ATE444359T1 - Verfahren zur erzeugung einer hohen antikörperproduktion von durch in-vitro- immunisierung entstandenen hybridomen - Google Patents

Verfahren zur erzeugung einer hohen antikörperproduktion von durch in-vitro- immunisierung entstandenen hybridomen

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Publication number
ATE444359T1
ATE444359T1 AT03786774T AT03786774T ATE444359T1 AT E444359 T1 ATE444359 T1 AT E444359T1 AT 03786774 T AT03786774 T AT 03786774T AT 03786774 T AT03786774 T AT 03786774T AT E444359 T1 ATE444359 T1 AT E444359T1
Authority
AT
Austria
Prior art keywords
cells
mismatch repair
affinity antibodies
generating high
hybridoma
Prior art date
Application number
AT03786774T
Other languages
English (en)
Inventor
Luigi Grasso
Shaohong Liang
Nicholas Nicolaides
Philip Sass
Original Assignee
Morphotek Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morphotek Inc filed Critical Morphotek Inc
Application granted granted Critical
Publication of ATE444359T1 publication Critical patent/ATE444359T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1282—Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/243—Colony Stimulating Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT03786774T 2002-11-15 2003-11-14 Verfahren zur erzeugung einer hohen antikörperproduktion von durch in-vitro- immunisierung entstandenen hybridomen ATE444359T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42716502P 2002-11-15 2002-11-15
US50165003P 2003-09-10 2003-09-10
PCT/US2003/036702 WO2004046330A2 (en) 2002-11-15 2003-11-14 Methods of generating high-production of antibodies from hybridomas created by in vitro immunization

Publications (1)

Publication Number Publication Date
ATE444359T1 true ATE444359T1 (de) 2009-10-15

Family

ID=32329149

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03786774T ATE444359T1 (de) 2002-11-15 2003-11-14 Verfahren zur erzeugung einer hohen antikörperproduktion von durch in-vitro- immunisierung entstandenen hybridomen

Country Status (8)

Country Link
US (2) US7754450B2 (de)
EP (1) EP1572971B1 (de)
JP (1) JP4555089B2 (de)
AT (1) ATE444359T1 (de)
AU (1) AU2003295576B2 (de)
CA (1) CA2506127C (de)
DE (1) DE60329526D1 (de)
WO (1) WO2004046330A2 (de)

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US6982169B2 (en) * 2001-01-15 2006-01-03 Morphotek, Inc. Chemical inhibitors of mismatch repair
JP4555089B2 (ja) 2002-11-15 2010-09-29 モーフオテク・インコーポレーテツド ｉｎｖｉｔｒｏ免疫感作により創製されたハイブリドーマからの抗体の高生産量の生成方法
RU2447085C2 (ru) 2005-05-18 2012-04-10 МорфоСис АГ Анти-gm-csf антитела и их применение
US7741450B2 (en) 2006-02-08 2010-06-22 Morphotek Inc. Antibodies to GM-CSF
AU2013201228B2 (en) * 2006-02-08 2016-01-21 Eisai, Inc. Antigenic gm-csf peptides and antibodies to gm-csf
JP2009533072A (ja) * 2006-04-17 2009-09-17 モルフォテック、インク． 細胞によるタンパク質および抗体収量を改善するために適用される全ゲノム進化技術
US20090075378A1 (en) 2007-02-20 2009-03-19 Anaptysbio, Inc. Somatic hypermutation systems
US20100248971A1 (en) * 2007-12-03 2010-09-30 Takashi Inagaki Process for antibody production
EP2289937A1 (de) * 2009-08-24 2011-03-02 Wilfried Stücker Herstellung von monoklonalen In-vitro-Antikörpern
WO2011159959A2 (en) * 2010-06-16 2011-12-22 Cdi Laboratories Methods and systems for generating, validating and using monoclonal antibodies
EP2890787B1 (de) 2012-08-31 2019-05-01 Synthetic Genomics, Inc. Crowding-agent-induzierter nukleinsäuretransfer in eine empfängerwirtszelle
US10260089B2 (en) 2012-10-29 2019-04-16 The Research Foundation Of The State University Of New York Compositions and methods for recognition of RNA using triple helical peptide nucleic acids
US20140273031A1 (en) * 2013-03-15 2014-09-18 The Cleveland Clinic Foundation In-vitro method for monoclonal antibody production
CA2906737C (en) * 2013-03-15 2023-08-15 Amgen Inc. Human pac1 antibodies
CN110776564B (zh) * 2019-10-30 2022-02-08 西北农林科技大学 两株抗新城疫病毒纳米抗体及其表达制备方法和应用

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CA2506127C (en) 2013-07-09
AU2003295576B2 (en) 2011-03-17
US8445229B2 (en) 2013-05-21
EP1572971A4 (de) 2007-07-04
JP4555089B2 (ja) 2010-09-29
US20040214288A1 (en) 2004-10-28
CA2506127A1 (en) 2004-06-03
EP1572971B1 (de) 2009-09-30
AU2003295576A1 (en) 2004-06-15
WO2004046330A2 (en) 2004-06-03
US7754450B2 (en) 2010-07-13
EP1572971A2 (de) 2005-09-14
WO2004046330A3 (en) 2006-09-28
JP2006526983A (ja) 2006-11-30

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