ATE445454T1 - Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien - Google Patents

Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien

Info

Publication number
ATE445454T1
ATE445454T1 AT02713876T AT02713876T ATE445454T1 AT E445454 T1 ATE445454 T1 AT E445454T1 AT 02713876 T AT02713876 T AT 02713876T AT 02713876 T AT02713876 T AT 02713876T AT E445454 T1 ATE445454 T1 AT E445454T1
Authority
AT
Austria
Prior art keywords
electrophoresis
separation media
media
separation
hydratizable
Prior art date
Application number
AT02713876T
Other languages
English (en)
Inventor
Regina Rooney
Bradley Scoot
Joseph Amshey
Thomas Jackson
Sheldon Engelhorn
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE445454T1 publication Critical patent/ATE445454T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/559—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody through a gel, e.g. Ouchterlony technique
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Electrostatic Separation (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT02713876T 2001-05-10 2002-03-18 Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien ATE445454T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29046401P 2001-05-10 2001-05-10
PCT/US2002/008438 WO2002092200A1 (en) 2001-05-10 2002-03-18 Methods and apparatus for electrophoresis of prior-cast, hydratable separation media

Publications (1)

Publication Number Publication Date
ATE445454T1 true ATE445454T1 (de) 2009-10-15

Family

ID=23116113

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02713876T ATE445454T1 (de) 2001-05-10 2002-03-18 Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien

Country Status (9)

Country Link
US (2) US6936150B2 (de)
EP (1) EP1385603B1 (de)
JP (1) JP4236938B2 (de)
AT (1) ATE445454T1 (de)
CA (1) CA2445539A1 (de)
DE (1) DE60234022D1 (de)
IL (2) IL158298A0 (de)
NZ (1) NZ530022A (de)
WO (1) WO2002092200A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0963435B1 (de) 1997-01-08 2008-05-28 Invitrogen Corporation Verfahren zur herstellung von proteinen
US7517442B1 (en) * 1999-08-09 2009-04-14 Life Technologies Corporation Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats
AUPQ276099A0 (en) * 1999-09-10 1999-10-07 Proteome Systems Ltd Electrophoresis apparatus and a method of using the same
US7601251B2 (en) 2001-05-10 2009-10-13 Life Technologies Corporation Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
JP4236938B2 (ja) * 2001-05-10 2009-03-11 インビトロジェン コーポレイション 予め注型された水和可能分離媒体を電気泳動するための方法および装置
US7622028B2 (en) 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004113899A1 (en) * 2003-06-17 2004-12-29 Invitrogen Corporation Chamber-forming electrophoresis cassette cover
US20070119712A1 (en) * 2003-09-24 2007-05-31 Agilent Technologies, Inc. Electrophoretic separation of amphoteric molecules
EP1668146B1 (de) 2003-09-25 2014-11-12 Life Technologies Corporation Homogene populationen von molekülen
WO2005036153A1 (en) * 2003-10-07 2005-04-21 Invitrogen Corporation Improved isoelectric focusing gels and methods of use thereof
US20050202405A1 (en) 2003-11-28 2005-09-15 Amshey Joseph W. Methods, compositions, and kits for protein crystallization
ATE515325T1 (de) * 2003-12-12 2011-07-15 Becton Dickinson Co Verfahren zur befestigung einer membran mittels heissschmelzen ohne klebstoff
SE0402375D0 (sv) * 2004-09-30 2004-09-30 Amersham Biosciences Ab Method and device for isoelectric focusing
EP1872117B1 (de) 2005-04-15 2017-03-29 Life Technologies Corporation Ausdehnbarer nockenverschluss zur abdichtung von gelplatten in einer elektrophoreseapparatur
EP2069386A4 (de) 2006-07-21 2009-10-28 Life Technologies Corp Proteinmolekülgewichtsstandards mit hoher trennschärfe
JP5008064B2 (ja) * 2006-11-24 2012-08-22 独立行政法人産業技術総合研究所 唾液成分のオンチップ分析方法
US20110100821A1 (en) * 2007-04-20 2011-05-05 Brian Furmanski Mineral oil free isoelectric focusing apparatus for immobilized ph gradient strips
EP2277036A1 (de) * 2008-04-14 2011-01-26 Phorexy Vorrichtung und verfahren für die nicht getauchte gelelektrophorese
US8262889B2 (en) * 2009-02-20 2012-09-11 Bio-Rad Laboratories, Inc. Unitary electrode and electrode support for horizontal electrophoresis
US9494552B2 (en) * 2012-02-07 2016-11-15 Research Foundation Of The City University Of New York Method and system for documenting an electrophoresis gel
EP2986978B1 (de) * 2013-12-02 2019-05-15 Bio-rad Laboratories, Inc. Minigelkamm
US10359396B2 (en) 2015-11-13 2019-07-23 Life Technologies Corporation Preparation of electrophoresis gels, and related devices, systems, and methods
USD856528S1 (en) * 2015-11-13 2019-08-13 Life Technologies Corporation Cassette clamp for electrophoresis gel casting
USD816865S1 (en) 2015-11-13 2018-05-01 Life Technologies Corporation Cassette support base for electrophoresis gel casting
USD851779S1 (en) 2015-11-13 2019-06-18 Life Technologies Corporation Electrophoresis cassette with sample loading guide
USD849963S1 (en) 2015-11-13 2019-05-28 Life Technologies Corporation Electrophoresis gel cassette
USD859688S1 (en) 2015-11-13 2019-09-10 Life Technologies Corporation Electrophoresis slab gel sample loading guide
USD792603S1 (en) 2015-11-13 2017-07-18 Life Technologies Corporation Rig for electrophoresis gel casting
FI127029B (fi) * 2015-12-22 2017-10-13 Outotec Finland Oy Sähkökemiallinen reaktori, vedenkäsittelylaitteisto ja -järjestelmä sekä menetelmä vedenkäsittelylaitteiston ohjaamiseksi

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3875044A (en) 1972-06-09 1975-04-01 Marine Colloids Inc Hydratable gel sheets
US4094759A (en) * 1975-01-03 1978-06-13 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Method for simultaneous quantitative analysis of several constituents in a sample
SE422371B (sv) 1975-12-12 1982-03-01 Aminkemi Ab Sett att generera en ph-funktion for anvendning vid elektrofores
US4337131A (en) 1978-11-13 1982-06-29 C. Desaga Gmbh Nachf. Erich Fecht Process for electrophoresis
US4417967A (en) 1981-11-24 1983-11-29 Georgetown University Grooved gel
US4385974A (en) 1982-06-24 1983-05-31 Jerry Shevitz Electrophoretic system and method for multidimensional analysis
US4443319A (en) 1982-09-30 1984-04-17 E. I. Du Pont De Nemours And Company Device for electrophoresis
US4666581A (en) 1984-05-09 1987-05-19 Hitachi, Ltd. Apparatus for two-dimensional electrophoresis
US4693804A (en) 1984-12-19 1987-09-15 Board Of Regents, The University Of Texas System Apparatus for bidimensional electrophoretic separations
US4746551A (en) * 1985-11-20 1988-05-24 Micro-Map, Inc. Rehydratable polyacrylamide gels
US5275710A (en) 1990-05-14 1994-01-04 Labintelligence, Inc. Gel electrophoresis system including optical stage, sample applicator and sample retriever
US5238651A (en) 1990-07-23 1993-08-24 New York University Gel plates, equipment and kits for combined electrophoretic-immunoelectrophoretic analysis
US5159049A (en) 1991-04-22 1992-10-27 Allen Robert C Method for stabilizing polyacrylamide gels
US5209831A (en) 1991-06-14 1993-05-11 Macconnell William P Bufferless electrophoresis system and method
US5149418A (en) * 1991-09-04 1992-09-22 Apogee Designs, Ltd. Vertical gel electrophoresis apparatus having universal gel assembly support structure
DE4244082C2 (de) * 1992-12-24 1994-11-03 Etc Elektrophorese Technik Wes Verfahren zur hochauflösenden Zweidimensional-Elektrophorese und Vorrichtung zur Durchführung des Verfahrens
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
US5543023A (en) 1995-10-04 1996-08-06 Biotech Holdings, Inc. Gel electrophoresis cassette
US5746901A (en) 1996-04-05 1998-05-05 Regents Of The University Of California Hybrid slab-microchannel gel electrophoresis system
US5800690A (en) 1996-07-03 1998-09-01 Caliper Technologies Corporation Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
US5837116A (en) 1996-07-12 1998-11-17 California Institute Of Technology Two dimensional electrophoresis apparatus
US5699157A (en) 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel
US5827418A (en) * 1996-10-11 1998-10-27 Hoefer Pharmacia Biotech, Inc. Electrophoresis cassette
US5888369A (en) 1997-03-28 1999-03-30 Novel Experimental Technology Apparatus for conducting electrophoresis experiments
US5773645A (en) * 1997-05-05 1998-06-30 Bio-Rad Laboratories, Inc. Two-dimensional electrophoresis device
US5989400A (en) 1997-06-09 1999-11-23 Hoefer Pharmacia Biotech, Inc. Device and method for applying power to gel electrophoresis modules
AUPP108697A0 (en) 1997-12-23 1998-01-22 Macquarie Research Limited Apparatus for electrophoresis
US6001233A (en) 1998-04-13 1999-12-14 Novex Gel electrophoresis apparatus having CAM-activated clamp and methods of use
US6013165A (en) 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
WO2000013007A1 (en) 1998-08-31 2000-03-09 C.B.S. Scientific Co., Inc. Two dimensional gel electrophoresis system
US6156182A (en) 1998-11-19 2000-12-05 Bio-Rad Laboratories, Inc. Encapsulated IPG Strips
DE19930253A1 (de) 1999-06-25 2000-12-28 Wita Gmbh Wittmann Inst Of Tec Kombinationskammer und Verfahren zur Trennung von Biomolekülen
AUPQ276099A0 (en) * 1999-09-10 1999-10-07 Proteome Systems Ltd Electrophoresis apparatus and a method of using the same
AUPR051500A0 (en) 2000-09-29 2000-10-26 Proteome Systems Ltd Electrophoresis system
JP4236938B2 (ja) * 2001-05-10 2009-03-11 インビトロジェン コーポレイション 予め注型された水和可能分離媒体を電気泳動するための方法および装置

Also Published As

Publication number Publication date
NZ530022A (en) 2005-09-30
IL165848A0 (en) 2006-01-15
IL158298A0 (en) 2004-05-12
EP1385603A1 (de) 2004-02-04
US20030015426A1 (en) 2003-01-23
US20050284764A1 (en) 2005-12-29
CA2445539A1 (en) 2002-11-21
EP1385603B1 (de) 2009-10-14
WO2002092200A1 (en) 2002-11-21
JP4236938B2 (ja) 2009-03-11
EP1385603A4 (de) 2005-01-12
WO2002092200A8 (en) 2004-02-05
JP2004535565A (ja) 2004-11-25
US6936150B2 (en) 2005-08-30
DE60234022D1 (de) 2009-11-26

Similar Documents

Publication Publication Date Title
ATE445454T1 (de) Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien
WO2001071732A3 (de) Porous ferro- or ferrimagnetic glass particles for isolating molecules
ATE454463T1 (de) Überexpression von extremozymgenen in pseudomonas und nahe verwandte bakterien
ATE520466T1 (de) Apparat und verfahren zur modifizierung von magnetisch inmmobilizierte biomolekulen
WO2004025252A3 (en) Automated system for high-throughput electrophoretic separations
ATE242780T1 (de) Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür
ATE474915T1 (de) Verfahren zur induktion der entwicklung und terminalen differenzierung von gedächtnis-b- zellen
DE60129000D1 (de) Verfahren und mittel zur Isolierung von Mikroorganismen
DE69800630D1 (de) Chip zur elektroforetischen Trennung von Molekülen und Verfahren zur Verwendung desselben
ATE408422T1 (de) Methode zur hemmung immunstimulatorischer mit dna assoziierter antworten
WO2000067007A3 (en) Method and apparatus for obtaining electric field-enhanced bioconjugation
NO20034484L (no) Anordning for styring og/eller utproving av et undervanns hydrokarbonproduksjonssystem.
DE69232682D1 (de) Verfahren zur selektiven vermehrung von cd34 positiven zellen
NO20023114D0 (no) Fremgangsmåte og system for migrering av seismiske data
EP0871645A4 (de) Vorrichtung und verfahren zur geteilten behandlung von abwasser
ATE336806T1 (de) Verfahren und vorrichtung zum entionisieren von k hlmedien f r brennstoffzellen
MXPA03004006A (es) Proceso para la tipificacion rapida de microorganismos y juego de reactivos empleado.
WO2004081694A8 (fr) Procede pour l'automatisation de la mise en oeuvre et la mise a jour d'un systeme d'information
DE60124780D1 (de) Vorrichtung und verfahren zur serienvermehrung von säugerzellen zum animpfen
ATE483724T1 (de) Verfahren zur erhöhung der ausbreitung von b- zellen
SG98496A1 (en) Method for ascertaining the status of information system, and apparatus to be used with the method
DE602005015716D1 (de) Bildrandfilterung
DE60210607D1 (de) Nährboden zum nachweis und/oder zur unterscheidung von enterokokken
DE602005026412D1 (de) Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten
FI20012319A0 (fi) Menetelmä ja laitteisto radiokanavan simuloimiseksi

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties