ATE411396T1 - Methode zur bestimmung der effizienz von nukleinsäureamplifizierungen - Google Patents

Methode zur bestimmung der effizienz von nukleinsäureamplifizierungen

Info

Publication number
ATE411396T1
ATE411396T1 AT01107738T AT01107738T ATE411396T1 AT E411396 T1 ATE411396 T1 AT E411396T1 AT 01107738 T AT01107738 T AT 01107738T AT 01107738 T AT01107738 T AT 01107738T AT E411396 T1 ATE411396 T1 AT E411396T1
Authority
AT
Austria
Prior art keywords
nucleic acid
determining
efficiency
target nucleic
amplification
Prior art date
Application number
AT01107738T
Other languages
English (en)
Inventor
Karim Tabiti
Gregor Sagner
Richie Soong
Martin Gutekunst
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00107036A external-priority patent/EP1138780A1/de
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE411396T1 publication Critical patent/ATE411396T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT01107738T 2000-03-31 2001-04-02 Methode zur bestimmung der effizienz von nukleinsäureamplifizierungen ATE411396T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00107036A EP1138780A1 (de) 2000-03-31 2000-03-31 Detektion ausgesäter Tumorzellen mittels quantitativer RT-PCR auf Cytokeratin-20 mRNA
DE10034209 2000-07-13
DE10045521A DE10045521A1 (de) 2000-03-31 2000-09-13 Nukleinsäureamplifikationen

Publications (1)

Publication Number Publication Date
ATE411396T1 true ATE411396T1 (de) 2008-10-15

Family

ID=27213959

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01107738T ATE411396T1 (de) 2000-03-31 2001-04-02 Methode zur bestimmung der effizienz von nukleinsäureamplifizierungen

Country Status (6)

Country Link
US (2) US7125691B2 (de)
EP (1) EP1138784B1 (de)
JP (1) JP4022600B2 (de)
AT (1) ATE411396T1 (de)
DE (2) DE10045521A1 (de)
ES (1) ES2313919T3 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
DE69940647D1 (de) 1998-05-01 2009-05-07 Gen Probe Inc Automatische diagnostische Analysevorrichtung
US6691041B2 (en) 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10045521A1 (de) 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
ATE428925T1 (de) * 2001-11-20 2009-05-15 Exact Sciences Corp Automatische probenvorbereitungsverfahren und - vorrichtungen
NL1021160C2 (nl) * 2002-07-26 2004-01-27 Multigen Internat B V Werkwijze voor het bepalen van het kopieaantal van een nucleotidevolgorde.
US7788039B2 (en) * 2003-09-25 2010-08-31 Roche Molecular Systems, Inc. Quantitation of nucleic acids using growth curves
ATE496141T1 (de) 2003-12-06 2011-02-15 Abbott Lab Verfahren und system zum analysieren von reaktionen unter verwendung eines informationssystems
JP2006042803A (ja) * 2004-06-28 2006-02-16 Sumitomo Chemical Co Ltd コモンマーモセット由来のシクロフィリンａ遺伝子及びその利用
WO2006085964A2 (en) * 2004-06-30 2006-08-17 Applera Corporation Log-linear amplification
JP4777631B2 (ja) 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ 核酸増幅分析法および装置
WO2006076396A2 (en) 2005-01-13 2006-07-20 Hsbc North America Holdings Inc. Framework for configuration and release management of group system software
DE102005006635A1 (de) 2005-01-31 2006-08-10 Osram Opto Semiconductors Gmbh Optisches Element und Verfahren zu dessen Herstellung
US7794659B2 (en) 2005-03-10 2010-09-14 Gen-Probe Incorporated Signal measuring system having a movable signal measuring device
EP1921454B1 (de) * 2005-03-10 2015-08-12 Gen-Probe Incorporated Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten
CA2627259C (en) 2005-11-14 2016-02-23 Gen-Probe Incorporated Parametric calibration method
AU2007325037B2 (en) * 2006-11-30 2012-09-20 Gen-Probe Incorporated Quantitative method employing adjustment of pre-defined master calibration curves
AU2008240044B2 (en) * 2007-04-13 2013-09-12 Teva Pharamceuticals International Gmbh Oral cephalotaxine dosage forms
EP2220227B1 (de) * 2007-07-13 2014-09-10 The United States of America, as represented by The Secretary of the Army, on behalf of the U.S. Army Med. Research Institute of Chem. Defense Einmalige kalibrator-polynukleotide und verfahren zur verwendung bei quantitativen nukleinsäure-tests
KR101413659B1 (ko) * 2007-12-06 2014-07-01 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 시료 중의 표적 핵산의초기 농도를 결정하는 방법
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
BRPI1006682B1 (pt) 2009-04-16 2020-04-22 Koninl Philips Electronics Nv método para a quantificação relativa de no mínimo um ácido nucleico alvo em uma amostra, método para a quantificação absoluta de no mínimo um ácido nucleico alvo em uma amostra, meios legíveis por máquina e equipamento para a análise das amostras de ácido nucleico
JP5575239B2 (ja) 2009-07-21 2014-08-20 ジェン−プローブ・インコーポレーテッド 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物
WO2011017567A1 (en) 2009-08-05 2011-02-10 Life Technologies Corporation Methods for the analysis of proximity binding assay data
JP5848750B2 (ja) * 2010-04-11 2016-01-27 ライフ テクノロジーズ コーポレーション モデルベースのｑＰＣＲのためのシステムおよび方法
KR20130029128A (ko) 2010-07-23 2013-03-21 베크만 컬터, 인코포레이티드 실시간 pcr용 용기
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
EP2437192A1 (de) 2010-09-16 2012-04-04 Roche Diagnostics GmbH Relative Quantifizierungsanalyse für mehrparametrische PCR-Experimente
US20130304390A1 (en) * 2010-11-16 2013-11-14 Life Technologies Corporation Systems and Methods for the Analysis of Proximity Binding Assay Data
EP2678664B1 (de) 2011-02-24 2019-08-07 Gen-Probe Incorporated Systeme und verfahren zur unterscheidung optischer signale mit verschiedenen modulationsfrequenzen bei einem optischen signaldetektor
EP3373015A1 (de) 2011-11-07 2018-09-12 Beckman Coulter Inc. Aliquotierungssystem und arbeitsablauf
US8973736B2 (en) 2011-11-07 2015-03-10 Beckman Coulter, Inc. Magnetic damping for specimen transport system
KR20140091033A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 컨테이너 검출
WO2013070755A2 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Centrifuge system and workflow
US9910054B2 (en) 2011-11-07 2018-03-06 Beckman Coulter, Inc. System and method for processing samples
CN104040352B (zh) 2011-11-07 2016-09-07 贝克曼考尔特公司 机械臂
ITMO20120015A1 (it) * 2012-01-26 2013-07-27 Guescini Michele Proprieta Al 25 Metodo di perfezionamento per la determinazione quantitativa degli acidi nucleici
US20150267260A1 (en) 2012-05-25 2015-09-24 Accugenomics, Inc. Nucleic acid amplification and use thereof
WO2014018885A2 (en) 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
US9957557B2 (en) 2012-08-13 2018-05-01 Life Genetics Lab, Llc Development of a highly sensitive quantification system for assessing DNA degradation and quality in forensic samples
US10993418B2 (en) 2012-08-13 2021-05-04 Life Genetics Lab, Llc Method for measuring tumor burden in patient derived xenograft (PDX) mice
EP2971154A4 (de) * 2013-03-14 2017-03-01 Ibis Biosciences, Inc. Nukleinsäurekontrollpanels
US9976175B2 (en) 2013-03-15 2018-05-22 Gen-Probe Incorporated Calibration method, apparatus and computer program product
EP3765639A4 (de) * 2018-03-16 2021-12-22 Spartan Bioscience Inc. Linear amplifizierte interne kontrolle für nukleinsäureamplifikationsreaktion
CN111816256B (zh) * 2020-01-07 2024-03-29 江苏普瑞悉恩生物科技有限公司 核酸样本检测方法及装置、存储介质及电子设备
DE102020202358B4 (de) * 2020-02-25 2023-09-21 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Durchführung eines qPCR-Verfahrens

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2516655A (en) * 1945-08-30 1950-07-25 Maytag Co Oscillating washing machine tub
US3163404A (en) * 1962-10-09 1964-12-29 Scientific Industries Rotary apparatus for agitating fluids
US3614434A (en) * 1968-07-17 1971-10-19 Lofstrom James E Automatic agitating and sample device
US3711379A (en) * 1971-06-24 1973-01-16 Cenco Medical Health Supply Co Rotating flask culture apparatus
GB9020352D0 (en) * 1990-09-18 1990-10-31 Anagen Ltd Assay or reaction apparatus
CA2129787A1 (en) 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
US5499872A (en) * 1994-03-14 1996-03-19 Baxter; Michael Turntable mixer apparatus
AT401270B (de) * 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
DE69739357D1 (de) * 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
EP1442794B1 (de) * 1996-06-04 2006-03-29 University Of Utah Research Foundation Vorrichtung und Verfahren zur Durchführung und Überprüfung von biologischen Prozessen
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
KR20010034814A (ko) 1998-04-23 2001-04-25 제넨테크, 인크. 유전자 발현의 정량적 분석
US6066458A (en) 1998-05-18 2000-05-23 Becton, Dickinson And Company Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates
EP1107738A4 (de) 1998-08-27 2003-01-22 Bristol Myers Squibb Co Neuartige pharmazeitische salzform
GB9819272D0 (en) 1998-09-03 1998-10-28 Andaris Ltd Microparticles
US6235504B1 (en) * 1999-01-11 2001-05-22 The Rockefeller University Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein
DE10045521A1 (de) 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
US6691041B2 (en) * 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids

Also Published As

Publication number Publication date
US20020058262A1 (en) 2002-05-16
ES2313919T3 (es) 2009-03-16
JP4022600B2 (ja) 2007-12-19
JP2001314194A (ja) 2001-11-13
US20050009048A1 (en) 2005-01-13
EP1138784B1 (de) 2008-10-15
EP1138784A2 (de) 2001-10-04
DE10045521A1 (de) 2001-10-04
DE60136121D1 (de) 2008-11-27
EP1138784A3 (de) 2003-05-02
US7378241B2 (en) 2008-05-27
US7125691B2 (en) 2006-10-24

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