ATE377074T1 - Methode zur bereitstellung von dna sequenzen - Google Patents

Methode zur bereitstellung von dna sequenzen

Info

Publication number
ATE377074T1
ATE377074T1 AT97924928T AT97924928T ATE377074T1 AT E377074 T1 ATE377074 T1 AT E377074T1 AT 97924928 T AT97924928 T AT 97924928T AT 97924928 T AT97924928 T AT 97924928T AT E377074 T1 ATE377074 T1 AT E377074T1
Authority
AT
Austria
Prior art keywords
activity
interest
dna sequences
structural gene
hybrid
Prior art date
Application number
AT97924928T
Other languages
English (en)
Inventor
Henrik Dalboege
Boerge Diderichsen
Thomas Sandal
Sakari Kauppinen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE377074T1 publication Critical patent/ATE377074T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1044Preparation or screening of libraries displayed on scaffold proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/248Xylanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811Selection methods for production or design of target specific oligonucleotides or binding molecules
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT97924928T 1996-05-10 1997-05-12 Methode zur bereitstellung von dna sequenzen ATE377074T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK56296 1996-05-10

Publications (1)

Publication Number Publication Date
ATE377074T1 true ATE377074T1 (de) 2007-11-15

Family

ID=8094949

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97924928T ATE377074T1 (de) 1996-05-10 1997-05-12 Methode zur bereitstellung von dna sequenzen

Country Status (7)

Country Link
US (1) US6270968B1 (de)
EP (1) EP0898618B1 (de)
AT (1) ATE377074T1 (de)
AU (1) AU3025597A (de)
DE (1) DE69738254T2 (de)
DK (1) DK0898618T3 (de)
WO (1) WO1997043409A2 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ505295A (en) * 1997-12-16 2002-12-20 Genencor Int A method of identifying cellulase gene sequences using conserved amino acid sequence fragments from known cellulase enzymes to provide primer nucleotide sequences
EP1123974B1 (de) * 1998-10-23 2010-05-05 Meiji Seika Kaisha Ltd. Endoglukanase und zellulase enthaltende präparate
JP2000253885A (ja) * 1999-03-11 2000-09-19 Jcr Pharmaceuticals Co Ltd 任意プライマーpcrを用いるdna断片クローニング方法
US7977051B2 (en) 1999-04-10 2011-07-12 Danisco Us Inc. EGIII-like enzymes, DNA encoding such enzymes and methods for producing such enzymes
DE10001140C2 (de) * 2000-01-13 2003-06-12 Gsf Forschungszentrum Umwelt Oligonukleotide zur Amplifikation und zum Nachweis von bakteriellen Genen für extrazelluläre alkalische Metallopeptidasen (Apr), neutrale Metallopeptidasen (Npr) und Serinpeptidasen (Spr)
DK1700917T3 (en) 2003-12-03 2016-07-25 Meiji Seika Pharma Co Ltd ENDOGLUCANASE STCE AND CELLULASE PREPARATION CONTAINING THE SAME
DK2330199T3 (en) * 2003-12-08 2015-05-18 Meiji Seika Pharma Co Ltd Surfactant-tolerant cellulase and its modification method
EP1612267A1 (de) * 2004-07-02 2006-01-04 GBF Gesellschaft für Biotechnologische Forschung mbH Zellulasen aus Rumen
US7741093B2 (en) 2005-04-29 2010-06-22 Ab Enzymes Oy Cellulases and their uses
WO2006117432A1 (en) * 2005-04-29 2006-11-09 Ab Enzymes Oy Improved cellulases
CN102282155B (zh) 2008-12-02 2017-06-09 日本波涛生命科学公司 磷原子修饰的核酸的合成方法
CA2747223C (en) 2008-12-23 2020-07-21 Danisco A/S Polypeptides with xylanase activity
RU2612521C2 (ru) 2009-07-06 2017-03-09 Онтории, Инк. Новые пролекарства нуклеиновых кислот и способы их применения
JP5868324B2 (ja) 2010-09-24 2016-02-24 株式会社Wave Life Sciences Japan 不斉補助基
CN103796657B (zh) 2011-07-19 2017-07-11 波涛生命科学有限公司 合成官能化核酸的方法
CA2879066C (en) 2012-07-13 2019-08-13 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant
PL2872485T3 (pl) 2012-07-13 2021-05-31 Wave Life Sciences Ltd. Asymetryczna grupa pomocnicza
KR102213609B1 (ko) 2012-07-13 2021-02-08 웨이브 라이프 사이언시스 리미티드 키랄 제어
US10322173B2 (en) 2014-01-15 2019-06-18 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
JPWO2015108048A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
JPWO2015108047A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤
BR112016016400A2 (pt) 2014-01-16 2017-10-03 Wave Life Sciences Ltd Composições de oligonucleotídeos quiralmente controlados, seu uso, sua composição farmacêutica, e métodos
EP3390625B1 (de) 2015-12-18 2023-09-06 Danisco US Inc. Polypeptide mit endoglucanaseaktivität und verwendungen davon
EP4525615A2 (de) 2022-05-14 2025-03-26 Novozymes A/S Zusammensetzungen und verfahren zur prävention, behandlung, unterdrückung und/oder beseitigung von phytopathogenen befällen und infektionen

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0368684B2 (de) * 1988-11-11 2004-09-29 Medical Research Council Klonierung von Immunglobulin sequenzen aus den variabelen Domänen.
US5780225A (en) * 1990-01-12 1998-07-14 Stratagene Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
DK0531372T4 (da) * 1990-05-09 2004-08-09 Novozymes As Cellulasepræparat omfattende et endoglucanaseenzym
DK115890D0 (da) * 1990-05-09 1990-05-09 Novo Nordisk As Enzym
MX9206979A (es) * 1991-12-04 1993-07-01 Novo Nordisk As Metodo de clonacion de proteinas en levaduras
US5364787A (en) * 1992-03-23 1994-11-15 Idaho Research Foundation Genes and enzymes involved in the microbial degradation of pentachlorophenol
ATE262035T1 (de) * 1992-10-06 2004-04-15 Novozymes As Zellulosevarianten
CN102080070B (zh) * 1995-03-17 2016-01-20 诺沃奇梅兹有限公司 新的内切葡聚糖酶
WO1997012991A1 (en) * 1995-09-22 1997-04-10 Terragen Diversity Inc. Method for isolating xylanase gene sequences from soil dna, compositions useful in such method and compositions obtained thereby

Also Published As

Publication number Publication date
DE69738254T2 (de) 2008-08-14
EP0898618A2 (de) 1999-03-03
DK0898618T3 (da) 2008-02-25
WO1997043409A3 (en) 1998-02-26
EP0898618B1 (de) 2007-10-31
DE69738254D1 (de) 2007-12-13
US6270968B1 (en) 2001-08-07
WO1997043409A2 (en) 1997-11-20
AU3025597A (en) 1997-12-05

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