ATE431433T1 - Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen - Google Patents

Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen

Info

Publication number
ATE431433T1
ATE431433T1 AT05760637T AT05760637T ATE431433T1 AT E431433 T1 ATE431433 T1 AT E431433T1 AT 05760637 T AT05760637 T AT 05760637T AT 05760637 T AT05760637 T AT 05760637T AT E431433 T1 ATE431433 T1 AT E431433T1
Authority
AT
Austria
Prior art keywords
present
encoding
reaction
ligation
sample
Prior art date
Application number
AT05760637T
Other languages
English (en)
Inventor
Mark R Andersen
Kenneth J Livak
Adam Broomer
Caifu Chen
Original Assignee
Applied Biosystems Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Biosystems Llc filed Critical Applied Biosystems Llc
Application granted granted Critical
Publication of ATE431433T1 publication Critical patent/ATE431433T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT05760637T 2004-03-24 2005-03-24 Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen ATE431433T1 (de)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US55622404P 2004-03-24 2004-03-24
US55616204P 2004-03-24 2004-03-24
US55615704P 2004-03-24 2004-03-24
US55616304P 2004-03-24 2004-03-24
US63068104P 2004-11-24 2004-11-24
PCT/US2005/010184 WO2005098040A2 (en) 2004-03-24 2005-03-24 Ligation and amplification reactions for determining target molecules

Publications (1)

Publication Number Publication Date
ATE431433T1 true ATE431433T1 (de) 2009-05-15

Family

ID=35064420

Family Applications (2)

Application Number Title Priority Date Filing Date
AT05760637T ATE431433T1 (de) 2004-03-24 2005-03-24 Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
AT05732398T ATE399884T1 (de) 2004-03-24 2005-03-24 Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT05732398T ATE399884T1 (de) 2004-03-24 2005-03-24 Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden

Country Status (6)

Country Link
US (2) US7601821B2 (de)
EP (3) EP1730312B1 (de)
JP (2) JP2007530051A (de)
AT (2) ATE431433T1 (de)
DE (2) DE602005014453D1 (de)
WO (2) WO2005098040A2 (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003275138A1 (en) * 2002-09-19 2004-04-08 Applied Biosystems, Llc. Methods and compositions for detecting targets
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
ATE431433T1 (de) 2004-03-24 2009-05-15 Applied Biosystems Llc Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
US7427479B2 (en) * 2004-04-30 2008-09-23 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US7364855B2 (en) * 2004-04-30 2008-04-29 Applera Corporation Methods and kits for methylation detection
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
JP2008502352A (ja) * 2004-06-30 2008-01-31 アプレラ コーポレイション ポリヌクレオチドをライゲーションするための、方法、反応混合物およびキット
US7642055B2 (en) * 2004-09-21 2010-01-05 Applied Biosystems, Llc Two-color real-time/end-point quantitation of microRNAs (miRNAs)
EP2308990B1 (de) 2005-07-15 2012-09-26 Life Technologies Corporation Analyse von Boten-RNA und Mikro-RNA im gleichen Reaktionsansatz
US20070026439A1 (en) * 2005-07-15 2007-02-01 Applera Corporation Fluid processing device and method
EP1928571B1 (de) * 2005-08-22 2013-03-13 Life Technologies Corporation Vorrichtung und verfahren zur mikrofluidischen steuerung eines ersten fluids in kontakt mit einem zweiten fluid, wobei das erste fluid und das zweite fluid nicht mischbar sind
ATE532878T1 (de) 2005-08-24 2011-11-15 Life Technologies Corp Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas
EP2677039B8 (de) * 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Erkennung von Nukleinsäuretargets mithilfe chemisch reaktiver Oligonnucleotidsonden
US8597938B2 (en) * 2007-10-12 2013-12-03 Qiagen Sciences Llc System for providing control reactions for real time RT-PCR
WO2009100449A1 (en) * 2008-02-08 2009-08-13 Fluidigm Corporation Dynamic array assay methods
WO2010027870A2 (en) * 2008-08-26 2010-03-11 Fluidigm Corporation Assay methods for increased throughput of samples and/or targets
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
JP5608998B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
AU2010232972B2 (en) * 2009-04-01 2016-10-27 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (CLPA)
EP2789694A1 (de) 2009-04-02 2014-10-15 Fluidigm Corporation Mikrofluidische Vorrichtung mit Reaktionsproduktrückgewinnungssystem
WO2011056866A2 (en) * 2009-11-05 2011-05-12 Epicentre Technologies Corporation Methods and kits for 3'-end-tagging of rna
WO2011071923A2 (en) * 2009-12-07 2011-06-16 Illumina, Inc. Multi-sample indexing for multiplex genotyping
EP2534263B1 (de) * 2010-02-09 2020-08-05 Unitaq Bio Verfahren und zusammensetzungen zur universellen erkennung von nukleinsäuren
CN103228798B (zh) 2010-09-24 2015-12-09 斯坦福大学托管董事会 使用固定的引物直接捕获、扩增及测序靶标dna
US9353406B2 (en) * 2010-10-22 2016-05-31 Fluidigm Corporation Universal probe assay methods
US20120288857A1 (en) * 2011-02-03 2012-11-15 Fluidigm Corporation Multifunctional probe-primers
US20140170653A1 (en) 2011-04-15 2014-06-19 Life Technologies Corporation Chemical ligation
US8809238B2 (en) 2011-05-09 2014-08-19 Fluidigm Corporation Probe based nucleic acid detection
EP2710145B1 (de) 2011-05-17 2015-12-09 Dxterity Diagnostics Incorporated Verfahren und zusammensetzungen zum nachweis von zielnukleinsäuren
US9074204B2 (en) 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
WO2013052124A2 (en) * 2011-10-03 2013-04-11 University Of Florida Research Foundation, Inc. Rapid and reliable detection of infectious agents
CN104487097B (zh) 2011-12-30 2019-01-18 华盛顿大学商业中心 具有窄带发射的发色聚合物点
WO2013106807A1 (en) 2012-01-13 2013-07-18 Curry John D Scalable characterization of nucleic acids by parallel sequencing
BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
SG11201404899VA (en) 2012-02-14 2014-10-30 Univ Cornell Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
JP2015519900A (ja) 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
ES2746237T3 (es) 2013-03-14 2020-03-05 Univ Cornell Método para la cuantificación relativa de cambios en la metilación del ADN, usando reacciones combinadas de nucleasa, ligamiento, y polimerasa
EP3126523B1 (de) 2014-04-01 2020-02-19 Cornell University Nachweis von dna-methylierung unter verwendung kombinierter nukleaseligationsreaktionen
EP3154696B1 (de) 2014-06-10 2020-04-08 DxTerity Diagnostics Incorporated Vorrichtungen und verfahren zur entnahme und stabilisierung biologischer proben
CA3187713A1 (en) 2014-10-08 2016-04-14 Cornell University Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention
US11118216B2 (en) 2015-09-08 2021-09-14 Affymetrix, Inc. Nucleic acid analysis by joining barcoded polynucleotide probes
US11117113B2 (en) 2015-12-16 2021-09-14 Fluidigm Corporation High-level multiplex amplification
CN108026568A (zh) * 2016-01-31 2018-05-11 阿费梅特里克斯公司 通过结合条形码标记的多核苷酸探针进行核酸分析
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
US12139734B2 (en) * 2020-05-01 2024-11-12 Helix Nanotechnologies Inc Compositions and methods for RNA synthesis

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
WO1993020236A1 (en) 1992-04-03 1993-10-14 Applied Biosystems, Inc. Probe composition and method
WO1996015271A1 (en) 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
GB9609441D0 (en) 1996-05-04 1996-07-10 Zeneca Ltd Process
JP4468488B2 (ja) 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US7014994B1 (en) * 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
EP1257664A4 (de) 2000-01-28 2006-04-05 Althea Technologies Inc Verfahren zur analyse der genexpression
WO2001092579A2 (en) * 2000-05-30 2001-12-06 Pe Corporation (Ny) Methods for detecting target nucleic acids using coupled ligation and amplification
US6620586B2 (en) 2001-02-20 2003-09-16 Applied Gene Technologies, Inc. Methods and compositions for analyzing nucleic acids
US20030119004A1 (en) 2001-12-05 2003-06-26 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
EP1319718A1 (de) * 2001-12-14 2003-06-18 Keygene N.V. Hochdurchsatzanalyse und Detektion von mehreren Zielsequenzen
US20060088826A1 (en) * 2001-12-28 2006-04-27 Van Eijk Michael Josephus Ther Discrimination and detection of target nucleotide sequences using mass spectrometry
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
EP1540003A4 (de) 2002-09-19 2006-06-21 Applera Corp Verfahren und zusammensetzung zum nachweis von targets
AU2003275138A1 (en) 2002-09-19 2004-04-08 Applied Biosystems, Llc. Methods and compositions for detecting targets
US20040235005A1 (en) 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US20040110134A1 (en) 2002-12-05 2004-06-10 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
WO2005026389A2 (en) 2003-08-29 2005-03-24 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Ligation-based method of analysis of single nucleotide polymorphisms on genomic dna
ATE431433T1 (de) 2004-03-24 2009-05-15 Applied Biosystems Llc Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen

Also Published As

Publication number Publication date
US7601821B2 (en) 2009-10-13
EP1727913B8 (de) 2009-08-19
WO2005098040A2 (en) 2005-10-20
WO2005094532A2 (en) 2005-10-13
WO2005094532A3 (en) 2005-12-22
EP1727913A2 (de) 2006-12-06
JP2007530051A (ja) 2007-11-01
US7604937B2 (en) 2009-10-20
US20050260640A1 (en) 2005-11-24
EP1730312A2 (de) 2006-12-13
ATE399884T1 (de) 2008-07-15
DE602005014453D1 (de) 2009-06-25
WO2005098040A8 (en) 2005-12-01
DE602005007874D1 (de) 2008-08-14
EP2161346B1 (de) 2013-06-19
JP2007530048A (ja) 2007-11-01
US20050272071A1 (en) 2005-12-08
EP2161346A1 (de) 2010-03-10
EP1730312B1 (de) 2008-07-02
EP1727913B1 (de) 2009-05-13
WO2005098040A3 (en) 2006-03-23

Similar Documents

Publication Publication Date Title
ATE431433T1 (de) Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
WO2006019407A3 (en) Method for detecting and quantifying rare mutations/polymorphisms
DE60133111D1 (de) Verfahren zur erkennung und zum assay von nukleinsäuresequenzen
ATE494389T1 (de) Universeller tag-assay
ATE455870T1 (de) Oligonukleotide zur schnellen bestimmung von mikrobieller dns/rns
Lee et al. Single-molecule visualization of ROS-induced DNA damage in large DNA molecules
EP1442142A4 (de) Nukleinsäuresonden und verfahren zum nachweis und zur quantifizierung von nukleinsäureanalyten
WO2007002567A3 (en) Selective isolation and concentration of nucleic acids from complex samples
WO2005094532B1 (en) Encoding and decoding reactions for determining target polynucleotides
DE60229873D1 (de) Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen
ATE506454T1 (de) Verfahren zum nachweis von cytosin-methylierung mit hoher sensitivität
JP2009519001A5 (de)
KR20080084029A (ko) 앱타머를 이용한 표적 단백질 검출 방법 및 검출키트
JP2009106220A (ja) 基板上での等温増幅反応による標的塩基配列の捕捉及び検出方法
WO2005047543A3 (en) Ligation assay
WO2007115242A3 (en) Binary probes for fluorescent analysis of nucleic acids
ATE553216T1 (de) Sonden, bibliotheken und kits zur analyse von nukleinsäuregemischen und verfahren zu deren konstruktion
DE60023539D1 (de) Immunologischer nachweis von rna:dna hybriden auf mikroarrays
WO2006128010A3 (en) Quantification of nucleic acids and proteins using oligonucleotide mass tags
DE69937447D1 (de) Verfahren zur erkennung von nukleinsäuren
TW200720441A (en) New labelling strategies for the sensitive detection of analytes
GB2539675A (en) Reagents, kits and methods for molecular barcoding
US20230417742A1 (en) Improved assays to detect nucleosome modifications using antibody-targeted enzyme digestion
ATE546550T1 (de) Verfahren zum nachweis von hpv sowie sonden, primer und kits

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties