ATE383421T1 - Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentien - Google Patents
Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentienInfo
- Publication number
- ATE383421T1 ATE383421T1 AT03793509T AT03793509T ATE383421T1 AT E383421 T1 ATE383421 T1 AT E383421T1 AT 03793509 T AT03793509 T AT 03793509T AT 03793509 T AT03793509 T AT 03793509T AT E383421 T1 ATE383421 T1 AT E383421T1
- Authority
- AT
- Austria
- Prior art keywords
- reversible
- parallel
- cloning method
- multitask
- corresponding reagents
- Prior art date
Links
- 238000010367 cloning Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 230000002441 reversible effect Effects 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 230000002068 genetic effect Effects 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Electrotherapy Devices (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
- Plural Heterocyclic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40848202P | 2002-09-03 | 2002-09-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE383421T1 true ATE383421T1 (de) | 2008-01-15 |
Family
ID=31978622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03793509T ATE383421T1 (de) | 2002-09-03 | 2003-09-03 | Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentien |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8318497B2 (de) |
| EP (1) | EP1556485B1 (de) |
| JP (1) | JP4546828B2 (de) |
| CN (1) | CN1703509B (de) |
| AT (1) | ATE383421T1 (de) |
| AU (1) | AU2003260200A1 (de) |
| CA (1) | CA2496514C (de) |
| DE (1) | DE60318603T2 (de) |
| WO (1) | WO2004022745A2 (de) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT395483B (de) * | 1989-01-13 | 1993-01-25 | Vaillant Gmbh | Rein elektrisch beheizter durchlauferhitzer mit vorrichtung zum ermitteln des wasserdurchsatzes |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1075512B1 (de) | 1998-05-07 | 2010-04-14 | Universite Libre De Bruxelles | Auf cytotoxinen basierendes biologisches einschlusssystem |
| CN100560724C (zh) | 2001-02-23 | 2009-11-18 | 布鲁塞尔自由大学 | 用于选择含有编码针对毒性分子的解毒剂蛋白的序列的重组克隆的方法 |
| EP2119789A1 (de) | 2008-05-16 | 2009-11-18 | Université Libre de Bruxelles | Hyperproliferative rekombinante Zelle |
| EP2612918A1 (de) | 2012-01-06 | 2013-07-10 | BASF Plant Science Company GmbH | In planta-Rekombination |
| BR122021015417B1 (pt) | 2013-08-19 | 2022-11-08 | Syngulon Sa | Célula microbiana geneticamente engenheirada que compreende um ácido nucleico que codifica uma bacteriocina |
| WO2016001447A1 (en) * | 2014-07-04 | 2016-01-07 | Universite Libre De Bruxelles | Method and system for the production of recombinant proteins by cells |
| EP3172334B1 (de) * | 2014-07-25 | 2021-11-10 | R.P. Scherer Technologies, LLC | Verbesserte wirtszelle zur herstellung von proteinen |
| WO2019121983A1 (en) | 2017-12-19 | 2019-06-27 | Syngulon S.A. | Fermentation process |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0273040B1 (de) * | 1986-03-26 | 1994-06-22 | GX BioSystems A/S | Biologische eindämmung |
| US5464764A (en) | 1989-08-22 | 1995-11-07 | University Of Utah Research Foundation | Positive-negative selection methods and vectors |
| US5300431A (en) * | 1991-02-26 | 1994-04-05 | E. I. Du Pont De Nemours And Company | Positive selection vector for the bacteriophage P1 cloning system |
| US7176029B2 (en) * | 1992-07-31 | 2007-02-13 | Universite Libre De Bruxelles | Cloning and/or sequencing vector |
| BE1006085A3 (fr) | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
| JP2590761B2 (ja) * | 1994-11-22 | 1997-03-12 | 日本電気株式会社 | Ｔａｂ式半導体装置およびｔａｂ式半導体装置を回路基板に接続する方法 |
| US6143557A (en) | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
| NZ312332A (en) * | 1995-06-07 | 2000-01-28 | Life Technologies Inc | Recombinational cloning using engineered recombination sites |
| CA2234557C (en) | 1995-10-13 | 2007-01-09 | Purdue Research Foundation | Method for the production of hybrid plants |
| US5922583A (en) | 1995-10-17 | 1999-07-13 | Biostar Inc. | Methods for production of recombinant plasmids |
| AU752704B2 (en) | 1997-10-24 | 2002-09-26 | Invitrogen Corporation | Recombinational cloning using nucleic acids having recombination sites |
| EP1075512B1 (de) | 1998-05-07 | 2010-04-14 | Universite Libre De Bruxelles | Auf cytotoxinen basierendes biologisches einschlusssystem |
| US6134557A (en) * | 1998-11-20 | 2000-10-17 | Matlink, Inc. | Materials and supplies ordering system |
| US6271359B1 (en) * | 1999-04-14 | 2001-08-07 | Musc Foundation For Research Development | Tissue-specific and pathogen-specific toxic agents and ribozymes |
| CA2388907A1 (en) | 1999-10-25 | 2001-05-03 | Life Technologies Corporation | Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination |
| DK1250453T3 (da) | 1999-12-10 | 2008-08-11 | Invitrogen Corp | Anvendelse af multiple rekombinations-sites med unik specificitet i rekombinationskloning |
| EP1111061A1 (de) * | 1999-12-20 | 2001-06-27 | Universite Libre De Bruxelles | Vektor mit doppelter Selektion |
| DE10038573A1 (de) | 2000-08-03 | 2002-02-21 | Mpb Cologne Gmbh Molecular Pla | Verfahren zur Selektion auf Wirtszellen mit eliminierten DNA-Sequenzen |
| CN100560724C (zh) * | 2001-02-23 | 2009-11-18 | 布鲁塞尔自由大学 | 用于选择含有编码针对毒性分子的解毒剂蛋白的序列的重组克隆的方法 |
| US6531153B2 (en) * | 2001-05-29 | 2003-03-11 | Drugtech Corporation | Composition with sustained release of levodopa and carbidopa |
| IL164131A0 (en) * | 2002-03-19 | 2005-12-18 | Univ Bruxelles | Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms |
-
2003
- 2003-09-03 AT AT03793509T patent/ATE383421T1/de not_active IP Right Cessation
- 2003-09-03 EP EP03793509A patent/EP1556485B1/de not_active Expired - Lifetime
- 2003-09-03 WO PCT/BE2003/000147 patent/WO2004022745A2/en not_active Ceased
- 2003-09-03 AU AU2003260200A patent/AU2003260200A1/en not_active Abandoned
- 2003-09-03 CN CN038233053A patent/CN1703509B/zh not_active Expired - Fee Related
- 2003-09-03 DE DE60318603T patent/DE60318603T2/de not_active Expired - Lifetime
- 2003-09-03 CA CA2496514A patent/CA2496514C/en not_active Expired - Fee Related
- 2003-09-03 JP JP2004533086A patent/JP4546828B2/ja not_active Expired - Fee Related
- 2003-09-03 US US10/526,525 patent/US8318497B2/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT395483B (de) * | 1989-01-13 | 1993-01-25 | Vaillant Gmbh | Rein elektrisch beheizter durchlauferhitzer mit vorrichtung zum ermitteln des wasserdurchsatzes |
Also Published As
| Publication number | Publication date |
|---|---|
| DE60318603T2 (de) | 2008-07-03 |
| JP4546828B2 (ja) | 2010-09-22 |
| CA2496514C (en) | 2012-11-06 |
| CA2496514A1 (en) | 2004-03-18 |
| AU2003260200A8 (en) | 2004-03-29 |
| CN1703509A (zh) | 2005-11-30 |
| AU2003260200A1 (en) | 2004-03-29 |
| CN1703509B (zh) | 2010-09-01 |
| US20060088841A1 (en) | 2006-04-27 |
| DE60318603D1 (de) | 2008-02-21 |
| JP2005537016A (ja) | 2005-12-08 |
| WO2004022745A2 (en) | 2004-03-18 |
| US8318497B2 (en) | 2012-11-27 |
| EP1556485B1 (de) | 2008-01-09 |
| EP1556485A2 (de) | 2005-07-27 |
| WO2004022745A3 (en) | 2005-06-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |