ATE440965T1 - Neue zusammensetzung und methode zur 'hot start' nukleinsäureamplifizierung - Google Patents
Neue zusammensetzung und methode zur 'hot start' nukleinsäureamplifizierungInfo
- Publication number
- ATE440965T1 ATE440965T1 AT02015234T AT02015234T ATE440965T1 AT E440965 T1 ATE440965 T1 AT E440965T1 AT 02015234 T AT02015234 T AT 02015234T AT 02015234 T AT02015234 T AT 02015234T AT E440965 T1 ATE440965 T1 AT E440965T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- acid amplification
- new composition
- hot start
- composition
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 2
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01115788A EP1275735A1 (de) | 2001-07-11 | 2001-07-11 | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE440965T1 true ATE440965T1 (de) | 2009-09-15 |
Family
ID=8177866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02015234T ATE440965T1 (de) | 2001-07-11 | 2002-07-09 | Neue zusammensetzung und methode zur 'hot start' nukleinsäureamplifizierung |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7122355B2 (de) |
| EP (1) | EP1275735A1 (de) |
| JP (1) | JP4053363B2 (de) |
| AT (1) | ATE440965T1 (de) |
| DE (1) | DE60233462D1 (de) |
| ES (1) | ES2331112T3 (de) |
Families Citing this family (67)
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| NZ512244A (en) | 1998-11-12 | 2003-12-19 | Invitrogen Corp | Polycationic transfection reagents for introducing anions into a cell |
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| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| US7270981B2 (en) | 2002-02-21 | 2007-09-18 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| JP4481656B2 (ja) * | 2002-02-27 | 2010-06-16 | ライフ テクノロジーズ コーポレーション | ３’→５’エキソヌクレアーゼ耐性クエンチャードメインを含むｆｅｔ標識オリゴヌクレオチドを使用する方法およびそれを実施するための組成物 |
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| AU2003279697A1 (en) * | 2002-05-17 | 2004-02-16 | Nugen Technologies, Inc. | Methods for fragmentation, labeling and immobilizaton of nucleic acids |
| DE10315640A1 (de) * | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
| EP1502958A1 (de) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion |
| WO2005054438A2 (en) | 2003-12-01 | 2005-06-16 | Invitrogen Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
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| US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
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| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
| EP2031074A4 (de) * | 2006-08-08 | 2010-09-08 | Arkray Inc | Verfahren für variationsnachweis und kit dafür |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
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| CA2658520C (en) | 2008-03-19 | 2016-11-08 | F. Hoffmann-La Roche Ag | Nucleic acid amplification in the presence of modified randomers |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| EP2644708B1 (de) | 2008-04-30 | 2014-12-17 | Integrated Dna Technologies, Inc. | Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren |
| US7910720B2 (en) | 2008-09-09 | 2011-03-22 | Roche Diagnostics Operations, Inc. | Polyanion for improved nucleic acid amplification |
| JP5818688B2 (ja) | 2009-01-08 | 2015-11-18 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | 核酸増幅反応の効率を改善するための方法および組成物 |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| US9347092B2 (en) | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
| JP5847076B2 (ja) | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質 |
| CN102549154A (zh) * | 2009-07-17 | 2012-07-04 | 东洋制罐株式会社 | 核酸扩增方法 |
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| EP2807292B1 (de) | 2012-01-26 | 2019-05-22 | Tecan Genomics, Inc. | Zusammensetzungen und verfahren zur gezielten nukleinsäuresequenzanreicherung und erzeugung einer hocheffizienten bibliothek |
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| JP6450308B2 (ja) | 2012-06-14 | 2019-01-09 | ライフ テクノロジーズ コーポレーション | ポリメラーゼ連鎖反応（ｐｃｒ）のための新規の組成物、方法、およびキット |
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| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
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| WO2015073711A1 (en) | 2013-11-13 | 2015-05-21 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| EP3169310A1 (de) | 2014-07-15 | 2017-05-24 | Life Technologies Corporation | Zusammensetzungen mit lipidaggregaten und verfahren zur effizienten bereitstellung von molekülen an zellen |
| CN107109474B (zh) | 2014-08-19 | 2021-03-19 | 豪夫迈·罗氏有限公司 | 用于核酸检测的方法和组合物 |
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| WO2017121836A1 (en) | 2016-01-15 | 2017-07-20 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
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| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| EP3645710A1 (de) | 2017-06-26 | 2020-05-06 | Thermo Fisher Scientific Baltics Uab | Thermophile dna-polymerase-mutanten |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| CN110699426B (zh) | 2019-01-02 | 2022-01-28 | 上海臻迪基因科技有限公司 | 基因目标区域富集方法及试剂盒 |
| JP7687957B2 (ja) | 2019-03-14 | 2025-06-03 | インシリクサ， インコーポレイテッド | 時間ゲート蛍光ベースの検出のための方法およびシステム |
| JP7684694B2 (ja) * | 2019-06-06 | 2025-05-28 | ザ・リージエンツ・オブ・ザ・ユニバーシテイ・オブ・コロラド、ア・ボデイー・コーポレイト | 粘着末端型ポリヌクレオチドの直接合成のための新規のシステム、方法、および組成物 |
| EP3851542A1 (de) | 2020-01-20 | 2021-07-21 | Tecan Genomics, Inc. | Verarmung von reichlich uninformativen sequenzen |
| US12059674B2 (en) | 2020-02-03 | 2024-08-13 | Tecan Genomics, Inc. | Reagent storage system |
| US20250051737A1 (en) * | 2021-12-24 | 2025-02-13 | Bgi Shenzhen | Dna polymerase mutant and use thereof |
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| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
| US4889818A (en) * | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5118801A (en) * | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| CA2035010C (en) | 1990-01-26 | 1996-12-10 | Keith C. Backman | Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
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| US5352778A (en) * | 1990-04-26 | 1994-10-04 | New England Biolabs, Inc. | Recombinant thermostable DNA polymerase from archaebacteria |
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| ATE286981T1 (de) * | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
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-
2001
- 2001-07-11 EP EP01115788A patent/EP1275735A1/de not_active Withdrawn
-
2002
- 2002-07-09 AT AT02015234T patent/ATE440965T1/de not_active IP Right Cessation
- 2002-07-09 DE DE60233462T patent/DE60233462D1/de not_active Expired - Lifetime
- 2002-07-09 ES ES02015234T patent/ES2331112T3/es not_active Expired - Lifetime
- 2002-07-11 US US10/192,902 patent/US7122355B2/en not_active Expired - Lifetime
- 2002-07-11 JP JP2002202533A patent/JP4053363B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2003038180A (ja) | 2003-02-12 |
| EP1275735A1 (de) | 2003-01-15 |
| JP4053363B2 (ja) | 2008-02-27 |
| DE60233462D1 (de) | 2009-10-08 |
| ES2331112T3 (es) | 2009-12-22 |
| US7122355B2 (en) | 2006-10-17 |
| US20030119150A1 (en) | 2003-06-26 |
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