ATE440965T1 - Neue zusammensetzung und methode zur 'hot start' nukleinsäureamplifizierung - Google Patents
Neue zusammensetzung und methode zur 'hot start' nukleinsäureamplifizierungInfo
- Publication number
- ATE440965T1 ATE440965T1 AT02015234T AT02015234T ATE440965T1 AT E440965 T1 ATE440965 T1 AT E440965T1 AT 02015234 T AT02015234 T AT 02015234T AT 02015234 T AT02015234 T AT 02015234T AT E440965 T1 ATE440965 T1 AT E440965T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- acid amplification
- new composition
- hot start
- composition
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 2
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01115788A EP1275735A1 (de) | 2001-07-11 | 2001-07-11 | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE440965T1 true ATE440965T1 (de) | 2009-09-15 |
Family
ID=8177866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02015234T ATE440965T1 (de) | 2001-07-11 | 2002-07-09 | Neue zusammensetzung und methode zur 'hot start' nukleinsäureamplifizierung |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7122355B2 (de) |
| EP (1) | EP1275735A1 (de) |
| JP (1) | JP4053363B2 (de) |
| AT (1) | ATE440965T1 (de) |
| DE (1) | DE60233462D1 (de) |
| ES (1) | ES2331112T3 (de) |
Families Citing this family (67)
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| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| CA2476481C (en) | 2002-02-21 | 2016-01-26 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| CA2472554A1 (en) * | 2002-02-27 | 2003-09-04 | Invitrogen Corporation | Methods of using fet labeled oligonucleotides that include a 3' 5' exonuclease resistant quencher domain and compositions for practicing the same |
| US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
| CA2486283A1 (en) * | 2002-05-17 | 2004-02-05 | Nugen Technologies, Inc. | Methods for fragmentation, labeling and immobilization of nucleic acids |
| DE10315640A1 (de) * | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
| EP1502958A1 (de) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion |
| US8304189B2 (en) | 2003-12-01 | 2012-11-06 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
| CA2552007A1 (en) * | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
| US7445898B2 (en) | 2004-04-01 | 2008-11-04 | Bio-Rad Laboratories, Inc. | Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity |
| US8158388B2 (en) * | 2004-10-21 | 2012-04-17 | New England Biolabs, Inc. | Repair of nucleic acids for improved amplification |
| US7700283B2 (en) * | 2004-10-21 | 2010-04-20 | New England Biolabs, Inc. | Repair of nucleic acids for improved amplification |
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| EP1929049B1 (de) * | 2005-07-25 | 2013-04-10 | Alere San Diego, Inc. | Verfahren zur multiplexierung einer rekombinase-polymerase-verstärkung |
| EP1915446B1 (de) | 2005-08-11 | 2017-06-14 | Synthetic Genomics, Inc. | In vitro-rekombinationsmethode |
| DE102005046348B4 (de) * | 2005-09-15 | 2008-08-07 | Universität Leipzig | Thermostabiles Enzym mit 3'-5'-Exonuklease-Aktivität |
| CN103172700B (zh) | 2006-02-27 | 2014-12-17 | 霍夫曼-拉罗奇有限公司 | 经由镁螯合的pcr热启动 |
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| US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
| EP2038429B1 (de) * | 2006-06-30 | 2013-08-21 | Nugen Technologies, Inc. | Verfahren zur fragmentierung und etikettierung von nukleinsäuren |
| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
| CN101421420B (zh) * | 2006-08-08 | 2015-03-11 | 爱科来株式会社 | 突变的检测方法及用于该方法的试剂盒 |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
| EP2255013B1 (de) * | 2008-02-15 | 2016-06-08 | Synthetic Genomics, Inc. | Verfahren zur in-vitro-zusammenfügung und kombinatorischen konstruktion von nukleinsäuremolekülen |
| CA2658520C (en) | 2008-03-19 | 2016-11-08 | F. Hoffmann-La Roche Ag | Nucleic acid amplification in the presence of modified randomers |
| EP2644710B1 (de) | 2008-04-30 | 2016-12-21 | Integrated Dna Technologies, Inc. | Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US7910720B2 (en) | 2008-09-09 | 2011-03-22 | Roche Diagnostics Operations, Inc. | Polyanion for improved nucleic acid amplification |
| EP2382321A4 (de) | 2009-01-08 | 2012-12-19 | Bio Rad Laboratories | Verfahren und zusammensetzungen zur verbesserung der wirksamkeit bei einer nukleinsäureverstärkungsreaktionen |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| US9347092B2 (en) | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
| US9469867B2 (en) | 2009-05-20 | 2016-10-18 | Alere San Diego, Inc. | DNA glycosylase/lyase and AP endonuclease substrates |
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| KR20210072137A (ko) | 2012-06-14 | 2021-06-16 | 라이프 테크놀로지스 코포레이션 | 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트 |
| JP6181751B2 (ja) | 2012-06-18 | 2017-08-16 | ニューゲン テクノロジーズ， インコーポレイテッド | 望まれない核酸配列のネガティブ選択のための組成物および方法 |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
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| CA2929596C (en) | 2013-11-13 | 2022-07-05 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
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| ES2832748T3 (es) | 2014-08-19 | 2021-06-11 | Hoffmann La Roche | Procedimientos y composiciones para la detección de ácidos nucleicos |
| CN114507715B (zh) * | 2014-11-20 | 2025-01-07 | 杭州智灵龙生物科技有限公司 | 用于核酸扩增的组合物和方法 |
| WO2017121836A1 (en) | 2016-01-15 | 2017-07-20 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
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| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
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| CN110699426B (zh) | 2019-01-02 | 2022-01-28 | 上海臻迪基因科技有限公司 | 基因目标区域富集方法及试剂盒 |
| JP7687957B2 (ja) | 2019-03-14 | 2025-06-03 | インシリクサ， インコーポレイテッド | 時間ゲート蛍光ベースの検出のための方法およびシステム |
| EP3980552A4 (de) * | 2019-06-06 | 2023-06-21 | The Regents Of The University Of Colorado, A Body Corporate | Neuartige systeme, verfahren und zusammensetzungen zur direkten synthese von polynukleotiden mit überhängenden enden |
| EP3851542A1 (de) | 2020-01-20 | 2021-07-21 | Tecan Genomics, Inc. | Verarmung von reichlich uninformativen sequenzen |
| CN113275053A (zh) | 2020-02-03 | 2021-08-20 | 帝肯基因组学公司 | 试剂存储系统 |
| US20250051737A1 (en) * | 2021-12-24 | 2025-02-13 | Bgi Shenzhen | Dna polymerase mutant and use thereof |
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| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US4889818A (en) * | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
| US5118801A (en) * | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| DE69118930T2 (de) | 1990-01-26 | 1997-01-09 | Abbott Lab | Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion |
| US5756334A (en) | 1990-04-26 | 1998-05-26 | New England Biolabs, Inc. | Thermostable DNA polymerase from 9°N-7 and methods for producing the same |
| US5352778A (en) * | 1990-04-26 | 1994-10-04 | New England Biolabs, Inc. | Recombinant thermostable DNA polymerase from archaebacteria |
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| US6482590B1 (en) * | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
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| ATE286981T1 (de) * | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
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-
2001
- 2001-07-11 EP EP01115788A patent/EP1275735A1/de not_active Withdrawn
-
2002
- 2002-07-09 DE DE60233462T patent/DE60233462D1/de not_active Expired - Lifetime
- 2002-07-09 AT AT02015234T patent/ATE440965T1/de not_active IP Right Cessation
- 2002-07-09 ES ES02015234T patent/ES2331112T3/es not_active Expired - Lifetime
- 2002-07-11 US US10/192,902 patent/US7122355B2/en not_active Expired - Lifetime
- 2002-07-11 JP JP2002202533A patent/JP4053363B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE60233462D1 (de) | 2009-10-08 |
| JP2003038180A (ja) | 2003-02-12 |
| US7122355B2 (en) | 2006-10-17 |
| ES2331112T3 (es) | 2009-12-22 |
| EP1275735A1 (de) | 2003-01-15 |
| JP4053363B2 (ja) | 2008-02-27 |
| US20030119150A1 (en) | 2003-06-26 |
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