ATE438722T1 - Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein - Google Patents

Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein

Info

Publication number
ATE438722T1
ATE438722T1 AT01930179T AT01930179T ATE438722T1 AT E438722 T1 ATE438722 T1 AT E438722T1 AT 01930179 T AT01930179 T AT 01930179T AT 01930179 T AT01930179 T AT 01930179T AT E438722 T1 ATE438722 T1 AT E438722T1
Authority
AT
Austria
Prior art keywords
sugar chain
reduction
protein
mannose phosphate
glycoprotein
Prior art date
Application number
AT01930179T
Other languages
English (en)
Inventor
Masami Miura
Masaaki Hirose
Taeko Miwa
Hiroyuki Irie
Shinobu Kuwae
Kenmi Miyano
Wataru Otani
Hideyuki Ohi
Original Assignee
Mitsubishi Tanabe Pharma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Tanabe Pharma Corp filed Critical Mitsubishi Tanabe Pharma Corp
Application granted granted Critical
Publication of ATE438722T1 publication Critical patent/ATE438722T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT01930179T 2000-05-17 2001-05-16 Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein ATE438722T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000144643 2000-05-17
PCT/JP2001/004070 WO2001088143A1 (en) 2000-05-17 2001-05-16 Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby

Publications (1)

Publication Number Publication Date
ATE438722T1 true ATE438722T1 (de) 2009-08-15

Family

ID=18651270

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01930179T ATE438722T1 (de) 2000-05-17 2001-05-16 Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein

Country Status (9)

Country Link
US (3) US7198921B2 (de)
EP (1) EP1283265B1 (de)
JP (2) JP4969011B2 (de)
AT (1) ATE438722T1 (de)
AU (1) AU2001256762A1 (de)
DE (1) DE60139467D1 (de)
DK (1) DK1283265T3 (de)
ES (1) ES2328106T3 (de)
WO (1) WO2001088143A1 (de)

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WO2001088143A1 (en) * 2000-05-17 2001-11-22 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
CN100385006C (zh) 2001-01-19 2008-04-30 陶氏化学公司 利用植物细胞分泌性生产具有人型糖链的糖蛋白的方法
EP1696864B1 (de) * 2003-12-24 2014-11-12 GlycoFi, Inc. Verfahren zur elimination der mannosylphosphorylierung von glykanen bei der herstellung von glykoproteinen
JP4954866B2 (ja) 2004-04-29 2012-06-20 グライコフィ， インコーポレイテッド 糖タンパク質の作製においてアルファ−マンノシダーゼ抵抗性グリカンを減少させるか又は排除する方法
US8202523B2 (en) * 2005-09-22 2012-06-19 ProSci, Inc. Glycosylated polypeptides produced in yeast mutants and methods of use thereof
KR20090130029A (ko) * 2007-03-07 2009-12-17 글리코파이, 인크. 푸코실화가 변형된 당단백질의 제조
JP5967861B2 (ja) * 2007-04-03 2016-08-10 オキシレイン ユーケー リミテッド 分子のグリコシル化
KR101426667B1 (ko) 2007-04-17 2014-08-06 스티칭 디엔스트 랜드보위쿤디그 온데조에크 비-포유동물 글리코실트랜스퍼라제의 발현에 의한 식물에서의 포유동물형 글리코실화
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
EP2235198B1 (de) 2007-12-19 2018-08-29 GlycoFi, Inc. Hefestämme für die Proteinproduktion
JP2011514157A (ja) 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
CA2715212A1 (en) 2008-03-03 2009-09-11 Glycofi, Inc. Surface display of recombinant proteins in lower eukaryotes
US8067339B2 (en) * 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
WO2010019487A1 (en) 2008-08-12 2010-02-18 Glycofi, Inc. Improved vectors and yeast strains for protein production: ca2+ atpase overexpression
EP2401378B1 (de) 2009-02-25 2013-08-14 Merck Sharp & Dohme Corp. Stoffwechselmanipulation eines galaktoseassimilationswegs bei der glycoengineerten hefe pichia pastoris
KR102000383B1 (ko) 2009-09-29 2019-07-15 유니버시테이트 젠트 만노스-1-포스포-6-만노스 결합의 포스포-6-만노스로의 가수분해
JP2013507921A (ja) 2009-10-16 2013-03-07 メルク・シャープ・エンド・ドーム・コーポレイション 宿主細胞抗原に対する抗体に対する検出可能な交差結合活性を欠くタンパク質の、ピチア・パストリスにおける製造方法
JP2013509180A (ja) 2009-10-30 2013-03-14 メルク・シャープ・エンド・ドーム・コーポレイション 改善された分泌効率を有する組換えタンパク質の産生方法
CN102725396A (zh) 2009-10-30 2012-10-10 默沙东公司 在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法
US20130053550A1 (en) 2009-11-19 2013-02-28 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
BR112012020882A2 (pt) 2010-02-24 2015-11-03 Merck Sharp & Dohme métodos para produzir uma glicoproteína heteróloga e para produzir uma composição de glicoproteína, célula hospedeira, composição de glicoproteína, e, uso da pichia pastoris recombinante.
AU2011258425B2 (en) 2010-05-27 2015-12-10 Merck Sharp & Dohme Llc Method for preparing antibodies having improved properties
BR112013007616B1 (pt) 2010-09-29 2023-01-24 Oxyrane Uk Limited Métodos para retirar o cap ("uncapping") de porções de manose-1- fosfo-6-manose e desmanosilar n-glicanos fosforilados em uma glicoproteína, para direcionar uma glicoproteína para o interior de uma célula de mamífero, e para converter uma glicoproteína de uma primeira forma que não se liga substancialmente a um receptor de manose-6-fosfato em uma segunda forma que se liga substancialmente a um receptor de manose-6-fosfato em uma célula de mamífero
JP6131190B2 (ja) 2010-09-29 2017-05-17 オキシレイン ユーケー リミテッド リン酸化ｎ−グリカンの脱マンノシル化
US9365846B2 (en) 2010-12-01 2016-06-14 Merck Sharp & Dohme Corp. Surface, anchored Fc-bait antibody display system
WO2012115903A2 (en) 2011-02-25 2012-08-30 Merck Sharp & Dohme Corp. Yeast strain for the production of proteins with modified o-glycosylation
AU2012258907A1 (en) 2011-05-25 2013-11-07 Merck Sharp & Dohme Corp. Method for preparing Fc-containing polypeptides having improved properties
WO2013062940A1 (en) 2011-10-28 2013-05-02 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression
CA2867237C (en) 2012-03-15 2024-01-02 Wouter Vervecken Methods and materials for treatment of pompe's disease
CN104271816A (zh) 2012-05-11 2015-01-07 默沙东公司 表面锚定的轻链诱饵抗体展示系统
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
AU2013335065B2 (en) 2012-10-22 2018-10-25 Merck Sharp & Dohme Llc CRZ1 mutant fungal cells
EP2925345B1 (de) 2012-12-03 2018-09-05 Merck Sharp & Dohme Corp. Methode zur herstellung von o-glycosyliertem carboxyterminus-peptid (ctp)-basiertem insulin und insulinanaloga
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi

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IL82935A0 (en) 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
JPH093097A (ja) 1995-06-16 1997-01-07 Green Cross Corp:The ピキア属酵母由来の糖鎖伸長タンパク、該蛋白質をコードするｄｎａ、修飾該ｄｎａおよび形質転換体
JP2770010B2 (ja) * 1996-03-29 1998-06-25 工業技術院長 酵母のマンノース−１−リン酸転移を正に制御する遺伝子ならびにこの遺伝子の欠損変異株を利用する高マンノース型中性糖鎖の製造方法
US6747137B1 (en) * 1998-02-13 2004-06-08 Genome Therapeutics Corporation Nucleic acid sequences relating to Candida albicans for diagnostics and therapeutics
WO2001088143A1 (en) 2000-05-17 2001-11-22 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins

Also Published As

Publication number Publication date
US7488591B2 (en) 2009-02-10
DK1283265T3 (da) 2009-11-09
US7872111B2 (en) 2011-01-18
EP1283265A1 (de) 2003-02-12
WO2001088143A1 (en) 2001-11-22
AU2001256762A1 (en) 2001-11-26
EP1283265B1 (de) 2009-08-05
JP2012105652A (ja) 2012-06-07
US20040014170A1 (en) 2004-01-22
US20090004725A1 (en) 2009-01-01
US20070202569A1 (en) 2007-08-30
DE60139467D1 (de) 2009-09-17
JP4969011B2 (ja) 2012-07-04
ES2328106T3 (es) 2009-11-10
EP1283265A4 (de) 2004-10-20
US7198921B2 (en) 2007-04-03

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