ATE375388T1 - Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden - Google Patents

Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden

Info

Publication number
ATE375388T1
ATE375388T1 AT02750301T AT02750301T ATE375388T1 AT E375388 T1 ATE375388 T1 AT E375388T1 AT 02750301 T AT02750301 T AT 02750301T AT 02750301 T AT02750301 T AT 02750301T AT E375388 T1 ATE375388 T1 AT E375388T1
Authority
AT
Austria
Prior art keywords
methods
mutagenesis
site
oligonucleotides
directed
Prior art date
Application number
AT02750301T
Other languages
English (en)
Inventor
Francesca Storici
Michael Resnick
Lysle Lewis
Original Assignee
Us Gov Health & Human Serv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv filed Critical Us Gov Health & Human Serv
Application granted granted Critical
Publication of ATE375388T1 publication Critical patent/ATE375388T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT02750301T 2001-07-27 2002-07-26 Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden ATE375388T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US30842601P 2001-07-27 2001-07-27

Publications (1)

Publication Number Publication Date
ATE375388T1 true ATE375388T1 (de) 2007-10-15

Family

ID=23193944

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02750301T ATE375388T1 (de) 2001-07-27 2002-07-26 Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden

Country Status (8)

Country Link
US (1) US7314712B2 (de)
EP (1) EP1421187B1 (de)
JP (1) JP2005500841A (de)
AT (1) ATE375388T1 (de)
AU (1) AU2002319696B2 (de)
CA (1) CA2455013A1 (de)
DE (1) DE60222914T2 (de)
WO (1) WO2003012036A2 (de)

Families Citing this family (237)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPR666501A0 (en) * 2001-07-27 2001-08-23 Murdoch Childrens Research Institute, The A method for selection and agents useful for same
US20050162122A1 (en) * 2004-01-22 2005-07-28 Dunn Glenn M. Fuel cell power and management system, and technique for controlling and/or operating same
WO2006032426A2 (en) * 2004-09-23 2006-03-30 Basf Plant Science Gmbh Recombination cassettes and methods for sequence excision in plants
US20070042404A1 (en) * 2005-08-19 2007-02-22 Huimin Zhao Compositions and method for detecting endonuclease activity
ES2582091T3 (es) 2005-10-18 2016-09-09 Precision Biosciences Meganucleasas diseñadas racionalmente con especificidad de secuencia y afinidad de unión a ADN alteradas
US20100285993A1 (en) * 2006-02-14 2010-11-11 Gregory Prelich Systematic Genomic Library and Uses Thereof
US20070281309A1 (en) * 2006-05-19 2007-12-06 Massachusetts Institute Of Technology Microfluidic-based Gene Synthesis
EP2059590B1 (de) 2006-07-14 2014-12-31 Novozymes, Inc. Verfahren zur produktion sezernierter polypeptide mit biologischer aktivität
US9267132B2 (en) * 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
JP2011505809A (ja) * 2007-12-07 2011-03-03 プレシジョン バイオサイエンシズ，インク． ヒトゲノムのＤＮａｓｅ高感受性領域に見出される認識配列を有する合理的に設計されたメガヌクレアーゼ
WO2009114321A2 (en) * 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
US20100071083A1 (en) * 2008-03-12 2010-03-18 Smith James J Temperature-dependent meganuclease activity
CA2722797A1 (en) * 2008-04-28 2009-11-05 Precision Biosciences, Inc. Fusion molecules of rationally-designed dna-binding proteins and effector domains
RU2545721C2 (ru) 2008-06-06 2015-04-10 Новозимс А/С Варианты семейства, состоящего из 44 ксилоглюканаз
PL3404088T3 (pl) 2008-06-06 2025-04-28 The Procter & Gamble Company Kompozycja detergentu zawierająca odmianę z grupy 44 ksyloglukanazy
EP2313498B1 (de) 2008-07-14 2017-03-15 Precision Biosciences, Inc. Erkennungssequenzen für meganukleasen aus i-crei und ihre verwendung
KR101722961B1 (ko) 2009-02-11 2017-04-04 알부메딕스 에이/에스 알부민 변이체 및 접합체
SG10201400436PA (en) * 2009-03-06 2014-06-27 Synthetic Genomics Inc Methods For Cloning And Manipulating Genomes
MX2012001060A (es) 2009-07-24 2012-03-14 Novozymes As Carbohidrato-oxidasas.
AU2010289725A1 (en) 2009-09-01 2012-04-05 Novozymes, Inc. Methods for improving malic acid production in filamentous fungi
CN102648273B (zh) 2009-09-25 2017-04-26 诺维信公司 枯草蛋白酶变体
CN102648277B (zh) 2009-09-25 2015-05-20 诺维信公司 蛋白酶变体的用途
BR112012006873A2 (pt) 2009-10-23 2015-09-08 Novozymes Inc variante isolada, polinucleotideo isolado, metodo para produzir uma variante, planta transgenica, parte de planta ou celula d eplanta, e, metodos para degradar ou converter um materialcelulósico, para produzir um produto de fermentação, e, para fermentar um material celulósico.
EP2493921B1 (de) 2009-10-30 2018-09-26 Albumedix Ltd Albuminvarianten
EP2507369A1 (de) 2009-12-03 2012-10-10 Novozymes A/S Varianten eines polypeptids mit lipolytischer aktivität und verbesserter stabilität
WO2011080353A1 (en) 2010-01-04 2011-07-07 Novozymes A/S Stabilization of alpha-amylases towards calcium depletion and acidic ph
WO2011104339A1 (en) 2010-02-25 2011-09-01 Novozymes A/S Variants of a lysozyme and polynucleotides encoding same
WO2011109524A2 (en) 2010-03-03 2011-09-09 Novozymes, Inc. Xylanase variants and polynucleotides encoding same
EP2556087A1 (de) 2010-04-09 2013-02-13 Novozymes Biopharma DK A/S Albuminderivate und varianten
US8835604B2 (en) 2010-06-12 2014-09-16 Adenium Biotech Aos Antimicrobial peptide variants and polynucleotides encoding same
US9512444B2 (en) * 2010-07-23 2016-12-06 Sigma-Aldrich Co. Llc Genome editing using targeting endonucleases and single-stranded nucleic acids
WO2012035103A1 (en) 2010-09-16 2012-03-22 Novozymes A/S Lysozymes
US10246691B2 (en) 2010-09-30 2019-04-02 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US9816082B2 (en) 2010-09-30 2017-11-14 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
ES2563209T3 (es) 2010-10-01 2016-03-11 Novozymes, Inc. Variantes de beta-glucosidasa y polinucleótidos que codifican las mismas
US20140080182A1 (en) 2011-03-31 2014-03-20 Novozymes, Inc. Cellulose Binding Domain Variants and Polynucleotides Encoding Same
AU2012249390B2 (en) 2011-04-27 2017-03-30 Amyris, Inc. Methods for genomic modification
CA2830660A1 (en) 2011-05-05 2012-11-08 Novozymes Biopharma Dk A/S Albumin variants
EP2540824A1 (de) 2011-06-30 2013-01-02 The Procter & Gamble Company Reinigungszusammensetzungen mit Amylasevariantenreferenz zu einem Sequenzprotokoll
US9434932B2 (en) 2011-06-30 2016-09-06 Novozymes A/S Alpha-amylase variants
BR122020009747B1 (pt) 2011-06-30 2021-07-20 Novozymes A/S Polipeptídeo e alfa-amilase variantes, composição detergente, e, utilização de uma alfa- amilase variante
CA2840962C (en) 2011-07-06 2021-04-13 Novozymes A/S Alpha amylase variants and polynucleotides encoding same
CN103827298A (zh) 2011-07-15 2014-05-28 诺维信公司 脂肪酶变体及其编码多核苷酸
CN103930543B (zh) 2011-09-06 2020-11-27 诺维信公司 葡糖淀粉酶变体和编码它们的多核苷酸
CA2849303C (en) 2011-09-30 2019-09-17 Novozymes, Inc. Dehydrogenase variants and polynucleotides encoding same
CA2853000C (en) 2011-10-11 2022-03-15 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
CN103857794B (zh) 2011-10-17 2018-03-20 诺维信公司 α‑淀粉酶变体以及编码它们的多核苷酸
EP3495479B1 (de) 2011-10-17 2025-08-06 Novozymes A/S Alpha-amylase-varianten und polynukleotide zur codierung davon
EP2780364A2 (de) 2011-11-18 2014-09-24 Eleven Biotherapeutics, Inc. Proteine mit verbesserter halbwertzeit und anderen eigenschaften
US10351834B2 (en) 2011-11-21 2019-07-16 Novozymes, Inc. GH61 polypeptide variants and polynucleotides encoding same
WO2013076269A1 (en) 2011-11-25 2013-05-30 Novozymes A/S Subtilase variants and polynucleotides encoding same
EP2607468A1 (de) 2011-12-20 2013-06-26 Henkel AG & Co. KGaA Reinigungsmittelzusammensetzungen mit Subtilasevarianten
JP2015504660A (ja) 2011-12-20 2015-02-16 ノボザイムス アクティーゼルスカブ サブチラーゼ変異体およびそれをコードするポリヌクレオチド
CA2878019A1 (en) 2011-12-20 2013-06-27 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
BR112014019142A2 (pt) 2012-02-03 2017-06-27 Procter & Gamble composições e métodos para tratamento de superfícies com lipases
CN104080908B (zh) 2012-02-03 2018-02-06 诺维信公司 脂肪酶变体以及编码它们的多核苷酸
EP2628785B1 (de) 2012-02-17 2016-05-18 Henkel AG & Co. KGaA Reinigungsmittelzusammensetzungen mit Subtilasevarianten
WO2013120948A1 (en) 2012-02-17 2013-08-22 Novozymes A/S Subtilisin variants and polynucleotides encoding same
DK2825556T3 (en) 2012-03-16 2018-04-16 Albumedix As albumin Variants
CN107988181A (zh) 2012-04-02 2018-05-04 诺维信公司 脂肪酶变体以及编码其的多核苷酸
US10227579B2 (en) 2012-04-27 2019-03-12 Novozymes A/S GH61 polypeptide variants and polynucleotides encoding same
CN104781412A (zh) 2012-07-20 2015-07-15 诺维信公司 5-羟甲基糠醛及其衍生物的酶氧化
MX2015001969A (es) 2012-08-17 2015-05-15 Novozymes As Variantes de asparaginasa termoestables y polinucleotidos que las codifican.
MX2015005363A (es) 2012-11-08 2015-11-06 Novozymes Biopharma Dk As Variantes de albumina.
WO2014102688A1 (en) * 2012-12-27 2014-07-03 Cellectis New design matrix for improvement of homology-directed gene targeting
EP2941485B1 (de) 2013-01-03 2018-02-21 Novozymes A/S Alpha-amylase-varianten und polynukleotide zur codierung davon
CN105164254B (zh) 2013-03-08 2019-06-28 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
WO2014147127A1 (en) 2013-03-21 2014-09-25 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
ES2674701T3 (es) 2013-04-30 2018-07-03 Novozymes A/S Variantes de glucoamilasa y polinucleótidos que las codifican
US9963690B2 (en) 2013-04-30 2018-05-08 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
EP4717756A2 (de) 2013-05-14 2026-04-01 Novozymes A/S Lipasevarianten und waschmittelzusammensetzungen
EP3004315A2 (de) 2013-06-06 2016-04-13 Novozymes A/S Alpha-amylase-varianten und polynukleotide zur codierung davon
US10378001B2 (en) 2013-06-27 2019-08-13 Novozymes A/S Subtilase variants and compositions comprising same
US20160145596A1 (en) 2013-06-27 2016-05-26 Novozymes A/S Subtilase Variants and Polynucleotides Encoding Same
ES2929143T3 (es) * 2013-07-09 2022-11-25 Harvard College Ingeniería genómica guiada por ARN múltiplex
WO2015004102A1 (en) 2013-07-09 2015-01-15 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
WO2015014803A1 (en) 2013-07-29 2015-02-05 Novozymes A/S Protease variants and polynucleotides encoding same
EP2832853A1 (de) 2013-07-29 2015-02-04 Henkel AG&Co. KGAA Waschmittelzusammensetzung mit Proteasevarianten
CN105358686A (zh) 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
WO2015049370A1 (en) 2013-10-03 2015-04-09 Novozymes A/S Detergent composition and use of detergent composition
WO2015059133A1 (en) 2013-10-22 2015-04-30 Novozymes A/S Cellobiose dehydrogenase variants and polynucleotides encoding same
WO2015079064A2 (en) 2013-11-29 2015-06-04 Novozymes A/S Peroxygenase variants
PT3080254T (pt) 2013-12-11 2024-07-30 Novozymes As Variantes cutinase e polinucleótidos que codificam o mesmo
US10208297B2 (en) 2014-01-22 2019-02-19 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same for cleaning
EP3097192B1 (de) 2014-01-22 2018-07-25 Novozymes A/S Pulluonasevarianten und polynukleotide zur codierung davon
EP3521434A1 (de) 2014-03-12 2019-08-07 Novozymes A/S Polypeptide mit lipaseaktivität und polynukleotide zur codierung davon
EP3129478B1 (de) 2014-04-10 2019-03-27 Novozymes A/S Alpha-amylase-varianten und sie kodierende polynukleotide
CN106661541B (zh) 2014-04-17 2021-09-03 贝林格尔·英格海姆Rcv两合公司 被工程化为过表达辅助蛋白的重组宿主细胞
AU2015248807B2 (en) 2014-04-17 2021-07-22 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell for expressing proteins of interest
AR100606A1 (es) 2014-05-27 2016-10-19 Novozymes As Variantes de lipasas y polinucleótidos que las codifican
CA2950273C (en) 2014-05-30 2022-06-21 Novozymes A/S Variants of gh family 11 xylanase and polynucleotides encoding same
EP2952584A1 (de) 2014-06-04 2015-12-09 Boehringer Ingelheim RCV GmbH & Co KG Verbesserte Proteinherstellung
WO2015189371A1 (en) 2014-06-12 2015-12-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
WO2015200659A1 (en) 2014-06-25 2015-12-30 Novozymes A/S Xylanase variants and polynucleotides encoding same
US10550381B2 (en) 2014-07-04 2020-02-04 Novozymes A/S Variant proteases and amylases having enhanced storage stability
EP3739029A1 (de) 2014-07-04 2020-11-18 Novozymes A/S Subtilasevarianten und polynukleotide zur codierung davon
PT3172227T (pt) 2014-07-21 2019-12-06 Delinia Inc Moléculas que ativam seletivamente células t reguladoras para o tratamento de doenças autoimuneis
AU2015301936B2 (en) 2014-08-11 2019-03-07 Delinia, Inc. Modified IL-2 variants that selectively activate regulatory T cells for the treatment of autoimmune diseases
EP3183339A1 (de) 2014-08-20 2017-06-28 Novozymes A/S Xyloglucanendotransglycosylasevarianten und polynukleotide zur codierung davon
EP3594335B1 (de) 2014-09-05 2024-05-01 Novozymes A/S Kohlenhydratbindende modulvarianten und polynukleotide zur codierung davon
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
WO2016079305A1 (en) 2014-11-20 2016-05-26 Novozymes A/S Alicyclobacillus variants and polynucleotides encoding same
WO2016090059A1 (en) 2014-12-02 2016-06-09 Novozymes A/S Laccase variants and polynucleotides encoding same
CA2963331C (en) 2014-12-04 2024-09-10 Novozymes A/S VARIANTS OF SUBTILASE AND THE POLYNUCLEOTIDES CODING FOR THESE
US10260024B2 (en) 2014-12-04 2019-04-16 Novozymes A/S Liquid cleaning compositions comprising protease variants
WO2016087401A1 (en) 2014-12-05 2016-06-09 Novozymes A/S Lipase variants and polynucleotides encoding same
US10400230B2 (en) 2014-12-19 2019-09-03 Novozymes A/S Protease variants and polynucleotides encoding same
US11518987B2 (en) 2014-12-19 2022-12-06 Novozymes A/S Protease variants and polynucleotides encoding same
DK3256598T3 (da) 2015-02-09 2021-01-11 Danisco Us Inc Svampestammer og anvendelsesfremgangsmåder
US10557127B2 (en) 2015-02-24 2020-02-11 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
AU2016259703B2 (en) 2015-05-08 2021-12-23 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP3294883A1 (de) 2015-05-08 2018-03-21 Novozymes A/S Alpha-amylase-varianten mit verbesserter leistung und stabilität
WO2016180749A1 (en) 2015-05-08 2016-11-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US10858637B2 (en) 2015-06-16 2020-12-08 Novozymes A/S Polypeptides with lipase activity and polynucleotides encoding same
EP4071244A1 (de) 2015-06-18 2022-10-12 Novozymes A/S Subtilasevarianten und polynukleotide zur codierung davon
MX394221B (es) 2015-07-01 2025-03-24 Novozymes As Metodos de reduccion de olor.
EP3950939A3 (de) 2015-07-06 2022-06-08 Novozymes A/S Lipasevarianten und polynukleotide zur codierung davon
GB201514034D0 (en) 2015-08-08 2015-09-23 Univ Plymouth Improvements in or relating to DNA recombination
JP7007261B2 (ja) 2015-08-20 2022-01-24 アルブミディクス リミティド アルブミン変異体及びコンジュゲート
EP4324919A3 (de) 2015-10-14 2024-05-29 Novozymes A/S Polypeptidvarianten
US10494685B2 (en) 2015-10-14 2019-12-03 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
WO2017070219A1 (en) 2015-10-20 2017-04-27 Novozymes A/S Lytic polysaccharide monooxygenase (lpmo) variants and polynucleotides encoding same
CN108291178B (zh) 2015-10-28 2020-08-04 诺维信公司 包含淀粉酶变体和蛋白酶变体的洗涤剂组合物
JP7025325B2 (ja) 2015-10-30 2022-02-24 ダニスコ・ユーエス・インク タンパク質発現の増強およびその方法
WO2017093318A1 (en) 2015-12-01 2017-06-08 Novozymes A/S Methods for producing lipases
US10035995B2 (en) 2015-12-07 2018-07-31 Eastman Chemical Company CALB variants
EP3394243A1 (de) 2015-12-22 2018-10-31 Albumedix Ltd. Verbesserte proteinexpressionsstämme
JP7146637B2 (ja) 2015-12-22 2022-10-04 アルブミディクス リミティド 改善されたタンパク質発現株
US11407986B2 (en) 2015-12-30 2022-08-09 Novozymes A/S Enzyme variants and polynucleotides encoding same
US20170204154A1 (en) 2016-01-20 2017-07-20 Delinia, Inc. Molecules that selectively activate regulatory t cells for the treatment of autoimmune diseases
MX2018008051A (es) 2016-01-29 2018-08-23 Novozymes As Variantes de beta-glucanasa y polinucleotidos que las codifican.
BR112018067371A2 (pt) 2016-03-02 2019-01-15 Novozymes As variante de celobio-hidrolase, composição de enzimas, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo, célula hospedeira recombinante, planta, parte de planta ou célula de planta transgênica, métodos para produção e para obtenção de uma variante de celobio-hidrolase, planta trangênica, e, processos para degradação de um material celulósico, para produção de um produto de fermentação e para fermentação de um material celulósico
US11965189B2 (en) 2016-03-24 2024-04-23 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
US10612032B2 (en) 2016-03-24 2020-04-07 The Board Of Trustees Of The Leland Stanford Junior University Inducible production-phase promoters for coordinated heterologous expression in yeast
CN109415421B (zh) 2016-05-03 2023-02-28 诺维信公司 α-淀粉酶变体以及编码它们的多核苷酸
CN109312319B (zh) 2016-05-09 2023-05-16 诺维信公司 具有改善的性能的变体多肽及其用途
US11293033B2 (en) 2016-05-18 2022-04-05 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
US10428327B2 (en) 2016-05-27 2019-10-01 Life Technologies Corporation Compositions and methods for enhancing homologous recombination
CN109715792A (zh) 2016-06-03 2019-05-03 诺维信公司 枯草杆菌酶变体和对其进行编码的多核苷酸
US11203732B2 (en) 2016-06-30 2021-12-21 Novozymes A/S Lipase variants and compositions comprising surfactant and lipase variant
WO2018002379A2 (en) 2016-07-01 2018-01-04 Novozymes A/S Enzymatic preparation of indigo dyes and in situ dyeing process
US10662417B2 (en) 2016-07-05 2020-05-26 Novozymes A/S Pectate lyase variants and polynucleotides encoding same
CA3029113A1 (en) 2016-07-08 2018-01-11 Novozymes A/S Xylanase variants and polynucleotides encoding same
CA3027272C (en) 2016-07-13 2022-06-21 The Procter & Gamble Company Bacillus cibi dnase variants and uses thereof
EP3485008B1 (de) 2016-07-18 2024-01-31 Novozymes A/S Lipasevarianten, polynukleotide zur codierung davon und verwendung davon
EP3487996B1 (de) 2016-07-21 2024-04-10 Novozymes A/S Serinprotease-varianten und polynukleotide zur codierung davon
EP3487995B1 (de) 2016-07-21 2021-05-26 Novozymes A/S Serinprotease-varianten und polynukleotide zur codierung davon
CN109563498A (zh) 2016-08-24 2019-04-02 汉高股份有限及两合公司 包含黄原胶裂解酶变体i的洗涤剂组合物
US11512300B2 (en) 2016-08-24 2022-11-29 Novozymes A/S Xanthan lyase variants and polynucleotides encoding same
WO2018037065A1 (en) 2016-08-24 2018-03-01 Henkel Ag & Co. Kgaa Detergent composition comprising gh9 endoglucanase variants i
CA3031609A1 (en) 2016-08-24 2018-03-01 Novozymes A/S Gh9 endoglucanase variants and polynucleotides encoding same
EP3538548A4 (de) 2016-11-08 2020-08-19 Delinia, Inc. Il-2-varianten zur behandlung von autoimmunerkrankungen
CN110268057B (zh) * 2016-11-16 2024-03-29 斯坦福大学托管董事会 用于鉴定和表达基因簇的系统和方法
EP3548615A1 (de) 2016-11-29 2019-10-09 Novozymes A/S Alpha-amylase-varianten und polynukleotide zur codierung davon
US12071643B2 (en) 2017-02-01 2024-08-27 Novozymes A/S Alpha-amylase variants
ES2965826T3 (es) 2017-02-01 2024-04-17 Procter & Gamble Composiciones limpiadoras que comprenden variantes de amilasa
CA3056920A1 (en) 2017-03-29 2018-10-04 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell with altered membrane lipid composition
BR112019021223A2 (pt) 2017-04-11 2020-04-28 Novozymes A/S variante de glucoamilase, métodos para aumentar a atividade de hidrólise de amido em bruto de uma glucoamilase e para produzir uma variante de glucoamilase, composição, uso de uma variante de glucoamilase, processos para produzir um produto de fermentação e para produzir um produto de xarope, polinucleotídeo codificando a variante de glucoamilase, construto de ácido nucleico, vetor de expressão, e, célula hospedeira.
WO2018202846A1 (en) 2017-05-05 2018-11-08 Novozymes A/S Compositions comprising lipase and sulfite
WO2018206535A1 (en) 2017-05-08 2018-11-15 Novozymes A/S Carbohydrate-binding domain and polynucleotides encoding the same
CA3058092A1 (en) 2017-05-08 2018-11-15 Novozymes A/S Mannanase variants and polynucleotides encoding same
WO2018206302A1 (en) 2017-05-08 2018-11-15 Novozymes A/S Mannanase variants and polynucleotides encoding same
US11130979B2 (en) 2017-06-20 2021-09-28 Albumedix Ltd Protein expression strains
US11499144B2 (en) 2017-06-22 2022-11-15 Novozymes A/S Xylanase variants and polynucleotides encoding same
US11220679B2 (en) 2017-08-08 2022-01-11 Novozymes A/S Polypeptides having trehalase activity
US11624059B2 (en) 2017-08-24 2023-04-11 Henkel Ag & Co. Kgaa Detergent compositions comprising GH9 endoglucanase variants II
CA3070749A1 (en) 2017-08-24 2019-02-28 Novozymes A/S Gh9 endoglucanase variants and polynucleotides encoding same
US11359188B2 (en) 2017-08-24 2022-06-14 Novozymes A/S Xanthan lyase variants and polynucleotides encoding same
US20210130744A1 (en) 2017-08-24 2021-05-06 Henkel Ag & Co. Kgaa Detergent composition comprising xanthan lyase variants ii
EP3679143A1 (de) 2017-09-08 2020-07-15 Life Technologies Corporation Verfahren zur verbesserten homologen rekombination und zusammensetzungen davon
EP4567094A3 (de) 2017-09-27 2026-01-07 Novozymes A/S Lipasevarianten und mikrokapselzusammensetzungen mit solchen lipasevarianten
JP7114697B2 (ja) 2017-09-27 2022-08-08 ザ プロクター アンド ギャンブル カンパニー リパーゼを含む洗剤組成物
HUE057832T2 (hu) 2017-10-27 2022-06-28 Procter & Gamble Polipeptid-variánsokat tartalmazó mosószerkészítmények
EP3701017A1 (de) 2017-10-27 2020-09-02 Novozymes A/S Dnase-varianten
EP3707252A1 (de) 2017-11-10 2020-09-16 Novozymes A/S Temperaturempfindliches cas9-protein
CN111670248A (zh) 2017-12-04 2020-09-15 诺维信公司 脂肪酶变体以及编码其的多核苷酸
DK3720955T5 (da) 2017-12-08 2024-08-26 Novozymes As Alfa-amylasevarianter og polynukleotider, der koder for dem
BR112020011173A2 (pt) 2017-12-08 2020-11-17 Novozymes A/S variante de alfa-amilase, polinucleotídeo, composição, construto de ácido nucleico, vetor de expressão, célula hospedeira, método de produção de uma variante de alfa-amilase, uso da variante, e, processo para produção de um xarope a partir de material contendo amido.
US12280118B2 (en) 2017-12-27 2025-04-22 Kyowa Kirin Co., Ltd. IL-2 variant
EP3749759A1 (de) 2018-02-08 2020-12-16 Novozymes A/S Lipasevarianten und zusammensetzungen davon
WO2019154951A1 (en) 2018-02-08 2019-08-15 Novozymes A/S Lipases, lipase variants and compositions thereof
CN111684056A (zh) 2018-02-28 2020-09-18 宝洁公司 清洁方法
EP3775190A1 (de) 2018-03-29 2021-02-17 Novozymes A/S Mannanase-varianten und dafür codierende polynukleotide
CN118460512A (zh) 2018-04-19 2024-08-09 诺维信公司 稳定化的纤维素酶变体
EP3781680A1 (de) 2018-04-19 2021-02-24 Novozymes A/S Stabilisierte cellulase-varianten
WO2019204767A1 (en) * 2018-04-20 2019-10-24 Dana-Farber Cancer Institute, Inc. Multifunctional nucleic acid reporter constructs
JP7645076B2 (ja) 2018-06-27 2025-03-13 ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 転写因子の使用によってタンパク質発現を増加させるための手段及び方法
CA3120107A1 (en) * 2018-11-30 2020-06-04 Geneinfosec, Inc. A method for generating random oligonucleotides and determining their sequence
JP7725365B2 (ja) 2019-03-21 2025-08-19 ノボザイムス アクティーゼルスカブ α－アミラーゼ変異体及びこれをコードするポリヌクレオチド
US12247237B2 (en) 2019-04-10 2025-03-11 Novozymes A/S Polypeptide variants
WO2020208056A1 (en) 2019-04-12 2020-10-15 Novozymes A/S Stabilized glycoside hydrolase variants
CN113939588A (zh) 2019-05-15 2022-01-14 诺维信公司 温度敏感性的rna指导的内切核酸酶
MX2021015382A (es) 2019-06-24 2022-01-24 Procter & Gamble Composiciones de limpieza que comprenden variantes de amilasa.
WO2020260223A1 (en) 2019-06-24 2020-12-30 Novozymes A/S Alpha-amylase variants
EP3994255A1 (de) 2019-07-02 2022-05-11 Novozymes A/S Lipasevarianten und zusammensetzungen davon
WO2021064068A1 (en) 2019-10-03 2021-04-08 Novozymes A/S Polypeptides comprising at least two carbohydrate binding domains
WO2021123184A2 (en) 2019-12-19 2021-06-24 Novozymes A/S Alpha-amylase variants
CA3160401C (en) 2019-12-19 2025-06-10 The Procter & Gamble Company CLEANING COMPOSITIONS COMPRISING POLYPEPTIDES HAVING ALPHA-AMYLASE ACTIVITY
JP2023506305A (ja) 2019-12-19 2023-02-15 ノボザイムス アクティーゼルスカブ キシラナーゼバリアント及びそれをコードするポリヌクレオチド
WO2021148278A1 (en) 2020-01-24 2021-07-29 Novozymes A/S Mutants of a filamentous fungal cell having increased productivity in the production of a polypeptide
EP4097227A1 (de) 2020-01-31 2022-12-07 Novozymes A/S Mannanase-varianten und dafür codierende polynukleotide
WO2021152120A1 (en) 2020-01-31 2021-08-05 Novozymes A/S Mannanase variants and polynucleotides encoding same
US20230142991A1 (en) 2020-02-10 2023-05-11 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US20230235367A1 (en) 2020-02-10 2023-07-27 Novozymes A/S Process for producing ethanol from raw starch using alpha-amylase variants
EP4110909A1 (de) 2020-02-26 2023-01-04 Novozymes A/S Polypeptidvarianten
WO2021204838A1 (en) 2020-04-08 2021-10-14 Novozymes A/S Carbohydrate binding module variants
WO2021242774A1 (en) * 2020-05-26 2021-12-02 Zymergen Inc. Methods of transient protein and gene expression in cells
EP4204551B1 (de) 2020-08-25 2025-09-17 Novozymes A/S Varianten einer familie44-xyloglucanase
WO2022043563A1 (en) 2020-08-28 2022-03-03 Novozymes A/S Polyester degrading protease variants
US20250346879A1 (en) 2020-10-07 2025-11-13 Novozymes A/S Alpha-amylase variants
JP2023547450A (ja) 2020-10-29 2023-11-10 ノボザイムス アクティーゼルスカブ リパーゼ変異体及びそのようなリパーゼ変異体を含む組成物
CN116438309A (zh) 2020-11-02 2023-07-14 诺维信公司 葡糖淀粉酶变体和编码它们的多核苷酸
EP4291646A2 (de) 2021-02-12 2023-12-20 Novozymes A/S Alpha-amylase-varianten
MX2023009756A (es) 2021-02-22 2023-09-04 Basf Se Variantes de amilasa.
EP4047088A1 (de) 2021-02-22 2022-08-24 Basf Se Amylasevarianten
EP4305146A1 (de) 2021-03-12 2024-01-17 Novozymes A/S Polypeptidvarianten
WO2022194673A1 (en) 2021-03-15 2022-09-22 Novozymes A/S Dnase variants
EP4060010A3 (de) 2021-03-15 2022-12-07 The Procter & Gamble Company Reinigungszusammensetzungen mit polypeptidvarianten
EP4060036A1 (de) 2021-03-15 2022-09-21 Novozymes A/S Polypeptidvarianten
WO2022249775A1 (ja) * 2021-05-27 2022-12-01 国立研究開発法人産業技術総合研究所 ゲノムを編集する方法
EP4416257A1 (de) 2021-10-12 2024-08-21 Novozymes A/S Endoglucanase mit verbesserter stabilität
WO2023247348A1 (en) 2022-06-21 2023-12-28 Novozymes A/S Mannanase variants and polynucleotides encoding same
EP4544015A2 (de) 2022-06-24 2025-04-30 Novozymes A/S Lipasevarianten und zusammensetzungen mit solchen lipasevarianten
WO2024033133A2 (en) 2022-08-11 2024-02-15 Basf Se Enzyme compositions comprising an amylase
JP2025526654A (ja) 2022-08-11 2025-08-15 ビーエーエスエフ ソシエタス・ヨーロピア プロテアーゼ、マンナナーゼ及び／又はセルラーゼを含む酵素組成物
AU2023400641A1 (en) 2022-11-30 2025-05-01 Novozymes A/S Carbonic anhydrase variants and polynucleotides encoding same
JP2026508744A (ja) 2022-12-05 2026-03-12 ノボザイムス アクティーゼルスカブ プロテアーゼバリアント、及びそれをコードするポリヌクレオチド
EP4629838A1 (de) 2022-12-08 2025-10-15 Novozymes A/S Polypeptid mit lysozymaktivität und polynukleotide zur codierung davon
CN120265743A (zh) 2022-12-14 2025-07-04 诺维信公司 改善的脂肪酶（gcl1）变体
EP4634389A1 (de) 2022-12-16 2025-10-22 Boehringer Ingelheim RCV GmbH & Co KG Mittel und verfahren zur erhöhten proteinexpression durch verwendung einer kombination von transportproteinen und chaperonen oder transkriptionsfaktoren
KR20250124113A (ko) 2022-12-16 2025-08-19 바스프 에스이 신규한 에틸렌디아민-n,n'-디숙신산 (edds) 신타아제
EP4389864A1 (de) 2022-12-20 2024-06-26 Basf Se Cutinasen
WO2025103765A1 (en) 2023-11-17 2025-05-22 Novozymes A/S Lytic polysaccharide monooxygenases and their use in detergent
WO2025111274A2 (en) 2023-11-20 2025-05-30 Novozymes A/S Epimerase variants and polynucleotides encoding same
WO2025168806A1 (en) 2024-02-08 2025-08-14 Novozymes A/S Polypeptides having carbonic anhydrase activity and polynucleotides encoding same
WO2025257254A1 (en) 2024-06-12 2025-12-18 Novozymes A/S Lipases and lipase variants and the use thereof
WO2026068782A1 (en) 2024-09-30 2026-04-02 Novozymes A/S Protease variants and polynucleotides encoding same

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4626504A (en) 1983-07-01 1986-12-02 Lubrizol Genetics, Inc. DNA transfer vector for gram-negative bacteria
WO1990014092A1 (en) * 1989-05-18 1990-11-29 Cell Genesys, Inc. Single-strand site-directed modification of mammalian genes in vivo
US5792632A (en) * 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5780296A (en) 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
US5776744A (en) * 1995-06-07 1998-07-07 Yale University Methods and compositions for effecting homologous recombination
US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US5942422A (en) 1996-11-14 1999-08-24 The Trustees Of Columbia University In The City Of New York Method for generating a directed, recombinant fusion nucleic acid
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
AU757930B2 (en) * 1997-12-01 2003-03-13 Roche Diagnostics Gmbh Optimization of cells for endogenous gene activation
US20020076688A1 (en) 2000-10-27 2002-06-20 Khodadoust Mehran M. Compositions and methods for the discovery and selection of biological information

Also Published As

Publication number Publication date
WO2003012036A2 (en) 2003-02-13
EP1421187A2 (de) 2004-05-26
US7314712B2 (en) 2008-01-01
DE60222914D1 (de) 2007-11-22
DE60222914T2 (de) 2008-07-24
EP1421187A4 (de) 2005-06-15
US20040171154A1 (en) 2004-09-02
JP2005500841A (ja) 2005-01-13
CA2455013A1 (en) 2003-02-13
WO2003012036A3 (en) 2004-02-12
AU2002319696B2 (en) 2007-11-01
EP1421187B1 (de) 2007-10-10

Similar Documents

Publication Publication Date Title
ATE375388T1 (de) Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden
TWI255853B (en) Method for modifying chromosomes
JPS5519092A (en) Selected protein producing method
DE69333112D1 (de) Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte
DE69930955D1 (de) Polypeptide mit lysophospholipaseaktivität und nukleinesäuren die sie kodieren
FR10C0025I1 (de)
WO2001029222A3 (en) Polypeptides having phospholipase b activity and nucleic acids encoding same
PL364138A1 (en) Proteins producing an altered immunogenic response and methods of making and using the same
YU51694A (sh) Postupak za dobijanje n-amino-1-hidroksi-alkilden-1,1-bifosfinskih kiselina
ATE284954T1 (de) Hyaluronan synthase gen und seine verwendung
EP1501935A4 (de) Kostengünstige herstellung von peptiden
ATE335086T1 (de) Künstliche chromosomen, die die genetische information für die bildung von rekombinantem virus enthalten
DE60025467D1 (de) Oxalacetat-hydrolase defiziente fungale wirtszellen
GB9825096D0 (en) Cells,culture methods and their uses
PL367306A1 (pl) Szczepionki na bazie autologicznych komórek T i sposoby ich otrzymywania
AU3382097A (en) Aspergillus oryzae 5-aminolevulinic acid synthases and nucleic acids encoding same
AU4627897A (en) Bordetella strain expressing the hybrid FHA, liposomes and vaccines
WO2000041509A3 (en) Polypeptides having acid phosphatase activity and nucleic acids encoding same
IL131103A0 (en) The neurotrophic factor nnt-1
WO2000004918A3 (de) Mittel zur immuntherapie von tumorerkrankungen
IL158223A0 (en) Peptidylarginine deiminase 6
KR100391884B1 (en) Random codon based mutagenesis using transposon
AU7645400A (en) Polypeptides having glucanotransferase activity and nucleic acids encoding same
ATE487735T1 (de) Säugetier milchwachstumsfaktor
Murakami et al. Random insertion and deletion mutagenesis for construction of protein library containing nonnatural amino acids

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties