ATE361313T1 - Allgemeines verfahren zur rückfaltung rekombinanter proteine - Google Patents
Allgemeines verfahren zur rückfaltung rekombinanter proteineInfo
- Publication number
- ATE361313T1 ATE361313T1 AT00988440T AT00988440T ATE361313T1 AT E361313 T1 ATE361313 T1 AT E361313T1 AT 00988440 T AT00988440 T AT 00988440T AT 00988440 T AT00988440 T AT 00988440T AT E361313 T1 ATE361313 T1 AT E361313T1
- Authority
- AT
- Austria
- Prior art keywords
- solution
- adjusted
- inclusion bodies
- proteins
- protein solution
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 238000007429 general method Methods 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 abstract 3
- 229960003180 glutathione Drugs 0.000 abstract 3
- 210000003000 inclusion body Anatomy 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 239000012460 protein solution Substances 0.000 abstract 3
- 239000000243 solution Substances 0.000 abstract 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 2
- 239000007983 Tris buffer Substances 0.000 abstract 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 abstract 2
- 235000003969 glutathione Nutrition 0.000 abstract 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 abstract 1
- 108010053070 Glutathione Disulfide Proteins 0.000 abstract 1
- 239000004471 Glycine Substances 0.000 abstract 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
- 238000002835 absorbance Methods 0.000 abstract 1
- 239000004202 carbamide Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000002523 gelfiltration Methods 0.000 abstract 1
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- ZNVGYHOBTCWGTO-UHFFFAOYSA-N solutin Natural products Cc1cc(O)cc2OC(C)(O)C(=O)c12 ZNVGYHOBTCWGTO-UHFFFAOYSA-N 0.000 abstract 1
- 238000000108 ultra-filtration Methods 0.000 abstract 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/10—Tetrapeptides
- C07K5/1021—Tetrapeptides with the first amino acid being acidic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17783600P | 2000-01-25 | 2000-01-25 | |
| US17836800P | 2000-01-27 | 2000-01-27 | |
| US21030600P | 2000-06-08 | 2000-06-08 | |
| US21029200P | 2000-06-08 | 2000-06-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE361313T1 true ATE361313T1 (de) | 2007-05-15 |
Family
ID=27497250
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00988440T ATE361313T1 (de) | 2000-01-25 | 2000-12-28 | Allgemeines verfahren zur rückfaltung rekombinanter proteine |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US6583268B2 (de) |
| EP (1) | EP1255769B1 (de) |
| JP (1) | JP3929777B2 (de) |
| AT (1) | ATE361313T1 (de) |
| AU (1) | AU783545B2 (de) |
| CA (1) | CA2397773C (de) |
| CY (1) | CY1106768T1 (de) |
| DE (1) | DE60034707T2 (de) |
| DK (1) | DK1255769T3 (de) |
| ES (1) | ES2287044T3 (de) |
| PT (1) | PT1255769E (de) |
| WO (1) | WO2001055174A2 (de) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1194449B1 (de) * | 1999-06-28 | 2010-09-22 | Oklahoma Medical Research Foundation | Inhibitoren des memapsin 2 und ihre verwendung |
| DK1255769T3 (da) * | 2000-01-25 | 2007-09-03 | Oklahoma Med Res Found | Universel procedure til genfoldning af rekombinante proteiner |
| US7186539B1 (en) * | 2001-08-31 | 2007-03-06 | Pharmacia & Upjohn Company | Method for refolding enzymes |
| ATE466020T1 (de) | 2001-11-09 | 2010-05-15 | Univ Georgetown | Neue isoform des gefässendothelzell wachstumshemmers (vegi) |
| US7166454B1 (en) * | 2002-05-24 | 2007-01-23 | Schering Corporation | Codon-optimized β-secretase and methods of refolding and processing |
| CN1311076C (zh) * | 2003-04-16 | 2007-04-18 | 普罗特奥姆技术公司 | 生产重组尿激酶的方法 |
| JP2004345958A (ja) * | 2003-05-13 | 2004-12-09 | Universal Bio Research Co Ltd | 蛋白質リフォールド装置および蛋白質リフォールド装置の使用方法 |
| US20050227920A1 (en) * | 2003-12-11 | 2005-10-13 | Xinli Lin | Methods for production of recombinant vascular endothelial cell growth inhibitor |
| EP1691764A2 (de) * | 2003-12-11 | 2006-08-23 | Proteomtech, Inc. | Verfahren zur behandlung von krebs mit vaskulärem endothelzell-wachstumshemmer oder vegi-192a |
| JP2005173484A (ja) * | 2003-12-15 | 2005-06-30 | Canon Inc | 画像形成装置及びプロセスカートリッジ |
| GB0405330D0 (en) * | 2004-03-10 | 2004-04-21 | Astrazeneca Ab | Enzyme and preparation method |
| US9756798B2 (en) | 2004-11-19 | 2017-09-12 | Patti D. Rubin | Burrow filling compressed growing medium |
| US20060107589A1 (en) | 2004-11-19 | 2006-05-25 | Rubin Patti D | Compressed growing medium |
| US20060130159A1 (en) * | 2004-12-09 | 2006-06-15 | Nick Masiello | Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum |
| WO2006081171A1 (en) | 2005-01-24 | 2006-08-03 | Amgen Inc. | Humanized anti-amyloid antibody |
| GB2446550B (en) * | 2005-11-28 | 2010-12-08 | Proteomtech Inc | Methods for production of recombinant alpha1-antitrypsin |
| MY143274A (en) | 2005-12-22 | 2011-04-15 | Genentech Inc | Recombinant production of heparin binding proteins |
| WO2007081534A2 (en) * | 2005-12-22 | 2007-07-19 | Pfizer Products Inc | Method of producing catalytically active bace2 enzymes |
| EP1845103B1 (de) * | 2006-04-10 | 2015-02-25 | Boehringer Ingelheim RCV GmbH & Co KG | Verfahren zur Proteinfaltung |
| KR100723531B1 (ko) * | 2006-06-13 | 2007-05-30 | 삼성전자주식회사 | 반도체 패키지 기판 |
| CN101506222B (zh) * | 2006-07-14 | 2013-10-02 | 健泰科生物技术公司 | 重组蛋白的重折叠 |
| WO2008054592A2 (en) * | 2006-09-29 | 2008-05-08 | Proteomtech, Inc. | Methods for production of recombinant plasminogen and plasmin polypeptides |
| US20100113355A1 (en) | 2007-04-27 | 2010-05-06 | Naresh Chennamsetty | Novel antibody molecules and nucleic acids binding to fungal stress protein hsp90 |
| WO2008132174A1 (en) * | 2007-04-27 | 2008-11-06 | Novartis Ag | An immunoglobulin composition |
| US20090113791A1 (en) | 2007-10-29 | 2009-05-07 | Oms Investments, Inc. | Compressed Coconut Coir Pith Granules and Methods for the Production and use Thereof |
| WO2009085200A2 (en) | 2007-12-21 | 2009-07-09 | Amgen Inc. | Anti-amyloid antibodies and uses thereof |
| KR101063347B1 (ko) | 2009-04-06 | 2011-09-07 | 인하대학교 산학협력단 | 이온성 액체를 이용한 이황화 결합을 포함하는 단백질의 재접힘 방법 |
| US20120142094A1 (en) | 2009-04-08 | 2012-06-07 | Ld Biopharma, Inc. | Generating ips cells by protein transduction of recombinant potency-determining factors |
| US20160039868A1 (en) * | 2013-03-22 | 2016-02-11 | Biogenomics Limited | Process for isolation and stabilisation of key intermediates for high efficiency refolding of recombinant proteins |
| US9982012B2 (en) | 2013-03-29 | 2018-05-29 | Dr. Reddy's Laboratories Limited | Refolding of granulocyte colony stimulating factor |
| WO2016201608A1 (zh) | 2015-06-15 | 2016-12-22 | 张鹏 | 可独立用于蛋白质复性或作为蛋白质复性前导操作的方法 |
| CN108822202B (zh) * | 2018-02-07 | 2021-11-30 | 电子科技大学 | 一种草鱼白细胞介素21重组蛋白及其制备方法 |
| CN116445462A (zh) * | 2023-04-20 | 2023-07-18 | 西安麦博泰克生物科技有限公司 | 一种重组猪胃蛋白酶的纯化制备方法及重组猪胃蛋白酶 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4518526A (en) * | 1982-12-22 | 1985-05-21 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
| US20020049303A1 (en) * | 1999-06-28 | 2002-04-25 | Tang Jordan J. N. | Catalytically active recombinant memapsin and methods of use thereof |
| EP1194449B1 (de) * | 1999-06-28 | 2010-09-22 | Oklahoma Medical Research Foundation | Inhibitoren des memapsin 2 und ihre verwendung |
| DK1255769T3 (da) * | 2000-01-25 | 2007-09-03 | Oklahoma Med Res Found | Universel procedure til genfoldning af rekombinante proteiner |
| ATE466020T1 (de) * | 2001-11-09 | 2010-05-15 | Univ Georgetown | Neue isoform des gefässendothelzell wachstumshemmers (vegi) |
| TW200417864A (en) * | 2003-03-11 | 2004-09-16 | Benq Corp | Electronic peripheral device and network interface card |
| WO2004094344A2 (en) | 2003-04-16 | 2004-11-04 | Proteomtech, Inc. | Methods for production of recombinant urokinase |
| CN1311076C (zh) * | 2003-04-16 | 2007-04-18 | 普罗特奥姆技术公司 | 生产重组尿激酶的方法 |
| EP1691764A2 (de) * | 2003-12-11 | 2006-08-23 | Proteomtech, Inc. | Verfahren zur behandlung von krebs mit vaskulärem endothelzell-wachstumshemmer oder vegi-192a |
| US20050227920A1 (en) * | 2003-12-11 | 2005-10-13 | Xinli Lin | Methods for production of recombinant vascular endothelial cell growth inhibitor |
-
2000
- 2000-12-28 DK DK00988440T patent/DK1255769T3/da active
- 2000-12-28 PT PT00988440T patent/PT1255769E/pt unknown
- 2000-12-28 CA CA002397773A patent/CA2397773C/en not_active Expired - Fee Related
- 2000-12-28 EP EP00988440A patent/EP1255769B1/de not_active Expired - Lifetime
- 2000-12-28 JP JP2001561026A patent/JP3929777B2/ja not_active Expired - Fee Related
- 2000-12-28 US US09/752,878 patent/US6583268B2/en not_active Expired - Fee Related
- 2000-12-28 ES ES00988440T patent/ES2287044T3/es not_active Expired - Lifetime
- 2000-12-28 DE DE60034707T patent/DE60034707T2/de not_active Expired - Fee Related
- 2000-12-28 WO PCT/US2000/035632 patent/WO2001055174A2/en not_active Ceased
- 2000-12-28 AU AU24649/01A patent/AU783545B2/en not_active Ceased
- 2000-12-28 AT AT00988440T patent/ATE361313T1/de not_active IP Right Cessation
-
2003
- 2003-04-17 US US10/420,044 patent/US7119166B2/en not_active Expired - Fee Related
-
2006
- 2006-08-18 US US11/506,739 patent/US20060287504A1/en not_active Abandoned
-
2007
- 2007-07-30 CY CY20071101003T patent/CY1106768T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1255769A2 (de) | 2002-11-13 |
| EP1255769B1 (de) | 2007-05-02 |
| US7119166B2 (en) | 2006-10-10 |
| WO2001055174A3 (en) | 2002-03-07 |
| ES2287044T3 (es) | 2007-12-16 |
| DE60034707D1 (de) | 2007-06-14 |
| JP3929777B2 (ja) | 2007-06-13 |
| CA2397773A1 (en) | 2001-08-02 |
| AU2464901A (en) | 2001-08-07 |
| US20030199676A1 (en) | 2003-10-23 |
| CA2397773C (en) | 2008-09-02 |
| AU783545B2 (en) | 2005-11-10 |
| US6583268B2 (en) | 2003-06-24 |
| JP2003523363A (ja) | 2003-08-05 |
| US20060287504A1 (en) | 2006-12-21 |
| US20010044521A1 (en) | 2001-11-22 |
| PT1255769E (pt) | 2007-07-23 |
| WO2001055174A2 (en) | 2001-08-02 |
| CY1106768T1 (el) | 2012-05-23 |
| DK1255769T3 (da) | 2007-09-03 |
| DE60034707T2 (de) | 2008-01-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1255769 Country of ref document: EP |
|
| REN | Ceased due to non-payment of the annual fee |