ATE373104T1 - Fluoreszenzsubstrate zum nachweis von organophosphatase-enzymaktivität - Google Patents

Fluoreszenzsubstrate zum nachweis von organophosphatase-enzymaktivität

Info

Publication number: ATE373104T1
Authority: AT; Austria
Prior art keywords: alkyl; alkoxy; group; aryl; formula
Prior art date: 2003-04-17

Application number

AT04721072T

Other languages

English (en)

Inventor

Serguei Soukharev

David Hammond

Original Assignee

American Nat Red Cross

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2003-04-17

Filing date

2004-03-16

Publication date

2007-09-15

2004-03-16 Application filed by American Nat Red Cross filed Critical American Nat Red Cross

2007-09-15 Application granted granted Critical

2007-09-15 Publication of ATE373104T1 publication Critical patent/ATE373104T1/de

Links

239000000758 substrate Substances 0.000 title 1
125000000217 alkyl group Chemical group 0.000 abstract 10
125000003545 alkoxy group Chemical group 0.000 abstract 5
125000003118 aryl group Chemical group 0.000 abstract 3
GJVFBWCTGUSGDD-UHFFFAOYSA-L pentamethonium bromide Chemical compound [Br-].[Br-].C[N+](C)(C)CCCCC[N+](C)(C)C GJVFBWCTGUSGDD-UHFFFAOYSA-L 0.000 abstract 3
125000000882 C2-C6 alkenyl group Chemical group 0.000 abstract 2
125000003601 C2-C6 alkynyl group Chemical group 0.000 abstract 2
125000004453 alkoxycarbonyl group Chemical group 0.000 abstract 2
125000004432 carbon atom Chemical group C* 0.000 abstract 2
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 abstract 2
125000004093 cyano group Chemical group *C#N 0.000 abstract 2
125000005843 halogen group Chemical group 0.000 abstract 2
125000001072 heteroaryl group Chemical group 0.000 abstract 2
125000005010 perfluoroalkyl group Chemical group 0.000 abstract 2
125000006700 (C1-C6) alkylthio group Chemical group 0.000 abstract 1
125000000171 (C1-C6) haloalkyl group Chemical group 0.000 abstract 1
125000006577 C1-C6 hydroxyalkyl group Chemical group 0.000 abstract 1
108091034117 Oligonucleotide Proteins 0.000 abstract 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 abstract 1
125000002777 acetyl group Chemical class [H]C([H])([H])C(*)=O 0.000 abstract 1
125000004442 acylamino group Chemical group 0.000 abstract 1
125000003282 alkyl amino group Chemical group 0.000 abstract 1
125000003368 amide group Chemical group 0.000 abstract 1
150000001413 amino acids Chemical class 0.000 abstract 1
125000005129 aryl carbonyl group Chemical group 0.000 abstract 1
125000000852 azido group Chemical group *N=[N+]=[N-] 0.000 abstract 1
125000003917 carbamoyl group Chemical class [H]N([H])C(*)=O 0.000 abstract 1
229910052799 carbon Inorganic materials 0.000 abstract 1
150000001875 compounds Chemical class 0.000 abstract 1
125000004966 cyanoalkyl group Chemical group 0.000 abstract 1
125000000753 cycloalkyl group Chemical group 0.000 abstract 1
125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 abstract 1
125000002485 formyl group Chemical group [H]C(*)=O 0.000 abstract 1
125000005347 halocycloalkyl group Chemical group 0.000 abstract 1
125000004970 halomethyl group Chemical group 0.000 abstract 1
125000002887 hydroxy group Chemical group [H]O* 0.000 abstract 1
125000005350 hydroxycycloalkyl group Chemical group 0.000 abstract 1
125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 abstract 1
125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
229910052760 oxygen Inorganic materials 0.000 abstract 1
125000004430 oxygen atom Chemical group O* 0.000 abstract 1
125000001476 phosphono group Chemical group [H]OP(*)(=O)O[H] 0.000 abstract 1
LFGREXWGYUGZLY-UHFFFAOYSA-N phosphoryl Chemical group [P]=O LFGREXWGYUGZLY-UHFFFAOYSA-N 0.000 abstract 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
102000004196 processed proteins & peptides Human genes 0.000 abstract 1
108090000765 processed proteins & peptides Proteins 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1
229920006395 saturated elastomer Polymers 0.000 abstract 1
125000000467 secondary amino group Chemical class [H]N([*:1])[*:2] 0.000 abstract 1
125000001424 substituent group Chemical group 0.000 abstract 1
229910052717 sulfur Inorganic materials 0.000 abstract 1
150000004043 trisaccharides Chemical class 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
- C07F9/6552—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a six-membered ring
- C07F9/65522—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a six-membered ring condensed with carbocyclic rings or carbocyclic ring systems
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Analytical Chemistry (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

AT04721072T 2003-04-17 2004-03-16 Fluoreszenzsubstrate zum nachweis von organophosphatase-enzymaktivität ATE373104T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US46331703P	2003-04-17	2003-04-17
US48793503P	2003-07-18	2003-07-18

Publications (1)

Publication Number	Publication Date
ATE373104T1 true ATE373104T1 (de)	2007-09-15

Family

ID=33313437

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT04721072T ATE373104T1 (de)	2003-04-17	2004-03-16	Fluoreszenzsubstrate zum nachweis von organophosphatase-enzymaktivität

Country Status (6)

Country	Link
US (2)	US7374900B2 (de)
EP (2)	EP1777297A1 (de)
AT (1)	ATE373104T1 (de)
DE (1)	DE602004008900T2 (de)
ES (1)	ES2297402T3 (de)
WO (1)	WO2004094658A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP4585766B2 (ja) *	2002-04-15	2010-11-24	アメリカンナショナルレッドクロス	混合物中のリガンドおよび標的を検出する方法
AU2006281012A1 (en) *	2005-08-17	2007-02-22	Yeda Research And Development Co. Ltd.	Methods and compositions for determining a level of biologically active serum paraoxonase
US8192685B2 (en)	2008-11-04	2012-06-05	Advanced Concepts And Technologies International, L.L.C.	Molecular separators, concentrators, and detectors preparatory to sensor operation, and methods of minimizing false positives in sensor operations
US9983184B2 (en)	2013-09-20	2018-05-29	Reflectonics, Inc.	Using fluorescence measurements for characterizing protein gel-firming processes

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
NL257864A (de) *	1959-11-13
DE1518898A1 (de) *	1965-12-08	1969-08-14	Bayer Ag	Verfahren zur Herstellung von Thiol- bzw. Dithiolphosphorsaeureestern
US4659657A (en) *	1982-12-24	1987-04-21	Bayer Aktiengesellschaft	Chromogenic and fluorogenic esters for photometric or fluorimetric determination of phosphatases or sulphatases
US5011964A (en) *	1985-03-07	1991-04-30	Cornell Research Foundation, Inc.	Novel diacylglycerophosphoric acid esters and use thereof as substrates in enzyme assays
JPH09262099A (ja) *	1996-03-28	1997-10-07	Aisin Seiki Co Ltd	フルオレセイン誘導体リン酸エステルによる核酸等の検出方法
US5773236A (en) *	1997-04-25	1998-06-30	Molecule Probes, Inc.	Assay for glutathiane transferase using polyhaloaryl-substituted reporter molecules
US5998593A (en) *	1998-03-10	1999-12-07	Abbott Laboratories	Fluorescent enzyme substrates
ATE241635T1 (de) *	1998-03-23	2003-06-15	Biosynth Ag	Neue potentiell fluorogene verbindungen
US5981207A (en) *	1998-12-18	1999-11-09	Pharmacopeia, Inc.	Caged enzyme substrates as probes for reporter enzyme activity
AUPR502301A0 (en) *	2001-05-15	2001-06-07	Commonwealth Scientific And Industrial Research Organisation	Phosphotriesterase from Agrobacterium radiobacter P230
US7052839B2 (en) *	2001-08-29	2006-05-30	Amersham Biosciences Corp	Terminal-phosphate-labeled nucleotides and methods of use
US7041812B2 (en) *	2001-08-29	2006-05-09	Amersham Biosciences Corp	Labeled nucleoside polyphosphates
JP4585766B2 (ja) *	2002-04-15	2010-11-24	アメリカンナショナルレッドクロス	混合物中のリガンドおよび標的を検出する方法

2004
- 2004-03-16 AT AT04721072T patent/ATE373104T1/de not_active IP Right Cessation
- 2004-03-16 DE DE602004008900T patent/DE602004008900T2/de not_active Expired - Fee Related
- 2004-03-16 WO PCT/US2004/007897 patent/WO2004094658A2/en not_active Ceased
- 2004-03-16 EP EP06025883A patent/EP1777297A1/de not_active Withdrawn
- 2004-03-16 ES ES04721072T patent/ES2297402T3/es not_active Expired - Lifetime
- 2004-03-16 US US10/553,650 patent/US7374900B2/en not_active Expired - Fee Related
- 2004-03-16 EP EP04721072A patent/EP1616025B1/de not_active Expired - Lifetime
2007
- 2007-01-03 US US11/648,679 patent/US20100255521A1/en not_active Abandoned

Also Published As

Publication number	Publication date
HK1086042A1 (en)	2006-09-08
DE602004008900T2 (de)	2008-06-12
WO2004094658A3 (en)	2005-01-13
US7374900B2 (en)	2008-05-20
WO2004094658A2 (en)	2004-11-04
US20070042370A1 (en)	2007-02-22
EP1616025B1 (de)	2007-09-12
US20100255521A1 (en)	2010-10-07
EP1616025A2 (de)	2006-01-18
DE602004008900D1 (de)	2007-10-25
EP1777297A1 (de)	2007-04-25
ES2297402T3 (es)	2008-05-01

Legal Events

Date	Code	Title	Description
2008-03-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

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KR970704697A (ko)	1997-09-06	2-[(디히드로)피라졸릴-3'-옥시메틸렌] 아닐리드의 살충제 및 살진균제로서의 용도 (Use of 2-[(Dihydro)Pyrazolyl-3'-Oxymethylene]-Anilides as Pest-Control Agents and Fungicides)
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EA200500718A1 (ru)	2005-10-27	Замещённые 6-(2-галогенфенил)триазолопиримидины
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