 | Methode zur zielgerichteten evolution |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US7514210B2 (de) |
| EP (4) | EP1806406A3 (de) |
| JP (2) | JP5009481B2 (de) |
| AT (2) | ATE344320T1 (de) |
| AU (2) | AU8610301A (de) |
| CA (2) | CA2585083A1 (de) |
| DE (2) | DE60124272T2 (de) |
| DK (1) | DK1317539T3 (de) |
| ES (2) | ES2274901T3 (de) |
| GB (1) | GB0022458D0 (de) |
| WO (1) | WO2002022869A2 (de) |
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| DE602004026033D1 (de) | 2003-01-29 | 2010-04-29 | 454 Corp | Zweiendige sequenzierung |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| GB0307403D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Selection by compartmentalised screening |
| GB0307428D0 (en) * | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
| US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
| US8048627B2 (en) | 2003-07-05 | 2011-11-01 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
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| US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
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| US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5910408A (en) * | 1995-06-07 | 1999-06-08 | The General Hospital Corporation | Catalytic DNA having ligase activity |
| US6083693A (en) * | 1996-06-14 | 2000-07-04 | Curagen Corporation | Identification and comparison of protein-protein interactions that occur in populations |
| CA2236867A1 (en) * | 1996-09-27 | 1998-04-02 | Icos Corporation | Method to identify compounds for disrupting protein/protein interactions |
| US6395524B2 (en) * | 1996-11-27 | 2002-05-28 | University Of Washington | Thermostable polymerases having altered fidelity and method of identifying and using same |
| CA2196496A1 (en) * | 1997-01-31 | 1998-07-31 | Stephen William Watson Michnick | Protein fragment complementation assay for the detection of protein-protein interactions |
| ATE358177T1 (de) * | 1997-07-07 | 2007-04-15 | Medical Res Council | Ein in vitro sortierverfahren |
| EP1100889A4 (de) * | 1998-07-17 | 2002-03-06 | Mirus Corp | Micell systeme |
| GB9818432D0 (en) * | 1998-08-24 | 1998-10-21 | Bioline Limited | Thermostable DNA Polymerase |
| GB9900298D0 (en) * | 1999-01-07 | 1999-02-24 | Medical Res Council | Optical sorting method |
| GB0114854D0 (en) * | 2001-06-18 | 2001-08-08 | Medical Res Council | Selective gene amplification |
| GB0127564D0 (en) * | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
-
2000
- 2000-09-13 GB GBGB0022458.4A patent/GB0022458D0/en not_active Ceased
-
2001
- 2001-09-13 WO PCT/GB2001/004108 patent/WO2002022869A2/en not_active Ceased
- 2001-09-13 CA CA002585083A patent/CA2585083A1/en not_active Abandoned
- 2001-09-13 DK DK01965462T patent/DK1317539T3/da active
- 2001-09-13 AT AT01965462T patent/ATE344320T1/de not_active IP Right Cessation
- 2001-09-13 ES ES01965462T patent/ES2274901T3/es not_active Expired - Lifetime
- 2001-09-13 JP JP2002527309A patent/JP5009481B2/ja not_active Expired - Lifetime
- 2001-09-13 AU AU8610301A patent/AU8610301A/xx active Pending
- 2001-09-13 EP EP06026714A patent/EP1806406A3/de not_active Withdrawn
- 2001-09-13 CA CA2421963A patent/CA2421963C/en not_active Expired - Lifetime
- 2001-09-13 AU AU2001286103A patent/AU2001286103B2/en not_active Expired
- 2001-09-13 EP EP01965462A patent/EP1317539B1/de not_active Expired - Lifetime
- 2001-09-13 AT AT04078077T patent/ATE396259T1/de not_active IP Right Cessation
- 2001-09-13 ES ES04078077T patent/ES2304580T3/es not_active Expired - Lifetime
- 2001-09-13 EP EP04078077A patent/EP1505151B1/de not_active Expired - Lifetime
- 2001-09-13 EP EP08103279A patent/EP1964932A3/de not_active Withdrawn
- 2001-09-13 DE DE60124272T patent/DE60124272T2/de not_active Expired - Lifetime
- 2001-09-13 DE DE60134181T patent/DE60134181D1/de not_active Expired - Lifetime
-
2003
- 2003-03-13 US US10/387,387 patent/US7514210B2/en not_active Expired - Lifetime
-
2007
- 2007-03-09 JP JP2007061017A patent/JP2007190027A/ja active Pending
-
2008
- 2008-02-15 US US12/070,232 patent/US20080166772A1/en not_active Abandoned
-
2009
- 2009-02-25 US US12/392,412 patent/US20090246853A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE60124272T2 (de) | 2007-05-03 |
| JP2004508834A (ja) | 2004-03-25 |
| AU2001286103B2 (en) | 2006-08-10 |
| EP1317539B1 (de) | 2006-11-02 |
| WO2002022869A3 (en) | 2002-10-17 |
| EP1806406A3 (de) | 2009-07-29 |
| CA2585083A1 (en) | 2002-03-21 |
| US7514210B2 (en) | 2009-04-07 |
| EP1505151B1 (de) | 2008-05-21 |
| EP1505151A3 (de) | 2005-05-11 |
| EP1505151A2 (de) | 2005-02-09 |
| JP2007190027A (ja) | 2007-08-02 |
| DE60124272D1 (de) | 2006-12-14 |
| US20080166772A1 (en) | 2008-07-10 |
| US20040005594A1 (en) | 2004-01-08 |
| AU8610301A (en) | 2002-03-26 |
| EP1317539A2 (de) | 2003-06-11 |
| CA2421963A1 (en) | 2002-03-21 |
| WO2002022869A2 (en) | 2002-03-21 |
| EP1964932A3 (de) | 2009-05-20 |
| ES2304580T3 (es) | 2008-10-16 |
| ES2274901T3 (es) | 2007-06-01 |
| GB0022458D0 (en) | 2000-11-01 |
| CA2421963C (en) | 2013-04-09 |
| DE60134181D1 (de) | 2008-07-03 |
| US20090246853A1 (en) | 2009-10-01 |
| JP5009481B2 (ja) | 2012-08-22 |
| DK1317539T3 (da) | 2007-03-05 |
| EP1806406A2 (de) | 2007-07-11 |
| EP1964932A2 (de) | 2008-09-03 |
| ATE396259T1 (de) | 2008-06-15 |
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