ATE393237T1 - Mittel und verfahren zur in-vitro-bestimmung von camp - Google Patents

Mittel und verfahren zur in-vitro-bestimmung von camp

Info

Publication number
ATE393237T1
ATE393237T1 AT04819228T AT04819228T ATE393237T1 AT E393237 T1 ATE393237 T1 AT E393237T1 AT 04819228 T AT04819228 T AT 04819228T AT 04819228 T AT04819228 T AT 04819228T AT E393237 T1 ATE393237 T1 AT E393237T1
Authority
AT
Austria
Prior art keywords
camp
detectable labels
present
chimeric
camp binding
Prior art date
Application number
AT04819228T
Other languages
English (en)
Inventor
Viacheslav Nikolaev
Moritz Buenemann
Martin Lohse
Original Assignee
Bayerische Julius Maximilians
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayerische Julius Maximilians filed Critical Bayerische Julius Maximilians
Application granted granted Critical
Publication of ATE393237T1 publication Critical patent/ATE393237T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G01N33/5735—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes co-enzymes or co-factors, e.g. NAD or ATP
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT04819228T 2003-11-26 2004-11-26 Mittel und verfahren zur in-vitro-bestimmung von camp ATE393237T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03027311A EP1536020A1 (de) 2003-11-26 2003-11-26 Mittel und Verfahren zur optischen Bestimmung von cAMP in vitro und in vivo

Publications (1)

Publication Number Publication Date
ATE393237T1 true ATE393237T1 (de) 2008-05-15

Family

ID=34442896

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04819228T ATE393237T1 (de) 2003-11-26 2004-11-26 Mittel und verfahren zur in-vitro-bestimmung von camp

Country Status (8)

Country Link
US (1) US8889425B2 (de)
EP (2) EP1536020A1 (de)
JP (1) JP5296986B2 (de)
AT (1) ATE393237T1 (de)
CA (1) CA2547456C (de)
DE (1) DE602004013361T2 (de)
ES (1) ES2305906T3 (de)
WO (1) WO2005052186A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005038029A2 (en) 2003-10-10 2005-04-28 Promega Corporation Luciferase biosensor
EP1791972A4 (de) * 2004-08-23 2009-12-02 Univ Johns Hopkins cAMP-REPORTER
US8236523B2 (en) 2004-08-23 2012-08-07 The Johns Hopkins University Camp reporters and high throughput assays
WO2006103101A2 (en) 2005-03-31 2006-10-05 Julius-Maximilians-Universität Würzburg MEANS FOR THE INHIBITION OF ANTI-β1-ADRENERGIC RECEPTOR ANTIBODIES
FR2890174B1 (fr) * 2005-08-30 2009-04-24 Cis Bio Internat Sa Procede pour la mise en evidence d'un processus biologique par mesure d'un fret
WO2007100618A2 (en) * 2006-02-23 2007-09-07 Atto Bioscience Novel cell-based phosphodiesterase assays
US9359635B2 (en) 2006-04-03 2016-06-07 Promega Corporation Permuted and nonpermuted luciferase biosensors
AU2008291296B2 (en) 2007-08-24 2014-02-06 Julius-Maximilians-Universitat Wurzburg Mutant double cyclized receptor peptides inhibiting beta1-adrenoceptor antibodies
ATE542903T1 (de) 2008-05-19 2012-02-15 Promega Corp Luciferase-biosensoren für camp
EP2391637A1 (de) 2009-01-27 2011-12-07 Julius-Maximilians-Universität Würzburg Neue peptidhomologe zum inhibieren von beta1-adrenozeptor-antikörpern
JP5586285B2 (ja) * 2009-03-18 2014-09-10 オリンパス株式会社 受容体相互作用検出方法およびサイクリックａｍｐセンサータンパク質
US20120082976A1 (en) * 2009-04-17 2012-04-05 Kobe University Method of screening for insulin secretion-potentiating agents
DE102010018878B4 (de) 2009-04-30 2013-09-26 Julius-Maximilians-Universität Würzburg Neue Zell-Linie zur Fluoreszenz-basierten Detektion von funktionell aktiven Antikörpern und Autoantikörpern gegen den Beta1-adrenergen Rezeptor
US9290794B2 (en) 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
EP2990479B1 (de) 2010-05-11 2019-03-27 Promega Corporation Mutante protease-biosensoren mit verbesserten erkennungseigenschaften
EP3056902B1 (de) * 2015-02-16 2020-04-01 Universite De Bordeaux Neuartige spannungsabhängige Ionenkanalfusionen und Verwendungsverfahren dafür
DE102015115640B4 (de) * 2015-09-16 2017-06-29 Stiftung Caesar Center Of Advanced European Studies And Research Verfahren zum Nachweis von Liganden vermittels Biosensoren
JP6885595B2 (ja) * 2015-12-04 2021-06-16 国立大学法人 東京大学 リガンド蛍光センサータンパク質とその使用
CN108642064B (zh) * 2018-05-21 2021-11-26 安徽农业大学 小麦种子休眠持续期基因TaCNGC-2A及其功能标记
WO2020102721A1 (en) * 2018-11-16 2020-05-22 Rapa Therapeutics, Llc Immune monitoring of neuro-inflammatory amyotrophic lateral sclerosis (als)
KR101996767B1 (ko) * 2018-11-29 2019-07-04 씨제이제일제당 (주) cAMP 수용 단백질 변이체 및 이를 이용한 L-아미노산 제조방법
KR101991206B1 (ko) * 2018-11-29 2019-06-19 씨제이제일제당 (주) cAMP 수용 단백질 변이체 및 이를 이용한 L-아미노산 제조방법
KR101991207B1 (ko) * 2018-11-29 2019-06-19 씨제이제일제당 (주) cAMP 수용 단백질 변이체 및 이를 이용한 L-아미노산 제조방법
JP7613726B2 (ja) * 2021-02-09 2025-01-15 学校法人同志社 ペプチド及びｃＡＭＰ指示薬の製造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000049183A1 (en) * 1999-02-17 2000-08-24 Clontech Laboratories, Inc. FLUORESCENCE RESONANCE ENERGY TRANSFER DETECTION OF cAMP IN LIVING CELLS USING GFP VARIANTS
US6573059B1 (en) * 1999-06-04 2003-06-03 Rmf Dictagene S.A. Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements
EP1309624A2 (de) * 2000-07-03 2003-05-14 Smithkline Beecham Plc Hcn polypeptide und polynukleotide und deren verwendung in therapieverfahren
WO2002014373A1 (fr) 2000-08-14 2002-02-21 Michiyuki Matsuda Surveillance proteique de l'activite de la proteine de liaison gtp de bas poids moleculaire
AU2001232219A1 (en) 2000-08-14 2001-06-06 Michiyuki Matsuda Protein monitoring the activity of small gtp-binding protein

Also Published As

Publication number Publication date
US8889425B2 (en) 2014-11-18
EP1536020A1 (de) 2005-06-01
ES2305906T3 (es) 2008-11-01
CA2547456C (en) 2015-06-30
JP2007515160A (ja) 2007-06-14
CA2547456A1 (en) 2005-06-09
EP1687441B1 (de) 2008-04-23
DE602004013361T2 (de) 2009-05-20
US20080286760A1 (en) 2008-11-20
WO2005052186A1 (en) 2005-06-09
JP5296986B2 (ja) 2013-09-25
DE602004013361D1 (en) 2008-06-05
EP1687441A1 (de) 2006-08-09

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