ATE427361T1 - Verfahren zur dna-amplifikation und - sequenzierung - Google Patents
Verfahren zur dna-amplifikation und - sequenzierungInfo
- Publication number
- ATE427361T1 ATE427361T1 AT99903891T AT99903891T ATE427361T1 AT E427361 T1 ATE427361 T1 AT E427361T1 AT 99903891 T AT99903891 T AT 99903891T AT 99903891 T AT99903891 T AT 99903891T AT E427361 T1 ATE427361 T1 AT E427361T1
- Authority
- AT
- Austria
- Prior art keywords
- thp
- dna
- sequencing
- tetrahydropyrimidine
- proline
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 230000004544 DNA amplification Effects 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 abstract 2
- 238000002844 melting Methods 0.000 abstract 2
- 230000008018 melting Effects 0.000 abstract 2
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- WQXNXVUDBPYKBA-UHFFFAOYSA-N 2-Methyl-4-carboxy-3,4,5,6-tetrahydropyrimidine Chemical compound CC1=NCCC(C(O)=O)N1 WQXNXVUDBPYKBA-UHFFFAOYSA-N 0.000 abstract 1
- KIIBBJKLKFTNQO-UHFFFAOYSA-N 5-hydroxy-2-methyl-1,4,5,6-tetrahydropyrimidin-3-ium-6-carboxylate Chemical compound CC1=NCC(O)C(C(O)=O)N1 KIIBBJKLKFTNQO-UHFFFAOYSA-N 0.000 abstract 1
- 238000001712 DNA sequencing Methods 0.000 abstract 1
- 230000006820 DNA synthesis Effects 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL12325698A IL123256A0 (en) | 1998-02-10 | 1998-02-10 | Methods for dna amplification and sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE427361T1 true ATE427361T1 (de) | 2009-04-15 |
Family
ID=11071218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99903891T ATE427361T1 (de) | 1998-02-10 | 1999-02-08 | Verfahren zur dna-amplifikation und - sequenzierung |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US6428986B1 (de) |
| EP (1) | EP1053350B1 (de) |
| JP (1) | JP4393700B2 (de) |
| AT (1) | ATE427361T1 (de) |
| DE (1) | DE69940662D1 (de) |
| ES (1) | ES2323561T3 (de) |
| IL (1) | IL123256A0 (de) |
| WO (1) | WO1999041410A1 (de) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL123256A0 (en) * | 1998-02-10 | 1998-09-24 | Yeda Res & Dev | Methods for dna amplification and sequencing |
| US6787305B1 (en) | 1998-03-13 | 2004-09-07 | Invitrogen Corporation | Compositions and methods for enhanced synthesis of nucleic acid molecules |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| NZ512244A (en) | 1998-11-12 | 2003-12-19 | Invitrogen Corp | Polycationic transfection reagents for introducing anions into a cell |
| US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US7306397B2 (en) * | 2002-07-22 | 2007-12-11 | Exodyne Technologies, Inc. | Energy attenuating safety system |
| US7101111B2 (en) * | 1999-07-19 | 2006-09-05 | Exodyne Technologies Inc. | Flared energy absorbing system and method |
| AU7733200A (en) * | 1999-09-30 | 2001-04-30 | Qiagen Genomics, Inc. | Compositions and methods for reducing oligonucleotide hybridization and priming specificity |
| CN1326990A (zh) * | 2000-06-07 | 2001-12-19 | 上海博德基因开发有限公司 | 一种新的多肽——人类dna cgg重复结合蛋白16.17和编码这种多肽的多核苷酸 |
| JP2004523243A (ja) | 2001-03-12 | 2004-08-05 | カリフォルニア インスティチュート オブ テクノロジー | 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置 |
| AU2003272174A1 (en) * | 2002-10-21 | 2004-05-04 | Biotage Ab | Use of osmolytes to heat stabilise luciferase and/or apyrase |
| EP2031070B1 (de) | 2002-12-04 | 2013-07-17 | Life Technologies Corporation | Multiplexverstärkung von Polynucleotiden |
| US20060193688A1 (en) * | 2003-03-05 | 2006-08-31 | Albritton James R | Flared Energy Absorbing System and Method |
| EP1618187A4 (de) * | 2003-04-29 | 2006-12-20 | Ge Healthcare Bio Sciences | Amplifikation von nukleinsäuresequenzen mit mehrfachpriming |
| DE602004016440D1 (de) * | 2003-11-06 | 2008-10-23 | Rohm & Haas Elect Mat | Optischer Gegenstand mit leitender Struktur |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| WO2005080605A2 (en) | 2004-02-19 | 2005-09-01 | Helicos Biosciences Corporation | Methods and kits for analyzing polynucleotide sequences |
| WO2005113147A2 (en) | 2004-04-08 | 2005-12-01 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| US7635562B2 (en) | 2004-05-25 | 2009-12-22 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
| US7476734B2 (en) | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| ATE551433T1 (de) | 2005-05-06 | 2012-04-15 | Gen Probe Inc | Verfahren und produkte zum erfassen von nukleinsäurezielen |
| ITMI20051050A1 (it) * | 2005-06-07 | 2006-12-08 | Roberto Sapio | "metodo per la determinazione delle sequenze di acidi nucleici e analoghi" |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| EP2057289B1 (de) * | 2006-08-09 | 2016-08-10 | Univ Singapore | Verfahren für molekularbiologische anwendungen |
| WO2010065629A2 (en) * | 2008-12-02 | 2010-06-10 | Kapabiosystems | Nucleic acid amplification |
| EP2430195B1 (de) * | 2009-05-11 | 2019-01-23 | Biomatrica, INC. | Zusammensetzungen und verfahren zur lagerung biologischer proben |
| EP2598660B1 (de) | 2010-07-26 | 2017-03-15 | Biomatrica, INC. | Zusammensetzungen zur stabilisierung von dna, rna und proteinen in blut- und anderen biologischen proben während des transports und der lagerung bei umgebungstemperaturen |
| WO2012018639A2 (en) | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures |
| SG10201601853XA (en) | 2011-03-11 | 2016-04-28 | Univ Singapore | Pericyte progenitors from peripheral blood |
| US9725703B2 (en) | 2012-12-20 | 2017-08-08 | Biomatrica, Inc. | Formulations and methods for stabilizing PCR reagents |
| US20150346188A1 (en) * | 2012-12-21 | 2015-12-03 | Ibis Biosciences, Inc. | Stabilized nanopore and micropore structures and methods for making and using the same |
| ES2786373T3 (es) | 2014-06-10 | 2020-10-09 | Biomatrica Inc | Estabilización de trombocitos a temperaturas ambiente |
| EP3169310A1 (de) | 2014-07-15 | 2017-05-24 | Life Technologies Corporation | Zusammensetzungen mit lipidaggregaten und verfahren zur effizienten bereitstellung von molekülen an zellen |
| KR20250047404A (ko) | 2015-12-08 | 2025-04-03 | 바이오매트리카 인코포레이티드 | 적혈구 침강 속도의 감소 |
| KR102441122B1 (ko) * | 2020-05-11 | 2022-09-07 | 주식회사 엔지노믹스 | 폴리머라제 활성 측정 방법 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL100810A (en) | 1992-01-30 | 1996-12-05 | Yeda Res & Dev | Pharmaceutical preparations including 2 - methyl - carboxy - 5 - hydroxy - tetrahydropyrimidine and / or 2 - methyl - 4 - carboxy - tetrahydropyrimidine, plywood methods |
| US5432065A (en) * | 1993-03-30 | 1995-07-11 | United States Biochemical Corporation | Cycle sequencing with non-thermostable DNA polymerases |
| DE69531487T2 (de) | 1994-01-31 | 2004-06-17 | The Regents Of The University Of California, Oakland | Methode zur eliminierung von sequenzierartefakten |
| US5545539A (en) * | 1994-10-18 | 1996-08-13 | Genzyme Corporation | Method for nucleotide sequence amplification |
| FR2733515B1 (fr) * | 1995-04-27 | 1997-09-05 | Cis Bio Int | Tampon, procede et methode d'evaluation des conditions optimales, d'amplification de sequences cibles d'acide nucleique, et leurs applications |
| GB9524974D0 (en) * | 1995-12-06 | 1996-02-07 | Amersham Int Plc | An improved method for dye-terminator (cycle) sequencing |
| JP2002506352A (ja) | 1997-06-25 | 2002-02-26 | ライフ テクノロジーズ，インコーポレイテッド | 所望のヌクレオチド配列を有する標的ｄｎａまたはｒｎａ分子を単離および回収するための改良された方法 |
| IL123256A0 (en) * | 1998-02-10 | 1998-09-24 | Yeda Res & Dev | Methods for dna amplification and sequencing |
-
1998
- 1998-02-10 IL IL12325698A patent/IL123256A0/xx unknown
-
1999
- 1999-02-08 DE DE69940662T patent/DE69940662D1/de not_active Expired - Lifetime
- 1999-02-08 JP JP2000531590A patent/JP4393700B2/ja not_active Expired - Lifetime
- 1999-02-08 US US09/601,943 patent/US6428986B1/en not_active Expired - Lifetime
- 1999-02-08 WO PCT/IL1999/000080 patent/WO1999041410A1/en not_active Ceased
- 1999-02-08 EP EP99903891A patent/EP1053350B1/de not_active Expired - Lifetime
- 1999-02-08 AT AT99903891T patent/ATE427361T1/de not_active IP Right Cessation
- 1999-02-08 ES ES99903891T patent/ES2323561T3/es not_active Expired - Lifetime
-
2002
- 2002-04-08 US US10/117,077 patent/US7150980B1/en not_active Expired - Lifetime
-
2006
- 2006-08-31 US US11/513,206 patent/US7387878B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999041410A1 (en) | 1999-08-19 |
| US20070212760A1 (en) | 2007-09-13 |
| ES2323561T3 (es) | 2009-07-20 |
| US7387878B2 (en) | 2008-06-17 |
| DE69940662D1 (de) | 2009-05-14 |
| JP4393700B2 (ja) | 2010-01-06 |
| US6428986B1 (en) | 2002-08-06 |
| IL123256A0 (en) | 1998-09-24 |
| EP1053350B1 (de) | 2009-04-01 |
| US7150980B1 (en) | 2006-12-19 |
| EP1053350A1 (de) | 2000-11-22 |
| JP2002503482A (ja) | 2002-02-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |