ATE491810T1 - Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung - Google Patents

Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung

Info

Publication number
ATE491810T1
ATE491810T1 AT08075840T AT08075840T ATE491810T1 AT E491810 T1 ATE491810 T1 AT E491810T1 AT 08075840 T AT08075840 T AT 08075840T AT 08075840 T AT08075840 T AT 08075840T AT E491810 T1 ATE491810 T1 AT E491810T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid amplification
novel nucleotide
nucleotide mixture
amplification performance
Prior art date
Application number
AT08075840T
Other languages
English (en)
Inventor
Brian Washburn
Douglas Lovern
Original Assignee
Bio Merieux Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux Inc filed Critical Bio Merieux Inc
Application granted granted Critical
Publication of ATE491810T1 publication Critical patent/ATE491810T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
AT08075840T 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung ATE491810T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US53881604P 2004-01-23 2004-01-23
US53881504P 2004-01-23 2004-01-23
US53881404P 2004-01-23 2004-01-23

Publications (1)

Publication Number Publication Date
ATE491810T1 true ATE491810T1 (de) 2011-01-15

Family

ID=34812082

Family Applications (3)

Application Number Title Priority Date Filing Date
AT08075840T ATE491810T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung
AT05712046T ATE481507T1 (de) 2004-01-23 2005-01-21 Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv
AT05722543T ATE417938T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren

Family Applications After (2)

Application Number Title Priority Date Filing Date
AT05712046T ATE481507T1 (de) 2004-01-23 2005-01-21 Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv
AT05722543T ATE417938T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren

Country Status (6)

Country Link
US (3) US7364854B2 (de)
EP (3) EP1706512B1 (de)
JP (2) JP2007518425A (de)
AT (3) ATE491810T1 (de)
DE (3) DE602005023586D1 (de)
WO (2) WO2005070027A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008134374A1 (en) * 2007-04-27 2008-11-06 Eragen Biosciences, Inc. Materials and methods for detection of hepatitis c virus
JP2009284896A (ja) 2007-07-26 2009-12-10 Fujifilm Corp 核酸増幅方法
US20090162856A1 (en) * 2007-11-06 2009-06-25 Hayato Miyoshi Rna detection method
AU2009335156A1 (en) * 2008-12-31 2010-07-08 Abbott Laboratories Primers and probes for detecting Hepatitis C virus

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09107970A (ja) * 1995-10-17 1997-04-28 Soyaku Gijutsu Kenkyusho:Kk Ｃ型肝炎ウイルス由来核酸、該核酸を用いたウイルスの検出方法
EP0052296B1 (de) * 1980-11-12 1984-06-27 Mitsubishi Kasei Corporation Als Heilmittel für Immunokrankheiten anwendbare Glutaminderivate, Verfahren zu deren Herstellung und Zusammensetzungen, die die obengenannten Derivate enthalten
US5087565A (en) * 1984-07-02 1992-02-11 Du Pont Merck Pharmaceutical Company Synthetic process for preparation of 32 P-labeled nucleotides
DE3689223T2 (de) 1985-05-15 1994-03-03 Amoco Corp Cytidinanaloga.
US4876187A (en) 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5032511A (en) * 1987-03-31 1991-07-16 Mitsubishi Kasei Corporation DNA fragments coding for antigens specific to non-A non-B hepatitis, expression vectors containing said DNA fragments, transformants and process for producing said antigens
IE72468B1 (en) 1987-07-31 1997-04-09 Univ Leland Stanford Junior Selective amplification of target polynucleotide sequences
US5712088A (en) * 1987-11-18 1998-01-27 Chiron Corporation Methods for detecting Hepatitis C virus using polynucleotides specific for same
US5698390A (en) * 1987-11-18 1997-12-16 Chiron Corporation Hepatitis C immunoassays
US5714596A (en) * 1987-11-18 1998-02-03 Chiron Corporation NANBV diagnostics: polynucleotides useful for screening for hepatitis C virus
US5030557A (en) 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
EP0578329B1 (de) 1989-09-18 1996-03-13 Canon Kabushiki Kaisha Tintenstrahlaufzeichnungskopf und Tintenstrahlgerät mit diesem Kopf
CA2075050C (en) * 1990-02-16 1998-10-06 Will Bloch Specificity and convenience of the polymerase chain reaction
WO1993022461A1 (en) 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US5648232A (en) * 1993-01-21 1997-07-15 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Microbiological best method and reagents
JP3062250B2 (ja) * 1994-03-10 2000-07-10 ジェン−プローブ・インコーポレイテッド イオン性界面活性剤による、酵素により媒介される反応に対する阻害の抑制方法
US6127116A (en) * 1995-08-29 2000-10-03 Washington University Functional DNA clone for hepatitis C virus (HCV) and uses thereof
US5874565A (en) * 1995-08-29 1999-02-23 Washington University Nucleic acids comprising a highly conserved novel 3 terminal sequence element of the hepatitis C virus
DE19612779A1 (de) * 1996-03-29 1997-10-02 Boehringer Mannheim Gmbh Verfahren zur spezifischen Vervielfältigung von langen Nukleinsäuren durch PCR
WO1998006736A1 (en) * 1996-08-14 1998-02-19 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
DE19647055A1 (de) * 1996-11-14 1998-05-20 Boehringer Mannheim Gmbh Stabilisierte wässrige Nukleosidtriphosphat-Lösungen
EP1705253B1 (de) * 1997-04-03 2010-11-03 Life Technologies Corporation Verfahren und Produkte zur reversen Transkription gekoppelt mit Polymerasekettenreaktion (RT-PCR)
ZA989950B (en) 1997-11-17 1999-05-04 Akzo Nobel Nv Transcription based amplification of double stranded DNA targets
KR20010043111A (ko) * 1998-04-27 2001-05-25 랄프 이. 크리스토퍼슨 Ｃ형 간염 바이러스성 감염과 관련된 질환의 효소적 핵산치료제
WO2000000638A2 (en) 1998-06-26 2000-01-06 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
US6300075B1 (en) 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
US6638714B1 (en) 1999-02-03 2003-10-28 Ortho-Clinical Diagnostics, Inc. Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof
WO2000075338A2 (en) * 1999-06-04 2000-12-14 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services CLONED GENONE OF INFECTIOUS HEPATITIS C VIRUS OF GENOTYPE 2a AND USES THEREOF
US6271004B1 (en) * 1999-06-25 2001-08-07 Display Systems Biotech A/S Method for improved reverse transcription at high temperatures
CA2392218A1 (en) * 1999-11-17 2001-05-25 Jiuping Ji Simultaneous detection of hbv, hcv and hiv in plasma samples using a multiplex capture assay
WO2001064959A1 (en) 2000-03-02 2001-09-07 Akzo Nobel N.V. Detection of hepatitis b virus rna
CA2442092A1 (en) * 2001-03-26 2002-10-17 Ribozyme Pharmaceuticals, Inc. Oligonucleotide mediated inhibition of hepatitis b virus and hepatitis c virus replication
WO2003016572A1 (en) * 2001-08-17 2003-02-27 Eli Lilly And Company Oligonucleotide therapeutics for treating hepatitis c virus infections
US6783940B2 (en) * 2001-10-31 2004-08-31 Applera Corporation Method of reducing non-specific amplification in PCR
US6750009B2 (en) 2002-01-29 2004-06-15 Apath, Llc Multiple viral replicon culture systems
WO2003100014A2 (en) 2002-05-24 2003-12-04 Smithkline Beecham Corporation Method for quantitating negative strand rna synthesis

