ATE440965T1 - Neue zusammensetzung und methode zur 'hot start' nukleinsäureamplifizierung - Google Patents
Neue zusammensetzung und methode zur 'hot start' nukleinsäureamplifizierungInfo
- Publication number
- ATE440965T1 ATE440965T1 AT02015234T AT02015234T ATE440965T1 AT E440965 T1 ATE440965 T1 AT E440965T1 AT 02015234 T AT02015234 T AT 02015234T AT 02015234 T AT02015234 T AT 02015234T AT E440965 T1 ATE440965 T1 AT E440965T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- acid amplification
- new composition
- hot start
- composition
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 2
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01115788A EP1275735A1 (de) | 2001-07-11 | 2001-07-11 | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE440965T1 true ATE440965T1 (de) | 2009-09-15 |
Family
ID=8177866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02015234T ATE440965T1 (de) | 2001-07-11 | 2002-07-09 | Neue zusammensetzung und methode zur 'hot start' nukleinsäureamplifizierung |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7122355B2 (de) |
| EP (1) | EP1275735A1 (de) |
| JP (1) | JP4053363B2 (de) |
| AT (1) | ATE440965T1 (de) |
| DE (1) | DE60233462D1 (de) |
| ES (1) | ES2331112T3 (de) |
Families Citing this family (67)
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| CA2350882C (en) | 1998-11-12 | 2011-02-22 | Life Technologies Corporation | Transfection reagents |
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| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| DK1499738T3 (da) | 2002-02-21 | 2008-11-17 | Asm Scient Inc | Recombinase-polymerease-amplifikation |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
| JP4481656B2 (ja) * | 2002-02-27 | 2010-06-16 | ライフ テクノロジーズ コーポレーション | ３’→５’エキソヌクレアーゼ耐性クエンチャードメインを含むｆｅｔ標識オリゴヌクレオチドを使用する方法およびそれを実施するための組成物 |
| WO2004011665A2 (en) * | 2002-05-17 | 2004-02-05 | Nugen Technologies, Inc. | Methods for fragmentation, labeling and immobilization of nucleic acids |
| DE10315640A1 (de) * | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
| EP1502958A1 (de) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion |
| DE602004027538D1 (de) | 2003-12-01 | 2010-07-15 | Life Technologies Corp | Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon |
| CA2552007A1 (en) * | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
| EP1740711A2 (de) | 2004-04-01 | 2007-01-10 | Bio-Rad Laboratories, Inc. | Quantitative amplifikation mit einer markierten sonde und 3' nach 5' exonukleaseaktivität |
| KR20070073917A (ko) * | 2004-10-21 | 2007-07-10 | 뉴 잉글랜드 바이오랩스, 인크 | 개선된 증폭을 위한 핵산 복구 |
| US8158388B2 (en) * | 2004-10-21 | 2012-04-17 | New England Biolabs, Inc. | Repair of nucleic acids for improved amplification |
| AU2006269282B2 (en) * | 2005-07-07 | 2013-10-24 | Quanta Biosciences, Inc. | Compositions and methods for increasing amplification efficiency |
| EP2829615B1 (de) * | 2005-07-25 | 2018-05-09 | Alere San Diego, Inc. | Kit zur Multiplexierung einer Rekombinase-Polymerase-Verstärkung |
| DK1915446T3 (en) | 2005-08-11 | 2017-09-11 | Synthetic Genomics Inc | IN VITRO RECOMBINATION PROCEDURE |
| DE102005046348B4 (de) * | 2005-09-15 | 2008-08-07 | Universität Leipzig | Thermostabiles Enzym mit 3'-5'-Exonuklease-Aktivität |
| CN101421417B (zh) | 2006-02-27 | 2013-04-03 | 霍夫曼-拉罗奇有限公司 | 经由镁螯合的pcr热启动 |
| US20100173364A1 (en) * | 2006-04-11 | 2010-07-08 | New England Biolabs, Inc. | Repair of Nucleic Acids for Improved Amplification |
| EP2029782B1 (de) | 2006-05-04 | 2014-11-26 | Alere San Diego, Inc. | Rekombinase-polymerase-verstärkung |
| JP5558811B2 (ja) | 2006-06-01 | 2014-07-23 | トリリンク バイオテクノロジーズ | 核酸増幅のための化学的に修飾されたオリゴヌクレオチドプライマー |
| US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
| WO2008005459A2 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| WO2008014485A2 (en) | 2006-07-28 | 2008-01-31 | California Institute Of Technology | Multiplex q-pcr arrays |
| KR101171635B1 (ko) * | 2006-08-08 | 2012-08-09 | 아크레이 가부시키가이샤 | 변이의 검출 방법 및 그것에 이용하는 키트 |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
| EP3064599B1 (de) * | 2008-02-15 | 2018-12-12 | Synthetic Genomics, Inc. | Verfahren zur in-vitro-verbindung und zur kombinatorischen anordnung von nukleinsäuremolekülen |
| CA2658520C (en) | 2008-03-19 | 2016-11-08 | F. Hoffmann-La Roche Ag | Nucleic acid amplification in the presence of modified randomers |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| EP3150727B1 (de) | 2008-04-30 | 2019-07-10 | Integrated DNA Technologies Inc. | Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren |
| US9434988B2 (en) * | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US7910720B2 (en) | 2008-09-09 | 2011-03-22 | Roche Diagnostics Operations, Inc. | Polyanion for improved nucleic acid amplification |
| JP5818688B2 (ja) | 2009-01-08 | 2015-11-18 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | 核酸増幅反応の効率を改善するための方法および組成物 |
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| EP2488664A4 (de) * | 2009-10-13 | 2014-04-09 | Syntezza Molecular Detection Israel Ltd | Verfahren und zusammensetzungen zur verstärkung von zielsequenzen aus nukleinsäureproben |
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| US8680017B2 (en) * | 2010-02-13 | 2014-03-25 | Gregory Francis Lopreato | Lariat aptamer: aptamer candidate exclusion by nuclease digestion |
| EP2769007B1 (de) | 2011-10-19 | 2016-12-07 | Nugen Technologies, Inc. | Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren |
| CN105861487B (zh) | 2012-01-26 | 2020-05-05 | 纽亘技术公司 | 用于靶向核酸序列富集和高效文库产生的组合物和方法 |
| SG10201802883UA (en) * | 2012-04-19 | 2018-05-30 | Life Technologies Corp | Nucleic acid amplification |
| US9951378B2 (en) | 2012-06-14 | 2018-04-24 | Life Technologies Corporation | Compositions, methods and kits for real time polymerase chain reaction (PCR) |
| CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| EP2971130A4 (de) | 2013-03-15 | 2016-10-05 | Nugen Technologies Inc | Sequentielle sequenzierung |
| JP6525473B2 (ja) | 2013-11-13 | 2019-06-05 | ニューゲン テクノロジーズ， インコーポレイテッド | 複製物配列決定リードを同定するための組成物および方法 |
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| US10195280B2 (en) | 2014-07-15 | 2019-02-05 | Life Technologies Corporation | Compositions and methods for efficient delivery of molecules to cells |
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| CN107208143A (zh) * | 2014-11-20 | 2017-09-26 | 安普里怀斯公司 | 用于核酸扩增和分析的组合物、方法、系统和试剂盒 |
| LT3402880T (lt) | 2016-01-15 | 2024-03-12 | Thermo Fisher Scientific Baltics Uab | Termofiliniai dnr polimerazės mutantai |
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| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| CN110699426B (zh) | 2019-01-02 | 2022-01-28 | 上海臻迪基因科技有限公司 | 基因目标区域富集方法及试剂盒 |
| EP3937780A4 (de) | 2019-03-14 | 2022-12-07 | InSilixa, Inc. | Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion |
| EP3980552A4 (de) * | 2019-06-06 | 2023-06-21 | The Regents Of The University Of Colorado, A Body Corporate | Neuartige systeme, verfahren und zusammensetzungen zur direkten synthese von polynukleotiden mit überhängenden enden |
| EP3851542A1 (de) | 2020-01-20 | 2021-07-21 | Tecan Genomics, Inc. | Verarmung von reichlich uninformativen sequenzen |
| CN113275053A (zh) | 2020-02-03 | 2021-08-20 | 帝肯基因组学公司 | 试剂存储系统 |
| CN118339283A (zh) * | 2021-12-24 | 2024-07-12 | 深圳华大生命科学研究院 | Dna聚合酶突变体及其应用 |
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| CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
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| US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| KR950013953B1 (ko) | 1990-01-26 | 1995-11-18 | 애보트 래보라토리즈 | 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법 |
| US5322785A (en) | 1990-04-26 | 1994-06-21 | New England Biolabs, Inc. | Purified thermostable DNA polymerase obtainable from thermococcus litoralis |
| US5352778A (en) | 1990-04-26 | 1994-10-04 | New England Biolabs, Inc. | Recombinant thermostable DNA polymerase from archaebacteria |
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| DE69725076T2 (de) * | 1996-07-29 | 2004-04-15 | Toyo Boseki K.K. | Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren |
| EP0834571A1 (de) | 1996-10-03 | 1998-04-08 | Roche Diagnostics GmbH | Thermostabile DNS Polymerase aus Thermococcus Gorgonarius |
| US6482590B1 (en) * | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
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| ATE286981T1 (de) * | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
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-
2001
- 2001-07-11 EP EP01115788A patent/EP1275735A1/de not_active Withdrawn
-
2002
- 2002-07-09 DE DE60233462T patent/DE60233462D1/de not_active Expired - Lifetime
- 2002-07-09 AT AT02015234T patent/ATE440965T1/de not_active IP Right Cessation
- 2002-07-09 ES ES02015234T patent/ES2331112T3/es not_active Expired - Lifetime
- 2002-07-11 JP JP2002202533A patent/JP4053363B2/ja not_active Expired - Lifetime
- 2002-07-11 US US10/192,902 patent/US7122355B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US7122355B2 (en) | 2006-10-17 |
| ES2331112T3 (es) | 2009-12-22 |
| JP2003038180A (ja) | 2003-02-12 |
| JP4053363B2 (ja) | 2008-02-27 |
| EP1275735A1 (de) | 2003-01-15 |
| US20030119150A1 (en) | 2003-06-26 |
| DE60233462D1 (de) | 2009-10-08 |
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