ATE366821T1 - Automatisierte echtzeit-analyse von nukleinsäureamplifikation - Google Patents

Automatisierte echtzeit-analyse von nukleinsäureamplifikation

Info

Publication number
ATE366821T1
ATE366821T1 AT00303519T AT00303519T ATE366821T1 AT E366821 T1 ATE366821 T1 AT E366821T1 AT 00303519 T AT00303519 T AT 00303519T AT 00303519 T AT00303519 T AT 00303519T AT E366821 T1 ATE366821 T1 AT E366821T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid amplification
time analysis
automated real
sample
Prior art date
Application number
AT00303519T
Other languages
English (en)
Inventor
Carl T Wittwer
Original Assignee
Univ Utah Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Utah Res Found filed Critical Univ Utah Res Found
Application granted granted Critical
Publication of ATE366821T1 publication Critical patent/ATE366821T1/de

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Optics & Photonics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
AT00303519T 1999-04-27 2000-04-26 Automatisierte echtzeit-analyse von nukleinsäureamplifikation ATE366821T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13125699P 1999-04-27 1999-04-27
US09/391,811 US6387621B1 (en) 1999-04-27 1999-09-08 Automated analysis of real-time nucleic acid amplification

Publications (1)

Publication Number Publication Date
ATE366821T1 true ATE366821T1 (de) 2007-08-15

Family

ID=26829286

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00303519T ATE366821T1 (de) 1999-04-27 2000-04-26 Automatisierte echtzeit-analyse von nukleinsäureamplifikation

Country Status (5)

Country Link
US (2) US6387621B1 (de)
EP (1) EP1059523B1 (de)
JP (1) JP4689004B2 (de)
AT (1) ATE366821T1 (de)
DE (1) DE60035459T2 (de)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
WO2001073127A2 (en) * 2000-03-27 2001-10-04 Applera Corporation Improved invasion assay
ATE408710T1 (de) * 2001-05-07 2008-10-15 Mayo Foundation Nachweis von legionella durch ein pcr und fret verwendendes verfahren
US6979541B1 (en) 2001-07-26 2005-12-27 University Of Utah Research Foundation Methods for identifying chromosomal aneuploidy
US7188030B2 (en) * 2001-08-21 2007-03-06 Applera Corporation Automatic threshold setting for quantitative polymerase chain reaction
ES2269614T3 (es) * 2001-08-31 2007-04-01 The University Of Utah Research Foundation Cuantificacion de genes en tiempo real con estandares internos.
US7373253B2 (en) * 2002-02-12 2008-05-13 Idaho Technology Multi-test analysis of real-time nucleic acid amplification
ATE384139T1 (de) * 2002-02-12 2008-02-15 Univ Utah Res Found Multi-test-analyse von echzeit- nukleinsäureamplifikationen
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
CA2477761A1 (en) 2002-03-01 2003-09-12 Ravgen, Inc. Methods for detection of genetic disorders
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
AUPS267802A0 (en) * 2002-05-30 2002-06-20 Bio-Molecular Holdings Pty Limited Improved dna amplification apparatus and method
US7083974B2 (en) * 2002-07-12 2006-08-01 Applera Corporation Rotatable sample disk and method of loading a sample disk
AU2003275138A1 (en) * 2002-09-19 2004-04-08 Applied Biosystems, Llc. Methods and compositions for detecting targets
US7074598B2 (en) * 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
CA2506151C (en) * 2002-11-15 2010-08-03 Gen-Probe Incorporated Assay and compositions for detection of bacillus anthracis nucleic acid
MXPA05009140A (es) * 2003-02-28 2006-04-28 Ravgen Inc Metodo para la deteccion de trastornos geneticos.
EP2390352A1 (de) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systeme und Methoden zur Erhöhung der Milchproduktion bei Tieren
US20040185446A1 (en) * 2003-03-18 2004-09-23 Jones Alison M. Cpn60 targets for quantification of microbial species
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
EP1718770B1 (de) 2004-02-19 2011-05-25 The Governors of the University of Alberta Leptinpromotor-polymorphismen und verwendungen davon
KR100738073B1 (ko) 2004-09-01 2007-07-12 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 초기 핵산 농도를정량화하는 방법
US20060246423A1 (en) * 2005-02-10 2006-11-02 Adelson Martin E Method and kit for the collection and maintenance of the detectability of a plurality of microbiological species in a single gynecological sample
US20060178838A1 (en) * 2005-02-10 2006-08-10 Adelson Martin E Method of receiving and handling a plurality of clinical samples for reporting a sum of diagnostic results for each sample
US9464310B2 (en) * 2005-02-10 2016-10-11 Medical Diagnostic Laboratories, Llc Integrated method for collection and maintenance of detectability of a plurality of microbiological agents in a single clinical sample and for handling a plurality of samples for reporting a sum of diagnostic results for each sample
WO2006099255A2 (en) 2005-03-10 2006-09-21 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
EP1897016B1 (de) * 2005-06-22 2012-08-01 Gen-Probe Incorporated Verfahren und algorithmus zum quantifizieren von polynukleotiden
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
EP1798542A1 (de) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytisches Verfahren und Gerät
RU2294532C1 (ru) * 2006-02-14 2007-02-27 Закрытое акционерное общество "Научно-производственная фирма ДНК-Технология" Способ стандартизации данных полимерной цепной реакции с регистрацией накопления продуктов реакции по флуоресценции непосредственно во время реакции (пцр "в реальном времени")
US20070238093A1 (en) * 2006-04-11 2007-10-11 Espy Mark J Detection of influenza A virus
US20070238095A1 (en) * 2006-04-11 2007-10-11 Mayo Foundation For Medical Education And Research , A Minnesota Corporation Detection of Influenza A Virus
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック, インコーポレイテッド レコンビナーゼポリメラーゼ増幅
JP4993260B2 (ja) * 2006-05-15 2012-08-08 石川県 遺伝子定量装置及び定量方法
US7802215B2 (en) * 2006-06-06 2010-09-21 Fujitsu Limited System and method for providing an improved sliding window scheme for clock mesh analysis
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US20080182263A1 (en) * 2007-01-29 2008-07-31 Applera Corporation Systems and Methods for Calibration Using Dye Signal Amplification
CN101688841B (zh) * 2007-06-29 2011-12-07 霍夫曼-拉罗奇有限公司 用于确定pcr和其他数据集中的串扰系数的系统和方法
US20090055243A1 (en) 2007-08-21 2009-02-26 Jayson Lee Lusk Systems and methods for predicting a livestock marketing method
US8386184B2 (en) * 2007-08-28 2013-02-26 Becton, Dickinson And Company Systems and methods for determining an amount of starting reagent using the polymerase chain reaction
US8380457B2 (en) * 2007-08-29 2013-02-19 Canon U.S. Life Sciences, Inc. Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes
EP2215265B1 (de) * 2007-11-14 2013-05-22 Grifols Therapeutics Inc. Verfahren zur anpassung der ergebnisse eines polymerasekettenreaktionsinstruments
KR101413659B1 (ko) 2007-12-06 2014-07-01 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 시료 중의 표적 핵산의초기 농도를 결정하는 방법
JP5542439B2 (ja) * 2007-12-26 2014-07-09 アークレイ株式会社 核酸増幅判定方法および核酸増幅判定装置
US20100055733A1 (en) * 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
EP2166470A1 (de) * 2008-09-19 2010-03-24 Corbett Research Pty Ltd Analyse von Schmelzkurven, insbesondere dsDNA- und Proteinschmelzkurven
US20100119454A1 (en) * 2008-11-03 2010-05-13 Ping Shen Use of the conserved Drosophila NPFR1 system for uncovering interacting genes and pathways important in nociception and stress response
GB0905325D0 (en) 2009-03-30 2009-05-13 Selex Sensors & Airborne Sys Detection system
JP5847076B2 (ja) * 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Dnaグリコシラーゼ/リアーゼおよびapエンドヌクレアーゼ基質
EP3360974B1 (de) 2009-06-05 2025-04-09 Abbott Diagnostics Scarborough, Inc. Reagentien zur rekombinase polymerase amplifikation
EP2462515A4 (de) 2009-08-05 2015-08-12 Life Technologies Corp Verfahren zur analyse von näherungsbindenden assay-daten
US8023794B2 (en) * 2009-08-10 2011-09-20 The Boeing Company Apparatus and method for establishing an optical path spanning a discontinuity in an optical channel
ES2743959T3 (es) 2009-10-21 2020-02-21 Univ Brandeis Kits para analizar secuencias de ácido nucleico monocatenario
RU2423685C1 (ru) * 2010-05-20 2011-07-10 Александр Иванович Заико Способ стандартизации данных конвекционной полимерной цепной реакции с регистрацией накопления продуктов реакций по флуоресценции непосредственно во время реакции
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
CN103403533B (zh) 2011-02-24 2017-02-15 简.探针公司 用于分辨光信号检测器中不同调制频率的光信号的系统和方法
US8809021B2 (en) 2011-04-07 2014-08-19 Alere San Diego Inc. Monitoring recombinase polymerase amplification mixtures
CA2849023C (en) 2011-09-15 2022-07-19 David A. Shafer Probe:antiprobe compositions for high specificity dna or rna detection
AU2013203281A1 (en) 2012-02-03 2013-08-22 Axxin Pty Ltd Nucleic acid amplification and detection apparatus and method
AU2015275310B2 (en) * 2012-02-03 2018-10-18 Axxin Pty Ltd Nucleic acid amplification and detection apparatus and method
US20130273547A1 (en) * 2012-04-16 2013-10-17 Samsung Techwin Co., Ltd. Method to determine and correct baseline and to characterize pcr amplification kinetics
US9932629B2 (en) 2012-06-26 2018-04-03 Axxin Pty Ltd Nucleic acid amplification and detection kit
WO2014018885A2 (en) 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
SG11201501550QA (en) 2012-09-10 2015-03-30 Biofire Diagnostics Llc Multiple amplification cycle detection
US9714447B2 (en) 2013-08-19 2017-07-25 General Electric Company Detection of nucleic acid amplification in a porous substrate
EP3218698B1 (de) 2014-11-14 2020-05-13 Axxin Pty Ltd Anordnung zur sammlung und aufbewahrung biologischer proben
WO2017011862A1 (en) 2015-07-17 2017-01-26 Axxin Pty Ltd Diagnostic test assembly, apparatus, method
EP3600403A4 (de) 2017-03-24 2021-01-13 Board of Supervisors of Louisiana State University and Agricultural and Mechanical College Impfstoffstamm vc2 gegen herpes-simplex-virus typ-1 (hsv-1) zur erzeugung einer anti-ehv-1-immunantwort
US11398296B2 (en) 2017-07-21 2022-07-26 Biofire Diagnostics, Llc Detection using concurrent melting curves
AU2018340855B2 (en) 2017-09-27 2023-06-22 Axxin Pty Ltd Diagnostic test system and method
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
EP3895170B1 (de) * 2018-12-14 2025-10-29 Seegene, Inc. Verfahren zum nachweis eines zielanalyten in einer probe unter verwendung einer s-förmigen funktion für einen anstiegsdatensatz
ES2969861T3 (es) 2019-12-20 2024-05-23 Euroimmun Medizinische Labordiagnostika Ag Procedimiento y dispositivo para la evaluación cualitativa de datos de PCR en tiempo real
CN111426661B (zh) * 2020-04-09 2022-10-18 基蛋生物科技股份有限公司 一种核酸扩增阶段的荧光数据采集及判别方法
KR102407584B1 (ko) * 2020-08-21 2022-06-10 한림대학교 산학협력단 인공지능을 이용한 중합효소 연쇄반응 양성 판단을 위한 검사 방법
KR102407585B1 (ko) * 2020-08-21 2022-06-10 한림대학교 산학협력단 인공지능을 이용한 중합효소 연쇄반응 음성 판단을 위한 검사 방법
CN112816446B (zh) * 2020-12-24 2022-02-01 四川长虹电器股份有限公司 一种基于荧光光谱检测荧光轮粉体衰变的方法
CN112669910A (zh) * 2020-12-30 2021-04-16 杭州博日科技股份有限公司 扩增曲线基线确定方法、装置以及电子设备
EP4359769A4 (de) * 2021-08-25 2024-07-24 Hewlett-Packard Development Company, L.P. Nukleinsäurestrangdetektionen
JP2025540566A (ja) 2022-11-22 2025-12-16 ビオメリュー 新規核酸結合化合物及び使用
WO2025141105A1 (en) 2023-12-27 2025-07-03 bioMérieux Specific probes for the detection of nucleic acids, methods and uses
WO2025257150A1 (en) 2024-06-11 2025-12-18 Roche Diagnostics International Ag Computer-implemented method for early-stage prediction of pcr result

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4592365A (en) * 1981-08-10 1986-06-03 Ivac Corporation Electronic sphygmomanometer
US5455175A (en) 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
WO1997046707A2 (en) 1996-06-04 1997-12-11 University Of Utah Research Foundation System and method for monitoring for dna amplification by fluorescence
US6174670B1 (en) * 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
US6303305B1 (en) * 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification

Also Published As

Publication number Publication date
DE60035459T2 (de) 2008-03-20
JP2000333700A (ja) 2000-12-05
EP1059523A3 (de) 2003-05-21
US20020123062A1 (en) 2002-09-05
US6387621B1 (en) 2002-05-14
JP4689004B2 (ja) 2011-05-25
DE60035459D1 (de) 2007-08-23
EP1059523A2 (de) 2000-12-13
EP1059523B1 (de) 2007-07-11
US20020042051A1 (en) 2002-04-11

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