DK1525320T3 - Fremgangsmåde til ekspression af rekombinant protein i CHO-celler - Google Patents
Fremgangsmåde til ekspression af rekombinant protein i CHO-cellerInfo
- Publication number
- DK1525320T3 DK1525320T3 DK03765075T DK03765075T DK1525320T3 DK 1525320 T3 DK1525320 T3 DK 1525320T3 DK 03765075 T DK03765075 T DK 03765075T DK 03765075 T DK03765075 T DK 03765075T DK 1525320 T3 DK1525320 T3 DK 1525320T3
- Authority
- DK
- Denmark
- Prior art keywords
- cho cells
- expression
- recombinant protein
- enhancing
- expression vector
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0216648.6A GB0216648D0 (en) | 2002-07-18 | 2002-07-18 | Method of expressing recombinant protein in CHO cells |
| PCT/EP2003/007946 WO2004009823A1 (en) | 2002-07-18 | 2003-07-21 | Method of expressing recombinant protein in cho cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1525320T3 true DK1525320T3 (da) | 2009-02-23 |
Family
ID=9940670
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK03765075T DK1525320T3 (da) | 2002-07-18 | 2003-07-21 | Fremgangsmåde til ekspression af rekombinant protein i CHO-celler |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US20060003405A1 (da) |
| EP (1) | EP1525320B1 (da) |
| CN (1) | CN1296486C (da) |
| AT (1) | ATE412761T1 (da) |
| AU (2) | AU2003251434B2 (da) |
| CA (2) | CA2489016C (da) |
| DE (1) | DE60324430D1 (da) |
| DK (1) | DK1525320T3 (da) |
| ES (1) | ES2314248T3 (da) |
| GB (1) | GB0216648D0 (da) |
| PT (1) | PT1525320E (da) |
| SI (1) | SI1525320T1 (da) |
| WO (1) | WO2004009823A1 (da) |
Families Citing this family (88)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0216648D0 (en) * | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
| GB0319451D0 (en) * | 2003-08-19 | 2003-09-17 | Lonza Biologics Plc | Tropolone assay |
| BRPI0509367A (pt) | 2004-03-30 | 2007-09-11 | Glaxo Group Ltd | anticorpo, fragmento de ligação de antìgeno, composição farmacêutica, métodos de tratar um paciente humano afligido com uma doença ou distúrbio, de triar um anticorpo, e de detectar hosm em uma amostra biológica, uso de um anticorpo terapêutico ou fragmento de ligação de antìgeno, medicamento, vetor, polunucleotìdeo, célula hospedeira recombinante estavelmente transformada ou transfectada, e, processo para a fabricação de um anticorpo terapêutico |
| TWI307630B (en) | 2004-07-01 | 2009-03-21 | Glaxo Group Ltd | Immunoglobulins |
| WO2006111387A2 (en) * | 2005-04-22 | 2006-10-26 | Lonza Biologics Plc. | Mammalian expression vector comprising the mcmv promoter and first intron of hcmv major immediate early gene |
| US20090215165A1 (en) * | 2005-05-20 | 2009-08-27 | James Rance | High-level expression of recombinant antibody in a mammalian host cell |
| RU2486204C2 (ru) | 2005-07-22 | 2013-06-27 | Уай'З Терапьютикс Ко., Лтд. | Антитела против сd26 и способы их применения |
| US9884899B2 (en) | 2007-07-06 | 2018-02-06 | Promedior, Inc. | Methods for treating fibrosis using CRP antagonists |
| EP2586788B1 (en) | 2007-07-09 | 2017-11-29 | Genentech, Inc. | Prevention of disulfide bond reduction during recombinant production of polypeptides |
| TW201029662A (en) | 2008-12-19 | 2010-08-16 | Glaxo Group Ltd | Novel antigen binding proteins |
| PT2405928T (pt) | 2009-03-11 | 2017-02-07 | Promedior Inc | Métodos de tratamento e de diagnóstico para patologias de hipersensibilidade |
| PT2405929T (pt) | 2009-03-11 | 2018-07-23 | Promedior Inc | Métodos de tratamento para distúrbios autoimunes |
| UA110323C2 (en) | 2009-06-04 | 2015-12-25 | Promedior Inc | Derivative of serum amyloid p and their receipt and application |
| HUE041034T2 (hu) | 2009-06-17 | 2019-05-28 | Promedior Inc | SAP-variánsok és alkalmazásuk |
| MX355650B (es) * | 2009-08-11 | 2018-04-26 | Genentech Inc | Produccion de proteinas en medios de cultivo de celulas libres de glutamina. |
| WO2011080050A2 (en) | 2009-12-11 | 2011-07-07 | Novartis Ag | Binding molecules |
| CA2787070A1 (en) | 2010-01-28 | 2011-08-04 | Ian Kirby | Cd127 binding proteins |
| CN105777907B (zh) | 2010-02-24 | 2020-03-17 | 伊缪诺金公司 | 叶酸受体1抗体与免疫缀合物以及其用途 |
| UA108227C2 (xx) | 2010-03-03 | 2015-04-10 | Антигензв'язуючий білок | |
| LT2544719T (lt) | 2010-03-12 | 2019-10-25 | Debiopharm Int Sa | Cd37 surišančios molekulės, ir jų imunokonjugatai |
| AR081556A1 (es) | 2010-06-03 | 2012-10-03 | Glaxo Group Ltd | Proteinas de union al antigeno humanizadas |
| US8987423B2 (en) | 2010-07-22 | 2015-03-24 | Glaxosmithkline Biologicals, S.A. | MAGE antigen binding proteins |
| WO2012019024A2 (en) | 2010-08-04 | 2012-02-09 | Immunogen, Inc. | Her3-binding molecules and immunoconjugates thereof |
| RU2657440C2 (ru) | 2011-06-30 | 2018-06-13 | Джензим Корпорейшн | Ингибиторы т-клеточной активации |
| WO2013012747A1 (en) | 2011-07-15 | 2013-01-24 | Oncomed Pharmaceuticals, Inc. | Rspo binding agents and uses thereof |
| US9549981B2 (en) | 2011-07-19 | 2017-01-24 | Philogen S.P.A. | Sequential antibody therapy |
| UY34317A (es) | 2011-09-12 | 2013-02-28 | Genzyme Corp | Anticuerpo antireceptor de célula T (alfa)/ß |
| TWI583699B (zh) | 2011-09-23 | 2017-05-21 | 安可美德藥物股份有限公司 | Ｖｅｇｆ／ｄｌｌ４結合劑類及彼等之用途 |
| CN108424456B (zh) | 2011-11-23 | 2022-04-26 | 医学免疫有限责任公司 | 特异于her3的结合分子及其用途 |
| MX2014014951A (es) | 2012-06-06 | 2015-03-13 | Oncomed Pharm Inc | Agentes aglutinantes que modulan la trayectoria hippo y sus usos. |
| EP2674495A1 (en) * | 2012-06-14 | 2013-12-18 | Sanofi | CHO expression system |
| CN104854132A (zh) | 2012-07-13 | 2015-08-19 | 昂考梅德药品有限公司 | Rspo3结合剂及其用途 |
| SG11201500938XA (en) | 2012-08-31 | 2015-04-29 | Immunogen Inc | Diagnostic assays and kits for detection of folate receptor 1 |
| HUE026516T2 (en) | 2012-09-24 | 2016-06-28 | Lonza Biologics Plc | Expression vectors containing chimeric cytomegalovirus promoter and enhancer sequences |
| WO2014089169A2 (en) | 2012-12-04 | 2014-06-12 | Oncomed Pharmaceuticals, Inc. | Immunotherapy with binding agents |
| US20160194660A1 (en) * | 2012-12-21 | 2016-07-07 | Merck Sharp & Dohme Corp. | Expression vectors for recombinant protein production in mammalian cells |
| NZ717140A (en) | 2013-08-30 | 2022-05-27 | Immunogen Inc | Antibodies and assays for detection of folate receptor 1 |
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| CA2938066C (en) | 2014-02-02 | 2025-05-06 | Medimmune Limited | CHIMERIC PROTEIN COMPOSED OF NERVE GROWTH FACTOR ANTAGONIST DOMAIN AND TUMOR NECROSIS FACTOR-ANTAGONIST DOMAIN-A |
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| WO2016059602A2 (en) | 2014-10-16 | 2016-04-21 | Glaxo Group Limited | Methods of treating cancer and related compositions |
| RS62003B2 (sr) | 2014-11-10 | 2025-01-31 | Medimmune Ltd | Vezujući molekuli specifični za cd73 i njihove upotrebe |
| EP3789403A1 (en) | 2014-11-11 | 2021-03-10 | MedImmune Limited | Therapeutic combinations comprising anti-cd73 antibodies and a2a receptor inhibitor and uses thereof |
| CN104561091A (zh) * | 2014-12-17 | 2015-04-29 | 华南理工大学 | 一种光学分析检测cho细胞增殖的方法及应用 |
| CN105779501A (zh) * | 2014-12-26 | 2016-07-20 | 上海药明康德新药开发有限公司 | 运用cho宿主细胞进行重组蛋白的瞬时表达生产的方法 |
| CN105802995A (zh) * | 2014-12-31 | 2016-07-27 | 上海复宏汉霖生物技术有限公司 | 一种利用cho细胞生产重组蛋白的基因表达系统及真核表达载体 |
| AU2016205188A1 (en) | 2015-01-09 | 2017-06-22 | Medimmune, Llc | Assay to detect human DPP-4 |
| CN107849111B (zh) | 2015-05-04 | 2022-02-22 | Vcn生物科学有限公司 | 在免疫显性腺病毒抗原表位中具有突变的溶瘤腺病毒及其在癌症治疗中的用途 |
| CN108026172B (zh) | 2015-06-26 | 2022-04-29 | 赛诺菲生物技术公司 | 单克隆抗il-1racp抗体 |
| SMT202100092T1 (it) | 2015-06-29 | 2021-03-15 | Immunogen Inc | Anticorpi anti-cd123 e loro coniugati e derivati |
| JP2018522571A (ja) | 2015-08-12 | 2018-08-16 | メディミューン リミテッド | Ｇｉｔｒｌ融合タンパク質およびその使用 |
| US11339213B2 (en) | 2015-09-23 | 2022-05-24 | Mereo Biopharma 5, Inc. | Methods and compositions for treatment of cancer |
| IL258538B2 (en) | 2015-10-09 | 2023-04-01 | Genzyme Corp | Early isolation after the infection of cells (epic) for the production of biological material |
| PL3374398T3 (pl) | 2015-11-10 | 2020-08-24 | Medimmune, Llc | Cząsteczki wiążące swoiste dla ASCT2 i ich zastosowanie |
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| SG10202109691UA (en) | 2016-04-15 | 2021-10-28 | Alder Biopharmaceuticals Inc | Anti-pacap antibodies and uses thereof |
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| RS62040B1 (sr) | 2016-06-02 | 2021-07-30 | Abbvie Inc | Agonist receptora glukokortikoida i njegovi imunokonjugati |
| ES3021241T3 (en) | 2016-10-07 | 2025-05-26 | Genzyme Corp | Early post-transfection isolation of cells (epic) for biologics production |
| EP3525813B1 (en) | 2016-10-11 | 2024-04-17 | Razi Holdings, Inc. | Broadly neutralizing antibody targeting the ebolavirus glycoprotein internal fusion loop |
| AU2017363309A1 (en) | 2016-11-23 | 2019-07-11 | Bioverativ Therapeutics Inc. | Mono- and bispecific antibodies binding to coagulation factor IX and coagulation factor X |
| CN107973841B (zh) * | 2016-12-23 | 2022-03-25 | 浙江海隆生物科技有限公司 | Cho细胞表达的重组牛病毒性腹泻病毒e2蛋白及亚单位疫苗的制备方法和应用 |
| CN119242587A (zh) | 2017-02-17 | 2025-01-03 | 隆萨有限公司 | 难以表达的蛋白质的多位点特异性整合细胞 |
| GB201703418D0 (en) | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for cell line development |
| EP3401332A1 (en) | 2017-05-08 | 2018-11-14 | MAB Discovery GmbH | Anti-il-1r3 antibodies for use in inflammatory conditions |
| US12054733B2 (en) | 2017-06-16 | 2024-08-06 | Lonza Ltd | Universal self-regulating mammalian cell line platform for the production of biologics |
| EP3658192B1 (en) | 2017-12-01 | 2021-04-28 | AbbVie Inc. | Glucocorticoid receptor agonist and immunoconjugates thereof |
| US11447551B2 (en) | 2018-12-28 | 2022-09-20 | Sparx Bioscience Limited | Binding molecules specific for claudin 18.2, compositions and methods thereof, for the treatment of cancer and other diseases |
| JP7575401B2 (ja) | 2019-04-03 | 2024-10-29 | ジェンザイム・コーポレーション | 断片化が低減した抗アルファベータｔｃｒ結合ポリペプチド |
| WO2020257281A1 (en) | 2019-06-18 | 2020-12-24 | United Therapeutics Corporation | Mitochondrial treatment of organs for transplantation |
| CN113005140B (zh) * | 2019-12-19 | 2024-03-15 | 鲁南制药集团股份有限公司 | 一种具有双表达盒的gs表达载体及其应用 |
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| US20230398116A1 (en) | 2020-06-08 | 2023-12-14 | Y's Ac Co., Ltd. | Agent for reversing resistance to anticancer drugs |
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| JP7855319B2 (ja) | 2021-05-31 | 2026-05-08 | ワイズ・エー・シー株式会社 | 抗ｃｄ２６抗体と免疫チェックポイント阻害剤との併用療法 |
| US11807685B2 (en) | 2021-08-05 | 2023-11-07 | The Uab Research Foundation | Anti-CD47 antibody and uses thereof |
| CA3234162A1 (en) | 2021-10-15 | 2023-04-20 | Michele Fiscella | Antibodies and methods of using thereof |
| US12054552B2 (en) | 2022-09-21 | 2024-08-06 | Sanofi Biotechnology | Humanized anti-IL-1R3 antibody and methods of use |
| WO2024169990A1 (zh) | 2023-02-13 | 2024-08-22 | 浙江大学绍兴研究院 | 双特异性抗体及其应用 |
| WO2026120069A1 (en) | 2024-12-05 | 2026-06-11 | H. Lundbeck A/S | Humanized anti-pacap antibody and uses thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2944850A1 (de) * | 1979-11-07 | 1981-05-27 | Bayer Ag, 5090 Leverkusen | Mittel zur regulierung des pflanzenwachstums, deren herstellung und deren verwendung |
| US4968615A (en) * | 1985-12-18 | 1990-11-06 | Ciba-Geigy Corporation | Deoxyribonucleic acid segment from a virus |
| US4963481A (en) * | 1985-12-18 | 1990-10-16 | Genetics Institute Inc | Promoter system |
| GB8601597D0 (en) * | 1986-01-23 | 1986-02-26 | Wilson R H | Nucleotide sequences |
| US5891693A (en) * | 1990-01-25 | 1999-04-06 | Alusuisse Holdings A.G. | Recombinant DNA methods vectors and host cells |
| IT1262545B (it) | 1993-10-25 | 1996-07-02 | Menarini Ricerche Sud S P A A | Sistema di espressione per linee cellulari eucariotiche |
| CZ289308B6 (cs) * | 1993-12-23 | 2001-12-12 | Merck & Co., Inc. | Homologní rekombinantní expresní vektor pro expresi rekombinantních genů a způsob exprese rekombinantních genů |
| ATE181921T1 (de) * | 1994-02-07 | 1999-07-15 | Qiagen Gmbh | Chromatographische isolierung von nucleinsäuren |
| GB0216648D0 (en) * | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
| ATE399872T1 (de) | 2003-03-11 | 2008-07-15 | Serono Lab | Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten |
-
2002
- 2002-07-18 GB GBGB0216648.6A patent/GB0216648D0/en not_active Ceased
-
2003
- 2003-07-21 ES ES03765075T patent/ES2314248T3/es not_active Expired - Lifetime
- 2003-07-21 CA CA2489016A patent/CA2489016C/en not_active Expired - Lifetime
- 2003-07-21 SI SI200331477T patent/SI1525320T1/sl unknown
- 2003-07-21 AT AT03765075T patent/ATE412761T1/de active
- 2003-07-21 CA CA2672809A patent/CA2672809C/en not_active Expired - Lifetime
- 2003-07-21 CN CNB038169142A patent/CN1296486C/zh not_active Expired - Lifetime
- 2003-07-21 PT PT03765075T patent/PT1525320E/pt unknown
- 2003-07-21 AU AU2003251434A patent/AU2003251434B2/en not_active Expired
- 2003-07-21 WO PCT/EP2003/007946 patent/WO2004009823A1/en not_active Ceased
- 2003-07-21 DK DK03765075T patent/DK1525320T3/da active
- 2003-07-21 US US10/521,768 patent/US20060003405A1/en not_active Abandoned
- 2003-07-21 DE DE60324430T patent/DE60324430D1/de not_active Expired - Lifetime
- 2003-07-21 EP EP03765075A patent/EP1525320B1/en not_active Expired - Lifetime
-
2007
- 2007-12-21 US US11/962,261 patent/US7932087B2/en not_active Expired - Lifetime
-
2009
- 2009-02-19 AU AU2009200665A patent/AU2009200665B2/en not_active Expired
-
2010
- 2010-12-10 US US12/964,918 patent/US20110104745A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE412761T1 (de) | 2008-11-15 |
| ES2314248T3 (es) | 2009-03-16 |
| WO2004009823A1 (en) | 2004-01-29 |
| US20080138902A1 (en) | 2008-06-12 |
| CN1296486C (zh) | 2007-01-24 |
| AU2009200665B2 (en) | 2012-08-16 |
| EP1525320B1 (en) | 2008-10-29 |
| PT1525320E (pt) | 2009-01-23 |
| CA2672809A1 (en) | 2004-01-29 |
| DE60324430D1 (de) | 2008-12-11 |
| EP1525320A1 (en) | 2005-04-27 |
| US20110104745A1 (en) | 2011-05-05 |
| US20060003405A1 (en) | 2006-01-05 |
| AU2009200665A1 (en) | 2009-03-12 |
| CA2672809C (en) | 2013-01-08 |
| US7932087B2 (en) | 2011-04-26 |
| HK1081595A1 (en) | 2006-05-19 |
| CA2489016A1 (en) | 2004-01-29 |
| CN1668749A (zh) | 2005-09-14 |
| AU2003251434B2 (en) | 2009-03-05 |
| CA2489016C (en) | 2012-01-17 |
| AU2003251434A1 (en) | 2004-02-09 |
| GB0216648D0 (en) | 2002-08-28 |
| SI1525320T1 (sl) | 2009-02-28 |
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