ATE427361T1 - Verfahren zur dna-amplifikation und - sequenzierung - Google Patents

Verfahren zur dna-amplifikation und - sequenzierung

Info

Publication number: ATE427361T1
Authority: AT; Austria
Prior art keywords: thp; dna; sequencing; tetrahydropyrimidine; proline
Prior art date: 1998-02-10

Application number

AT99903891T

Other languages

English (en)

Inventor

Aviva Lapidot

Robert Iakobashvili

Gennady Malin

Original Assignee

Yeda Res & Dev

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-02-10

Filing date

1999-02-08

Publication date

2009-04-15

1999-02-08 Application filed by Yeda Res & Dev filed Critical Yeda Res & Dev

2009-04-15 Application granted granted Critical

2009-04-15 Publication of ATE427361T1 publication Critical patent/ATE427361T1/de

Links

238000000034 method Methods 0.000 title abstract 2
230000004544 DNA amplification Effects 0.000 title 1
238000012163 sequencing technique Methods 0.000 title 1
108020004414 DNA Proteins 0.000 abstract 2
102000053602 DNA Human genes 0.000 abstract 2
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 abstract 2
238000002844 melting Methods 0.000 abstract 2
230000008018 melting Effects 0.000 abstract 2
238000003752 polymerase chain reaction Methods 0.000 abstract 2
WQXNXVUDBPYKBA-UHFFFAOYSA-N 2-Methyl-4-carboxy-3,4,5,6-tetrahydropyrimidine Chemical compound CC1=NCCC(C(O)=O)N1 WQXNXVUDBPYKBA-UHFFFAOYSA-N 0.000 abstract 1
KIIBBJKLKFTNQO-UHFFFAOYSA-N 5-hydroxy-2-methyl-1,4,5,6-tetrahydropyrimidin-3-ium-6-carboxylate Chemical compound CC1=NCC(O)C(C(O)=O)N1 KIIBBJKLKFTNQO-UHFFFAOYSA-N 0.000 abstract 1
238000001712 DNA sequencing Methods 0.000 abstract 1
230000006820 DNA synthesis Effects 0.000 abstract 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 abstract 1
230000003321 amplification Effects 0.000 abstract 1
230000001404 mediated effect Effects 0.000 abstract 1
238000003199 nucleic acid amplification method Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Physics & Mathematics (AREA)
Biotechnology (AREA)
Analytical Chemistry (AREA)
Biophysics (AREA)
Bioinformatics & Cheminformatics (AREA)
Immunology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Chemical Kinetics & Catalysis (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Enzymes And Modification Thereof (AREA)
Nitrogen Condensed Heterocyclic Rings (AREA)

AT99903891T 1998-02-10 1999-02-08 Verfahren zur dna-amplifikation und - sequenzierung ATE427361T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
IL12325698A IL123256A0 (en)	1998-02-10	1998-02-10	Methods for dna amplification and sequencing

Publications (1)

Publication Number	Publication Date
ATE427361T1 true ATE427361T1 (de)	2009-04-15

Family

ID=11071218

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT99903891T ATE427361T1 (de)	1998-02-10	1999-02-08	Verfahren zur dna-amplifikation und - sequenzierung

Country Status (8)

Country	Link
US (3)	US6428986B1 (de)
EP (1)	EP1053350B1 (de)
JP (1)	JP4393700B2 (de)
AT (1)	ATE427361T1 (de)
DE (1)	DE69940662D1 (de)
ES (1)	ES2323561T3 (de)
IL (1)	IL123256A0 (de)
WO (1)	WO1999041410A1 (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL123256A0 (en) *	1998-02-10	1998-09-24	Yeda Res & Dev	Methods for dna amplification and sequencing
US6787305B1 (en)	1998-03-13	2004-09-07	Invitrogen Corporation	Compositions and methods for enhanced synthesis of nucleic acid molecules
US7875440B2 (en)	1998-05-01	2011-01-25	Arizona Board Of Regents	Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en)	1998-05-01	2004-08-24	Arizona Board Of Regents	Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
EP1829856A3 (de)	1998-11-12	2009-02-25	Invitrogen Corporation	Transfektionsreagenzien
US7501245B2 (en)	1999-06-28	2009-03-10	Helicos Biosciences Corp.	Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en)	1999-06-28	2004-11-16	California Institute Of Technology	Methods and apparatus for analyzing polynucleotide sequences
US7306397B2 (en) *	2002-07-22	2007-12-11	Exodyne Technologies, Inc.	Energy attenuating safety system
US7101111B2 (en) *	1999-07-19	2006-09-05	Exodyne Technologies Inc.	Flared energy absorbing system and method
WO2001023618A2 (en) *	1999-09-30	2001-04-05	Qiagen Genomics, Inc.	Compositions and methods for reducing oligonucleotide hybridization and priming specificity
CN1326990A (zh) *	2000-06-07	2001-12-19	上海博德基因开发有限公司	一种新的多肽——人类dna cgg重复结合蛋白16.17和编码这种多肽的多核苷酸
WO2002072892A1 (en)	2001-03-12	2002-09-19	California Institute Of Technology	Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
AU2003272174A1 (en) *	2002-10-21	2004-05-04	Biotage Ab	Use of osmolytes to heat stabilise luciferase and/or apyrase
EP3000899A1 (de)	2002-12-04	2016-03-30	Applied Biosystems, LLC	Multiplexverstärkung von polynucleotiden
US20060193688A1 (en) *	2003-03-05	2006-08-31	Albritton James R	Flared Energy Absorbing System and Method
JP2006525023A (ja) *	2003-04-29	2006-11-09	ジーイー・ヘルスケア・バイオサイエンス・コーポレイション	核酸配列の多重開始増幅
EP1530065B1 (de) *	2003-11-06	2008-09-10	Rohm and Haas Electronic Materials, L.L.C.	Optischer Gegenstand mit leitender Struktur
US7169560B2 (en)	2003-11-12	2007-01-30	Helicos Biosciences Corporation	Short cycle methods for sequencing polynucleotides
EP2248911A1 (de)	2004-02-19	2010-11-10	Helicos Biosciences Corporation	Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
AU2005245338B2 (en)	2004-04-08	2011-11-17	Biomatrica, Inc.	Integration of sample storage and sample management for life science
CA2566806A1 (en)	2004-05-25	2006-01-19	Helicos Biosciences Corporation	Methods and devices for nucleic acid sequence determination
US7476734B2 (en)	2005-12-06	2009-01-13	Helicos Biosciences Corporation	Nucleotide analogs
US7220549B2 (en)	2004-12-30	2007-05-22	Helicos Biosciences Corporation	Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en)	2005-01-28	2009-01-27	Helicos Biosciences Corporation	Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
CA2584230C (en)	2005-05-06	2012-11-27	Gen-Probe Incorporated	Methods of nucleic acid target capture
ITMI20051050A1 (it) *	2005-06-07	2006-12-08	Roberto Sapio	"metodo per la determinazione delle sequenze di acidi nucleici e analoghi"
US7666593B2 (en)	2005-08-26	2010-02-23	Helicos Biosciences Corporation	Single molecule sequencing of captured nucleic acids
US7397546B2 (en)	2006-03-08	2008-07-08	Helicos Biosciences Corporation	Systems and methods for reducing detected intensity non-uniformity in a laser beam
WO2008018839A1 (en) *	2006-08-09	2008-02-14	National University Of Singapore	Method for molecular biology applications
US20110294167A1 (en) *	2008-12-02	2011-12-01	Kapabiosystems	Nucleic acid amplification
EP2430195B1 (de) *	2009-05-11	2019-01-23	Biomatrica, INC.	Zusammensetzungen und verfahren zur lagerung biologischer proben
WO2012018639A2 (en)	2010-07-26	2012-02-09	Biomatrica, Inc.	Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012018638A2 (en)	2010-07-26	2012-02-09	Biomatrica, Inc.	Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
SG192862A1 (en)	2011-03-11	2013-09-30	Univ Singapore	Pericyte progenitors from peripheral blood
EP3249054A1 (de)	2012-12-20	2017-11-29	Biomatrica, INC.	Formulierungen und verfahren zur stabilisierung von pcr-reagenzien
US20150346188A1 (en) *	2012-12-21	2015-12-03	Ibis Biosciences, Inc.	Stabilized nanopore and micropore structures and methods for making and using the same
WO2015191632A1 (en)	2014-06-10	2015-12-17	Biomatrica, Inc.	Stabilization of thrombocytes at ambient temperatures
WO2016011203A1 (en)	2014-07-15	2016-01-21	Life Technologies Corporation	Compositions with lipid aggregates and methods for efficient delivery of molecules to cells
KR102589056B1 (ko)	2015-12-08	2023-10-12	바이오매트리카 인코포레이티드	적혈구 침강 속도의 감소
KR102441122B1 (ko) *	2020-05-11	2022-09-07	주식회사 엔지노믹스	폴리머라제 활성 측정 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL100810A (en) *	1992-01-30	1996-12-05	Yeda Res & Dev	Pharmaceutical preparations including 2 - methyl - carboxy - 5 - hydroxy - tetrahydropyrimidine and / or 2 - methyl - 4 - carboxy - tetrahydropyrimidine, plywood methods
US5432065A (en)	1993-03-30	1995-07-11	United States Biochemical Corporation	Cycle sequencing with non-thermostable DNA polymerases
ATE247175T1 (de)	1994-01-31	2003-08-15	Univ California	Methode zur eliminierung von sequenzierartefakten
US5545539A (en)	1994-10-18	1996-08-13	Genzyme Corporation	Method for nucleotide sequence amplification
FR2733515B1 (fr)	1995-04-27	1997-09-05	Cis Bio Int	Tampon, procede et methode d'evaluation des conditions optimales, d'amplification de sequences cibles d'acide nucleique, et leurs applications
GB9524974D0 (en)	1995-12-06	1996-02-07	Amersham Int Plc	An improved method for dye-terminator (cycle) sequencing
AU8162598A (en)	1997-06-25	1999-01-04	Life Technologies, Inc.	Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
IL123256A0 (en) *	1998-02-10	1998-09-24	Yeda Res & Dev	Methods for dna amplification and sequencing

1998
- 1998-02-10 IL IL12325698A patent/IL123256A0/xx unknown
1999
- 1999-02-08 AT AT99903891T patent/ATE427361T1/de not_active IP Right Cessation
- 1999-02-08 WO PCT/IL1999/000080 patent/WO1999041410A1/en not_active Ceased
- 1999-02-08 JP JP2000531590A patent/JP4393700B2/ja not_active Expired - Lifetime
- 1999-02-08 DE DE69940662T patent/DE69940662D1/de not_active Expired - Lifetime
- 1999-02-08 US US09/601,943 patent/US6428986B1/en not_active Expired - Lifetime
- 1999-02-08 EP EP99903891A patent/EP1053350B1/de not_active Expired - Lifetime
- 1999-02-08 ES ES99903891T patent/ES2323561T3/es not_active Expired - Lifetime
2002
- 2002-04-08 US US10/117,077 patent/US7150980B1/en not_active Expired - Lifetime
2006
- 2006-08-31 US US11/513,206 patent/US7387878B2/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
DE69940662D1 (de)	2009-05-14
US7150980B1 (en)	2006-12-19
WO1999041410A1 (en)	1999-08-19
US20070212760A1 (en)	2007-09-13
EP1053350B1 (de)	2009-04-01
JP4393700B2 (ja)	2010-01-06
JP2002503482A (ja)	2002-02-05
EP1053350A1 (de)	2000-11-22
IL123256A0 (en)	1998-09-24
US6428986B1 (en)	2002-08-06
ES2323561T3 (es)	2009-07-20
US7387878B2 (en)	2008-06-17

Legal Events

Date	Code	Title	Description
2009-10-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
DE69940662D1 (de)	2009-05-14	Verfahren zur dna-amplifikation und -sequenzierung
EP2272952B8 (de)	2012-08-15	Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
DK0669401T3 (da)	2002-04-02	Amplificering af lange nukleinsyresekvenser ved PCR
DE69835008D1 (de)	2006-08-03	Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)
DK1040202T3 (da)	2009-04-14	DNA-polymorfiidentitetsbestemmelse under anvendelse af flowcytometri
DE69637805D1 (de)	2009-02-26	Nukleinsäure liganden die dna-polymerase binden und inhibieren
DE60233462D1 (de)	2009-10-08	Neue Zusammensetzung und Methode zur 'hot start' Nukleinsäureamplifizierung
ATE194015T1 (de)	2000-07-15	Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung
ATE360093T1 (de)	2007-05-15	Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure
DK1171632T3 (da)	2010-11-08	Nucleinsyreligand-inhibitorer af DNA-polymeraser
ES2186293T3 (es)	2003-05-01	Metodo para la amplificacion exponencial directa y secuenciacion de moleculas de dna y su aplicacion.
WO2004022770A3 (en)	2004-08-05	Compositions and methods for synthesizing nucleic acids
WO2001025483A3 (en)	2002-01-24	Compositions for dna amplification, synthesis, and mutagenesis
ATE330031T1 (de)	2006-07-15	Reversibel inhibierende sonden
ATE484515T1 (de)	2010-10-15	Dna-polymerase-mischungen und mutante dna- polymerasen
NO991566D0 (no)	1999-03-30	Termostabil nukleinsyre og polymerase fra Thermococcus gorgonarius
CA3140836A1 (en)	2014-05-30	A method of removing amplicons of a non-target nucleic acid having one or more methylated cytosines from a sample
ATE526418T1 (de)	2011-10-15	Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
DE60024464D1 (de)	2006-01-05	Hohe dichte markierung von dns mit modifizierte oder chromophore tragende nukleotide und benutzte dns polymerasen
WO2004067775A3 (en)	2004-12-09	Method for the detection of cytosine methylation patterns with high sensitivity