ATE427361T1 - Verfahren zur dna-amplifikation und - sequenzierung - Google Patents
Verfahren zur dna-amplifikation und - sequenzierungInfo
- Publication number
- ATE427361T1 ATE427361T1 AT99903891T AT99903891T ATE427361T1 AT E427361 T1 ATE427361 T1 AT E427361T1 AT 99903891 T AT99903891 T AT 99903891T AT 99903891 T AT99903891 T AT 99903891T AT E427361 T1 ATE427361 T1 AT E427361T1
- Authority
- AT
- Austria
- Prior art keywords
- thp
- dna
- sequencing
- tetrahydropyrimidine
- proline
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 230000004544 DNA amplification Effects 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 abstract 2
- 238000002844 melting Methods 0.000 abstract 2
- 230000008018 melting Effects 0.000 abstract 2
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- WQXNXVUDBPYKBA-UHFFFAOYSA-N 2-Methyl-4-carboxy-3,4,5,6-tetrahydropyrimidine Chemical compound CC1=NCCC(C(O)=O)N1 WQXNXVUDBPYKBA-UHFFFAOYSA-N 0.000 abstract 1
- KIIBBJKLKFTNQO-UHFFFAOYSA-N 5-hydroxy-2-methyl-1,4,5,6-tetrahydropyrimidin-3-ium-6-carboxylate Chemical compound CC1=NCC(O)C(C(O)=O)N1 KIIBBJKLKFTNQO-UHFFFAOYSA-N 0.000 abstract 1
- 238000001712 DNA sequencing Methods 0.000 abstract 1
- 230000006820 DNA synthesis Effects 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL12325698A IL123256A0 (en) | 1998-02-10 | 1998-02-10 | Methods for dna amplification and sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE427361T1 true ATE427361T1 (de) | 2009-04-15 |
Family
ID=11071218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99903891T ATE427361T1 (de) | 1998-02-10 | 1999-02-08 | Verfahren zur dna-amplifikation und - sequenzierung |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US6428986B1 (de) |
| EP (1) | EP1053350B1 (de) |
| JP (1) | JP4393700B2 (de) |
| AT (1) | ATE427361T1 (de) |
| DE (1) | DE69940662D1 (de) |
| ES (1) | ES2323561T3 (de) |
| IL (1) | IL123256A0 (de) |
| WO (1) | WO1999041410A1 (de) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL123256A0 (en) * | 1998-02-10 | 1998-09-24 | Yeda Res & Dev | Methods for dna amplification and sequencing |
| US6787305B1 (en) | 1998-03-13 | 2004-09-07 | Invitrogen Corporation | Compositions and methods for enhanced synthesis of nucleic acid molecules |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| ES2296419T3 (es) | 1998-11-12 | 2008-04-16 | Invitrogen Corporation | Reactivos de transfeccion. |
| US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US7101111B2 (en) * | 1999-07-19 | 2006-09-05 | Exodyne Technologies Inc. | Flared energy absorbing system and method |
| US7306397B2 (en) * | 2002-07-22 | 2007-12-11 | Exodyne Technologies, Inc. | Energy attenuating safety system |
| WO2001023618A2 (en) * | 1999-09-30 | 2001-04-05 | Qiagen Genomics, Inc. | Compositions and methods for reducing oligonucleotide hybridization and priming specificity |
| CN1326990A (zh) * | 2000-06-07 | 2001-12-19 | 上海博德基因开发有限公司 | 一种新的多肽——人类dna cgg重复结合蛋白16.17和编码这种多肽的多核苷酸 |
| EP1368497A4 (de) | 2001-03-12 | 2007-08-15 | California Inst Of Techn | Verfahren und vorrichtung zur analyse von polynukleotidsequenzen durch asynchrone basenverlängerung |
| AU2003272174A1 (en) * | 2002-10-21 | 2004-05-04 | Biotage Ab | Use of osmolytes to heat stabilise luciferase and/or apyrase |
| EP1594975A4 (de) | 2002-12-04 | 2006-08-02 | Applera Corp | Multiplex-amplifikation von polynukleotiden |
| US20060193688A1 (en) * | 2003-03-05 | 2006-08-31 | Albritton James R | Flared Energy Absorbing System and Method |
| CA2521520A1 (en) * | 2003-04-29 | 2004-11-11 | Amersham Biosciences Corp | Multiply-primed amplification of nucleic acid sequences |
| DE602004016440D1 (de) * | 2003-11-06 | 2008-10-23 | Rohm & Haas Elect Mat | Optischer Gegenstand mit leitender Struktur |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| EP1716254B1 (de) | 2004-02-19 | 2010-04-07 | Helicos Biosciences Corporation | Verfahren zur analyse von polynukleotidsequenzen |
| WO2005113147A2 (en) | 2004-04-08 | 2005-12-01 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| JP2008512084A (ja) | 2004-05-25 | 2008-04-24 | ヘリコス バイオサイエンシーズ コーポレイション | 核酸の配列決定のための方法およびデバイス |
| US7476734B2 (en) | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| ES2381279T3 (es) | 2005-05-06 | 2012-05-24 | Gen-Probe Incorporated | Métodos y productos para la captura de ácido nucleico diana |
| ITMI20051050A1 (it) * | 2005-06-07 | 2006-12-08 | Roberto Sapio | "metodo per la determinazione delle sequenze di acidi nucleici e analoghi" |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| US8568982B2 (en) * | 2006-08-09 | 2013-10-29 | National University Of Singapore | Methods of nucleic acid synthesis using particular crowding agents and concentrations |
| WO2010065629A2 (en) * | 2008-12-02 | 2010-06-10 | Kapabiosystems | Nucleic acid amplification |
| WO2010132508A2 (en) * | 2009-05-11 | 2010-11-18 | Biomatrica, Inc. | Compositions and methods for biological sample storage |
| WO2012018638A2 (en) | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
| EP2598661B1 (de) | 2010-07-26 | 2017-09-27 | Biomatrica, INC. | Zusammensetzungen zur stabilisierung von dna, rna und proteinen in speichel- und anderen biologischen proben während des transports und der lagerung bei umgebungstemperaturen |
| US9809798B2 (en) | 2011-03-11 | 2017-11-07 | National University Of Singapore | Pericyte progenitors from peripheral blood |
| EP2934572A4 (de) | 2012-12-20 | 2016-11-23 | Biomatrica Inc | Formulierungen und verfahren zur stabilisierung von pcr-reagenzien |
| WO2014100693A1 (en) * | 2012-12-21 | 2014-06-26 | Ibis Biosciences, Inc. | Stabilized nanopore and micropore structures and methods for making and using the same |
| JP6661554B2 (ja) | 2014-06-10 | 2020-03-11 | バイオマトリカ，インク． | 周囲温度における血小板の安定化 |
| CN106572974B (zh) | 2014-07-15 | 2021-04-23 | 生命技术公司 | 用于将分子有效递送到细胞的具有脂质聚集体的组合物和方法 |
| JP6827048B2 (ja) | 2015-12-08 | 2021-02-10 | バイオマトリカ，インク． | 赤血球沈降速度の低下 |
| KR102441122B1 (ko) * | 2020-05-11 | 2022-09-07 | 주식회사 엔지노믹스 | 폴리머라제 활성 측정 방법 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL100810A (en) * | 1992-01-30 | 1996-12-05 | Yeda Res & Dev | Pharmaceutical preparations including 2 - methyl - carboxy - 5 - hydroxy - tetrahydropyrimidine and / or 2 - methyl - 4 - carboxy - tetrahydropyrimidine, plywood methods |
| US5432065A (en) | 1993-03-30 | 1995-07-11 | United States Biochemical Corporation | Cycle sequencing with non-thermostable DNA polymerases |
| WO1995020682A1 (en) | 1994-01-31 | 1995-08-03 | The Regents Of The University Of California | Methods for the elimination of dna sequencing artifacts |
| US5545539A (en) | 1994-10-18 | 1996-08-13 | Genzyme Corporation | Method for nucleotide sequence amplification |
| FR2733515B1 (fr) | 1995-04-27 | 1997-09-05 | Cis Bio Int | Tampon, procede et methode d'evaluation des conditions optimales, d'amplification de sequences cibles d'acide nucleique, et leurs applications |
| GB9524974D0 (en) | 1995-12-06 | 1996-02-07 | Amersham Int Plc | An improved method for dye-terminator (cycle) sequencing |
| WO1998059075A1 (en) | 1997-06-25 | 1998-12-30 | Life Technologies, Inc. | Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence |
| IL123256A0 (en) * | 1998-02-10 | 1998-09-24 | Yeda Res & Dev | Methods for dna amplification and sequencing |
-
1998
- 1998-02-10 IL IL12325698A patent/IL123256A0/xx unknown
-
1999
- 1999-02-08 US US09/601,943 patent/US6428986B1/en not_active Expired - Lifetime
- 1999-02-08 ES ES99903891T patent/ES2323561T3/es not_active Expired - Lifetime
- 1999-02-08 WO PCT/IL1999/000080 patent/WO1999041410A1/en not_active Ceased
- 1999-02-08 AT AT99903891T patent/ATE427361T1/de not_active IP Right Cessation
- 1999-02-08 JP JP2000531590A patent/JP4393700B2/ja not_active Expired - Lifetime
- 1999-02-08 EP EP99903891A patent/EP1053350B1/de not_active Expired - Lifetime
- 1999-02-08 DE DE69940662T patent/DE69940662D1/de not_active Expired - Lifetime
-
2002
- 2002-04-08 US US10/117,077 patent/US7150980B1/en not_active Expired - Lifetime
-
2006
- 2006-08-31 US US11/513,206 patent/US7387878B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP4393700B2 (ja) | 2010-01-06 |
| EP1053350B1 (de) | 2009-04-01 |
| EP1053350A1 (de) | 2000-11-22 |
| IL123256A0 (en) | 1998-09-24 |
| US20070212760A1 (en) | 2007-09-13 |
| US7150980B1 (en) | 2006-12-19 |
| JP2002503482A (ja) | 2002-02-05 |
| WO1999041410A1 (en) | 1999-08-19 |
| US7387878B2 (en) | 2008-06-17 |
| US6428986B1 (en) | 2002-08-06 |
| ES2323561T3 (es) | 2009-07-20 |
| DE69940662D1 (de) | 2009-05-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |