ATE431433T1 - Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen - Google Patents

Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen

Info

Publication number
ATE431433T1
ATE431433T1 AT05760637T AT05760637T ATE431433T1 AT E431433 T1 ATE431433 T1 AT E431433T1 AT 05760637 T AT05760637 T AT 05760637T AT 05760637 T AT05760637 T AT 05760637T AT E431433 T1 ATE431433 T1 AT E431433T1
Authority
AT
Austria
Prior art keywords
present
encoding
reaction
ligation
sample
Prior art date
Application number
AT05760637T
Other languages
English (en)
Inventor
Mark R Andersen
Kenneth J Livak
Adam Broomer
Caifu Chen
Original Assignee
Applied Biosystems Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Biosystems Llc filed Critical Applied Biosystems Llc
Application granted granted Critical
Publication of ATE431433T1 publication Critical patent/ATE431433T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT05760637T 2004-03-24 2005-03-24 Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen ATE431433T1 (de)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US55622404P 2004-03-24 2004-03-24
US55616204P 2004-03-24 2004-03-24
US55615704P 2004-03-24 2004-03-24
US55616304P 2004-03-24 2004-03-24
US63068104P 2004-11-24 2004-11-24
PCT/US2005/010184 WO2005098040A2 (en) 2004-03-24 2005-03-24 Ligation and amplification reactions for determining target molecules

Publications (1)

Publication Number Publication Date
ATE431433T1 true ATE431433T1 (de) 2009-05-15

Family

ID=35064420

Family Applications (2)

Application Number Title Priority Date Filing Date
AT05732398T ATE399884T1 (de) 2004-03-24 2005-03-24 Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden
AT05760637T ATE431433T1 (de) 2004-03-24 2005-03-24 Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT05732398T ATE399884T1 (de) 2004-03-24 2005-03-24 Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden

Country Status (6)

