ATE383421T1 - Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentien - Google Patents
Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentienInfo
- Publication number
- ATE383421T1 ATE383421T1 AT03793509T AT03793509T ATE383421T1 AT E383421 T1 ATE383421 T1 AT E383421T1 AT 03793509 T AT03793509 T AT 03793509T AT 03793509 T AT03793509 T AT 03793509T AT E383421 T1 ATE383421 T1 AT E383421T1
- Authority
- AT
- Austria
- Prior art keywords
- reversible
- parallel
- cloning method
- multitask
- corresponding reagents
- Prior art date
Links
- 238000010367 cloning Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 230000002441 reversible effect Effects 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 230000002068 genetic effect Effects 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Electrotherapy Devices (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Plural Heterocyclic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40848202P | 2002-09-03 | 2002-09-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE383421T1 true ATE383421T1 (de) | 2008-01-15 |
Family
ID=31978622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03793509T ATE383421T1 (de) | 2002-09-03 | 2003-09-03 | Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentien |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8318497B2 (de) |
| EP (1) | EP1556485B1 (de) |
| JP (1) | JP4546828B2 (de) |
| CN (1) | CN1703509B (de) |
| AT (1) | ATE383421T1 (de) |
| AU (1) | AU2003260200A1 (de) |
| CA (1) | CA2496514C (de) |
| DE (1) | DE60318603T2 (de) |
| WO (1) | WO2004022745A2 (de) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT395483B (de) * | 1989-01-13 | 1993-01-25 | Vaillant Gmbh | Rein elektrisch beheizter durchlauferhitzer mit vorrichtung zum ermitteln des wasserdurchsatzes |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2180050B1 (de) | 1998-05-07 | 2016-04-20 | Universite Libre De Bruxelles | Auf Cytotoxinen basierendes biologisches Einschlusssystem |
| CA2435956C (en) | 2001-02-23 | 2012-07-10 | Universite Libre De Bruxelles | Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to toxic molecule |
| EP2119789A1 (de) | 2008-05-16 | 2009-11-18 | Université Libre de Bruxelles | Hyperproliferative rekombinante Zelle |
| EP2612918A1 (de) | 2012-01-06 | 2013-07-10 | BASF Plant Science Company GmbH | In planta-Rekombination |
| BR112016003533B1 (pt) | 2013-08-19 | 2021-11-03 | Syngulon Sa | Célula microbiana geneticamente modificada e método para controlar o crescimento de uma segunda célula microbiana em um meio de cultura |
| EP3164493B1 (de) * | 2014-07-04 | 2020-09-09 | Universite Libre De Bruxelles | Verfahren und system für die herstellung von rekombinanten proteinen durch zellen |
| CN106715681B (zh) * | 2014-07-25 | 2021-08-24 | R·P·谢勒科技有限责任公司 | 用于生产蛋白质的经改进的宿主细胞 |
| WO2019121983A1 (en) | 2017-12-19 | 2019-06-27 | Syngulon S.A. | Fermentation process |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE107697T1 (de) * | 1986-03-26 | 1994-07-15 | Genexpress Aps | Biologische eindämmung. |
| US5464764A (en) | 1989-08-22 | 1995-11-07 | University Of Utah Research Foundation | Positive-negative selection methods and vectors |
| US5300431A (en) * | 1991-02-26 | 1994-04-05 | E. I. Du Pont De Nemours And Company | Positive selection vector for the bacteriophage P1 cloning system |
| BE1006085A3 (fr) | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
| US7176029B2 (en) * | 1992-07-31 | 2007-02-13 | Universite Libre De Bruxelles | Cloning and/or sequencing vector |
| JP2590761B2 (ja) * | 1994-11-22 | 1997-03-12 | 日本電気株式会社 | Ｔａｂ式半導体装置およびｔａｂ式半導体装置を回路基板に接続する方法 |
| US6143557A (en) | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
| NZ500843A (en) * | 1995-06-07 | 2002-03-28 | Invitrogen Corp | A method of DNA recombination which is selectable to select for cells containing a product and against cells only harbouring the insert donor |
| NZ322673A (en) | 1995-10-13 | 1999-09-29 | Purdue Research Foundation | Method for the production of hybrid plants |
| US5922583A (en) * | 1995-10-17 | 1999-07-13 | Biostar Inc. | Methods for production of recombinant plasmids |
| JP4303418B2 (ja) | 1997-10-24 | 2009-07-29 | ライフ テクノロジーズ コーポレーション | 組換え部位を有する核酸を使用する組換えクローニング |
| EP2180050B1 (de) | 1998-05-07 | 2016-04-20 | Universite Libre De Bruxelles | Auf Cytotoxinen basierendes biologisches Einschlusssystem |
| US6134557A (en) * | 1998-11-20 | 2000-10-17 | Matlink, Inc. | Materials and supplies ordering system |
| US6271359B1 (en) * | 1999-04-14 | 2001-08-07 | Musc Foundation For Research Development | Tissue-specific and pathogen-specific toxic agents and ribozymes |
| NZ529476A (en) | 1999-10-25 | 2005-03-24 | Invitrogen Corp | Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination |
| CN1757724B (zh) | 1999-12-10 | 2014-06-11 | 茵维特罗根公司 | 具有独特特异性的多个重组位点在重组克隆中的用途 |
| EP1111061A1 (de) | 1999-12-20 | 2001-06-27 | Universite Libre De Bruxelles | Vektor mit doppelter Selektion |
| DE10038573A1 (de) | 2000-08-03 | 2002-02-21 | Mpb Cologne Gmbh Molecular Pla | Verfahren zur Selektion auf Wirtszellen mit eliminierten DNA-Sequenzen |
| CA2435956C (en) * | 2001-02-23 | 2012-07-10 | Universite Libre De Bruxelles | Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to toxic molecule |
| US6531153B2 (en) * | 2001-05-29 | 2003-03-11 | Drugtech Corporation | Composition with sustained release of levodopa and carbidopa |
| US20050260585A1 (en) * | 2002-03-19 | 2005-11-24 | Cedric Szpirer | Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms |
-
2003
- 2003-09-03 AU AU2003260200A patent/AU2003260200A1/en not_active Abandoned
- 2003-09-03 EP EP03793509A patent/EP1556485B1/de not_active Expired - Lifetime
- 2003-09-03 US US10/526,525 patent/US8318497B2/en not_active Expired - Fee Related
- 2003-09-03 CA CA2496514A patent/CA2496514C/en not_active Expired - Fee Related
- 2003-09-03 JP JP2004533086A patent/JP4546828B2/ja not_active Expired - Fee Related
- 2003-09-03 AT AT03793509T patent/ATE383421T1/de not_active IP Right Cessation
- 2003-09-03 WO PCT/BE2003/000147 patent/WO2004022745A2/en not_active Ceased
- 2003-09-03 DE DE60318603T patent/DE60318603T2/de not_active Expired - Lifetime
- 2003-09-03 CN CN038233053A patent/CN1703509B/zh not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT395483B (de) * | 1989-01-13 | 1993-01-25 | Vaillant Gmbh | Rein elektrisch beheizter durchlauferhitzer mit vorrichtung zum ermitteln des wasserdurchsatzes |
Also Published As
| Publication number | Publication date |
|---|---|
| DE60318603T2 (de) | 2008-07-03 |
| CA2496514A1 (en) | 2004-03-18 |
| US8318497B2 (en) | 2012-11-27 |
| WO2004022745A3 (en) | 2005-06-02 |
| EP1556485B1 (de) | 2008-01-09 |
| JP2005537016A (ja) | 2005-12-08 |
| US20060088841A1 (en) | 2006-04-27 |
| EP1556485A2 (de) | 2005-07-27 |
| CN1703509B (zh) | 2010-09-01 |
| CN1703509A (zh) | 2005-11-30 |
| AU2003260200A1 (en) | 2004-03-29 |
| DE60318603D1 (de) | 2008-02-21 |
| JP4546828B2 (ja) | 2010-09-22 |
| CA2496514C (en) | 2012-11-06 |
| WO2004022745A2 (en) | 2004-03-18 |
| AU2003260200A8 (en) | 2004-03-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |