ATE362529T1 - Gewinnung von plasmiden in einem wässrigen zweiphasensystem - Google Patents

Gewinnung von plasmiden in einem wässrigen zweiphasensystem

Info

Publication number
ATE362529T1
ATE362529T1 AT03736399T AT03736399T ATE362529T1 AT E362529 T1 ATE362529 T1 AT E362529T1 AT 03736399 T AT03736399 T AT 03736399T AT 03736399 T AT03736399 T AT 03736399T AT E362529 T1 ATE362529 T1 AT E362529T1
Authority
AT
Austria
Prior art keywords
aqueous
polymer
plasmid dna
phase system
temperature
Prior art date
Application number
AT03736399T
Other languages
English (en)
Inventor
Cecilia Kepka
Jenny Rhodin
Folke Tjerneld
Original Assignee
Ge Healthcare Bio Sciences Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Bio Sciences Ab filed Critical Ge Healthcare Bio Sciences Ab
Application granted granted Critical
Publication of ATE362529T1 publication Critical patent/ATE362529T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03736399T 2002-08-27 2003-06-17 Gewinnung von plasmiden in einem wässrigen zweiphasensystem ATE362529T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0202552A SE0202552D0 (sv) 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system

Publications (1)

Publication Number Publication Date
ATE362529T1 true ATE362529T1 (de) 2007-06-15

Family

ID=20288830

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03736399T ATE362529T1 (de) 2002-08-27 2003-06-17 Gewinnung von plasmiden in einem wässrigen zweiphasensystem

Country Status (8)

Country Link
US (1) US7553658B2 (de)
EP (1) EP1532243B1 (de)
JP (1) JP4699757B2 (de)
AT (1) ATE362529T1 (de)
AU (1) AU2003237740A1 (de)
DE (1) DE60313870D1 (de)
SE (1) SE0202552D0 (de)
WO (1) WO2004020629A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT105438B (pt) * 2010-12-14 2014-08-14 Univ Do Minho Isolamento de dna plasmídico em sistemas de duas fases aquosas utilizando polímeros neutros derivatizados com grupos catiónicos

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008529516A (ja) * 2005-02-11 2008-08-07 インヴィトロジェン ダイナル エーエス エチレングリコール多量体の使用を含む核酸の単離方法
EP1762616A1 (de) * 2005-09-13 2007-03-14 Qiagen GmbH Verfahren zur selektiven Anreicherung von doppelsträngiger DNA aus Nukleinsäuregemischen
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
WO2008079302A2 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
JP2010530462A (ja) * 2007-06-19 2010-09-09 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー ポリマー２相システムおよびその使用
US8999702B2 (en) 2008-06-11 2015-04-07 Emd Millipore Corporation Stirred tank bioreactor
JP2012511929A (ja) 2008-12-16 2012-05-31 イー・エム・デイー・ミリポア・コーポレイシヨン 攪拌タンク反応器及び方法
WO2011146394A1 (en) 2010-05-17 2011-11-24 Millipore Corporation Stimulus responsive polymers for the purification of biomolecules
US9169379B2 (en) 2013-07-15 2015-10-27 King Fahd University Of Petroleum And Minerals Aqueous two-phase systems
EP4234710A3 (de) 2017-03-28 2023-11-01 Phase Scientific International, Ltd. Verfahren zur genauen diagnose von gegen krankheiten gerichtete biomarkers in der flüssigbiopsie
SG11201909324WA (en) 2017-06-01 2019-11-28 Yin To Chiu Phase separation behavior modifying agents for aqueous two-phase separation within porous material
WO2019046563A1 (en) 2017-09-01 2019-03-07 Phase Diagnostics, Inc. METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS
ES2964100T3 (es) 2017-09-18 2024-04-04 Phase Scient International Ltd Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos
US11479765B2 (en) 2018-01-19 2022-10-25 Phase Scientific International, Ltd. Method of isolating exosomes using encapsulation and aqueous micellar system
EP3740588A4 (de) 2018-01-19 2021-10-20 Phase Scientific International, Ltd. Verfahren zur isolierung und reinigung von nukleinsäuren unter verwendung eines fest-flüssig-phasensystems
WO2019143943A2 (en) * 2018-01-19 2019-07-25 Yin To Chiu Composition and method for concentration and enrichment of nucleic acids
WO2019143895A1 (en) 2018-01-19 2019-07-25 Yin To Chiu Spontaneous nucleic acid purification and concentration in a single step
EP4121530A1 (de) 2020-03-20 2023-01-25 Phase Scientific International, Ltd. Zusammensetzungen und verfahren zur ribonukleinsäureextraktion
CN112375109B (zh) * 2020-11-20 2023-03-28 华东理工大学 利用烷基酚聚氧乙烯聚氧丙烯醚npe-108分离纯化螺旋霉素
WO2023174022A1 (en) * 2022-03-16 2023-09-21 The University Of Hong Kong Synergistic control and dynamic assembly of viscoelastic networks and biomolecular condensates by aqueous liquid-liquid phase separation and liquid-solid phase separation (aqll-ls ps2)
EP4612288A1 (de) 2022-11-02 2025-09-10 Phase Scientific International, Ltd. Verfahren zur isolierung von zielanalyten aus biologischen proben unter verwendung von atps und festphasenmedien

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9603304D0 (sv) * 1996-09-11 1996-09-11 Pharmacia & Upjohn Ab Process for purifying a compound
JP2000513945A (ja) * 1996-09-11 2000-10-24 エスペリオン セラピューティクス インコーポレイテッド プロセスにおける使用のためにアポリポタンパク質および組成物を精製するためのプロセス

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT105438B (pt) * 2010-12-14 2014-08-14 Univ Do Minho Isolamento de dna plasmídico em sistemas de duas fases aquosas utilizando polímeros neutros derivatizados com grupos catiónicos

Also Published As

Publication number Publication date
US7553658B2 (en) 2009-06-30
AU2003237740A1 (en) 2004-03-19
JP4699757B2 (ja) 2011-06-15
JP2005537004A (ja) 2005-12-08
SE0202552D0 (sv) 2002-08-27
EP1532243A1 (de) 2005-05-25
EP1532243B1 (de) 2007-05-16
DE60313870D1 (de) 2007-06-28
US20060166349A1 (en) 2006-07-27
WO2004020629A1 (en) 2004-03-11

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