Country Link
US (2) US7604937B2 (de)
EP (3) EP1730312B1 (de)
JP (2) JP2007530051A (de)
AT (2) ATE399884T1 (de)
DE (2) DE602005007874D1 (de)
WO (2) WO2005094532A2 (de)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153658B2 (en) * 2002-09-19 2006-12-26 Applera Corporation Methods and compositions for detecting targets
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
EP1730312B1 (de) 2004-03-24 2008-07-02 Applera Corporation Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden
US7364855B2 (en) * 2004-04-30 2008-04-29 Applera Corporation Methods and kits for methylation detection
US7427479B2 (en) * 2004-04-30 2008-09-23 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
WO2006005055A2 (en) * 2004-06-30 2006-01-12 Applera Corporation Methods reaction mixtures and kits for ligating polynucleotides
WO2006034387A1 (en) * 2004-09-21 2006-03-30 Applera Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs)
DE602006018472D1 (de) 2005-07-15 2011-01-05 Life Technologies Corp Analyse von boten-rna und mikro-rna im gleichen reaktionsansatz
US20070026439A1 (en) * 2005-07-15 2007-02-01 Applera Corporation Fluid processing device and method
US20070068573A1 (en) * 2005-08-22 2007-03-29 Applera Corporation Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible
WO2007025281A2 (en) 2005-08-24 2007-03-01 Applera Corporation A method to quantify sirnas, mirnas and polymorphic mirnas
WO2007133703A2 (en) * 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US8597938B2 (en) * 2007-10-12 2013-12-03 Qiagen Sciences Llc System for providing control reactions for real time RT-PCR
WO2009100449A1 (en) * 2008-02-08 2009-08-13 Fluidigm Corporation Dynamic array assay methods
WO2010027870A2 (en) * 2008-08-26 2010-03-11 Fluidigm Corporation Assay methods for increased throughput of samples and/or targets
EP3216874A1 (de) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Verfahren zum stratifizieren und annotieren der behandlungsoptionen eines krebsmedikaments
JP5608998B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
WO2010114599A1 (en) * 2009-04-01 2010-10-07 Dx Terity Diagnostics Inc. Chemical ligation dependent probe amplification (clpa)
CN103952482A (zh) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 用于对目标核酸进行条形码化的多引物扩增方法
WO2011056866A2 (en) 2009-11-05 2011-05-12 Epicentre Technologies Corporation Methods and kits for 3'-end-tagging of rna
WO2011071923A2 (en) * 2009-12-07 2011-06-16 Illumina, Inc. Multi-sample indexing for multiplex genotyping
EP2534263B1 (de) * 2010-02-09 2020-08-05 Unitaq Bio Verfahren und zusammensetzungen zur universellen erkennung von nukleinsäuren
US10066257B2 (en) 2010-03-29 2018-09-04 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
AU2011305445B2 (en) 2010-09-24 2017-03-16 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
US9353406B2 (en) * 2010-10-22 2016-05-31 Fluidigm Corporation Universal probe assay methods
US20120288857A1 (en) * 2011-02-03 2012-11-15 Fluidigm Corporation Multifunctional probe-primers
US20140170653A1 (en) 2011-04-15 2014-06-19 Life Technologies Corporation Chemical ligation
US8809238B2 (en) 2011-05-09 2014-08-19 Fluidigm Corporation Probe based nucleic acid detection
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
EP2764100A4 (de) * 2011-10-03 2015-09-16 Univ Florida Schnelle und zuverlässige detektion von infektionserregern
EP3718573B1 (de) 2011-12-30 2026-05-20 University of Washington through its Center for Commercialization Chromophore polymerpunkte mit schmalbandiger emission
US10648030B2 (en) 2012-01-13 2020-05-12 Affymetrix, Inc. Methods of determining the presence or absence of a plurality of target polynucleotides in a sample
EP4361606A3 (de) * 2012-02-03 2024-07-10 California Institute of Technology Signalkodierung und -dekodierung in multiplexierten biochemischen assays
CN104204228A (zh) 2012-02-14 2014-12-10 康奈尔大学 使用组合的核酸酶、连接和聚合酶反应用于核酸序列的相对定量、表达或拷贝变化的方法
CN104471077B (zh) 2012-05-21 2017-05-24 富鲁达公司 颗粒群的单颗粒分析
CN108753932B (zh) 2012-08-03 2022-12-02 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
EP2971172B1 (de) 2013-03-14 2019-07-24 Cornell University Verfahren zur relativen quantifizierung von veränderungen in einer dna-methylierung mit kombinierten nuklease-, ligations- und polymerasereaktionen
US10221447B2 (en) 2014-04-01 2019-03-05 Cornell University Detection of DNA methylation using combined nuclease ligation reactions
WO2015191777A2 (en) 2014-06-10 2015-12-17 Dxterity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
EP4026914A1 (de) 2014-10-08 2022-07-13 Cornell University Verfahren zur identifizierung und relativen quantifizierung von nukleinsäuremethylierungsänderungen unter verwendung von kombinierter nuklease, ligation und polymerasereaktionen mit verschleppungsverhinderung
US11118216B2 (en) 2015-09-08 2021-09-14 Affymetrix, Inc. Nucleic acid analysis by joining barcoded polynucleotide probes
CA3006994A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
EP3347497A4 (de) * 2016-01-31 2019-01-23 Affymetrix, Inc. Nukleinsäureanalyse durch verbinden von barcodierten polynukleotidsonden
WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
EP3781710A4 (de) 2018-04-17 2022-01-05 Chromacode, Inc. Verfahren und systeme zur multiplex-analyse
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
JP2775346B2 (ja) * 1992-04-03 1998-07-16 アプライド バイオシステムズ,インコーポレイテッド プローブ構成物および方法
WO1996015271A1 (en) 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
GB9609441D0 (en) * 1996-05-04 1996-07-10 Zeneca Ltd Process
AU730633B2 (en) * 1996-05-29 2001-03-08 Phillip Belgrader Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US7014994B1 (en) * 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6618679B2 (en) * 2000-01-28 2003-09-09 Althea Technologies, Inc. Methods for analysis of gene expression
WO2001092579A2 (en) * 2000-05-30 2001-12-06 Pe Corporation (Ny) Methods for detecting target nucleic acids using coupled ligation and amplification
US6620586B2 (en) 2001-02-20 2003-09-16 Applied Gene Technologies, Inc. Methods and compositions for analyzing nucleic acids
US20030119004A1 (en) * 2001-12-05 2003-06-26 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
EP1319718A1 (de) * 2001-12-14 2003-06-18 Keygene N.V. Hochdurchsatzanalyse und Detektion von mehreren Zielsequenzen
US20060088826A1 (en) * 2001-12-28 2006-04-27 Van Eijk Michael Josephus Ther Discrimination and detection of target nucleotide sequences using mass spectrometry
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
US7153658B2 (en) * 2002-09-19 2006-12-26 Applera Corporation Methods and compositions for detecting targets
CN1318606C (zh) * 2002-09-19 2007-05-30 阿普里拉股份有限公司 检测靶序列的方法和组合物
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US20040110134A1 (en) * 2002-12-05 2004-06-10 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
WO2005026389A2 (en) 2003-08-29 2005-03-24 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Ligation-based method of analysis of single nucleotide polymorphisms on genomic dna
EP1730312B1 (de) 2004-03-24 2008-07-02 Applera Corporation Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden

Also Published As

Publication number Publication date
JP2007530051A (ja) 2007-11-01
US7601821B2 (en) 2009-10-13
DE602005014453D1 (de) 2009-06-25
EP1730312A2 (de) 2006-12-13
WO2005098040A8 (en) 2005-12-01
US20050272071A1 (en) 2005-12-08
EP2161346A1 (de) 2010-03-10
EP1727913A2 (de) 2006-12-06
JP2007530048A (ja) 2007-11-01
ATE399884T1 (de) 2008-07-15
DE602005007874D1 (de) 2008-08-14
WO2005098040A3 (en) 2006-03-23
EP1727913B8 (de) 2009-08-19
EP1727913B1 (de) 2009-05-13
EP2161346B1 (de) 2013-06-19
WO2005094532A3 (en) 2005-12-22
WO2005094532A2 (en) 2005-10-13
WO2005098040A2 (en) 2005-10-20
EP1730312B1 (de) 2008-07-02
US7604937B2 (en) 2009-10-20
US20050260640A1 (en) 2005-11-24

Similar Documents

Publication Publication Date Title
DE602005014453D1 (de) Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
WO2006019407A3 (en) Method for detecting and quantifying rare mutations/polymorphisms
DE60133111D1 (de) Verfahren zur erkennung und zum assay von nukleinsäuresequenzen
ATE494389T1 (de) Universeller tag-assay
Lee et al. Single-molecule visualization of ROS-induced DNA damage in large DNA molecules
DE50015854D1 (de) Oligonukleotide zur schnellen Bestimmung von mikrobieller DNS/RNS
EP1442142A4 (de) Nukleinsäuresonden und verfahren zum nachweis und zur quantifizierung von nukleinsäureanalyten
WO2007002567A3 (en) Selective isolation and concentration of nucleic acids from complex samples
WO2005094532B1 (en) Encoding and decoding reactions for determining target polynucleotides
ATE368125T1 (de) Verfahren zur erkennung von einzel-nukleotid- polymorphismen
DE60229873D1 (de) Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen
JP2009106220A (ja) 基板上での等温増幅反応による標的塩基配列の捕捉及び検出方法
JP2009519001A5 (de)
KR20080084029A (ko) 앱타머를 이용한 표적 단백질 검출 방법 및 검출키트
WO2005047543A3 (en) Ligation assay
WO2007115242A3 (en) Binary probes for fluorescent analysis of nucleic acids
ATE553216T1 (de) Sonden, bibliotheken und kits zur analyse von nukleinsäuregemischen und verfahren zu deren konstruktion
DE60023539D1 (de) Immunologischer nachweis von rna:dna hybriden auf mikroarrays
ATE377091T1 (de) Verfahren zur erkennung von nukleinsäuren
GB2539675A (en) Reagents, kits and methods for molecular barcoding
TW200720441A (en) New labelling strategies for the sensitive detection of analytes
US20230417742A1 (en) Improved assays to detect nucleosome modifications using antibody-targeted enzyme digestion
ATE546550T1 (de) Verfahren zum nachweis von hpv sowie sonden, primer und kits
Wang et al. Colorimetric biosensor based on a DNAzyme primer and its application in logic gate operations for DNA screening

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties