ATE423212T1 - Verfahren zur isolierung von polynukleotiden aus dem mycobakteriellen genom durch verwendung einer bac-basierenden dns-bibliothek: verwendung zur erkennung - Google Patents

Verfahren zur isolierung von polynukleotiden aus dem mycobakteriellen genom durch verwendung einer bac-basierenden dns-bibliothek: verwendung zur erkennung

Info

Publication number
ATE423212T1
ATE423212T1 AT99913534T AT99913534T ATE423212T1 AT E423212 T1 ATE423212 T1 AT E423212T1 AT 99913534 T AT99913534 T AT 99913534T AT 99913534 T AT99913534 T AT 99913534T AT E423212 T1 ATE423212 T1 AT E423212T1
Authority
AT
Austria
Prior art keywords
bac
polynucleotides
isolation
detection
dna library
Prior art date
Application number
AT99913534T
Other languages
English (en)
Inventor
Stewart Cole
Roland Buchrieser-Brosch
Stephen Gordon
Alain Billault
Original Assignee
Pasteur Institut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut filed Critical Pasteur Institut
Application granted granted Critical
Publication of ATE423212T1 publication Critical patent/ATE423212T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT99913534T 1998-04-16 1999-04-16 Verfahren zur isolierung von polynukleotiden aus dem mycobakteriellen genom durch verwendung einer bac-basierenden dns-bibliothek: verwendung zur erkennung ATE423212T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/060,756 US6183957B1 (en) 1998-04-16 1998-04-16 Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria

Publications (1)

Publication Number Publication Date
ATE423212T1 true ATE423212T1 (de) 2009-03-15

Family

ID=22031547

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99913534T ATE423212T1 (de) 1998-04-16 1999-04-16 Verfahren zur isolierung von polynukleotiden aus dem mycobakteriellen genom durch verwendung einer bac-basierenden dns-bibliothek: verwendung zur erkennung

Country Status (10)

Country Link
US (7) US6183957B1 (de)
EP (2) EP2058325A3 (de)
AT (1) ATE423212T1 (de)
AU (1) AU3164099A (de)
CY (1) CY1109058T1 (de)
DE (1) DE69940427D1 (de)
DK (1) DK1071797T3 (de)
ES (1) ES2322521T3 (de)
PT (1) PT1071797E (de)
WO (1) WO1999054487A2 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6183957B1 (en) 1998-04-16 2001-02-06 Institut Pasteur Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria
FR2791067B1 (fr) 1999-03-16 2003-01-31 Pasteur Institut Sequence deletees chez m.bovis bcg/m.ou m.tuberculosis, procede de detection des mycobacteries utilisant ces sequences et vaccins
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
WO2001077335A2 (fr) * 2000-04-11 2001-10-18 Institut Pasteur Genome de listeria monocytogenes, polypeptides et utilisations
US20020137043A1 (en) * 2000-08-26 2002-09-26 Nila Patil Method for reducing complexity of nucleic acid samples
US20020164634A1 (en) * 2000-08-26 2002-11-07 Perlegen Sciences, Inc. Methods for reducing complexity of nucleic acid samples
US20040121322A9 (en) * 2001-02-22 2004-06-24 Cole Stewart T. Comparative mycobacterial genomics as a tool for identifying targets for the diagnosis, prophylaxis or treatment of mycobacterioses
WO2003000721A2 (en) * 2001-06-22 2003-01-03 Health Protection Agency Mycobacterial antigens expressed under low oxygen tension
CA2453173C (en) * 2001-07-04 2013-12-10 Health Protection Agency Mycobacterial antigens expressed during latency
WO2003018053A1 (en) * 2001-08-22 2003-03-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vaccine against mycobacterial-induced diseases
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
EP1451318A4 (de) 2001-10-12 2007-06-27 Perkinelmer Las Inc Zusammenstellungen von nukleinsäuren und arrays und verfahren zu ihrer verwendung
GB0125535D0 (en) * 2001-10-24 2001-12-12 Microbiological Res Authority Mycobacterial genes down-regulated during latency
US20040023237A1 (en) * 2001-11-26 2004-02-05 Perelegen Sciences Inc. Methods for genomic analysis
US20090124514A1 (en) * 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US20060183132A1 (en) * 2005-02-14 2006-08-17 Perlegen Sciences, Inc. Selection probe amplification
US20050100911A1 (en) * 2003-08-06 2005-05-12 Perlegen Sciences, Inc. Methods for enriching populations of nucleic acid samples
ITRM20030411A1 (it) * 2003-08-29 2005-02-28 Consiglio Nazionale Ricerche Uso di sequenze geniche specifiche di mycobacterium tubercolosis ecorrispondenti proteine per la diagnosi e la prevenzione di infezione tubercolare.
WO2005044086A2 (en) * 2003-10-30 2005-05-19 Tufts-New England Medical Center Prenatal diagnosis using cell-free fetal dna in amniotic fluid
NZ554824A (en) 2004-10-22 2010-10-29 Revivicor Inc Porcines that lack endogenous antibody chains and express exogenous immunoglobulins
EP2305701A1 (de) * 2005-07-01 2011-04-06 Forsyth Dental Infirmary for Children Assays zur Detektion von Tuberkuloseantigenen und Impfstoffe
US20070172871A1 (en) * 2006-01-20 2007-07-26 Dishaw Larry J Methods for profiling transcriptosomes
RU2341288C1 (ru) * 2007-05-10 2008-12-20 Общество С Ограниченной Ответственностью "Биотэк" СРЕДСТВО, ОБЛАДАЮЩЕЕ СВОЙСТВОМ ФОРМИРОВАТЬ КЛЕТОЧНЫЙ ИММУНИТЕТ ПРОТИВ MYCOBACTERIUM TUBERCULOSIS H37 Rv, СПОСОБ ПОЛУЧЕНИЯ ЕГО (ВАРИАНТЫ), РЕКОМБИНАНТНЫЙ ШТАММ И СРЕДСТВО ДЛЯ ДИАГНОСТИКИ ТУБЕРКУЛЕЗА
RU2381274C2 (ru) * 2008-02-26 2010-02-10 Федеральное государственное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФГУН ГНЦ ВБ "Вектор" Роспотребнадзора) РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pТВ232, КОДИРУЮЩАЯ ГИБРИДНЫЙ ПОЛИПЕПТИД GST-CFP10 СО СВОЙСТВАМИ ВИДОСПЕЦИФИЧНОГО МИКОБАКТЕРИАЛЬНОГО АНТИГЕНА CFP10, РЕКОМБИНАНТНЫЙ ШТАММ БАКТЕРИЙ Escherichia coli - ПРОДУЦЕНТ ГИБРИДНОГО ПОЛИПЕПТИДА GST-CFP10 И РЕКОМБИНАНТНЫЙ ПОЛИПЕПТИД GST-CFP10
DK2348827T3 (en) 2008-10-27 2015-07-20 Revivicor Inc IMMUNICIPLY COMPROMATED PETS
RU2416428C2 (ru) * 2009-04-01 2011-04-20 Федеральное государственное образовательное учреждение высшего профессионального образования "Казанская государственная академия ветеринарной медицины им. Н.Э. Баумана" Способ получения антигена для диагностики туберкулеза крупного рогатого скота
US8932812B2 (en) * 2009-12-17 2015-01-13 Keygene N.V. Restriction enzyme based whole genome sequencing
US8865404B2 (en) * 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
EP3382040A3 (de) * 2013-12-03 2018-10-31 Kusuma School of Biological Sciences Genetische marker zur diagnose von durch mycobacterium tuberculosis herbeigeführter tuberkulose

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422956A1 (fr) 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
FR2518755B1 (fr) 1981-12-23 1986-04-11 Pasteur Institut Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5776693A (en) * 1990-06-08 1998-07-07 Institut Pasteur Specific detection of the mycobacterium tuberculosis
WO1993003187A2 (en) 1991-07-31 1993-02-18 Amoco Corporation Nucleic acid probes for the detection of shigella
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
DE69330750T2 (de) * 1992-03-04 2002-07-04 The Regents Of The University Of California, Oakland Vergleichende genomhybridisierung
EP0630408B1 (de) 1992-03-04 2003-01-02 Genencor International, Inc. Herstellung von -i(aspergillus niger katalase-r)
US6641814B1 (en) * 1997-04-02 2003-11-04 Statens Serum Institut Nucleic acids fragments and polypeptide fragments derived from M. tuberculosis
NL192999C (nl) 1994-11-17 1998-07-03 Lederverwerkende Ind De Buffel Koppeling.
CN1154730C (zh) * 1995-09-01 2004-06-23 科里克萨有限公司 用于结核病诊断的化合物和方法
GB9526178D0 (en) * 1995-12-21 1996-02-21 Taylor John Hermon Novel polynuceotides and polypeptides in pathogenic mycobacteria and their use as diagnostics,vaccines and targets for chemotherapy
JP2000509981A (ja) * 1996-04-29 2000-08-08 ゲゼルシャフト・フュア・ビオテクノロギッシェ・フォルシュンク・ミット・ベシュレンクテル・ハフツング（ゲー・ベー・エフ） マイコバクテリウム感染の検出のために有用なｄｎａ、ｒｎａ及びタンパク質
US6183957B1 (en) * 1998-04-16 2001-02-06 Institut Pasteur Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria

Also Published As

Publication number Publication date
US7112663B1 (en) 2006-09-26
DE69940427D1 (de) 2009-04-02
EP1071797A2 (de) 2001-01-31
DK1071797T3 (da) 2009-06-02
US6991904B2 (en) 2006-01-31
WO1999054487A3 (en) 1999-12-09
PT1071797E (pt) 2009-05-20
US6492506B1 (en) 2002-12-10
WO1999054487A2 (en) 1999-10-28
ES2322521T3 (es) 2009-06-22
EP2058325A3 (de) 2009-10-28
US20030198974A1 (en) 2003-10-23
AU3164099A (en) 1999-11-08
US20060063182A1 (en) 2006-03-23
EP1071797B1 (de) 2009-02-18
US20050250104A1 (en) 2005-11-10
CY1109058T1 (el) 2014-07-02
US6183957B1 (en) 2001-02-06
US20070092884A1 (en) 2007-04-26
EP2058325A2 (de) 2009-05-13
US7842781B2 (en) 2010-11-30
US8455187B2 (en) 2013-06-04

Similar Documents

Publication Publication Date Title
DE69940427D1 (de) Verfahren zur isolierung von polynukleotiden aus dem mycobakteriellen genom durch verwendung einer bac-basierenden dns-bibliothek: verwendung zur erkennung
Walsh et al. A rapid chemiluminescent method for quanititation of human DNA
ATE147794T1 (de) Chemisches verfahren zur analyse von dns- sequenzen
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
DE602005020943D1 (de) Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe
CA2172248A1 (en) Self-sustained sequence replication electrochemiluminescent nucleic acid assay
WO2000077260A8 (en) Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
DE60128908D1 (de) Verfahren zum Nachweis und zur Lokalisierung von Genen in situ durch Hybridisierung einer verzweigten DNA
DE69931608D1 (de) Verfahren zur bestimmung von dna-längenpolymorphismen
DE69619247D1 (de) Verfahren zur sequenzierung von nukleinsäuren
WO2001071042A8 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
ATE110418T1 (de) Nachweis von mykoplasma durch hybridisierung von dns.
ATE222606T1 (de) Multiplex-pcr zum nachweis von infektionen verursacht durch mycobacterium avium komplex
WO2003057913A3 (fr) Procede de detection et/ou d'identification de l'espece animale d'origine de la matiere animale contenue dans un echantillon
ATE474933T1 (de) Verfahren zum nachweis von pathogenen organismen
EP0717780B1 (de) Detektierung und artbestimmung von campylobacter
WO2001014592A3 (en) Identifying organisms by detecting intronic nucleic acid or encoded proteins
EP0739987A3 (de) Oligonukleotide und Verfahren für den Nachweis von Salmonella
ATE90971T1 (de) Methode zur analyse einer spezifischen sequenz von dns oder rns, reagenzien und kits dafuer.
ATE431432T1 (de) Verfahren zum nachweis von mycobacterium tuberculosis
WO1999000517A3 (en) Biosensor materials and methods
WO2005059505A3 (es) Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real
DE50204689D1 (de) Verfahren zum nachweis von gram-positiven bakterien
GR3036316T3 (en) Method, test element and test kit for semi-quantitative detection of target nucleic acid.
AU3081500A (en) Dna oligonucleotide which is specific for meloidogyne species, dna vector and host cell containing the oligonucleotide, use and kit

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1071797

Country of ref document: EP