Also Published As

Publication number Publication date
EP1721015B1 (de) 2010-09-15
ATE481507T1 (de) 2010-10-15
US20060003343A1 (en) 2006-01-05
EP1706512A2 (de) 2006-10-04
JP2007518425A (ja) 2007-07-12
ATE417938T1 (de) 2009-01-15
EP2042612A3 (de) 2009-06-17
WO2005071117A2 (en) 2005-08-04
US7670779B2 (en) 2010-03-02
EP1721015A2 (de) 2006-11-15
JP4866248B2 (ja) 2012-02-01
DE602005023586D1 (de) 2010-10-28
EP2042612A2 (de) 2009-04-01
WO2005070027A2 (en) 2005-08-04
WO2005070027A3 (en) 2006-04-06
DE602005025424D1 (de) 2011-01-27
US7364854B2 (en) 2008-04-29
US20060019266A1 (en) 2006-01-26
US20080248480A1 (en) 2008-10-09
JP2007518424A (ja) 2007-07-12
WO2005071117A3 (en) 2006-04-20
EP1706512B1 (de) 2008-12-17
WO2005070027A8 (en) 2006-07-27
DE602005011754D1 (de) 2009-01-29
EP2042612B1 (de) 2010-12-15

Similar Documents

Publication Publication Date Title
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
ATE447040T1 (de) Verbesserte zusammensetzungen und verfahren für die cdna-synthese
BR0101493B1 (pt) mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes.
ATE550441T1 (de) Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
ATE502122T1 (de) Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
MY143596A (en) In vitro recombination method
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
WO2008051530A3 (en) Polymerase enzymes and reagents for enhanced nucleic acid sequencing
DE60230672D1 (de) Nukleinsäureanreicherung
DK1929012T3 (da) Fremgangsmåde til in vitro-rekombination
ATE458832T1 (de) Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
DK2357226T3 (da) Rationelt udformede meganukleaser med ændret sekvensspecificitet og DNA-bindingsaffinitet
DK1507002T3 (da) Termostabilt Taq-polymerasefragment
IL225849A0 (en) Molecules with binding ability that can neutralize rabies virus, and their uses
DE602005023588D1 (de) Verfahren zur genotypisierung von antigenen von blutzellen und ein kit zur genotypisierung
SE0301951D0 (sv) New method
BRPI0516619A (pt) reagentes e métodos para reprodutibilidade aperfeiçoada e redução de iniciação errÈnia em amplificação por pcr
ATE356871T1 (de) Eine methode zur in vitro molekularen evolution der proteinfunktion
WO2003004703A1 (fr) Processus de production de matrice d'adn et processus de production de proteine dans un systeme de synthese de proteine sans cellule a l'aide du premier processus
EP1904518A4 (de) Dna-polymerase-mischungen und mutante dna-polymerasen
ATE497022T1 (de) Verfahren zur amplifikation von nukleinsäure
DE602005025424D1 (de) Neuartiges Nukleotidgemisch für eine verbesserte Nukleinsäurenamplifikationsleistung
DE602005017252D1 (de) Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates
DE60331072D1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
RU2006134100A (ru) Способ проведения аллель-специфичной пцр для генотипирования крупного рогатого скота по аллелям а и в гена каппа-казеина

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties