ATE346156T1 - Umkehrtransfektionsverfahren - Google Patents

Umkehrtransfektionsverfahren

Info

Publication number
ATE346156T1
ATE346156T1 AT00963550T AT00963550T ATE346156T1 AT E346156 T1 ATE346156 T1 AT E346156T1 AT 00963550 T AT00963550 T AT 00963550T AT 00963550 T AT00963550 T AT 00963550T AT E346156 T1 ATE346156 T1 AT E346156T1
Authority
AT
Austria
Prior art keywords
reverse transfection
transfection process
reverse
cells
arrays
Prior art date
Application number
AT00963550T
Other languages
English (en)
Inventor
David M Sabatini
Original Assignee
Whitehead Biomedical Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Whitehead Biomedical Inst filed Critical Whitehead Biomedical Inst
Application granted granted Critical
Publication of ATE346156T1 publication Critical patent/ATE346156T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Laminated Bodies (AREA)
  • Wrappers (AREA)
AT00963550T 1999-09-17 2000-09-18 Umkehrtransfektionsverfahren ATE346156T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15473799P 1999-09-17 1999-09-17
US19358000P 2000-03-30 2000-03-30

Publications (1)

Publication Number Publication Date
ATE346156T1 true ATE346156T1 (de) 2006-12-15

Family

ID=26851730

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00963550T ATE346156T1 (de) 1999-09-17 2000-09-18 Umkehrtransfektionsverfahren

Country Status (7)

Country Link
US (1) US6544790B1 (de)
EP (1) EP1218529B1 (de)
JP (1) JP2003509060A (de)
AT (1) ATE346156T1 (de)
CA (1) CA2383423A1 (de)
DE (1) DE60031997D1 (de)
WO (1) WO2001020015A1 (de)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6515009B1 (en) 1991-09-27 2003-02-04 Neorx Corporation Therapeutic inhibitor of vascular smooth muscle cells
US5811447A (en) 1993-01-28 1998-09-22 Neorx Corporation Therapeutic inhibitor of vascular smooth muscle cells
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
JP4303418B2 (ja) * 1997-10-24 2009-07-29 ライフ テクノロジーズ コーポレーション 組換え部位を有する核酸を使用する組換えクローニング
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US20050221280A1 (en) 1998-02-02 2005-10-06 Odyssey Thera, Inc. Protein-protein interactions for pharmacological profiling
US6990221B2 (en) * 1998-02-07 2006-01-24 Biodiscovery, Inc. Automated DNA array image segmentation and analysis
EP2119788A1 (de) * 1999-03-02 2009-11-18 Life Technologies Corporation Zusammensetzungen und Verfahren zur Verwendung beim Rekombinationsklonen von Nukleinsäuren
US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
US6731781B1 (en) * 1999-09-30 2004-05-04 Biodiscovery, Inc. System and method for automatically processing microarrays
US7099502B2 (en) * 1999-10-12 2006-08-29 Biodiscovery, Inc. System and method for automatically processing microarrays
CN1757724B (zh) * 1999-12-10 2014-06-11 茵维特罗根公司 具有独特特异性的多个重组位点在重组克隆中的用途
US8143195B2 (en) * 2000-01-24 2012-03-27 Yingjian Wang Arrays for bringing two or more reagents in contact with one or more biological targets and methods for making and using the arrays
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
AU2001284373A1 (en) * 2000-08-29 2002-03-13 Yeda Research And Development Co. Ltd. Methods of isolating genes encoding proteins of specific function and of screening for pharmaceutically active agents
US20050158702A1 (en) * 2000-09-05 2005-07-21 Stuelpnagel John R. Cellular arrays comprising encoded cells
JP2004537712A (ja) 2000-10-18 2004-12-16 バーチャル・アレイズ・インコーポレーテッド 多重細胞分析システム
EP1330516B1 (de) * 2000-11-03 2007-01-17 The Regents Of The University Of Michigan Oberflächentransfektion und expressionsverfahren
US6897067B2 (en) 2000-11-03 2005-05-24 Regents Of The University Of Michigan Surface transfection and expression procedure
US6902933B2 (en) * 2000-11-03 2005-06-07 Regents Of The University Of Michigan Surface transfection and expression procedure
US7029697B2 (en) * 2001-02-14 2006-04-18 Northwestern University Controlled surface-associated delivery of genes and oligonucleotides
US6890556B1 (en) * 2001-02-14 2005-05-10 Northwestern University Controlled surface-associated delivery of genes and oligonucleotides
US20030096414A1 (en) 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
US20070111201A1 (en) * 2001-04-30 2007-05-17 Benjamin Doranz Reverse transfection of cell arrays for structural and functional analyses of proteins
US6613083B2 (en) 2001-05-02 2003-09-02 Eckhard Alt Stent device and method
US20030100997A1 (en) * 2001-05-04 2003-05-29 Dunnington Damien J. Matrix assays in genomically indexed cells
JP2004532636A (ja) * 2001-05-21 2004-10-28 インヴィトロジェン コーポレーション 核酸分子の単離に用いるための組成物および方法
US6670129B2 (en) 2001-09-24 2003-12-30 Corning Incorporated Cell transfection apparatus and methods for making and using the cell transfection apparatus
US20030124549A1 (en) * 2001-10-11 2003-07-03 Xerox Corporation Devices and methods for detecting genetic sequences
AU2003213791A1 (en) * 2002-03-05 2003-09-22 Vitra Bioscience, Inc. Multiplexed cell transfection using coded carriers
JPWO2003100057A1 (ja) * 2002-05-28 2005-09-22 独立行政法人産業技術総合研究所 化学物質センサシステム
EP1527339B1 (de) 2002-06-03 2006-08-16 PamGene B.V. Hoch-durchsatz assay zur messung von zellulären antworten mit hilfe von mikroarrays
US7105347B2 (en) 2002-07-30 2006-09-12 Corning Incorporated Method and device for protein delivery into cells
JP3887588B2 (ja) * 2002-08-30 2007-02-28 株式会社リガク X線回折による応力測定法
US7512496B2 (en) * 2002-09-25 2009-03-31 Soheil Shams Apparatus, method, and computer program product for determining confidence measures and combined confidence measures for assessing the quality of microarrays
US7422853B1 (en) 2002-10-04 2008-09-09 Myriad Genetics, Inc. RNA interference using a universal target
US6905878B2 (en) * 2002-12-19 2005-06-14 The Scripps Research Institute DNA array for high throughput solid-phase transfection and method for producing the same
US20070269891A9 (en) * 2003-01-13 2007-11-22 Yasunobu Tanaka Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells
US20040138154A1 (en) * 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
US7691580B2 (en) * 2003-01-29 2010-04-06 Corning Incorporated Reverse protein delivery into cells on coded microparticles
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
US7883720B2 (en) * 2003-07-09 2011-02-08 Wisconsin Alumni Research Foundation Charge-dynamic polymers and delivery of anionic compounds
CN1860242B (zh) * 2003-08-01 2013-11-06 戴诺生物技术有限公司 自杂交多重靶核酸探针及其使用方法
US7488451B2 (en) 2003-09-15 2009-02-10 Millipore Corporation Systems for particle manipulation
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
EP1692521A1 (de) 2003-12-11 2006-08-23 Beth Israel Deaconess Medical Center, Inc. Rbp4 bei insulinempfindlichkeit/-resistenz, diabetes und obesität
EP2123759B1 (de) * 2004-02-06 2014-01-15 Thermo Fisher Scientific Biosciences Inc. Stabilisierte RNA als Transfektionskontrollen und Dämpfungsreagenzien
US20090280567A1 (en) * 2004-02-06 2009-11-12 Dharmacon, Inc. Stabilized sirnas as transfection controls and silencing reagents
US7125709B2 (en) * 2004-02-10 2006-10-24 Nitto Denko Corporation Culture device and method for eukaryotic cell transfection
CN100487122C (zh) * 2004-02-18 2009-05-13 日东电工株式会社 真核细胞转染的培养装置和方法
KR101147147B1 (ko) * 2004-04-01 2012-05-25 머크 샤프 앤드 돔 코포레이션 Rna 간섭의 오프 타겟 효과 감소를 위한 변형된폴리뉴클레오타이드
GB0410105D0 (en) * 2004-05-06 2004-06-09 Amersham Biosciences Uk Ltd Method for characterising compounds
KR101277646B1 (ko) * 2004-11-22 2013-06-25 다마콘, 아이엔씨. 건조 유전자 침묵 조성물을 포함하는 기구 및 시스템
US7935811B2 (en) * 2004-11-22 2011-05-03 Dharmacon, Inc. Apparatus and system having dry gene silencing compositions
US20060166234A1 (en) * 2004-11-22 2006-07-27 Barbara Robertson Apparatus and system having dry control gene silencing compositions
US7923207B2 (en) * 2004-11-22 2011-04-12 Dharmacon, Inc. Apparatus and system having dry gene silencing pools
US20060160169A1 (en) * 2004-12-03 2006-07-20 Board Of Regents, The University Of Texas System Cell microarray for profiling of cellular phenotypes and gene function
JP4739352B2 (ja) * 2004-12-17 2011-08-03 日東電工株式会社 真核細胞をトランスフェクトするための固定化分解性カチオンポリマーを持つ固体表面
US20080305961A1 (en) * 2005-02-03 2008-12-11 Abu Khabar Khalid S Method of Generating Translationally Active Linear Dna Molecules and Use Thereof in Array Formats
US20060223777A1 (en) * 2005-03-29 2006-10-05 Dharmacon, Inc. Highly functional short hairpin RNA
US20060257858A1 (en) * 2005-04-01 2006-11-16 Invitrogen Corporation Methods and compositions for introducing biopolymers into cells
EP1874950B1 (de) 2005-04-05 2015-06-03 Corning Incorporated Markierungsfreie biosensoren
US8734851B2 (en) * 2005-04-29 2014-05-27 Wisconsin Alumni Research Foundation Localized delivery of nucleic acid by polyelectrolyte assemblies
JP5105600B2 (ja) * 2005-05-17 2012-12-26 国立大学法人九州大学 核酸デリバリー方法
WO2007032761A1 (en) * 2005-09-09 2007-03-22 Jianhui Rong Cell-based microarrays and methods of use
WO2007042771A1 (en) * 2005-10-10 2007-04-19 Astrazeneca Ab Three hybrid screening assay using reverse transfected mammalian cell arrays
US20070161031A1 (en) * 2005-12-16 2007-07-12 The Board Of Trustees Of The Leland Stanford Junior University Functional arrays for high throughput characterization of gene expression regulatory elements
US8809514B2 (en) * 2006-09-22 2014-08-19 Ge Healthcare Dharmacon, Inc. Tripartite oligonucleotide complexes and methods for gene silencing by RNA interference
US7687267B2 (en) * 2006-09-30 2010-03-30 Rational Biotechnology Inc. High-throughput cell transfection device and methods of using thereof
EP2125220A1 (de) * 2006-12-13 2009-12-02 Qiagen GmbH Transfektions-mikroarrays
US8834918B2 (en) * 2007-01-22 2014-09-16 Wisconsin Alumni Research Foundation Modified multilayered film
US20080220983A1 (en) * 2007-03-08 2008-09-11 Switchgear Genomics A California Corporation Functional arrays for high throughput characterization of regulatory elements in untranslated regions of genes
JP5172845B2 (ja) * 2007-08-24 2013-03-27 株式会社サイトパスファインダー セリシンを用いたトランスフェクションデバイス
WO2009049100A2 (en) 2007-10-09 2009-04-16 Wisconsin Alumni Research Foundation Ultrathin multilayered films for controlled release of anionic reagents
US7845686B2 (en) * 2007-12-17 2010-12-07 S & B Technical Products, Inc. Restrained pipe joining system for plastic pipe
US8188060B2 (en) 2008-02-11 2012-05-29 Dharmacon, Inc. Duplex oligonucleotides with enhanced functionality in gene regulation
US8324333B2 (en) 2008-06-05 2012-12-04 Wisconsin Alumni Research Foundation Anionic charge-dynamic polymers for release of cationic agents
US8815779B2 (en) 2009-09-16 2014-08-26 SwitchGear Genomics, Inc. Transcription biomarkers of biological responses and methods
CN101787375B (zh) * 2010-02-10 2012-08-22 浙江大学 一种反向非病毒载体基因转染的方法
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9012375B2 (en) 2011-07-25 2015-04-21 Csir Array printing
US9029297B2 (en) * 2011-07-25 2015-05-12 Csir Array printing
KR101554448B1 (ko) 2011-07-25 2015-09-18 씨에스아이알 어레이 프린팅
US9593365B2 (en) 2012-10-17 2017-03-14 Spatial Transcriptions Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
LT2981616T (lt) * 2013-07-15 2018-11-26 Universität Heidelberg Baltymų ir nukleino rūgščių kietos fazės transfekavimas
EP3234103A4 (de) * 2014-12-15 2018-05-16 Novellusdx Ltd. Systeme, vorrichtungen, kits und verfahren zur indirekten transfektion von mehreren sets von nukleinsäuren und übertragung von molekülen
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
US20220290217A1 (en) 2018-12-10 2022-09-15 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
WO2022061150A2 (en) 2020-09-18 2022-03-24 10X Geonomics, Inc. Sample handling apparatus and image registration methods
EP4121555B1 (de) 2020-12-21 2026-02-25 10X Genomics, Inc. Verfahren zur erfassung von sonden und/oder barcodes
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (de) 2021-12-20 2024-10-09 10X Genomics, Inc. Selbsttest für eine pathologie-/histologie-objektträgerbildgebungsvorrichtung

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591567A (en) 1982-04-21 1986-05-27 California Institute Of Technology Recombinant DNA screening system including fixed array replicator and support
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
EP0769063A1 (de) 1994-06-27 1997-04-23 The Johns Hopkins University Gezielte freigabe genetischen materials
US5811274A (en) 1994-12-09 1998-09-22 The Regents Of The University Of Michigan Methods, compositions and apparatus for cell transfection
JP3867160B2 (ja) * 1995-07-03 2007-01-10 大日本住友製薬株式会社 遺伝子製剤
US5851818A (en) * 1996-05-31 1998-12-22 Sequus Pharmaceuticals, Inc. Condensed plasmid-liposome complex for transfection
US6133026A (en) 1996-05-31 2000-10-17 Sequus Pharmaceuticals, Inc. Condensed plasmid-liposome complex for transfection
WO1999055886A1 (en) 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Function-based gene discovery
DE1073771T1 (de) 1998-04-30 2002-06-13 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Neue methode zur selektion von klonen einer expressionsbibliothek mittels "rearraying"
WO1999057311A2 (en) 1998-04-30 1999-11-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Novel method for the identification of clones conferring a desired biological property from an expression library
GB9908681D0 (en) 1999-04-16 1999-06-09 Central Research Lab Ltd Apparatus for, and method of, introducing a substance into an object

Also Published As

Publication number Publication date
WO2001020015A1 (en) 2001-03-22
WO2001020015A9 (en) 2002-10-03
EP1218529B1 (de) 2006-11-22
EP1218529A1 (de) 2002-07-03
DE60031997D1 (de) 2007-01-04
JP2003509060A (ja) 2003-03-11
US6544790B1 (en) 2003-04-08
CA2383423A1 (en) 2001-03-22

Similar Documents

Publication Publication Date Title
DE60031997D1 (de) Umkehrtransfektionsverfahren
WO2002077264A3 (en) Arrayed transfection method and uses related thereto
EP0861262A4 (de) Gruppen zum einfangen von nukleinsäuren
DK1032705T3 (da) Probearrays og fremgangsmåder til at benytte probearrays til at skelne mellem DNA
AU1926899A (en) Exploiting genomics in the search for new drugs
ZA964776B (en) Mammalian cell culture process.
PL322611A1 (en) Methods of obtaining l-amini acid by fermentation, dna as well as micro-organism
AU2002225781A1 (en) Microincubator cell culture apparatus including transparent heater
NO994009L (no) Gensekvensator og metoder
ATE110415T1 (de) Verbessertes verfahren und apparat, um biologische substanzen in lebenden zellen einzuführen.
DE69620569D1 (de) Mikroventil und methode zu dessen herstellung, mikropumpe die dieses mikroventil benutzt und methode zu ihrer herstellung, sowie vorrichtung, die diese mikropumpe verwendet
NZ538852A (en) Selection and isolation of living cells using mRNA-binding probes
ID22863A (id) Metoda untuk memproduksi bahan asam nukleat
DK1085083T3 (da) Dyrkningsmedium for dyreceller og fremgangsmåde til proteinfremstilling under anvendelse af dette
ZA971158B (en) Nucleic acid constructs for the cell cycle-regulated expression of genes, cells containing such constructs and their use for preparing drugs.
ATE351902T1 (de) Oberflächentransfektion und expressionsverfahren
KR970002039A (ko) 급속발효 사료와 그 제조방법 및 용도
WO2002037944A3 (en) Multiplexed cell analysis system
NO993297D0 (no) Fast dyrkningsmedium for mikroorganismer, fremgangsmÕte for fremstilling herav og anvendelse herav
DE69930515D1 (de) Polyketide, ihre herstellung, und darin benutzte materialien
NO20000576D0 (no) Blanding og fremgangsmåte for regulering av celleproliferasjon og celledoed
DE69818482D1 (de) Physiologisch aktive substanzen tkr2449, verfahren zu ihrer herstellung und mikroorganismen
AU2003296973A1 (en) Dna array for high throughput solid-phase transfection and method for producing the same
AU5957100A (en) Novel mammalian calcium channels and related probes, cell lines and methods
TR199600902A2 (tr) Hücre transfeksiyonu.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties