ATE412761T1 - Methode um rekombinante proteine in cho zellen zu exprimieren - Google Patents
Methode um rekombinante proteine in cho zellen zu exprimierenInfo
- Publication number
- ATE412761T1 ATE412761T1 AT03765075T AT03765075T ATE412761T1 AT E412761 T1 ATE412761 T1 AT E412761T1 AT 03765075 T AT03765075 T AT 03765075T AT 03765075 T AT03765075 T AT 03765075T AT E412761 T1 ATE412761 T1 AT E412761T1
- Authority
- AT
- Austria
- Prior art keywords
- cho cells
- recombinant proteins
- express recombinant
- express
- enhancing
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 238000001890 transfection Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0216648.6A GB0216648D0 (en) | 2002-07-18 | 2002-07-18 | Method of expressing recombinant protein in CHO cells |
| PCT/EP2003/007946 WO2004009823A1 (en) | 2002-07-18 | 2003-07-21 | Method of expressing recombinant protein in cho cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE412761T1 true ATE412761T1 (de) | 2008-11-15 |
Family
ID=9940670
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03765075T ATE412761T1 (de) | 2002-07-18 | 2003-07-21 | Methode um rekombinante proteine in cho zellen zu exprimieren |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US20060003405A1 (de) |
| EP (1) | EP1525320B1 (de) |
| CN (1) | CN1296486C (de) |
| AT (1) | ATE412761T1 (de) |
| AU (2) | AU2003251434B2 (de) |
| CA (2) | CA2489016C (de) |
| DE (1) | DE60324430D1 (de) |
| DK (1) | DK1525320T3 (de) |
| ES (1) | ES2314248T3 (de) |
| GB (1) | GB0216648D0 (de) |
| PT (1) | PT1525320E (de) |
| SI (1) | SI1525320T1 (de) |
| WO (1) | WO2004009823A1 (de) |
Families Citing this family (86)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0216648D0 (en) * | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
| GB0319451D0 (en) * | 2003-08-19 | 2003-09-17 | Lonza Biologics Plc | Tropolone assay |
| SI1730191T1 (sl) | 2004-03-30 | 2011-11-30 | Glaxo Group Ltd | Imunoglobulin-vezavni hOSM |
| TWI307630B (en) | 2004-07-01 | 2009-03-21 | Glaxo Group Ltd | Immunoglobulins |
| US20090181424A1 (en) * | 2005-04-22 | 2009-07-16 | Fernando Albericio | Mammalian Expression Vector Comprising the MCMV Promoter and First Intron of HCMV Major Immediate Early Gene |
| EP1915394B1 (de) * | 2005-05-20 | 2021-01-27 | Lonza Biologics plc. | Expression auf hohem niveau von rekombinantem antikörper in einer säugetier-wirtszelle |
| WO2007014169A2 (en) | 2005-07-22 | 2007-02-01 | Y's Therapeutics Co, Ltd. | Anti-cd26 antibodies and methods of use thereof |
| US9884899B2 (en) | 2007-07-06 | 2018-02-06 | Promedior, Inc. | Methods for treating fibrosis using CRP antagonists |
| HUE037162T2 (hu) | 2007-07-09 | 2018-08-28 | Genentech Inc | Diszulfidkötés redukciójának megelõzése polipeptidek rekombináns elõállítása során |
| AR074777A1 (es) | 2008-12-19 | 2011-02-09 | Glaxo Group Ltd | Proteinas de union a antigeno |
| US10702583B2 (en) | 2009-03-11 | 2020-07-07 | Promedior, Inc. | Treatment methods for autoimmune disorders |
| JP5797565B2 (ja) | 2009-03-11 | 2015-10-21 | プロメディオール， インコーポレイテッド | 過敏性障害（ｈｙｐｅｒｓｅｎｓｉｔｉｖｅ ｄｉｓｏｒｄｅｒ）に対する処置および診断方法 |
| UA110323C2 (en) | 2009-06-04 | 2015-12-25 | Promedior Inc | Derivative of serum amyloid p and their receipt and application |
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| AR080301A1 (es) | 2010-02-24 | 2012-03-28 | Immunogen Inc | Anticuerpos e inmunoconjugados del receptor 1 de folato y sus usos |
| UA108227C2 (xx) | 2010-03-03 | 2015-04-10 | Антигензв'язуючий білок | |
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| AR081556A1 (es) | 2010-06-03 | 2012-10-03 | Glaxo Group Ltd | Proteinas de union al antigeno humanizadas |
| JP5883443B2 (ja) | 2010-07-22 | 2016-03-15 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | 新規の抗原結合タンパク質 |
| WO2012019024A2 (en) | 2010-08-04 | 2012-02-09 | Immunogen, Inc. | Her3-binding molecules and immunoconjugates thereof |
| PT2726101T (pt) | 2011-06-30 | 2018-11-21 | Genzyme Corp | Inibidores da ativação de células t |
| JP6185463B2 (ja) | 2011-07-15 | 2017-08-23 | オンコメッド ファーマシューティカルズ インコーポレイテッド | Ｒｓｐｏ結合剤およびその使用法 |
| US9549981B2 (en) | 2011-07-19 | 2017-01-24 | Philogen S.P.A. | Sequential antibody therapy |
| UY34317A (es) | 2011-09-12 | 2013-02-28 | Genzyme Corp | Anticuerpo antireceptor de célula T (alfa)/ß |
| SI3485903T1 (sl) | 2011-09-23 | 2023-02-28 | Mereo Biopharma 5, Inc. | Sredstva, ki vežejo VEGF/DLL4, in njihove uporabe |
| MX350957B (es) | 2011-11-23 | 2017-09-27 | Medimmune Llc | Moleculas de union especificas para her3 y usos de las mismas. |
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| EP2674495A1 (de) * | 2012-06-14 | 2013-12-18 | Sanofi | CHO-Expressionssystem |
| EA032038B1 (ru) | 2012-07-13 | 2019-03-29 | Онкомед Фармасьютикалс, Инк. | Rspo3-связывающие агенты и их применение |
| KR20150046316A (ko) | 2012-08-31 | 2015-04-29 | 이뮤노젠 아이엔씨 | 엽산 수용체 1의 검출을 위한 진단성 검정 및 키트 |
| EP2711426B1 (de) | 2012-09-24 | 2015-04-08 | Lonza Biologics plc | Expressionsvektoren mit chimärem Cytomegalieviruspromotor und Verstärkersequenzen |
| AU2013355379B2 (en) | 2012-12-04 | 2018-08-09 | Oncomed Pharmaceuticals, Inc. | Immunotherapy with binding agents |
| US20160194660A1 (en) * | 2012-12-21 | 2016-07-07 | Merck Sharp & Dohme Corp. | Expression vectors for recombinant protein production in mammalian cells |
| PT3038650T (pt) | 2013-08-30 | 2021-08-25 | Immunogen Inc | Anticorpos e ensaios para a deteção do recetor de folato 1 |
| EP3069137A1 (de) | 2013-11-05 | 2016-09-21 | Novartis Ag | Organische verbindungen |
| WO2015084808A1 (en) | 2013-12-02 | 2015-06-11 | Oncomed Pharmaceuticals, Inc. | Identification of predictive biomarkers associated with wnt pathway inhibitors |
| WO2015114150A1 (en) | 2014-02-02 | 2015-08-06 | Medimmune Limited | Chimeric protein composed of ngf antagonist domain and a tnfa antagonist domain |
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| SI3128997T1 (sl) | 2014-04-08 | 2020-11-30 | Boston Pharmaceuticals Inc. | Vezavne molekule specifične za IL-21 in njihove uporabe |
| SG11201608192SA (en) | 2014-04-11 | 2016-10-28 | Medimmune Llc | Bispecific her2 antibodies |
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| EP3148573A4 (de) | 2014-05-29 | 2017-11-15 | Medimmune, LLC | Ox40l-fusionsproteine und verwendungen davon |
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| CN104195173B (zh) * | 2014-09-02 | 2017-03-08 | 北京比洋生物技术有限公司 | 具有双表达盒的谷氨酰胺合成酶高效表达载体 |
| TW201619200A (zh) | 2014-10-10 | 2016-06-01 | 麥迪紐有限責任公司 | 人類化抗－ｏｘ４０抗體及其用途 |
| WO2016059602A2 (en) | 2014-10-16 | 2016-04-21 | Glaxo Group Limited | Methods of treating cancer and related compositions |
| KR102781223B1 (ko) | 2014-11-10 | 2025-03-12 | 메디뮨 리미티드 | Cd73에 특이적인 결합 분자 및 이의 용도 |
| US20160129108A1 (en) | 2014-11-11 | 2016-05-12 | Medimmune Limited | Therapeutic combinations comprising anti-cd73 antibodies and uses thereof |
| CN104561091A (zh) * | 2014-12-17 | 2015-04-29 | 华南理工大学 | 一种光学分析检测cho细胞增殖的方法及应用 |
| CN105779501A (zh) * | 2014-12-26 | 2016-07-20 | 上海药明康德新药开发有限公司 | 运用cho宿主细胞进行重组蛋白的瞬时表达生产的方法 |
| CN105802995A (zh) * | 2014-12-31 | 2016-07-27 | 上海复宏汉霖生物技术有限公司 | 一种利用cho细胞生产重组蛋白的基因表达系统及真核表达载体 |
| SG11201705598VA (en) | 2015-01-09 | 2017-08-30 | Medimmune Llc | Assay to detect human dpp-4 |
| CN107849111B (zh) | 2015-05-04 | 2022-02-22 | Vcn生物科学有限公司 | 在免疫显性腺病毒抗原表位中具有突变的溶瘤腺病毒及其在癌症治疗中的用途 |
| CN108026172B (zh) | 2015-06-26 | 2022-04-29 | 赛诺菲生物技术公司 | 单克隆抗il-1racp抗体 |
| SMT202100092T1 (it) | 2015-06-29 | 2021-03-15 | Immunogen Inc | Anticorpi anti-cd123 e loro coniugati e derivati |
| WO2017025610A1 (en) | 2015-08-12 | 2017-02-16 | Medimmune Limited | Gitrl fusion proteins and uses thereof |
| ES2968074T3 (es) | 2015-09-23 | 2024-05-07 | Mereo Biopharma 5 Inc | Anticuerpo bi-específico anti-VEGF/DLL4 para su uso en el tratamiento del cáncer de ovario resistente al platino |
| IL258537B2 (en) | 2015-10-09 | 2024-05-01 | Genzyme Corp | Improved flare (flow cytometry attenuated reporter expression) technology for rapid bulk sorting |
| LT3374398T (lt) | 2015-11-10 | 2020-09-10 | Medimmune, Llc | Asct2 atžvilgiu specifinės surišančios molekulės ir jų panaudojimai |
| CA3015277A1 (en) | 2016-03-10 | 2017-09-14 | Acceleron Pharma Inc. | Activin type 2 receptor binding proteins and uses thereof |
| CA3020839A1 (en) | 2016-04-15 | 2017-10-19 | Alder Biopharmaceuticals, Inc. | Anti-pacap antibodies and uses thereof |
| CN116217731B (zh) | 2016-04-22 | 2025-12-30 | 艾科赛扬制药股份有限公司 | Alk7结合蛋白及其用途 |
| PE20190622A1 (es) | 2016-06-02 | 2019-04-26 | Abbvie Inc | Agonista del receptor de glucocorticoides e inmunoconjugados del mismo |
| CN118497271A (zh) | 2016-10-07 | 2024-08-16 | 建新公司 | 用于生物制品生产的细胞的早期转染后分离(epic) |
| EP3525813B1 (de) | 2016-10-11 | 2024-04-17 | Razi Holdings, Inc. | Breit neutralisierende antikörper gegen die interne glycoproteinfusionsschleife des ebolavirus |
| CN110662770A (zh) | 2016-11-23 | 2020-01-07 | 比奥维拉迪维治疗股份有限公司 | 结合凝血因子ix和凝血因子x的双特异性抗体 |
| CN107973841B (zh) * | 2016-12-23 | 2022-03-25 | 浙江海隆生物科技有限公司 | Cho细胞表达的重组牛病毒性腹泻病毒e2蛋白及亚单位疫苗的制备方法和应用 |
| WO2018150269A1 (en) | 2017-02-17 | 2018-08-23 | Lonza Ltd. | Multi-site specific integration cells for difficult to express proteins |
| GB201703418D0 (en) | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for cell line development |
| US12054733B2 (en) | 2017-06-16 | 2024-08-06 | Lonza Ltd | Universal self-regulating mammalian cell line platform for the production of biologics |
| MA49796A (fr) | 2017-12-01 | 2020-06-03 | Abbvie Inc | Agoniste du récepteur des glucocorticoïdes et immunoconjugués de celui-ci |
| CN113227135B (zh) | 2018-12-28 | 2025-03-21 | 斯帕克斯治疗公司 | 用于治疗癌症和其他疾病的对紧密连接蛋白18.2具有特异性的结合分子、其组合物和方法 |
| MA55529A (fr) | 2019-04-03 | 2022-02-09 | Genzyme Corp | Polypeptides de liaison anti-alpha bêta tcr à fragmentation réduite |
| CN114269154A (zh) | 2019-06-18 | 2022-04-01 | 联合治疗公司 | 移植器官的线粒体处理 |
| CN113005140B (zh) * | 2019-12-19 | 2024-03-15 | 鲁南制药集团股份有限公司 | 一种具有双表达盒的gs表达载体及其应用 |
| BR112022024644A2 (pt) * | 2020-06-02 | 2023-02-23 | Catalent Pharma Solutions Llc | Linhagens celulares com múltiplas ancoragens para inserção de gene |
| JPWO2021251340A1 (de) | 2020-06-08 | 2021-12-16 | ||
| CN117279942A (zh) | 2020-09-28 | 2023-12-22 | 免疫医疗有限公司 | 用于治疗疼痛的化合物和方法 |
| JP7855319B2 (ja) | 2021-05-31 | 2026-05-08 | ワイズ・エー・シー株式会社 | 抗ｃｄ２６抗体と免疫チェックポイント阻害剤との併用療法 |
| US11807685B2 (en) | 2021-08-05 | 2023-11-07 | The Uab Research Foundation | Anti-CD47 antibody and uses thereof |
| WO2023064947A1 (en) | 2021-10-15 | 2023-04-20 | Regenxbio Inc. | Antibodies and methods of using thereof |
| EP4590704A1 (de) | 2022-09-21 | 2025-07-30 | Sanofi Biotechnology | Humanisierter anti-il-1r3-antikörper und verfahren zur verwendung |
| EP4667497A1 (de) | 2023-02-13 | 2025-12-24 | Shaoxing Institute, Zhejiang University | Bispezifischer antikörper und verwendung davon |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2944850A1 (de) * | 1979-11-07 | 1981-05-27 | Bayer Ag, 5090 Leverkusen | Mittel zur regulierung des pflanzenwachstums, deren herstellung und deren verwendung |
| US4968615A (en) | 1985-12-18 | 1990-11-06 | Ciba-Geigy Corporation | Deoxyribonucleic acid segment from a virus |
| US4963481A (en) * | 1985-12-18 | 1990-10-16 | Genetics Institute Inc | Promoter system |
| GB8601597D0 (en) | 1986-01-23 | 1986-02-26 | Wilson R H | Nucleotide sequences |
| US5891693A (en) * | 1990-01-25 | 1999-04-06 | Alusuisse Holdings A.G. | Recombinant DNA methods vectors and host cells |
| IT1262545B (it) | 1993-10-25 | 1996-07-02 | Menarini Ricerche Sud S P A A | Sistema di espressione per linee cellulari eucariotiche |
| DK0731845T3 (da) * | 1993-12-23 | 2003-10-20 | Merck & Co Inc | Homologt rekombinant antistofekspressionssystem til murine celler |
| CA2182398C (en) | 1994-02-07 | 2003-01-07 | Metin Colpan | Nucleic acid transfection efficiency increase by use of isopropanol in aqueous solutions |
| GB0216648D0 (en) * | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
| ATE399872T1 (de) | 2003-03-11 | 2008-07-15 | Serono Lab | Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten |
-
2002
- 2002-07-18 GB GBGB0216648.6A patent/GB0216648D0/en not_active Ceased
-
2003
- 2003-07-21 SI SI200331477T patent/SI1525320T1/sl unknown
- 2003-07-21 CA CA2489016A patent/CA2489016C/en not_active Expired - Lifetime
- 2003-07-21 ES ES03765075T patent/ES2314248T3/es not_active Expired - Lifetime
- 2003-07-21 CN CNB038169142A patent/CN1296486C/zh not_active Expired - Lifetime
- 2003-07-21 PT PT03765075T patent/PT1525320E/pt unknown
- 2003-07-21 DK DK03765075T patent/DK1525320T3/da active
- 2003-07-21 CA CA2672809A patent/CA2672809C/en not_active Expired - Lifetime
- 2003-07-21 WO PCT/EP2003/007946 patent/WO2004009823A1/en not_active Ceased
- 2003-07-21 DE DE60324430T patent/DE60324430D1/de not_active Expired - Lifetime
- 2003-07-21 EP EP03765075A patent/EP1525320B1/de not_active Expired - Lifetime
- 2003-07-21 US US10/521,768 patent/US20060003405A1/en not_active Abandoned
- 2003-07-21 AU AU2003251434A patent/AU2003251434B2/en not_active Expired
- 2003-07-21 AT AT03765075T patent/ATE412761T1/de active
-
2007
- 2007-12-21 US US11/962,261 patent/US7932087B2/en not_active Expired - Lifetime
-
2009
- 2009-02-19 AU AU2009200665A patent/AU2009200665B2/en not_active Expired
-
2010
- 2010-12-10 US US12/964,918 patent/US20110104745A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ES2314248T3 (es) | 2009-03-16 |
| EP1525320B1 (de) | 2008-10-29 |
| US20110104745A1 (en) | 2011-05-05 |
| WO2004009823A1 (en) | 2004-01-29 |
| EP1525320A1 (de) | 2005-04-27 |
| AU2009200665A1 (en) | 2009-03-12 |
| US20080138902A1 (en) | 2008-06-12 |
| US20060003405A1 (en) | 2006-01-05 |
| CA2672809A1 (en) | 2004-01-29 |
| US7932087B2 (en) | 2011-04-26 |
| AU2003251434B2 (en) | 2009-03-05 |
| DK1525320T3 (da) | 2009-02-23 |
| CA2672809C (en) | 2013-01-08 |
| PT1525320E (pt) | 2009-01-23 |
| AU2003251434A1 (en) | 2004-02-09 |
| CN1668749A (zh) | 2005-09-14 |
| SI1525320T1 (sl) | 2009-02-28 |
| HK1081595A1 (en) | 2006-05-19 |
| AU2009200665B2 (en) | 2012-08-16 |
| CA2489016C (en) | 2012-01-17 |
| GB0216648D0 (en) | 2002-08-28 |
| CN1296486C (zh) | 2007-01-24 |
| DE60324430D1 (de) | 2008-12-11 |
| CA2489016A1 (en) | 2004-01-29 |
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