ATE411396T1 - Methode zur bestimmung der effizienz von nukleinsäureamplifizierungen - Google Patents

Methode zur bestimmung der effizienz von nukleinsäureamplifizierungen

Info

Publication number
ATE411396T1
ATE411396T1 AT01107738T AT01107738T ATE411396T1 AT E411396 T1 ATE411396 T1 AT E411396T1 AT 01107738 T AT01107738 T AT 01107738T AT 01107738 T AT01107738 T AT 01107738T AT E411396 T1 ATE411396 T1 AT E411396T1
Authority
AT
Austria
Prior art keywords
nucleic acid
determining
efficiency
target nucleic
amplification
Prior art date
Application number
AT01107738T
Other languages
English (en)
Inventor
Karim Tabiti
Gregor Sagner
Richie Soong
Martin Gutekunst
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00107036A external-priority patent/EP1138780A1/de
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE411396T1 publication Critical patent/ATE411396T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT01107738T 2000-03-31 2001-04-02 Methode zur bestimmung der effizienz von nukleinsäureamplifizierungen ATE411396T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00107036A EP1138780A1 (de) 2000-03-31 2000-03-31 Detektion ausgesäter Tumorzellen mittels quantitativer RT-PCR auf Cytokeratin-20 mRNA
DE10034209 2000-07-13
DE10045521A DE10045521A1 (de) 2000-03-31 2000-09-13 Nukleinsäureamplifikationen

Publications (1)

Publication Number Publication Date
ATE411396T1 true ATE411396T1 (de) 2008-10-15

Family

ID=27213959

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01107738T ATE411396T1 (de) 2000-03-31 2001-04-02 Methode zur bestimmung der effizienz von nukleinsäureamplifizierungen

Country Status (6)

Country Link
US (2) US7125691B2 (de)
EP (1) EP1138784B1 (de)
JP (1) JP4022600B2 (de)
AT (1) ATE411396T1 (de)
DE (2) DE10045521A1 (de)
ES (1) ES2313919T3 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
DE10011529T1 (de) 1998-05-01 2011-09-01 Gen-Probe Incorporated Automatisches Diagnoseanalysegerät und Verfahren
DE10045521A1 (de) * 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
US6691041B2 (en) 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
WO2003044217A2 (en) * 2001-11-20 2003-05-30 Exact Sciences Corporation Automated sample preparation methods and devices
NL1021160C2 (nl) * 2002-07-26 2004-01-27 Multigen Internat B V Werkwijze voor het bepalen van het kopieaantal van een nucleotidevolgorde.
US7788039B2 (en) 2003-09-25 2010-08-31 Roche Molecular Systems, Inc. Quantitation of nucleic acids using growth curves
EP1706836B1 (de) 2003-12-06 2011-01-19 Abbott Laboratories Verfahren und system zum analysieren von reaktionen unter verwendung eines informationssystems
JP2006042803A (ja) * 2004-06-28 2006-02-16 Sumitomo Chemical Co Ltd コモンマーモセット由来のシクロフィリンａ遺伝子及びその利用
US20060057611A1 (en) * 2004-06-30 2006-03-16 Applera Corporation Log-linear amplification
JP4777631B2 (ja) 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ 核酸増幅分析法および装置
US7937685B2 (en) 2005-01-13 2011-05-03 Hsbc Technology & Services (Usa) Inc. Computer software implemented framework for configuration and release management of group systems software, and method for same
DE102005006635A1 (de) 2005-01-31 2006-08-10 Osram Opto Semiconductors Gmbh Optisches Element und Verfahren zu dessen Herstellung
US7964413B2 (en) 2005-03-10 2011-06-21 Gen-Probe Incorporated Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay
EP2348320B1 (de) * 2005-03-10 2024-05-01 Gen-Probe Incorporated Verfahren und Systeme zur Erkennung mehrerer Fluoreszenzemissionssignale
CA2627259C (en) 2005-11-14 2016-02-23 Gen-Probe Incorporated Parametric calibration method
EP2102773B1 (de) * 2006-11-30 2012-08-01 Gen-Probe Incorporated Quantitatives verfahren mit anpassung vordefinierter master-kalbrierkurven
AU2008240044B2 (en) * 2007-04-13 2013-09-12 Teva Pharamceuticals International Gmbh Oral cephalotaxine dosage forms
US8642746B2 (en) 2007-07-13 2014-02-04 The United States Of America As Represented By The Secretary Of The Army Unique calibrator polynucleotides and methods of using in quantitative nucleic acid assays
KR101413659B1 (ko) * 2007-12-06 2014-07-01 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 시료 중의 표적 핵산의초기 농도를 결정하는 방법
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
JP5810078B2 (ja) 2009-04-16 2015-11-11 コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． 核酸定量方法
CA2768391C (en) 2009-07-21 2016-09-06 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
EP2462515A4 (de) * 2009-08-05 2015-08-12 Life Technologies Corp Verfahren zur analyse von näherungsbindenden assay-daten
EP2558971B1 (de) * 2010-04-11 2021-02-17 Life Technologies Corporation Systeme und verfahren für qpcr auf modellbasis
KR20130029128A (ko) 2010-07-23 2013-03-21 베크만 컬터, 인코포레이티드 실시간 pcr용 용기
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
EP2437192A1 (de) 2010-09-16 2012-04-04 Roche Diagnostics GmbH Relative Quantifizierungsanalyse für mehrparametrische PCR-Experimente
US20130304390A1 (en) * 2010-11-16 2013-11-14 Life Technologies Corporation Systems and Methods for the Analysis of Proximity Binding Assay Data
EP2678664B1 (de) 2011-02-24 2019-08-07 Gen-Probe Incorporated Systeme und verfahren zur unterscheidung optischer signale mit verschiedenen modulationsfrequenzen bei einem optischen signaldetektor
JP6062449B2 (ja) 2011-11-07 2017-01-18 ベックマン コールター， インコーポレイテッド 標本コンテナ検出
BR112014011044A2 (pt) 2011-11-07 2017-04-25 Beckman Coulter Inc amortecimento magnético para sistema de transporte de espécime
BR112014010955A2 (pt) 2011-11-07 2017-06-06 Beckman Coulter Inc sistema e método para processar amostras
KR102040996B1 (ko) 2011-11-07 2019-11-05 베크만 컬터, 인코포레이티드 로봇식 아암
KR20140092375A (ko) 2011-11-07 2014-07-23 베크만 컬터, 인코포레이티드 원심분리기 시스템 및 작업 흐름
EP2776846B1 (de) 2011-11-07 2019-08-21 Beckman Coulter, Inc. Aliquotierungssystem und arbeitsablauf
ITMO20120015A1 (it) * 2012-01-26 2013-07-27 Guescini Michele Proprieta Al 25 Metodo di perfezionamento per la determinazione quantitativa degli acidi nucleici
EP2855704A4 (de) 2012-05-25 2016-03-16 Accugenomics Inc Nukleinsäureamplifikation und anwendung davon
WO2014018885A2 (en) 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
US9957557B2 (en) 2012-08-13 2018-05-01 Life Genetics Lab, Llc Development of a highly sensitive quantification system for assessing DNA degradation and quality in forensic samples
US10993418B2 (en) 2012-08-13 2021-05-04 Life Genetics Lab, Llc Method for measuring tumor burden in patient derived xenograft (PDX) mice
WO2014152937A1 (en) * 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
US9976175B2 (en) 2013-03-15 2018-05-22 Gen-Probe Incorporated Calibration method, apparatus and computer program product
EP3765639A4 (de) * 2018-03-16 2021-12-22 Spartan Bioscience Inc. Linear amplifizierte interne kontrolle für nukleinsäureamplifikationsreaktion
CN111816256B (zh) * 2020-01-07 2024-03-29 江苏普瑞悉恩生物科技有限公司 核酸样本检测方法及装置、存储介质及电子设备
DE102020202358B4 (de) 2020-02-25 2023-09-21 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Durchführung eines qPCR-Verfahrens

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2516655A (en) 1945-08-30 1950-07-25 Maytag Co Oscillating washing machine tub
US3163404A (en) 1962-10-09 1964-12-29 Scientific Industries Rotary apparatus for agitating fluids
US3614434A (en) 1968-07-17 1971-10-19 Lofstrom James E Automatic agitating and sample device
US3711379A (en) 1971-06-24 1973-01-16 Cenco Medical Health Supply Co Rotating flask culture apparatus
GB9020352D0 (en) 1990-09-18 1990-10-31 Anagen Ltd Assay or reaction apparatus
CA2129787A1 (en) 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
US5499872A (en) 1994-03-14 1996-03-19 Baxter; Michael Turntable mixer apparatus
AT401270B (de) * 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
JP4281877B2 (ja) * 1996-06-04 2009-06-17 ユニバーシティ オブ ユタ リサーチ ファウンデーション 生物学的プロセスを実行し且つモニタリングするためのシステムと方法
EP1033411B1 (de) * 1996-06-04 2006-02-22 University of Utah Research Foundation Fluoreszenz-Donor-Akzeptor Paar
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
KR20010034814A (ko) 1998-04-23 2001-04-25 제넨테크, 인크. 유전자 발현의 정량적 분석
US6066458A (en) 1998-05-18 2000-05-23 Becton, Dickinson And Company Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates
CA2341522A1 (en) 1998-08-27 2000-03-09 Bristol-Myers Squibb Company Novel pharmaceutical salt form
GB9819272D0 (en) 1998-09-03 1998-10-28 Andaris Ltd Microparticles
US6235504B1 (en) * 1999-01-11 2001-05-22 The Rockefeller University Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein
US6691041B2 (en) * 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10045521A1 (de) 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen

Also Published As

Publication number Publication date
EP1138784B1 (de) 2008-10-15
US7125691B2 (en) 2006-10-24
DE60136121D1 (de) 2008-11-27
JP4022600B2 (ja) 2007-12-19
EP1138784A2 (de) 2001-10-04
JP2001314194A (ja) 2001-11-13
DE10045521A1 (de) 2001-10-04
US20020058262A1 (en) 2002-05-16
EP1138784A3 (de) 2003-05-02
US7378241B2 (en) 2008-05-27
US20050009048A1 (en) 2005-01-13
ES2313919T3 (es) 2009-03-16

Similar Documents

Publication Publication Date Title
DE60136121D1 (de) Methode zur Bestimmung der Effizienz von Nukleinsäureamplifizierungen
DK0497784T3 (da) Kvantificering af nukleinsyrer under anvendelse af polymerasekædereaktionen
DE60129869D1 (de) Methode zur Quantifizierung von Nukleinsäuren in Echtzeit mittels Effizienzkorrektur
ATA183094A (de) Verfahren zur quantifizierung von genomischer dna
ES2082217T3 (es) Procedimiento de amplificacion de la señal de emision de un compuesto luminiscente.
EA200400329A1 (ru) Способы амплификации нуклеиновых кислот
DE60023056D1 (de) Verfahren zur Quantifizierung eines Analyten
DE60035459D1 (de) Automatisierte Echtzeit-Analyse von Nukleinsäureamplifikation
EA200300284A1 (ru) Способ амплификации нуклеиновых кислот
ATE394506T1 (de) Verfahren zur bestimmung von nukleinsäuren mittels einer kontrolle
WO2004022721A3 (en) Quantification of gene expression
WO2007024840A3 (en) Method of quantitating nucleic acids by flow cytometry microparticle-based array
WO2001084463A3 (en) Apparatus, method, and computer program product for quantitative analysis of a nucleic acid amplification reaction
ATE304061T1 (de) Polynukleotid amplifizierungsverfahren
DE602004005711D1 (de) Schnell-verfahren zur detektion von mikroorganismen in lebensmittelproben
ATE230801T1 (de) Verfahren zur detektion von zielnukleinsäure, verfahren zu ihrer quantifizierung und pyrylium- verbindungen zur chemilumineszenz-analyse
EP1055736A4 (de) Verfaheren zur dna-sequenzierung
DK0714988T3 (da) Fremgangsmåde til mængdebestemmelse af nukleinsyrer
ATE372392T1 (de) Verfahren zur bestimmung der menge einer zielnukleinsäure in einer probe
DE59609841D1 (de) Verfahren zur Gattungs- und speziesspezifische Identifizierung von Legionellen
DE60132924D1 (de) Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2)
ES2152995T3 (es) Deteccion de la amplificacion de acido nucleico mediante polarizacion por fluorescencia.
CA2360012A1 (en) Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
WO2002101091A3 (fr) Procede de detection et d'identification de la presence de matieres biologiques provenant de poissons, et oligonucleotides pour sa mise en oeuvre
DE60331132D1 (de) Verfahren zur Quantifizierung von Nukleinsäuren

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1138784

Country of ref document: EP